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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 13, Issue 2 - Dec 2000
Volume 13, Issue 1 - May 2000
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Inhibitory Activity of Marine Bacterium on the Growth of Vibrio anguillarum
Byon, Ju-Yong ; Kim, Eun-Heui ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 1~6
The antibacterial activity of marine bacterium(Pseudomonas aeruginosa JYMB1-3) was assayed against Vibrio anguillarum with the aim of evaluating the possible use for biocontrolling fish disease as probiotic strain. Inhibition test on the solid medium showed that vibrios were especially sensitive to the JYMB1-3. Edwardsiella tarda, Streptococcus sp. and Staphylococcus epidermidis were also sensitive to that strain, however the antibacterial abilities were varied to the pathogens. The vibriostatic activity of antibacterial substance produced from
cells of the strain for 24 hours was equivalent to
Isolation and Characterization of White Spot Syndrome Baculovirus in Cultured Penaeid Shrimp (Penaeus chinensis)
Heo, M.S. ; Sohn, S.G. ; Sim, D.S. ; Kim, J.W. ; Park, M.A. ; Lee, J.S. ; Choi, D.L. ; Jung, S.H. ; Kim, Y.J. ; Oh, M.J. ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 7~13
Beginning in the summer of 1993, a serious mortality among cultured penaeid shrimp occurred in the western sea of Korea. The typical sign of this disease was white spots inside the surface of the carapace. Cytopathic effect (CPE) were not observed by virus in CHSE-214, RTG-2, but not by pH 11. A nonoccluded rod-shaped form virus was observed by electron microscopy in the lymphoid organ. The virion was bacilliform virus and sourrounded by a virion envelope. Its virion protein was found to be similar to hypodermal and hematopoietic necrosis virus (HHNBV) by analysis of virion proteins in SDS-PAGE. The genome of virus is double stranded DNA molecule whose full length was about 114kb. It was similar to penaeus acute viremia (PAV) of Japan.
White Spot Baculovirus Infection of Shrimp Spawner, Inhabitant Organisms and Survival in Seawater
Heo, Moon-Soo ; Sohn, Sang-Gyu ; Kim, Young-Jin ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 15~20
Infection rates with white spot baculovirus(WSBV) in wild-caught shrimp spawners appeared to be 52% in Penaeus chinensis and 20% in Penaeus japonicus when diagnosed by polymerase chain reaction (PCR). Infection rates of WSBV from inhabitant organisms in shrimp farm showed 37% in Upogebia major and 29% in Calliamassa japonica. The viruses maintained their survivorship in seawater at
, but lost it at
Gill Disease of Pseudobagrus fulvidraco Fingerlings by Deficiency of Pantothenic acid
Lee, Kyung-Hee ; Park, Sung-Woo ; Kim, Young-Gill ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 21~29
A new nutritional disease has occurred among the hatchery-reared Korean bullhead fingerlings (Pseudobagrus fulvidraco) in the Chonbuk Province in September 1997. Diseased fish were all dead within 3-7 days, showing sluggish behavior, head up and tail down swimming. Most characteristic clinical signs were anaemia, clubbed and fused gill, skin desquamation. haemorrhage around the mouth and at the base of pectoral fins. Any causative bacteria and parasites were not isolated from the lesions and internal organs of the diseased fish. The hepatosomatic index, red blood cell count, hematocrit, hemoglobin and erythrocytes size of peripheral blood in the diseased fish were remarkably decreased compared with those of normal fish. In the histopathological observations, epithelial hyperplasia of the gill filaments initiated at the base of the gill was pronounced. This symptom was the characteristic appearence of all the diseased fish. A 0.6% saline bath and feeding a pantothenic acid-supplemented diet were conducted to decrease the mortality. Ten days after 0.6% saline bath or 25 days after feeding a pantothenic acid supplemented diet resulted in decreasing in the mortality. Microscopic appereance of the gill from the recovered fish was similar to that of the gill from healthy fish. These results indicate that the disease was caused by deficency of pantothenic acid in their diet and that 0.6% saline bath or supplementation of pantothenic acid in the diet was an effective way to decrease the mortality.
Effects of Consecutive Blood Collecting Stressors on the Plasma Glucose Level and Chemiluminescent Response of Peripheral Blood Phagocytes in Cultured Sea Bass, Lateolabrax japonicus
Kim, Ki-Hong ; Hwang, Yoon-Jung ; Cho, Jae-Bum ; Ahn, Kyoung-Jin ; Kwon, Se-Ryun ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 31~36
The stress of consecutive blood collectings resulted in evident elevation of plasma glucose level and significant lowering of chemiluminescent response of peripheral blood phagocytes in sea bass (Lateolabrax japonicus). Fish responded to the consecutive stressors in cumulative manners. The plasma glucose level in response to consecutive stressors depended on the stressor intervals. When the plasma glucose level of individual fish was compared with the chemiluminescent response, statistically significant (P<0.05) negative correlations existed.
Toxicity of Chloramine-T on the Flounder, Paralichthys olivaceus, and Bactertcidal Activity Against Fish Pathogenic Bacteria and Blue Green Algae, Tetraselmis suecica
Hwang, Eun-A ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 37~44
CT(Sodium N-chloro-para toluenesulfonamide, Chloramine-T) known to be a strong oxidative agent was investigated to use as a candidate of disinfectant in the marine aquaculture industry by the analysis of the bactericidal activity against different aquatic microorganisms. One hundred percent mortality appeared at and above 16 ppm CT in sea water in flounder, the predominant species in the marine aquaculture of Korea, when exposed for 48hr. However, bactericidal activity was appeared to be very effective, and all different species of the fish pathogenic bacteria exposed to CT of less than 2 ppm in sea water were dead within 15 min. It allowed us to confirm that CT could be a very effective disinfectant to protect the spread of fish pathogenic bacteria derived from diseased fish or sea water in marine aquaculture. High concentration(10 ppm) with longer exposing time(24 hrs) was required for the algicidal activity of CT, at which concentration might induce the acute toxicity against fish, however, restricted the expanded use of CT for the elimination of zooplanktons at phytoplanktons in marine farms. Moreover the bactericidal activity of CT inhibited almost completely in the present of more than 10 ppm of organic materials pointed out that quality of the culturing sea water should be considered carefully for the application CT to the aquatic farms as a therapeutic agent.
In vitro Antimicrobial Activity in Combination of Antibacterials Against Fish-pathogenic Bacteria
Jung, Sung-Hee ; Kim, Jin-Woo ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 45~51
Bacterial diseases with mixed infection have recently occurred at land-based flounder farms in Korea. Thus, single antibacterial is not effective for therapy of mixed bacterial diseases of fish because of their different causative bacteria. The purpose of the present study was to obtain basic data for positive usefulness of a combination of antibacterials used for synergism to mixed bacterial diseases of fish. Snergistic interaction in combination of antibacterials was determined by in vitro antimicrobial activity against selected fish-pathogenic bacteria, Vibrio anguillarum, Edwardsiella tarda, Streptococcus sp., Staphylococcus epidermidis, on the basis of Checkerboard assay using fractional inhibitory concentration (FIC) indices. Synergistic interactions were observed in combinations of (oxytetracycline HCL+lincomycin), (tetracycline HCL+florfenicol), (oxytetracycline HCL+florfenicol) against V. anguillarum, (sodium nifurstyrenate+florfenicol), (tetracycline HCL+florfenicol), (sodium nifurstyrenate+oxolinic acid), (oxytetracycline HCL+florfenicol) against E. tarda, (ciprofloxacine+oleandomycin), (oxytetracycline HCL+oleandomycin), (tetracycline HCL+oleandomycin), (oxytetracycline HCL+lincomycin), (oxytetracycline HCL+spiramycin), (oxytetracycline HCL+erythromycin), (doxycycline HCL+oleandomycin), (tetracycline HCL+spiramycin) against Streptococcus sp., and (ciprofloxacine+erythromycin), (florfenicol+erythromycin), (doxycycline HCL+oleandomycin), (ciprofloxacine+oleandomycin) against S. epidermidis.
The Screening of Marine Birnavirus (MABV) Infected in Brood Stocks of Flounder, Paralichthys olivaceus
Oh, Myung-Joo ; Jung, Sung-Ju ; Kim, Young-Jin ; Kim, Hyeung-Rak ; Jung, Tae-Sung ; Yeo, In-Kyu ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 53~59
Presence of marine birnavirus (MABV) was examined against egg and ovarian fluid, and seminal fluid from the brood stocks of flounder, Paralichthys olivaceus collected from 9 different stations around Korean peninsula. The detection rate of MABV in brood stocks flounder was observed to 34% by PCR. The mean virus titer of the PCR positive fish was
/g(ml). By a neutralization test, all of the isolated virus were ascertained to be closely related to marine birnavirus (MABV).
A Simple Method for the Concentration of Fish Pathogenic Virus in Sea Water
Oh, Myung-Joo ; Kim, Suk-Ryul ; Jung, Sung-Ju ; Kim, Hyeung-Rak ; Kim, Heung-Yun ; Yeo, In-Kyu ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 61~66
A method was developed for concentrating fish pathogenic virus from sea water using membrane ultrafiltration system and centricon. The method consists of passing large volumes (Ca. 20 liter) of sea water through ultrafiltration (PAN) filter followed by cross-flow filtration method and centrifugation use the centricon (Plus-20). This procedure permitted the processing of 20 liter of sea water which resulted in a 20,000-fold reduction in the volume of water and greater than 90% recovery of the seeded MABV.
The Distribution of Bacterial Flora in Kunsan bay
Choi, Min-Soon ; Shim, Hyun-Bin ; Joung, Kyung-Min ;
Journal of fish pathology, volume 13, issue 1, 2000, Pages 67~73
This study was carried out to elucidate the preliminary ecosystem of the heterotrophic bacterial flora in Kunsan Bay located in western costal area of Korea. Samples were collected at 5 sampling stations. Among 123 bacterial isolates, 9 genera of bacteria were appeared as follows; Vibrio spp.(44 isolates/35.7%), Pseudomonas spp.(42 isolates/34.1%), Aeromonas spp.(11 isolates/8.9%), Moraxella spp.(9 isolates/7.3%), Enterobacteria spp.(6 isolates/4.8%), Bordetella spp.(3 isolates/2.4%), Alkaligenesis spp.(3 isolates/2.4%), Flavobacterium spp.(2 isolates/1.6%), and Staphylococcus spp.(3 isolates/2.4%) respectively. Total viable heterotrophic bacteria was ranged from
CFU/ml and the most abundant viable counts of bacterial population were showed at the stations 2 and 3. This result indicates that the coastal area around Kunsan bay is getting to contaminate far more by municipal wastewaters and industrial byproducts. and so the outbreak of the bacterial diseases will be increased in fish farms.