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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
Editor in Chief :
Volume & Issues
Volume 2, Issue 4 - Dec 2007
Volume 2, Issue 3 - Sep 2007
Volume 2, Issue 2 - Jun 2007
Volume 2, Issue 1 - Mar 2007
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Research and Development of Marine Bio-Organisms - Introduction and Its prospect -
Han, Sangtae ; Kim, Jong-Man ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 73~76
A large number of natural products were worldwidely discovered from maine organisms. There are many secondary metabolites produced by maine organisms which showed very strong bio-activities and distinct bio-active mechanisms. In korea, about 350 secondary metabolites including more than 280 novel compounds have been isolated and structually defined. Many researches for developing products such as cosmetics, functional food materials, anti-fouling substances are being performed using distinct activities of marine organisms. Although the research on marine natural products has been remained at its initial or developing stage of drug development, significant progress has been made for isolation, structure determination and bioassay of secondary metabolites.
Macroalgae as the Source for Environmental Assessment
Luyen, Hai-Quoc ; Meinita, Maria D.N. ; Hong, Yong-Ki ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 77~80
Macroalgal tissues can be used as indicating materials for environmental assessment using several algal biotechnology techniques. As bioassay test organisms, macroalgal tissues are required as an axenic state for suitable biological indicators. Callus formation and blade regeneration under suitable culture conditions are also useful for the tests. Quantitative method using tetrazolium chloride or
is devised on a rapid assessment of the seaweed viability. The use of RT-PCR especially differential display technique should provide the means for the detection and isolation of the responding genes induced by the environmental stress. Seaweed thriving in more environmental changes might contain more diverse biologically active substances.
In vitro Analysis of Glucocorticoid-induced Reporter Gene Expression Using Lentivirus System
Lee, Mi-Sook ; Kim, Ji-Yeon ; Her, Song ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 81~85
Glucocorticoid hormone regulates numerous physiological processes, such as regulation of metabolism, and anti-inflammatory and immunosuppressive actions via the activation and repression of gene expression. Here we described a lentivirus-based reporter vector system expressing red fluorescent protein (mRFP) or firefly luciferase (Luc) under the control of a glucocorticoid-responsive element that allows observation of the temporospatial pattern of glucocorticoid induced GR-mediated signaling on a cellular level. Moreover, usage of the chromatin insulator of the chicken
-globin locus induced a marked increase of sensitivity of glucocorticoid inducible promoter of a reporter gene. Use of this method will be applicable of screening for agonist and antagonist of GR in vitro, and also a reporter gene assay for the in vivo determination of the GR-mediated gene activation.
Therapeutic Potential of Ethanolic Extract of Ecklonia cava on Inhibition of Inflammation
Kim, Moon-Moo ; Lee, Sang-Hoon ; Ngo, Dai-Nghiep ; Jung, Won-Kyo ; Kim, Se-Kwon ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 86~93
Chronic inflammation has been known to have a close relationship with several diseases including periodontitis, colitis, hepatitis and arthritis. Recently anti-inflammatory agents have been developed from marine natural resources. In this study, Ecklonia cava (EC) was found to have anti-inflammatory effect. Ethanolic extract of EC belonging to brown algae exhibited an excellent inhibitory effect on the production of inflammatory mediators such as tumor necrosis factor-
, interleukin-6 and prostaglandin
by RA W264.7 cells. Furthermore, in reporter gene assay and western blot analysis, EC extract exerted anti-inflammatory effect via inactivation of NF-
transcription factor that regulates the expression of these inflammatory mediators in macrophages. In addition, EC extract inhibited the activity of matrix metalloproteinase that play an important role in chronic inflammation. These results suggest that EC extract may provide a pharmaceutical potential in inhibiting chronic inflammation.
Pangasiid Catfish Pangasius hypophthalmus Farming in Bangladesh: a Rural Survey in the Mymensingh Region
Sarkar, Reaz Uddin Md. ; Khan, Saleha ; Haque, Mahfuzul Md. ; Khan, Mohammed Nurul Absar ; Choi, Jae-Suk ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 94~101
The status of recently expanded exotic pangasiid catfish Pangasius hypophthalmus, Pangasiidae farming in rural Bangladesh has been studied for finding research needs, through knowing the culture methods, associated activities and problems, to make the farming sustainable. Data were collected using participatory rural appraisal (PRA) tools in nine villages in three upazilas (sub-district) of Mymensingh district. The farmers have not got any formal training and have developed their knowledge on farming the fish through practice over years and sharing of knowledge among fellow farmers. Linked industries, e.g. hatcheries, nurseries, feed mills, trading of feed and fish etc. have developed that created employment opportunities. Most of the farmers produce two crops a year. About 90% of the farmers were found to practice mono culture of pangasiid catfish at high stocking density. The feeding rates started from 10-15% of the body weight per day at fingerling stage that reduced to 4-6% with growth. The average yield was found to be 25 tonslha/year. The livelihood of the farmers has been improved through farming the fish. Women and children were rarely engaged in the activities. The identified major problems in farming the fish were water quality deterioration, high feed costs and declining consumer demand and market price.
Effects of Chitosan on the Toxicity of Environmental Pollutants
Lee, Hyon-Gyo ; Kim, Hae-Young ; Yang, Jae-Ho ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 102~107
Environmental contamination becomes a great public concern as our society gets industrialized rapidly. The present study examine the role of chitosan in a effort to intervene the environmental pollutant-induced toxicity. PCB-induced neurotoxicity with respect to the PKC signaling was examined. Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old SD rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. PCB showed the alteration of PKC signaling pathway. The alteration was structure-dependent. Mono-ortho-substituted congeners at a high dose showed a significant increase of total PKC activity at [
]PDBu binding assay, indicating that mono-ortho-substituted congeners are more neuroactive than non-ortho-substituted congeners in neuronal cells. PKC isoforms were immunoblotted with respective monoclonal antibodies. PKC-beta II and -epsilon were activated with mono-ortho-substituted congeners exposure. The result suggests that the position with ortho has a higher potential of altering the signaling pathway. Alteration of PKC was blocked with treatment of high molecular weight of chitosan. The study demonstrated that the ortho position in PCBs are important in assessing the structure-activity relationship. The results suggest a potential use of marine bioactive materials as a means of nutritional intervention to prevent the harmful effects of pollutant-derived toxicity.
Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli
Park, Eun-Mi ; Han, Yun-Hee ; Park, In-Suk ; Nam, Bo-Hye ; Kong, Hee Jeong ; Kim, Woo-Jin ; Lee, Sang-Jun ; Kim, Young-Ok ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 108~112
A cellulase (endo-
-1,4-D-glucanase(E.C.18.104.22.168)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.
The Antimicrobial and Growth Inhibitory Effects of Gelidium amansii L. Fractions on Cancer Cell Lines
Shin, Hye-Jung ; Gang, Dae-Yeon ; Shin, Mi-Ok ; Bae, Song-Ja ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 113~119
In this study, we investigated antimicrobial and cytotoxicity effects of Gelidium amansii L., which using methanol, dichloromethane and ethanol were extracted and fractionated into four different types : methanol (GAMM), hexane (GAMH), butanol (GAME) and aqueous (GAMA). The antimicrobial activity was increased in proportion to its concentration by the paper disc method. Among the solvent fractions, The methanol partition layer (GAMM) showed the strongest antimicrobial activities and cytotoxic effects on all cancer cell lines. We also observed quinone reductase (QR) induced effects in all fraction layers of GA on HepG2 cells. The QR induced effects of GAMM on HepG2 cells at
concentration indicated 2.5 with a control value of 1.0.
Isolation and Identification of a Photosynthetic Bacterium Containing
Jeong, Soo Kyoung ; Kim, Joong Kyun ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 120~122
-producing photosynthetic bacterium was isolated from the silt at Nakdong river. The isolate had 1.55 mg of
per gram of dry cell. By the 16s-rDNA sequence analysis, the isolate was found to be Rhodobacter sphaeroids with 100% similarity (Genbank Accession No.=AM696701).
Isolation and characterization of the outer membrane vesicle (OMV) protein from Vibrio anguillarum O1
Hong, Gyeong-Eun ; Kim, Dong-Gyun ; Min, Mun-Kyeong ; Kong, In-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 2, 2007, Pages 123~125
Vibrio anguillarum is a gram-negative bacterium that causes vibriosis in approximately 80 different fish species. V. anguillarum produces several exotoxins are correlated with the pathogenesis of vibriosis. This study is focused on the composition of the outer membrane vesicle. Most of gram-negative bacteria produce outer membrane vesicle (OMV) during cell growth. OMV was formed from the outer membrane surface of cell and than released to extracellular environment. OMV consists of outer membrane lipids, outer membrane protein (OMP), LPS, and soluble periplasmic components. Also, they contain toxins, adhesions, and immunomodulatory. Many gram-negative bacteria were studied out forming OMV. In Vibrio sp., formation of OMV by electron microscopy has been reported from V. cholerae and V. parahaemolyticus. In present study, we isolated OMV from V. anguillarum and OMV protein was separated by SDS-PAGE. Magor band was sliced and analyzed by MALDI-TOF. The major protein band of 38kDa was identified as OmpU by MALDI-TOF MS analysis.