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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 11, Issue 2 - Dec 1998
Volume 11, Issue 1 - May 1998
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Studies on the Features of Amyloodinium sp. Parasitized in Black Seabream, Acanthopagrus schlegeli
Ji, Bo-Young ; Kim, Ki-Hong ; Park, Soo-Il ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 13~22
To know the features of Amyloodinium sp. parasitized in Black seabream, Acanthopagrus schlegeli, morphology and reproduction type of the parasite were investigated. Infection mode and histopathology of the parasites were also studied. In the developmental and morphological observation, the parasite passed through parasitic and non-parasitic phases with three developmental stages termed trophont, tomont, and dinospore. The trophont, 30-
ovoidal or pyriform, for the vegetative stage had a spherical nucleus with
in diameter, many food vacuoles and starch grains in cytoplasm. The tomont, 80-
spherical, for the reproductive stage resorbed a stalk, secreted a cyst wall and reproduced within it. A dinospore, small 10-
biflagellated, for the infestation stage had a stigma. The parasite was propagated for 15 days by serial passage in Korean rockfish (Sebastes schlegeli) at
. Trophonts began to detach from the fish about 3 days after infection and was completed within 5 days at
. It took from 3 to 5 days to reproduce at the same temperature. Reinfected fish showed that parasites penetrated under the epithelia of gill filament and gill lamellae causing hyperplasia and degeneration at infected area.
Bacterial Diseases in Flounder Farms of Cheju Island
Oh, Sang-Pil ; Kim, Dae-Hwan ; Lee, Jung-Jae ; Lee, Chang-Hoon ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 23~27
This research was carried out to know the trend in annual occurrence of bacterial disease. The diseased fish were collected from 147 flounder farms of Cheju Island from January, 1991 to December, 1997. Two types of diseases, that is "simple infection" and "mixed infection", were recognized. The simple infections were Vibriosis, Edwardsiellosis Streptococcal infection and Columnaris disease. The mixed infections were caused by a pair of pathogens mentioned above. During the whole period of this study, the highest number of annual occurrence of simple infection was 243 (26.8% of the total) in 1997 and the lowest one was 82 (9.1 %) in 1991. Monthly occurrence of simple infection was the highest number at 132 (14.6% of the total) in August and the lowest one was at 38 (4.2%) in January. Monthly occurrence of the mixed infected disease showed common pattern except November and December. The highest number of annual occurrence of the simple infected disease was 437 (48.2% of the total) in Vibriosis and the lowest one was 22 (2.4%) in Columnaris disease. The highest number of annual occurrence of the simple infected disease was 178 (53.1% of the total) in Vibriosis+Columnaris disease and the lowest one was 28 (8.4%) in Edwardsiellosis+Streptococcal infection.
Spatial distribution of Microcotyle sebastis (Monogenea: Microcotyliidae) on Gills of the Cultured Korean Rockfish, Sebastes schlegeli
Kim, Ki-Hong ; Choi, Eun-Seok ; Ji, Bo-Young ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 29~33
Distribution of a monogenean helminth Microcotyle sebastis on the gills of cultured Korean rockfish (Sebastes schlegeli) was investigated with regard to gill arches, sides of gill hemibranches (anterior or posterior), and their sections (dorsal, medial and ventral). M. sebastis has a significant preference for the second and third pair of gills, and shows marked affinity for anterior hemibranches of each gill branch, and medial sections of each gill hemibranch. The results suggest that the larger volume of water flows and surface area of the second and third pair of gills might affect the distribution of M. sebastis, and the concentrated distribution of M. sebastis on the anterior medial section of gills would be related with the increasing chances of mating by niche restriction.
Anaesthetic Effect of MS-222 and Lidocaine on Abalones, Haliotis discus hannai
Choi, Sang-Duk ; Kim, Ho-Jin ; Suh, Hae-Lip ; Suh, Ho-Young ; Yang, Moon-Ho ; Hwang, Sung-Il ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 35~41
We investigated the optimal concentration of lidocaine and MS-222 (tricaine methanesulfonate) for the exfoliation and recovery of abalone, Haliotis discus hannai in different shell lengths, for the purpose of preventing the damage of shell and muscle. However, most anaesthetics applied at present have a strong toxic effect on abalone. MS-222 is the only anaesthetic which is approved for use in food fish by FDA, and lidocaine belongs to a group of anaesthetic which are used in human medicine. These chemicals were evaluated as anaesthetic for different shell size of abalone. The response varied for different shell size groups (shell length 1, 2 and 3 cm). In this study, we suggested the result that the exfoliation and recovery time by lidocaine and MS-222 in shell length 1 cm group were more shorter than in 3 cm group. In shell length 1 cm group, the optimal concentrations of lidocaine and MS-222 for anaesthetic were 200 ppm and 100 ppm, respectively. Lidocaine and MS-222 are preferable to other conventional abalone anaesthetics since these are cheap, safe and convenient to use.
Comparisons of Immunological Characteristics of Iridoviruses Isolated from Cultured Flounder in Korea
Do, Jeong-Wan ; Cha, Seung-Ju ; Kim, Hyun-Ju ; Cho, Wha-Ja ; Mun, Chang-Hoon ; Park, Jeong-Min ; Park, Myoung-Ae ; Sohn, Sang-Gyu ; Bang, Jong-Deuk ; Park, Jeong-Woo ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 43~50
In order to determine whether the tumor-inducing iridoviruses and the mortality-associated iridoviruses from cultured marine fish in Korea belong to same type, we compared the immunological characteristics of these viruses. The electrophoretical pattern of structural proteins of the tumor-inducing was different from that of the mortality-associated iridoviruses. The antigenicity of structural proteins of these viruses were identified by Western blotting using two monoclonal antibodies against tumor-inducing iridovirus. Two monoclonal antibodies recognized a 150 kDa structural protein of tumor-inducing iridoviruses showed. However, the structural proteins of the mortality-associated iridoviruses did not react with these monoclonal antibodies. These results demonstrate that the antigenicity of the structural proteins of tumor-inducing iridovirus is different from that of mortality-associated iridovirus, indicating that these two iridoviruses belong to different types.
Studies on Ichthyophthirius multifiliis Fouquet, 1876 in Freshwater Fishes II. Experimental Infection and Development of I. multifiliis
Ji, Bo-Young ; Kim, Ki-Hong ; Park, Soo-Il ; Kim, Yi-Cheong ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 51~60
Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature:
DO: 7-7.5 ppm; pH:
). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at
. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at
. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at
would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from
on the 1st day PE to
on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.
Rapid Diagnosis of Iridovirus Infection by Polymerase Chain Reaction
Cha, Seung-Ju ; Do, Jeong-Wan ; Kim, Hyun-Ju ; Cho, Wha-Ja ; Mun, Chang-Hoon ; Park, Jeong-Min ; Park, Myoung-Ae ; Kim, Su-Mi ; Sohn, Sang-Gyu ; Bang, Jong-Deuk ; Park, Jeong-Woo ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 61~67
For rapid detection of iridovirus infection, a PCR-based diagnostic method was developed. The genomic DNA from mortality-associated iridovirus was cloned into pUC19 vector. The nucleotide sequences of these clones were compared with sequences of other genes from EMBL/GenBank databank. Based on the nucleotide sequences, PCR primers were prepared and used for PCR. The DNA amplification did not occur from the normal fish cells. In contrast, DNA was amplified from the iridovirus-infected fish cells and purified iridovirus. These results suggest that mortality-associated iridovirus can be detected from virus-infected cells within short time and this PCR-based diagnostic system provides a simple and accurate method for detecting the presence of iridovirus infection.
Infection and Rapid Detection of Perkinsus sp. In Cultured Babyneck Clam, Ruditapes philippinarum from Western Coast of Korea
Choi, Dong-Lim ; Kwon, Jung-No ; Park, Sung-Woo ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 69~76
An apicomplexan parasite, Perkinsus sp. was observed from the cultured baby clams, Ruditapes philippinarum, collected from the coast of Kochang and Taean (South Korea), where it caused seasonal mortality of clams. Several milky-white cysts were observed on the surface of gill and visceral mass of parasitised clams. The trophozoites of parasite had eccentric nucleus and proliferated by schizogony in gill, mantle, hepatopancrease and reproductive tissues, resulting in the formation of granuloma and the intensive infiltration of hemocytes in the tissues. During incubation in FTM, trophozoites increased in size, resulting in prezoosporangia which appeared as round black spheres when colored with Lugols iodine solution. The prevalence of Perkinsus sp. in clams was Kochang, 73.1%; Taean, 94.8% (during 9-mo. survey) and showed size-dependent infection. Hemacolor kit was useful to reduce time for diagnosis of the trophozoite of Perkinsus sp. that has been responsible of massive motalities in the clam.
A Comparative Study of the Detectable Methods of Residual Oxytetracyeline in Muscle of Flounder (Paralichthys olivaceus) with Simplified Screening Test
Jung, Sung-Hee ; Kim, Jin-Woo ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 77~81
By standardized method, Bacillus subtilis BGA, Bacillus cereus var. mycoides ATCC 11778, and Micrococcus luteus ATCC 9341 were seeded on the muller hinton agar (Difco) plate, and pH was adjusted to 6.0, 7.2, and 8.0. Five agar plates, B. subtilis (pH 6.0), B. cereus (pH 6.0), B. subtilis (pH 7.2), B. subtilis (pH 8.0), and M. luteus (pH 8.0), were employed as test plates of modified EEC 4-plate method. Oxytetracycline (OTC) with a diet was orally administered to flounder, Paralichthys olivaceus, at 100 mg/kg once a day. After oral administration, modified EEC 4-plate method by the three screening test using muscle-direct, extraction-disk and direct-disk methods was conducted for 3 fish at 1, 3, 5, 10, 15, 20, 25 and 30 days. Muscle-direct treatment of B. subtilis (pH 6.0) was found to be dubious positive (
) at the 1st day after the administration; thereafter, it was found to be negative to the last day of the experiment. Extraction-disk and direct-disk treatment of B. subtilis (pH 6.0) were found to be negative from the 1st day to the last day after the administration. B. subtilis (pH 7.2), B. subtilis (pH 8.0), and M. luteus (pH 8.0) by the three screening tests, were found to be negative all the way after the administration. On the other hand, B. cereus (pH 6.0) by the three screening tests was clearly found to be positive for the first 15 days after the administration, and then muscle-direct and direct-disk treatment of B. cereus (pH 6.0) were found to be dubious positive at 20th days after the administration. However extraction-disk treatment of B. cereus (pH 6.0) was clearly found to be negative at the same stage; thereafter, the three screening tests of B. cereus (pH 6.0) were found negative to the last of the experiment. These findings showed that to have equal sensitivity to those determination for the residual detection of OTC, and also confirmed that B. cereus was effective test organism for the monitoring of OTC.
Benedenia derzhavini (Trematoda : Monogenea) from Cultured Korean Rockfish, Sebastes schlegeli, in Korea
Kim, Ki-Hong ; Kwon, Se-Ryun ;
Journal of fish pathology, volume 11, issue 1, 1998, Pages 83~86
The benedeniine monogenean Benedenia derzhabini (Layman, 1930) Meserve, 1938 from cultured Korean rockfish, Sebastes schlegeli, is described and reported for the first time in Korea. The parasite was recovered from the gills and inner wall of operculum. B. derzhavini is distinguished from B. seriolae and B. sebastodis by the relative shape and length between the accessory sclerites and the hamuli.