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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
Editor in Chief :
Volume & Issues
Volume 2, Issue 4 - Dec 2007
Volume 2, Issue 3 - Sep 2007
Volume 2, Issue 2 - Jun 2007
Volume 2, Issue 1 - Mar 2007
Selecting the target year
Innate Immune Response of NNV Infection in Fish and Its Disease Prevention
Lu, Ming-Wei ; Wu, Jen-Leih ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 127~132
The innate immune response which is seen as the initial defense mechanism induced upon foreign invasion has been well documented in higher vertebrates. This has also been observed in fish infected with NNV. However, the fish immune system based on fully established genome project has not been fully elucidated. Therefore, in this review, we hope to correlate NNV infection in fish that has devastated the aquaculture industry, to its host immune system. Further, we discuss the potential preventive measures in overcoming the widespread of this neurodisease.
Development of Value-Added Products Using Seaweeds
Park, Yang-Kyun ; Kang, Seong-Gook ; Jung, Soon-Teck ; Kim, Dong-Han ; Kim, Seon-Jae ; Pak, Jae-In ; Kim, Chang-Hyeug ; Rhim, Jong-Whan ; Kim, Jung-Mook ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 133~141
There are produced more than 600,000 tons of seaweeds every year along the coast of the Korea. Jeonnam province, south-west coast area, of Korea is producing 93% of total amounts of seaweeds. The laver, sea mustard, and tangleweed maintain stability in the output and has been exported as a simple product processing through drying or salting. It was evaluated the low value-added products and limited the expansion for the consumption of seaweeds. The seaweeds contains 40-60% carbohydrate and structurally different compared with land plant. The dietary fiber from seaweeds has been known the function of facilitating the bowl movement, excretion the heavy metal in the body, lowering the blood cholesterol level, anti-coagulant of blood, and anticancer. Especially, brown algae including sea mustard, seaweed fusiforme, and tangleweed contains alginic acid, laminarin, mannitol, fucoidan which are lowering the blood cholesterol level, lowering blood pressure, and fusion of blood clot. Agar-agar, carrageenan, and porphyran compound in red algae are known to antimutagenicity and anticoagulant function. In spite of potential of seaweed as a main bio-resource, there are lack of research to facilitate the consumption with its functional properties and consumers are unsatisfied with simple processing products. Also, the seaweed by-product dump into the sea and cause pollution of the seawater. Therefore, there are needed the scheme to promote the consumption of seaweeds. The development of value-added products, finding functional properties from seaweeds, development the functional feed for animal using seaweed by-products, and utilization of unused algae for food or other industrial uses will increase fisherman's income as well as serve as an aid for the people health due to its functional properties. Using by-product of seaweed and unexploited seaweed are needed to development of bio-degradable food packaging material and functional feed for animal.
Purification and Characterization of Agarase from Marine Bacterium, Algibacter lectus AS-3
Jung, Il Sun ; Choi, Young Ju ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 142~148
An agar-degrading marine bacterium, strain AS-3 was isolated from the seawater. The strain AS-3 was identified as Algibacter lectus AS-3 by 16S rDNA sequence. The optimum medium for agarase activity of the isolated strain was determined to be marine medium, marine broth 2216 containing 0.1% agar as carbon source. An extracellular agarase was purified 6.9-fold from the culture supernatant by ammonium sulfate precipitation, ion exchange chromatography and gel filtration methods. The optimum pH and temperature for this enzyme were 7.0 and
, respectively. Antioxidative activity of the strain AS-3 was 62.4% in the supernatant cultured for 12 h.
Hot Water Extraction of Crude Chondroitin Sulfate from Shark Cartilage Treated with Ozone Water
Jung, Yoo-Kyung ; Shin, Kyung-Ok ; Shin, Jong-Wook ; No, Hong-Kyoon ; Kim, Soon-Dong ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 149~154
This study was conducted to investigate the effect of ozone treatment on removal of brown color and ammonia-like odor produced from shark cartilage (SC) and to establish the optimal extraction conditions of chondroitin sulfate (CS) by NaOH and hot water. Treatment of SC with 0.3 ppm ozone water resulted in higher color
value but lower
values compared with those of non-treated SC, without affecting its proximate compositions (moisture, protein, lipid, carbohydrate, and ash). Ozone treatment also increased the overall acceptability of SC by reducing ammonia-like odor. The optimum concentration and time for extraction of CS from SC using NaOH at
were 1 N and 120 min, respectively, with 10.02% of extraction yield. The total yield (10.01%) of CS obtained by 7 times repeated extraction with hot water at
was comparable to that of the 1 N NaOH extraction for 120 min at
Study for Alanalysis and Characteristics of Squid Viscera Oil During Transesterification
Roh, Myong-Kyun ; Uddin, Salim ; Chun, Byung-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 155~159
Ethanolysis of squid viscera oil with immobilized lipase was investigated for reducing the free fatty acid contents and enhancing the function of the oil by stepwise addition method of Shinmada. Tendency of oil variation during Ethanolysis showed increased content of diglyceride, monoglyceride and fatty acid ethyl ester with reduced free fatty acid contents. The oil composition was analyzed using GC-FID and compared before and after ethanolysis. Structural analysis of the lipid was performed by HPLC-UV spectrophotometer during ethanolysis. The transformed oil was thought to has suitable properties for functional oil production.
Quality Factor Determination and Shelf-Life Prediction of Powdered-Model Food
Baik, Eun-Kyung ; Park, Seok-Jun ; Lee, Kang-Pyo ; Choi, Sung-Won ; Hur, Nam-Yun ; Baik, Moo-Yeol ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 160~167
Shelf-stability of powdered model food was determined during storage at various temperatures (
) and various moisture contents (3.5%, 6.0%, 8.0%). Moisture content, peroxide value, pH, color, microbial counting and sensory evaluation were conducted during storage. Moisture content, peroxide value, pH and color were not significantly changed during storage in all samples indicating that this powdered model food was relatively stable at given conditions. Pathogenic microorganisms, such as Bacillus cereus, Listeria spp., Clostridium perfrigens, Salmonella spp. and Staphylococcus aureus, were not found during storage suggesting that there was no problem in safety in this case. On the other hand, the number of artificially added Lactic acid bacteria was decreased with increasing both storage temperature and moisture content. Therefore, powdered model food was very shelf-stable and it was impossible to predict the shelf-life using above quality factors.
EST-based Identification of Genes Expressed in the Muscle of Olive Flounder, Paralichthys olivaceus
Park, Eun-Mi ; Kim, Young-Ok ; Nam, Bo-Hye ; Kong, Hee Jeong ; Kim, Woo-Jin ; Lee, Sang-Jun ; Choi, Tae-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 168~173
of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics. To analyze the transcriptome of olive flounder, Paralichthys olivaceus, we have conducted EST analysis using cDNA libraries made from muscle of P. olivaceus. Redundant ESTs were assembled into overlapping contigs by using the assembly program ICAtools software. We found that the 221 ESTs were composed of 21 clusters and 35 singletons, suggesting that the overall redundancy of the library was 74.7%. Of the 221 clones, 218 clones (98.6%) were identified as known genes by BLAST searches and 3 clones (1.4%) did not match to any previously described genes. Based on major functions of their encoded proteins, the identified clones were classified into 13 broad categories. Sequence analysis of the ESTs revealed the presence of microsatellite-containing genes which may be valuable for further gene mapping studies. This study contributes to the identification of many EST clones that could be useful for genetics and developmental biology of olive flounder.
Growth Characteristic, Mono-strain Mass Culture and Antioxidant Effects of Two Benthic Diatoms Amphora coffeaeformis and Achnanthes longipes from Korea
Abu Affan, Md. ; Karawita, Rohan ; Jeon, You-Jin ; Lee, Joon-Baek ; Kang, Do-Hyung ; Park, Heung-Sik ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 174~186
Amphora coffeaeformis and Achnanthes longipes are commonly found as dominant benthic microalgae in Jeju coastal water throughout the year. In order to investigate pharmaceutical uses of these diatoms, each single species was isolated with micropipette under phase contrast microscope and subcultured with synthetic seawater media which was enriched with F/2 media, trace metal solution and
). Growth characteristics of these species were also determined with different combination of salinity, nutrients concentration and temperature. Thereafter, mass culture of each species was done based on the maximum growth condition. Biomass was collected after two weeks of mass culture and freeze dried for antioxidant study. The antioxidant properties of different fractions (n-hexane, chloroform and ethylacetate) obtained by solvent fractionation of 80% methanolic extract of two microalgae were investigated for free radical, reactive oxygen species scavenging (Super oxide, Hydrogen peroxide, Hydroxyl radical and Nitric oxide), metal chelating and lipid peroxidation inhibition activities. All fractions of A. longipes showed higher
(free radical) scavenging activities (n-hexane: 89.0%, Chloroform: 76.0%, Ethylacetate: 66.0%, Methanol: 90.6% and aqueous residue: 63.0%). N-hexane fraction of A. longipes showed significantly higher activity (49.0%) on nitric-oxide. Ethylacetate fraction of A. longipes and aqueous residue of A. coffeaeformis exhibited 64.0% and 75.6% metal chelating activity which was higher than commercial antioxidants (
-tocopherol: 18.0% and BHT: 16.0%). The n-hexane fraction of A. coffeaeformis had 67.5% activity on
. Chloroform and n-hexane fractions of A. coffeaeformis exhibited 46.2% and 47.6%
scavenging effects which were closely similar to commercial antioxidants (
-tocopherol: 49.2% and BHT: 58.6%). Chloroform and ethylacetate fractions of A. longipes and fraction of n-hexane and chloroform of A. coffeaeformis showed better lipid peroxidation activities than
-tocopherol. These data suggest that both organic and aqueous fractions have good antioxidative compounds with different antioxidant properties.
Radical Scavenging Effect of Methanol Extracts from Seaweeds and Their Active Compounds
So, Mi Jung ; Cho, Eun Ju ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 187~191
The radical scavenging activity of methanol extracts of seaweeds and their active compounds, alginic acid, fucoidan and phloroglucinol, were investigated under in vitro. Among methanol extracts of seaweeds (sea mustard, sea tangle, seaweed papulosa, fusiforme, sea lettuce, purple laver and chlorella), seaweed papulosa and sea tangle showed strong scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical (
). In addition, under in vitro, the scavenging activities on DPPH radical of alginic acid and fucoidan, which are active compounds of brown algae, and phloroglucinol, the active compound from Ecklonia species, were evaluated and compared. Fucoidan and phloroglucinol showed strong DPPH scavenging effect, in particular, phloroglucinol had strongest activity among the active compounds. On the other hand, alginic acid did not exert DPPH scavenging activity. From the present study, we could confirm the antioxidative activity of seaweeds and its active compounds.
Gene Expression Profiles in the Peripheral Blood Leukocytes (PBL) of Olive Flounder Paralichthys olivaceus after Stimulation with Lipoolysaccharide (LPS) in vitro
Nam, Bo-Hye ; Moon, Ji-Young ; Kim, Young-Ok ; Kim, Woo Jin ; Kong, Hee Jeong ; Lee, Sang-Jun ; Choi, Tae-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 192~197
Suppression subtractive hybridization (SSH) was performed to construct an cDNA library of olive flounder Paralichthys olivaceus peripheral blood leukocytes (PBL) stimulated with lipopolysaccharide (LPS). Total 470 clones were randomly selected and sequenced, 214 out of 470 sequences showed identities with known genes and 252 sequences were unknown genes. Among the 218 known genes, 34 sequences were found to be homologous to IL-1RII gene, and 14 sequences were identified as IL-
. RT-PCR analysis showed that IL-
mRNA was induced 1h after LPS-stimulation, and IL-1RII was increased from 3h after stimulation, indicating that most of SSH clones are associated with inflammatory responses in fish, and this SSH cDNA library would be useful to identify bio-defense and immuno-related genes in fish.
Reaction Characterization of a Photosynthetic Bacterium Containing
Jeong, Soo Kyoung ; Kim, Joong Kyun ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 3, 2007, Pages 198~200
Cultivation of a
-producing photosynthetic bacterium, Rhodobacter sphaeroids, was carried out in a 1-L flask to characterize its cellular growth reaction. The result of experiment showed that dissolve oxygen in the broth was depleted within 7 h. ORP decreased with decrease of DO, and recovered somewhat with increase of pH. The growth of R. spahaeroids reached at late-log phase in 140 h of reaction. The final pH and dry-cell weight were 7.62 and 2.2 mg/mL, respectively. The
content in the final broth was 2.35 mg/g dry cell weight, which was higher than that obtained in tube culture.