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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 12, Issue 2 - Dec 1999
Volume 12, Issue 1 - May 1999
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The dietary supplementing effects of Kugija, Lycium chinense, on immune responses of Nile tilapia, Oreochromis niloticus, to Edwardsiella tarda
Kwon, Mun-Gyeong ; Kim, Yi-Cheong ; Sohn, Young-Chan ; Park, Soo-Il ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 73~81
To study the supplementaing effects of kugija, Lycium chinense, in commercial diet on the immune response of nile tilapia, Oreochromis niloticus, nonspecific immune responses were investigated. The activities of complement and lysozyme were higher in sera of the fish fed diet with kugija than control diet. The effects of kugija on vaccination of fish to Edwardsiella tarda were compared in three different vaccination methods after feeding kugija for ten weeks. Intraperitoneal injection and bath administration with formalin killed cells (FKC) and bath with extracellular products (ECP) after injection of FKC showed differences in immune responses of vaccinated fish. Bath administration with ECP after injection of FKC was more stimulated than any other methods in each of nonspecific and specific responses such as the activities of complement and lysozyme, antibody production and rosette forming cells. Moreover, cumulative mortality was significantly lower in the fish vaccinated with combination FKC and ECP after injection challenge with live E. tarda.
Inhibitory Effect of Green Tea Extract on Collagenase Activity and Growth of Fish Pathogenic Bacteria
Park, Sun-Mee ; Park, Soo-Il ; Huh, Min-Do ; Hong, Yong-Ki ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 83~88
Green tea extract has been suggested to possess properties of collagenase inhibition and antimicrobial effect to fish pathogenic bacteria. Most fish pathogenic bacteria showed collagenase activities of 0.08-0.7 unit/ml. Among them, Edwardsiella tarda produced large amount of collagenase in the ST medium at
after 16 hrs. Biosynthesis of the enzyme from E. tarda was decreased to 1/3 by addition of 0.08-0.8 mg/ml of tea extract in the culture medium. The collagenase activity was inhibited almost 100% in the reaction mixture by 0.2 mg/ml of the extract. Then, the minimal inhibition concentration against E. tarda growth appeared as 8 mg/ml of the tea extract in the culture medium.
Effects of heat and ethanol shock on the membrane proteins of Vibrio vulnificus
Heo, Moon-Soo ; Jung, Cho-Rok ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 89~99
New sixteen heat shock proteins (Hsps) and ten ethanol shock proteins were appeared on the analysis with SDS-PAGE when cultivation temperature for the Vibrio vulnifrcus ATCC 27562 strain was shifted-up to
for 20 mins and treated with of 6% ethanol for 10 mins, respectively. Even the induction of thermotolerance in V. vulnificus was coincided with the induction of Hsps if the pre-shock was adjusted to thermal temperature. Outer membrane proteins (OMPs) that were purified from the membrane of cells after heat shock showed more immunodominant pattern to the immunized rabbit anti-V. vulnificus O serum in enzyme-linked immunosorbent assay (ELISA). On the western immunoblot analysis it was confirmed that both 62 kDa IMP and 69 kDa OMP in the Hsps and 48 kDa IMP a major OMP in the ethanol shock proteins were reacted with rabbit anti-V. vulnificus O sera. Agglutination titer of the heat shocked V. vulnificus with rabbit anti-V. vulnificus O serum was higher than that of the untreated bacteria.
Infestation of Colpoda-like ciliate in the yellowtail Seriola quinqueradiata
Ji, Bo-Young ; Lee, Joo-Seok ; Sim, Doo-Saing ; Kim, Yi-Cheong ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 101~105
In a survey of net-caged yellowtail Seriola quinqueradiata in the southern parts of Korean Peninsula, we found infestations of ciliate in the gills of the moribund fish during the abnormally elevated temperature. The ciliate was very similar to genus Colpoda Muller, 1773 on morphological and reproductive characteristics. Histopathological changes revealed that the ciliate invaded gills only, causing extensive destruction of the gill filaments including lamella without any clinical signs found in the other organs and tissues. This is the first record of the parasitic ciliate in cultured yellowtail.
Pathogenicity of the fish nodavirus causing viral nervous necrosis of sevenband grouper, Epinephelus septemfasciatus
Sohn, Sang-Gyu ; Chun, Seh-Kyu ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 107~113
The pathogenicity of the fish nodavirus causing viral nervous necrosis (VNN) of sevenband grouper, Epinephelus septemfasciatus was examined in sevenband grouper and other marine fish by intramuscular injection. Sevenband groupers of 27~104 g in body weight were highly susceptible to the fish nodavirus, but yellowtail (537 g in body weight), red seabream (207 g), rock bream (43 g), flounder (41 g), tiger puffer (27 g) and rockfish (94 g) of the sizes used to this experiment were not. The pathogenicity of the viral agent to the sevenband grouper was high without regard to fish sizes at rearing water temperature over
, but not at
. Therefore, susceptibility of sevenband grouper to the viral agent causing VNN was thought to be water temperature-dependent rather than fish size (age-dependent). Compared to the infectivity of the viral agent to sevenband grouper with artificial infection methods, fish were successfully affected by intramuscular, intraperitoneal, oral, dipping and cohabitation administrations although there were slight differences in mortalities among infection methods. And survival sevenband grouper after infection with the fish nodavirus was resistant to the reinfection for a long time.
Transferable R plasmid of Edwardsiella tarda isolated from diseased flounders, Paralichithys olivaceus
Kim, Eun-Heui ;
Journal of fish pathology, volume 12, issue 2, 1999, Pages 115~121
MIC test of 16 chemotherapeutic agents was performed on 24 isolates of Edwardsiella tarda collected from flounders. They revealed resistance against combinations of ampicillin, amoxicillin, erythromycin, flumequine, doxycycline(DOXY), nalidixic acid, novobiocin, oxolinic acid, oxytetracycline(OTC), thiamphenicol(TP) and sulfonamide. Two strains carried transferable R plasmid encoding Otc Kanamycin Tp and Otc chloramphenicol Doxy Tetracycline Tp, respectively. The R plasmids were not similar each other on the basis of their digestion pattern of restriction endonuclease, suggesting distribution of different transferable R plasmid among E. tarda from flounders.