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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
Editor in Chief :
Volume & Issues
Volume 6, Issue 2 - Dec 2014
Volume 6, Issue 1 - Jun 2014
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Detecting Techniques for Marine-derived Pathogens: Grouping and Summary
Hwang, Byeong Hee ; Cha, Hyung Joon ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 1, 2014, Pages 1~7
DOI : 10.15433/ksmb.2014.6.1.001
Marine-derived pathogens threat health and life of human and animals. Therefore, rapid and specific detection methods need to be developed. Here, we summarized various groups of detection methods, including conventional method, flow cytometry, nucleic acid-based method, and protein-based method. In addition, perspective of detection technique was discussed as a unified detection system for pathogens.
Biofuel production from macroalgae toward bio-based economy
Lim, Hyun Gyu ; Kwak, Donghun ; Jung, Gyoo Yeol ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 1, 2014, Pages 8~16
DOI : 10.15433/ksmb.2014.6.1.008
Macroalgae has been strongly touted as an alternative biomass for biofuel production due to its higher photosynthetic efficiency, carbon fixation rate, and growth rate compared to conventional cellulosic plants. However, its unique carbohydrate composition and structure limits the utilization efficiency by conventional microorganisms, resulting in reduced growth rates and lower productivity. Nevertheless, recent studies have shown that it is possible to enable microorganisms to utilize various sugars from seaweeds and to produce some energy chemicals such as methane, ethanol, etc. This paper introduces the basic information on macroalgae and the overall conversion process from harvest to production of biofuels. Especially, we will review the successful efforts on microbial engineering through metabolic engineering and synthetic biology to utilize carbon sources from red and brown seaweed.
Clay-based Management for Removal of Harmful Red Tides in Korea: A Multi-perspective Approach
Choi, Moon-Hee ; Lee, Soon Chang ; Oh, You-Kwan ; Lee, Hyun Uk ; Lee, Young-Chul ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 1, 2014, Pages 17~25
DOI : 10.15433/ksmb.2014.6.1.017
Periodically, harmful algal blooms (HABs) have occurred, with impacts on various areas including public health, tourism, and aquatic ecosystems, especially aquacultured and caged fisheries. To prevent or manage invasions of HABs into fish farms on an emergency basis, many methods have been proposed. Frequently over the past 30 years in coastal countries, treatments of clay and clay mixed with polyaluminum chloride (PAC) and chitosan have been tested for HAB-removal effectiveness in both the laboratory and the field. In Korea, yellow loess clay (hwangto) has been dispersed using electrolytic clay dispensers, both to decrease the amount of yellow loess clay's usage in containers and enhance HAB-removal efficiency. However, this emergency method has limitations, among which is the requirement for more effective controlling agents for field applications. Thus, in this paper, we review technologies for clay-based red tides prevention and control and their limitations, and, further, introduce next-generation algicidal technologies for the emergency protection of fish farms.
Isolation of Lipid High-yielding Chlorella vulgaris Mutants by UV Irradiation
Jeong, Haeng Soon ; Choi, Min Kyung ; Choi, Tae-O ; Lee, Jae-Hwa ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 1, 2014, Pages 26~30
DOI : 10.15433/ksmb.2014.6.1.026
Chlorella vulgaris, a genus of single-cell green algae, is considered to be a very essential resource for the higher value-added business including functional food and biodiesel, due to its high contents of protein, carbohydrate and lipid. In this study, ultraviolet rays were irradiated in order to induce the mutation of C. vulgaris. After inducing the mutation, UV1-20 mutant, high in lipid was selected and its cell growth rate, dry weight, pigment content and lipid content were measured. The growth rate of the UV1-20 mutant was increased almost 1.5 times than the wild type, but pigment contents of chlorophyll and carotinoid were decreased. In addition, the lipid content of UV1-20 was increased 1.8 times than the wild type. Therefore, C. vulgaris mutant, isolated in this study, is considered to have sufficient potential to be used as a material for the higher value-added business.
Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus
Kim, Sun-Hyoung ; Jeong, Sung Eun ; Hong, Seong-Joo ; Lee, Choul-Gyun ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 1, 2014, Pages 31~40
DOI : 10.15433/ksmb.2014.6.1.031
The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design
in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be
light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.