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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 12, Issue 2 - Dec 1999
Volume 12, Issue 1 - May 1999
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In vitro Effect of Aloe on the Respiratory Burst Activity of Olive Flounder (Paralichthys olivaceus) Leucocytes
Kim, Ki-Hong ; Hwang, Yoon-Jung ; Bai, Sung-Chul ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 1~6
The immunostimulating effect of aloe on respiratory burst activity was investigated by measurements of the chemiluminescent responses (CL) of olive flounder (Paralichthys olivaceus) kidney phagocytes in vitro. The phagocytes incubated with 0.5 and
of aloe for 24 hours showed significantly increased respiratory burst activities compared to control. The result of this study suggests that aloe can be used as an immunostimulant for olive flounder culture.
Effect of Dietary Herb Medical Stuff on the Non-specific Immune Response of Nile Tilapia, Oreochromis niloticus
Hwang, Mi-Hye ; Park, Soo-Il ; Kim, Yi-Cheong ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 7~15
In order to investigate the immune response induced by supplementation of herb medical stuff in diet on the nile tilapia. Oreochromis niloticus, experiments were performed with feeding of four different experimental diets supplemented with 2% ginseng. Panax ginseng, 3% Kugija. Lycium chinense, 3% Hasuo, Polygonum multiflorum, 2% Omija, Schizandra chinensis, respectively, for 84 days. The non-specific immune responses changed during the feeding period were investigated at 2, 4, 6, 8, 10, 12 weeks in each group. Average body weight of the nile tilapia with supplemented diets was heavier than control group. The fish fed on diet supplemented with 3% Kugija showed the better growth than the other tested groups. Complement activity such as complete hemolytic activity (
) and bactericidal activity against Escherichia coli tended to be increased by the supplementation of herb medical stuff. The lysozyme activity of serum and adherent phagocyte activity showed higher in the fish fec on diet supplemented with 3% kugija than the other tested groups. In respect to the RPS against experimental Edwardsiella tarda infection, all of the group fed on the herb medical stuff in diet appeared higher response compared with control group. From these results, herb medical stuff (Panax ginseng, Lycium chinense. Polygonum multiflorum, Schizandra chinensis) might be used a additives of diet for the increasing of non-specific immune response or resistance against bacterial fish diseases.
Effect of Levamisole on Immunomodulation of Eels (Anguilla japonica) In Vitro
Choi, Min-Soon ; Park, Kwan-Ha ; Joung, Kyung-Min ; Shim, Hyun-Bin ; Yun, Sung-Ho ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 16~23
The immunomodulatory effects of levamisole (LMS) were evaluated in leucocytes of eels in vitro. Proliferation of lymhocytes treated with T-cell mitogen (Con A or PHA) was markedly inhibited by LMS in a dose dependent manner. B cell mitogen (LPS), in contrast, slightly increased the proliferaion. On the other hand, production of MIF and MAF when treated with Con A was increased in a dose-dependent way. NK cell activities were somewhat increased when LMS was pretreated and this augmentation was due to an increase in binding capacity of effector-target cell, but not due to the target cell lytic activity of effector cells. Phagocytic activity, superoxide anion formation, hydrogen peroxide formation and lysozyme activity of leucocytes were enhanced by LMS in a dose related-manner. These results suggest that LMS might modulate the immmune responses by activation of cytokine production and by augmentation of leukocyte activity but not by increment of immunocompetent cell numbers.
Qualitative Enzyme-Linked Immunosorbent Assay (ELISA) for the Diagnosis of Edwardsiellosis
Kim, Myoung-Sug ; Hwang, Eun-A ; Huh, Min-Do ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 24~31
Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified
of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of
of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were
cells/ml expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.
Acute Toxicity of Ozone on Survival and Physiological Conditions of Olive Flounder, Paralichthys Olivaceus
Kim, Heung-Yun ; Oh, Myung-Joo ; Jung, Sung-Ju ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 32~41
Ozonization for rearing seawater in land-based culture system has recently been utilized for disinfection of pathogenic microorganisms and improvement of water quality. This study was conducted to examine the effects of total residual oxidants (TRO) in ozone-treated seawater on survival, blood parameters, osmolality and oxygen consumption, and gill tissue of the flounder. Paralichthys olivaceus. Experiments were carried out with the starved flounder of 12~19 cm in total length at
. The 48-hr and 96-hr
for the flounder amounts to 26.4 ppb and 22.3 ppb, respectively. With increase of TRO concentration from 24 to 39 ppb, the values of hematocrit, hemoglobin concentration, red blood cell count and osmolality of the flounder with respect to exposure time were significantly elevated, however, the oxygen consumption rates decreased. In the case of the fish exposed to 13 ppb for 96 hrs, blood glucose increased with an elapse of exposure time, while survival rate was 100%. Death apparently resulted by massive destruction of gill lamellar epithelium, severe osmotic imbalance and the lack of oxygen uptake. The results of this experiment indicated that to protect aquaculture organisms, the ozone-treated seawater should not contain any residual oxidants, and that relatively long-term exposure to TRO of low concentration can impact on survival and physiological conditions of the flounder.
Disinfection of Culture Water Supply by Ozonization I. Susceptibility of Some Fish-Pathogenic Bacteria Isolated From Cultured Marine Fish
Oh, Myung-Joo ; Kim, Heung-Yoon ; Cho, Hyun-Soh ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 42~48
The disinfectant effects of total residual oxidants (TROs) produced by ozonization of natural sea water were investigated against fish pathogenic bacteria isolated from flounder and red seabream. The concentration of 0.1 mg TROs/liter was stable for 20 min in filtered natural seawater, and those of 0.3 and 0.5 mg TROs/liter were also stable for more 1 hr. Disinfectant effects of TRO against Edwardsiella tarda, Vibrio sp., Streptococcus sp. and Staphylococcus sp. were observed with a concentration of 0.1 mg/liter for 180 sec, and the treatment killed more than 99.9% of bacterial cells. With TROs of 0.3 to 0.5 mg/ liter, the viable cells of the bacteria were reduced by more than 99.99% in 60 sec.
Detection of Birnavirus from Cultured Marine Fish Using Polymerase Chain Reaction (PCR)
Oh, Myung-Joo ; Jung, Sung-Ju ; Kim, Young-Jin ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 49~55
To detect birnavirus from cultured marine fish, RT-PCR assay was developed. This method was specific for aquatic birnaviruses that include IPNV Sp., IPNV Ab, IPNV VR-299 and MBV Y6. The birnavirus gene was detected (birnavirus positive samples detected 46/50) from clinical samples signed with abdominal distension and overall darkening even though the samples gave negative results in virus isolation (birnavirus isolate with CHSE-214 cell showed 12/50).
Biological and Serological Characteristics of Birnavirus Isolated from Cultured Japanese Flounder in 1999
Oh, Myung-Joo ; Jung, Sung-Ju ; Kim, Hyeung-Rak ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 56~62
Since 1998, mass mortality of the Japanese flounder has widely occurred in the south and west coastal area of Korea. A new serotype birnavirus was isolated during the investigation of the cause of the disease. By the electromicroscopic examination, the isolated virus particles appeared hexagonal and unenveloped with an average diameter 52 to 56 nm. Birnavirus specific fragment was amplified by RT-PCR. High yield of virus (10.3 to 10.8 log
) was produced in CHSE-214. RTG-2 and RTE-2 cells. Typical birnavirus CPE was observed in these cells. On the contrary, the virus CPE was not shown in the FHM and EPC cells. By a cross-neutralization test with IPNV Ab, IPNV Sp, IPNV VR-299 and MBV Y6, the isolated virus was closely related to marine birnavirus (MBV).
Pseudolepidapedon kobayashii (Digenea: Allocreadiidae) from the Olive Flounder (Paralichthys olivaceus) of the Korean Southern Sea
Kim, Ki-Hong ; Kwon, Se-Ryun ; Ahn, Kyoung-Jin ; Huh, Sung-Hoi ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 63~65
Pseudolepidapedon kobayashii collected from the alimentary canal of the olive flounder, Paralichthys olivaceus, which were caught at the Chinhae Bay, was reported for the first time in Korea, and P. olivaceus was recorded as a new host for P. kobayshii. The morphological characteristics of P. kobayashii were described and compared with the other species in the genus.
Detection of RSIV (Red Sea Bream Iridovirus) in the Cultured Marine Fish by the Polymerase Chain Reaction
Oh, Myung-Joo ; Jung, Sung-Ju ; Kim, Young-Jin ;
Journal of fish pathology, volume 12, issue 1, 1999, Pages 66~69
Occurrences of red sea bream iridovirus disease (RSIVD) in cultured marine fishes were investigated. The infection was detected by the polymerase chain reaction (PCR) used to amplify the red sea bream iridovirus (RSIV). The RSIV infection was widely distributed in fish culture farm around the south coastal area of the Korean peninsula.