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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
Editor in Chief :
Volume & Issues
Volume 2, Issue 4 - Dec 2007
Volume 2, Issue 3 - Sep 2007
Volume 2, Issue 2 - Jun 2007
Volume 2, Issue 1 - Mar 2007
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Polychlorinated Biphenyls (PCBs) in the Bio-geochemistry of Oceans
Kannan, Narayanan ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 201~208
Polychlorinated biphenyls (PCBs) are anthropogenic contaminants found globally in water, ice, soil, air and sediment. Modern analytical techniques allow us to determine these chemicals in environmental matrices at parts per trillion levels or lower. Environmental forensic on PCBs opens up new avenues of investigation such as transport and fate of water masses in oceans, sedimentation, onset of primary production, migration of marine mammals, their population distribution and pharmacokinetics of drugs inside organisms. By virtue of persistence, bioaccumulation, bioconcentration and structure-activity relationship PCBs emerge as unconventional chemical tracers of new sort.
Bioactive Marine Natural Products in Drug Development
Kim, Se-Kwon ; Ravichandran, Y. Dominic ; Kim, Moon-Moo ; Jung, Won-Kyo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 209~223
Nature is one of the most important sources of pharmacologically active compounds in the search for drugs against life threatening diseases. Even though plants and terrestrial microorganisms have played as an important source for the new drug candidates from nature, marine organisms such as tunicates, sponges, soft corals, sea horses, sea snakes, marine mollusks, seaweeds, nudibranches, sea slugs and marine microorganisms are increasingly attracting attention in recent years. Marine organisms also have the potential to develop into future drugs against important diseases, such as cancer, a range of bacterial and viral diseases, malaria, and inflammations. Even though the mechanism of action in the molecular level of most metabolites is still unclear, the mechanisms by which they interfere with the pathogenesis of a wide range of diseases have been reported. The knowledge of this is one of the key factors necessary to develop bioactive compounds into medicines. This is due to their structurally unique and pharmacologically active compounds. The potential pharmaceutical, medicinal and research applications of some of these compounds are discussed in hundreds of scientific papers, and are reviewed here.
Characterization and Expression Pattern of the Partial Myostatin cDNA in Shrimp, Fenneropenaeus chinensis
Lee, Sang Beum ; Kim, Yong Soo ; Yoon, Moongeun ; Kim, Su-Kyoung ; Jang, In Kwon ; Lim, Hyun Jeong ; Jin, Hyung-Joo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 224~229
Muscle tissue expresses many muscle-specific genes, including myostatin (also known as GDF8) that is a member of the transforming growth factor-beta superfamily. Myostatin (MSTN) negatively regulates mammalian skeletal muscle growth and development by inhibiting myoblast proliferation. Mice and cattle possessing mutant MSTN alleles display a 'double muscling' phenotype characterized by extreme skeletal muscle hypertrophy and/or hyperplasia. In this study, we first have characterized partial cDNA of a MSTN gene from the muscle tissue in the F. chinensis and examined its expression pattern in various tissues. The partial MSTN gene (GenBank accession number EU 131093) in the F. chinensis was 1134 bp, encoding for 377 amino acids that showed 63-93% amino acid similarity to other vertebrate MSTNs, containing a conserved proteolytic cleavage site (RXRR) and conserved cysteine residues in the C-terminus. Based on a RT-PCR, the MSTN gene was expressed in the all tissues of F. chinensis used in this study.
Screening of Marine Microbial Extracts for Tyrosine Phosphatase 1B Inhibitors
Sohn, Jae-Hak ; Park, Sun Jung ; Seo, Changon ; Chun, Bokyung ; Oh, Hyuncheol ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 230~233
Protein tyrosine phosphatase 1B (PTP1B) acts as a negative regulator of insulin signaling, and selective inhibition of PTP1B has served as a potential drug target for the treatment of type 2 diabetes. As part of our searching for PTP1B inhibitors from natural products, the extracts of marine microorganisms were screened for the inhibitory effects on the activity of protein tyrosine phosphatase 1B (PTP1B). Among the tested 304 extracts, 29 extracts exhibited inhibition rate ranging 40.1 - 83.6 % against PTP1B at the concentration level of
Calmodulin of Olive Flounder Paralichthys olivaceus : Cloning and Expression Analysis
Hong, Gyeong-Eun ; Kong, Hee Jeong ; Nam, Bo-Hye ; Kim, Young-Ok ; Kim, Woo-Jin ; Lee, Sang-Jun ; Choi, Tae-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 234~237
Calmodulin (CaM) is a
-binding protein essential for biological functions mediated through
-dependent mechanism. A cDNA clone for CaM was isolated from a cDNA library of olive flounder Paralichthys olivaceus. The CaM cDNA concists of 782 bp and encodes a polypeptide of 149 amino acids with four
-binding motifs EF-hands (EF-I, EF-II, EF-III, and EF-IV). The deduced amino acid sequence of CaM shows 97-100% amino acid sequence identity to other CaM sequences. Semi-quantitative PCR analysis revealed that the CaM transcription was began during early development and the CaM mRNA is expressed highly in brain and intestine, and moderately in kidney, gill, and eye of healthy olive flounder. Taken together, CaM may be necessary for early olive flounder development and that it may have a part in homeostasis.
PCR-based Identification of Microorganisms in a Kefir Grain
Koo, Won Hoe ; Seo, Min-Gook ; Ahn, Jung Hoon ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 238~244
Nowadays many people are concerned about being healthy, and many dairy products are taken as health supplementary foods. Among dairy products, kefir, also called as Tibet mushroom, is a yogurt fermented by kefir grain, which is a mixture of lactic acid bacteria and yeasts. Although there are many empirical evidences that kefir is very influential for human body, the exact reason is not definitively discovered. Therefore, it would be useful to understand characteristics of a kefir grain and to categorize bacteria in a kefir grain. In this paper, molecular biological apparatus such as PCR, electrophoresis, PCR purification, DNA sequencing were used to identify and classify the species of lactic acid bacteria and yeast in a kefir grain. We used PCR-based identification method using 16S rRNA primer and Internal Transcribed Spacer (ITS) primer. We identified 6 different species which were selected on different medium. In addition, observation with scanning electron microscope (SEM) enabled us to grasp an external shape of the kefir grain. Although we found a limited number of microbial species, more intensive research are needed for extensive identification of microorganism species in Korean kefir grain.
Methods for High Quality Purification of Alginate and Fucoidan from Marine Brown Algae
Kim, Jong-Ki ; Yang, Jae-Ho ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 245~249
High quality of purified alginate and fucoidan is required for the medical uses to prevent the unexpected side effects from the contaminated endotoxin present in the materials. We attempted to establish an efficient and fast mass scale production method for the highly purified poly-G alginate from the sea weeds. In addition, lab scale method was established to obtain the fraction of higher purity from the commercially available fucoidan source (about 85% purity).
Inhibition of Cell Adhesion by Noncyclic Triterpenoids from Alpinia katsumadai
Kim, Ji-Soo ; Rho, Mun-Chual ; Kim, Gang-Seong ; Kim, Koanhoi ; Rhim, Byung-Yong ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 250~256
The present study purified two noncyclic triterpenoids, compound 1 and compound 2, that intervene interaction of ICAM-1 and LFA-1 from ethanol extracts of Alpinia katsumadai. The compound 1 and 2 inhibited adherence of sICAM-1 to THP-1 cells with an
, respectively, without affecting viability of the cells. The compound 1 and 2 also inhibited interaction of CHO-ICAM-1 cells with THP-1 cells with an
, respectively. These findings suggest that the noncyclic triterpenoids from Alpinia katsumadai have inhibitory activities against cell adherent molecules. The present study proposes that noncyclic triterpenoids from Alpinia katsumadai can applied to therapeutic approaches to the diseases that are associated with adhesion of inflammatory cells.
Study for Improving Properties of Squid Viscera Oil Using Transesterification and Adsorption
Roh, Myong-Kyun ; Uddin, Salim ; Chun, Byung-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 257~262
Squid viscera oil was investigated by pretreatment method for enhancing the commercial value. Transeterification was performed to reduce rancidity of the oil, off-flavor was removed by using activated carbon adsorption. Analysis using ATD (Automatic Thermal Desorber) and GC/MG shows the efficacy of off-flavor removement. The rates of Transesterification employing inorganic catalyst and biocatalyst were tested, respectively. With stepwise addition of ethanol, the most efficiency of the reaction was achieved by inorganic catalyst. The efficiency of the reaction was estimated by acid value corresponding to rancidity of reaction product.
Detection of Vibrio vulnificus by Real-Time PCR targeted to rpoS gene
Kim, Dong-Gyun ; Ahn, Sun-Hee ; Bae, Ju-Yoon ; Kong, In-Soo ;
Journal of Marine Bioscience and Biotechnology, volume 2, issue 4, 2007, Pages 263~266
Vibrio vulnificusis a causative agent of serious diseases in humans resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately
for V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or
V. vulnificus cells from pure cultured broth and
cells in 1g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium using the rpoS gene in pure cultures and in infected oyster tissues.