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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Marine Bioscience and Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society for Marine Biotechnology
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Volume & Issues
Volume 6, Issue 2 - Dec 2014
Volume 6, Issue 1 - Jun 2014
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An ultrastructural study of the cuticle in the byssus of marine mussel (Mytilus coruscus)
Kim, Sangsik ; Choi, Seung Hwan ; Yoon, Sung Jin ; Hwang, Dong Soo ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 41~46
DOI : 10.15433/ksmb.2014.6.2.041
Mussel byssus is a bundle of threads used to attach mussels to wet substrates. Recently, a thin cuticle layer on the byssus has attracted public attentions due to its remarkable toughness - stiff as epoxy resin and extensible as rubber. Here, we observed ultrastructure of the cuticle layer in a far eastern mussel (Mytilus coruscus) to understand underlying mechanisms for the mechanical properties. The cuticle layer observed by TEM was composed of submicron-sized granular inclusions in a continuous matrix phase. In addition, ultrastructural study in the presence of tertiary amine (Tetraethylammonium, TEA) showed an evidence that the cuticle is stabilized by cation-
Investigation of cultivation and FAME composition isolated Phaeodactylum tricornutum from Youngheung island
Lee, SangMin ; Cho, Yonghee ; Shin, ong-Woo ; Jeon, Hyonam ; Ryu, YoungJin ; Lim, Sang-Min ; Lee, Choul-Gyun ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 47~52
DOI : 10.15433/ksmb.2014.6.2.047
Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.
Effect of Antimicrobial Activity of the Glutaraldehyde Cross-linked Glucosamine
Lee, Choon Geun ; Hwang, You Jin ; Park, Jae Kweon ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 53~61
DOI : 10.15433/ksmb.2014.6.2.053
This study was investigated the antimicrobial activity of glutaraldehyde cross-linked glucosamine. Glutaraldehyde was used as a cross-linker which specifically combines an amine-group of molecules. To optimize the mixing ratio of glutaraldehyde and glucosamine, mixing ratio was set up 1:1, 2:1, 3:1 and 0.5:1 in molarity, respectively. The optimum mixing ratio of glucosamine and glutaraldehyde was found to be 3:1 using thin layer chromatography based on the production of complex. Glucosamine-glutaraldehyde cross-linked complex (Ggcc) revealed significant antimicrobial activity toward PWG than F1, both microbial strains were isolated from porcine semen as antibiotics resistance bacteria (ARB). These results clearly demonstrate that Ggcc has potential bactericidal activity toward ARB in porcine semen.
Development of Bioactive Substances from Fishery Processing by-products in Jeju
Kang, Nalae ; Lee, WonWoo ; Ko, Ju-Young ; Kim, Hyun-Soo ; Kim, Junseong ; Ahn, Yong-Seok ; Ko, Chang-Ik ; Jeong, Joon Bum ; Jeon, You-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 62~67
DOI : 10.15433/ksmb.2014.6.2.062
In this study, we investigated the bioactive substances of the Alcalase hydrolysate obtained from fishery processing by-products in Jeju by measuring bioactivities including radical scavenging acitivty, cytoprotective activity against 2,2-azobis-(2-amidino-propane) dihydrochloride (AAPH), and ACE inhibitory activity. This study is important because of utilization of unused fishery processing by-products in Jeju. The Alcalase hydrolysate was prepared through the hot water extraction and enzymatic hydrolysis, and then further separation of the Alcalase hydrolysate was performed by ultrafiltration using 10 kDa molecular weight cut-off membrane. The Alcalase hydrolysate showed the relatively higher DPPH and peroxyl radical scavenging activity (
value; 1.30 mg/ml and 0.888 mg/ml, respectively). Also, the Alcalase hydrolysate showed the ACE inhibitory activity with 1.87 mg/ml of
value. These biological activities are increased over 1.2 or 2.5 times through the ultrafiltration of the Alcalase hydrolysate. Therefore, the Alcalase hydrolysate obtained from fishery processing by-products in Jeju and the different molecular weight fractions should be given consideration for food and cosmetics ingredient. Furthermore, this research on the utility of fishery processing by-products might be a useful tool into the industry.
Influence of Organic Carbon Sources on Growth and Lipid Content of Marine Green Alga Dunaliella tertiolecta
Rizwan, Muhammad ; Mujtaba, Ghulam ; Lee, Kisay ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 68~75
DOI : 10.15433/ksmb.2014.6.2.068
This study investigated the potential use of various organic carbon sources (glucose, glycerol and acetate) and different concentrations of
for culturing marine microalga Dunaliella tertiolecta. Cell growth and lipid production were monitored under heterotrophic, mixotrophic and photoautotrophic modes of cultivation. D. tertiolecta showed the ability to grow under mixotrophic (acetate and glucose), heterotrophic (glucose) and photoautotrophic condition under high
concentration (15%). With all the organic carbon sources (glucose, glycerol and acetate) tested in this study, 1~5% acetate enhanced cell growth rate and lipid content, while higher concentrations of acetate (10% and 15%) were inhibitory and resulted in cell death.
Change of Sponge(Axinella sp.)-Associated Bacterial Community during the Cultivation with Hexabromobenzene
Seo, Hyun-Seok ; Yang, Sung-Hyun ; Bae, Seung Seob ; Lee, Jung-Hyun ; Kwon, Kae Kyoung ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 76~83
DOI : 10.15433/ksmb.2014.6.2.076
Bacteria associated with marine sponges seemed to be concerned in halogenation/dehalogenation process of natural compounds. In the present study, the effect of hexabromobenzene (HBB) on the community structure of bacteria associated with a marine sponge Axinella sp. from Chuuk State under anaerobic condition was investigated. Regardless of 100 ppm HBB, most of detected microorganisms displayed high similarity with clones reported from coral or sponges. Amongst, Desulfovibrio marinisediminis like clones were dominant. Clones affiliated with Lentisphaerae and Fusibacter paucivorans (Clostridia) were detected at the conditions without HBB but clones affiliated with Vallitalea guaymasensis (Clostridia) increased its proportion with HBB. From these results and previous reports clones affiliated with D. marinisediminis and V. guaymasensis seemed to be concerned in halogenation/dehalogenation process.
A study on Strategic Planning of Marine Biotechnology for Next Generation
Kang, Gil-Mo ; Jang, Duckhee ; Choi, Yong-Jin ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 84~101
DOI : 10.15433/ksmb.2014.6.2.084
The aim of this project is to develop an adaptive and collective National Marine Biotechnology Plan for the next decade(2014~2023) which is able to reflect current and future changing environment. This effective strategy targets to foster marine-derived active bio-materials, marine bioenergy production technology and many promising technologies in order to promote marine biotechnology industry as a next-generation growth engine. Marine biotechnology industry based on R&D activities since 1980 has been growing as an emerging industry. This new field enables to secure exclusive patent rights and to find new potential bio-active materials from the ocean that requires long-term aggressive R&D investments. The current policy direction is to raise appropriate level of R&D investment because the current Korea's national marine biotechnology R&D fund ratio is less than 2% of the total national biotechnology R&D budget. The result shows three major strategies. First, it recommended a research implementation system and supporting policy that includes establishment of open innovation framework for the 'Industry-Academia-Research Institute Collaborations', strategic research planning and enhanced policy making process. Second, it derived state-of-the-art or new technology in many areas. Third, it formulated more detailed execution plans for successful R&D support and set up performance indicator system in related R&D program.
Molecular adaptation of the CREB-Binding Protein for aquatic living in cetaceans
Jeong, Jae-Yeon ; Chung, Ok Sung ; Ko, Young-Joon ; Lee, Kyeong Won ; Cho, Yun Sung ; Bhak, Jong ; Yim, Hyung-Soon ; Lee, Jung-Hyun ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 102~109
DOI : 10.15433/ksmb.2014.6.2.102
Cetaceans (whales, dolphins, and porpoises) are aquatic mammals that experienced drastic changes during the transition from terrestrial to aquatic environment. Morphological changes include streamlined body, alterations in the face, transformation of the forelimbs into flippers, disappearance of the hindlimbs and the acquisition of flukes on the tail. For a prolonged diving, cetaceans acquired hypoxia-resistance by developing various anatomical and physiological changes. However, molecular mechanisms underlying these adaptations are still limited. CREB-binding protein (CREBBP) is a transcriptional co-activator critical for embryonic development, growth control, metabolic homeostasis and responses to hypoxia. Natural selection analysis of five cetacean CREBBPs compared with those from 15 terrestrial relatives revealed strong purifying selection, supporting the importance of its role in mammals. However, prediction for amino acid changes that elicit functional difference of CREBBP identified three cetacean specific changes localized within a region required for interaction with SRCAP and in proximal regions to KIX domain of CREBBP. Mutations in CREBBP or SRCAP are known to cause craniofacial and skeletal defects in human, and KIX domain of CREBBP serves as a docking site for transcription factors including c-Myb, an essential regulator of haematopoiesis. In these respects, our study provides interesting insights into the functional adaptation of cetacean CREBBP for aquatic lifestyle.
Effects of nitrogen and organic carbon sources on growth and lipid production of Chlorella sp. KR-1 in flask cultures
Lee, Ja-Youn ; Seo, Kyoung Ae ; Oh, You-Kwan ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 110~117
DOI : 10.15433/ksmb.2014.6.2.110
Recently microalgae have been proposed as a promising biodiesel feedstock, owing to their higher lipid productivity and non-arable land based cultivation system. Biomass and lipid productivities of microalgae are largely affected by various environmental and nutritional factors. In this study, the effects of nitrogen (nitrate and ammonium) and organic carbon (glucose and glycerol) sources on the cell growth and lipid production of Chlorella sp. KR-1 were examined in flask cultures. Under autotrophic culture conditions for 15 days, overall cell growth and lipid (fatty acid methyl ester, FAME) production with nitrate were better than those of ammonium, resulting in 1.06 g cell/L and 333 mg FAME/L, respectively. Maximal intracellular lipid contents (348 - 352 mg FAME/g cell) were observed at low concentrations of 1 mM for both nitrate and ammonium. In the supply of light, addition of glucose in the range of 1 - 20 g/L showed higher cell densities than the autotrophic cell growth condition. Higher lipid accumulation of 375 mg FAME/g cell could achieved at 5 g glucose/L albeit of relatively short incubation of 7 days. With glycerol, intracellular lipid contents were ~1.9 times lower than glucose cases although similar cell growths were observed for both carbon sources.
Investigation of Microalgal Growth, Tetraselmis sp. KCTC12432BP by Supplying Bicarbonate on the Ocean Cultivation
Cho, Yonghee ; Shin, Dong-Woo ; Lee, Sangmin ; Jeon, Hyonam ; Ryu, Young-Jin ; Lee, Jong-Chan ; Lim, Sang-Min ; Lee, Choul-Gyun ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 118~122
DOI : 10.15433/ksmb.2014.6.2.118
The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of
concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of
, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time,
concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L
, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the
feeding rate as expected. This research shows that the microalgae can grow with
as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.
Growth of flounder larvae, Paralichthys olivaceus using enriched rotifer fed with artificial microparticle diets
Cho, Kyung-Jin ; Kim, Mi-Ryung ; Park, Heum-Gi ; Lim, Young Soo ; Ra, Chae Hun ; Kim, Sung-Koo ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 123~130
DOI : 10.15433/ksmb.2014.6.2.123
Three types of artificial microparticle diets were developed for rotifer (Brachionus plicatilis) enrichment. The efficacies of enrichment with the artificial diets were evaluated and compared to those with commercial enrichment diets on the growth and survival of flounder larvae. Total lipid content was highest in the rotifer enriched with oil capsule (40.5% in dry weight). The n-3 highly unsaturated fatty acid (n-3 HUFA) content was also highest in the rotifer fed with oil capsule (7.08% in dry weight). The flounder larvae fed on the rotifer enriched with oil capsule showed the highest growth compared to those fed on any other enriched rotifer (P<0.05). The survival ratio of flounder larvae fed on the rotifers enriched with oil capsule and emulsion oil were higher than those fed on any other enriched rotifer (P<0.05). From the feeding study, the growth and survival of flounder larvae were enhanced by feeding rotifer enriched with oil capsule compared to rotifer enriched with any other diets. The rotifer fed on oil capsule containing high contents of n-3 HUFA. Therefore, a significant relationship between the growth and survival of flounder larvae and the n-3 HUFA content of rotifer could be obtained.
Screening of Freshwater Microalgae for Resistance to Ultraviolet Radiation
Han, Mi-Ae ; Han, Hye Jin ; Jung, Moon Hee ; Yoo, Rim Hwan ; Hwang, Chae Eun ; Myung, Su Hyun ; Son, Yun Jin ; Yoon, Young-Sil ; Lee, Choul-Gyun ;
Journal of Marine Bioscience and Biotechnology, volume 6, issue 2, 2014, Pages 131~137
DOI : 10.15433/ksmb.2014.6.2.131
Ultraviolet-B radiation (UV-B) in sunlight causes biological damages such as erythema and blister on skin. Microalgae have been in the limelight as an attractive feedstock for manufacturing functional materials. This study focused on screening microalga with protection ability against UV-B. The microalgae were isolated from local areas on April to June 2013 as well as June 2014. The cells were grown under continuous illumination from fluorescent lamps at
in BG-11 medium at
for 12-14 days. The selected cells were spread on BG-11 agar and were exposed to UV-B (312 nm) for 20 and 25 minutes. The 13 strains among selected algae were classified. Among these, 9 strains were Scenedesmus sp. and the remains were Chlorella sp. Based on this study, it seems that Scenedesmus sp. and Chlorella sp. have resistibility against ultraviolet. These results will help to study on UV protection using microalgae.