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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Marine Bioscience and Biotechnology
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Journal DOI :
The Korean Society for Marine Biotechnology
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Volume & Issues
Volume 7, Issue 2 - Dec 2015
Volume 7, Issue 1 - Jun 2015
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Prediction of Hypoxia-inducible Factor Binding Site in Whale Genome and Analysis of Target Genes Regulated by Predicted Sites
Yim, Hyung-Soon ; Lee, Jae-Hak ;
Journal of Marine Bioscience and Biotechnology, volume 7, issue 2, 2015, Pages 35~41
DOI : 10.15433/ksmb.2015.7.2.035
Whales are marine mammals that are fully adapted to aquatic environment. Whales breathe by lungs so they require adaptive system to low oxygen concentration (hypoxia) while deep and prolonged diving. However, the study for the molecular mechanism underlying cetacean adaptation to hypoxia has been limited. Hypoxia-inducible factor (HIF) is the central transcription factor that regulates hypoxia-related gene expression. Here we identified HIF-binding sites in whale genome by phylogenetic footprinting and analyzed HIF-target genes to understand how whales cope with hypoxia. By comparison with the HIF-target genes of terrestrial mammals, it was suggested that whales may retain unique adaptation mechanisms to hypoxia.
Seasonal Differences of Cultivable Bacterial Communities Associated with the Marine Sponge, Petrosia corticata, Collected from Jeju Island
Jeong, Jong-Bin ; Park, Jin-Sook ;
Journal of Marine Bioscience and Biotechnology, volume 7, issue 2, 2015, Pages 42~51
DOI : 10.15433/ksmb.2015.7.2.042
The community structure of cultivable bacteria associated with the marine sponge, Petrosia corticata, collected from Jeju Island in summer (September) of 2012 and winter (January) of 2013, were compared by the PCR-ARDRA method. Bacterial strains were cultured for 4 days at
on Zobell medium and marine agar medium. After PCR amplification of 16S rRNA gene of individual strains, the restriction enzymes MspI and HaeIII were used to make restriction patterns. As a result, 24 ARDRA patterns from the summer sponge and 20 ARDRA patterns from the winter sponge were obtained. The sequencing result of 1-3 selected strains from each pattern showed over 98% similarities with the known sequences from the public database. At the phylum level, the bacterial community structures of both sponges (summer and winter) were identical qualitatively and composed of 4 phyla : Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Alphaproteobacteria accounted for 42.5% of total in summer sponge and 25.2% in winter, decreasing in the winter sample. Gammaproteobacteria accounted for 27.5% of total in summer sponge and 35.2% in winter, increasing in the winter sample. At the genus and species level, summer sponge had more diverse bacterial communities than winter sponge. Actinobacteria, Bacteroidetes, and Firmicutes increased in the winter sample.
Verification of a Relationship between Ultraviolet Radiation and Initial Microalgal Cell Density Using a Floating Marine Photobioreactor
Kim, Z-Hun ; Park, Hanwool ; Jung, Seong-Gyun ; Kim, Su-Kwon ; Kim, Hee-Yun ; Park, Yong Sung ; Hong, Han Ma Roo ; Lee, Choul-Gyun ;
Journal of Marine Bioscience and Biotechnology, volume 7, issue 2, 2015, Pages 52~57
DOI : 10.15433/ksmb.2015.7.2.052
The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off film were placed in an outdoor rectangular tank containing 200 L of water. At the lower ICDs, 0.01 and 0.05 g/L, biomass productivities in the PBRs without UV cut-off film decreased by
compared with those with the film, respectively. In contrast, the presence of UV cut-off film did not have a significant effect on biomass productivities at the higher ICDs, 0.25 and 1.25 g/L. When the differences in biomass productivity made by the UV cut-off film were plotted against the sum of cell projection area per light receiving area of the PBR, the results revealed that the inhibitory effect of UV on biomass productivity can be negligible when the sum of cell projection area is equal to the light receiving area of the PBR. These results show that photoinhibition caused by UV radiation could be eliminated via operating the PBR with a proper ICD.
Bone Formation Effect of the RGD-bioconjugated Mussel Adhesive Proteins Composite Hydroxypropyl Methylcellulose Hydrogel Based Nano Hydroxyapatite and Collagen Membrane in Rabbits
Kim, Dong-Myong ; Kim, Hyun-Cho ; Yeun, Chang-Ho ; Lee, Che-Hyun ; Lee, Un-Yun ; Lim, Hun-Yu ; Chang, Young-An ; Kim, Young-Dae ; Choi, Sung-Ju ; Lee, Chong-Suk ; Cha, Hyung Joon ;
Journal of Marine Bioscience and Biotechnology, volume 7, issue 2, 2015, Pages 58~70
DOI : 10.15433/ksmb.2015.7.2.058
Injectable RGD-bioconjugated Mussel Adhesive Proteins (RGD-MAPs) composite hydroxypropyl methylcellulose (HPMC) hydrogels provide local periodontal tissue for bone filling in periodontal surgery. Previously we developed a novel type of injectable self-supported hydrogel (2 mg/ml of RGD-MAPs/HPMC) based porcine nano hydroxyapatite (MPH) for dental graft, which could good handling property, biodegradation or biocompatibility with the hydrogel disassembly and provided efficient cell adhesion activity and no inflammatory responses. Herein, the aim of this work was to evaluate bone formation following implantation of MPH and collagen membrane in rabbit calvarial defects. Eight male New Zealand rabbits were used and four circular calvarial defects were created on each animal. Defects were filled with different graft materials: 1) collagen membrane, 2) collagen membrane with MPH, 3) collagen membrane with bovine bone hydroxyapatite (BBH), and 4) control. The animals were sacrificed after 2 and 8 weeks of healing periods for histologic analysis. Both sites receiving MPH and BBH showed statistically increased augmented volume and new bone formation (p < 0.05). However, there was no statistical difference in new bone formation between the MPH, BBH and collagen membrane group at all healing periods. Within the limits of this study, collagen membrane with MPH was an effective material for bone formation and space maintaining in rabbit calvarial defects.
Antioxidant and Antibacterial Activity of Water Soluble Polysaccharide from Defatted Botryococcus braunii
Lee, Da Gyung ; Park, Jae Kweon ;
Journal of Marine Bioscience and Biotechnology, volume 7, issue 2, 2015, Pages 71~78
DOI : 10.15433/ksmb.2015.7.2.071
Sulfated polysaccharides from plants or seaweed are well known for antioxidant, anticoagulant activity or other biological activities. The aim of the present study is to optimize the condition for the isolation of water soluble polysaccharides (WSP) and evaluate the biological activity. WSP was isolated from defatted microalgae B. braunii using chlorosulfonic acid or sulfuric acid. Among WSP isolated using sulfuric acid showed the lowest the content of total carbohydrates (g/L), whereas showed the highest antioxidant activity. Also the preliminary structural analysis of WSP was performed by FT-IR spectroscopy analysis.