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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 23, Issue 3 - Dec 2010
Volume 23, Issue 2 - Aug 2010
Volume 23, Issue 1 - Apr 2010
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Phylogenetic analysis of infectious hematopoietic necrosis virus (IHNV) isolated from cultured rainbow trout Oncorhynchus mykiss in Korea
Kim, Hyoung-Jun ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 1~8
Infectious hematopoietic necrosis virus (IHNV) is the causative agent of IHN, one of the most serious viral diseases of salmonid fish. In this study, glycoprotein (G) gene nucleotide sequence of isolated IHNV RtWanju09 from Jeollabuk-do province was analyzed to evaluate their genetic relatedness to worldwide isolates. As the result, it was revealed that IHNV RtWanju09 isolate belongs to JRt Shizuoka lineage with IHNV RtPy91 and RtJe00. The genetic diversity of G gene between RtWanju09 isolate and RtPy91 isolate from Gangwon-do province was 1.77% and maximum nucleotide diversity among the JRt Shizuoka lineage in Korea was 3.03% during the past 20 years, supporting that the continuous evolution has been occurred among JRt Shizuoka isolates. It was believed that IHNV RtWanju09 isolate has been introduced by the movement of contaminated eggs with IHNV from Gangwon-do to Jeollabuk-do by the reason that the eyed eggs in Jeollabuk-do province used to be obtained from Gangwon-do province. In this study, the domestic transfer of IHNV was firstly investigated by the transfer history of eggs and the phylogenetic analysis using IHNV glycoprotein gene sequence.
Influence of temperature shifts on the kinetics of the specific antibody secreting cells in the olive flounder Paralichthys olivaceus
Yoon, So-Hye ; Hong, Su-Hee ; Jeong, Joon-Bum ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 9~16
We determined the effects of temperature shifts on the kinetics of the numbers of antibody-secreting cell (ASC) in the olive flounder Paralichthys olivaceus immunised with formalin-killed Edwardsiella tarda. When fish that were acclimated to
and immunised at that temperature were transferred to a lower temperature (
) at a various times (immediately, 1, 2 or 4 weeks) after immunisation, both further differentiation of B cells and secretion of antibody from the ASC developed at
were suppressed at
. However, in the converse experiment (
), the magnitude of the humoral immune response was recovered independent of the time of the transfer after immunisation at low temperature, even though the peak levels of each transferred group did not reach the level found in
control group. The results were confirmed by counting the number of specific antibody secreting cells (SASC) in the spleen. This study provides the evidences of the immune reaction that the potential for antibody production in B cells of flounder, the most important species in aquatic industry of Korea, immunized at high temperature is suppressed by subsequent exposure to low temperature and that low temperature-induced humoral immuno suppression can be reversed by exposure to a higher temperature.
Presence of low level infected iridovirus in sea perch Lateolabrax sp. imported to Korea
Jeong, Hyun-Do ; Jeong, Joon-Bum ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 17~25
We have identified an iridovirus CH-1 from sea perch Lateolabrax sp. healthy externally and imported from China to Korea. In a comparison of the nucleotide sequences of the five different genomic regions, the CH-1 appears to be closely related to the ISKNV, IVS-1 and Ehime-1 strains detected in China, Korea and Japan respectively. In quantitative comparison of the viral DNA, level of CH-1 in tissue of imported fish was 10,000 times lower than that of IVS-1 strain presented in the infected rock bream Oplegnathus fasciatus of moribund stage. It allowed us to speculate the possibility of the asymtomatic iridovirus infection in the culturing sea perch. Such possibility of asymptomatic infection was supported by result of no appearance of dead fish with typical symptoms of iridoviral disease in keeping experiment of the imported sea pearch in laboratory for more than three weeks. Such asymptomatic infections with iridovirus were also found in spleen of the culturing and externally healthy sea perch of Korea by the presence of the iridoviral DNA in nested PCR.
Monitoring of pathogens on the cultured Korean rockfish Sebastes schlegeli in the marine cages farms of south sea area from 2006 to 2008
Choi, Hye-Sung ; Jee, Bo-Young ; Cho, Mi-Young ; Park, Myoung-Ae ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 27~35
Pathogens on the cultured 579 rockfish, Sebastes schlegeli in the marine cage farms from Geoje and Tongyeong of the Southern sea were investigated from 2006 to 2008. The pathogens were detected throughout the year at 46.0~90.0% for 3 years and the detection rate was low with an average 58.1% in May and high with an average 81.5% in October. Bacteria only, bacteria-parasite mix and virus only were found in October and November as well as parasite only, whereas infection of parasite only was dominant in May when the temperature increased and in August when the temperature peaked. Of rockfish, Microcotyle sp. and Caligus sp. were dominant for parasitic disease, and Vibrio sp. and Streptococcus sp. were dominant bacteria. For virus, RSIV and VNNV were detected as dominant organisms. While no virus was detected in 2006, VNNV, VHSV and RSIV were detected in 2007 due to
higher temperature than 2006 in the summer season. For total prevalence by rockfish sizes, the highest was found at 50.0~87.1% in 11~15cm sizes and 50% was found in 30 cm size. Parasite showed a similar trend of 50.0~79.6% as the total prevalence. Prevalence for bacteria varied from 1.6% (for 10 cm group) to 23.8% (for 26 cm group) and higher virus prevalence of 21.5% was found from below 25 cm group.
Antimicrobial resistance of Edwardsiella tarda, Vibrio spp., and Streptococcus spp. isolated from olive flounder Paralichthys olivaceus
Kim, Myoung-Sug ; Seo, Jung-Soo ; Park, Myoung-Ae ; Cho, Ji-Young ; Hwang, Jee-Youn ; Kwon, Mun-Gyeong ; Jung, Sung-Hee ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 37~45
In this study, we carried out research on the level of single and multi-drug resistance of bacteria isolated from cultured flounder Paralichthys olivaceus. One hundred sixty one bacteria were isolated from cultured olive flounder Paralichthys olivaceus in Korea and the isolates consisted of Edwardsiella tarda (n=32), Vibrio ichthyoenteri (n=37), Vibrio spp. (n=54), Streptococcus parauberis (n=28) and Streptococcus spp. (n=10). These E. tarda isolates were highly resistant in the order of tetracycline (84.4%) and oxolinic acid (71.9%). V. ichthyoenteri and Vibrio spp. showed resistance ampicillin (94.6% and 81.5%) and tetracycline (56.8% and 42.6%). S. parauberis isolates were resistant ampicillin (57.1%), tetracycline (57.1%) and erythromycin (35.7%). Of the isolates, 84.4% of E. tarda, 73.0% of V. ichthyoenteri, 57.4% of Vibrio spp., 42.8% of S. parauberis and 70.0% of Streptococcus spp. isolates exhibited multi-drug resistance against more than two antibiotics.
Effects of dietary seleno-yeast levels on histological responses in juvenile olive flounder Paralichthys olivaceus
Bai, Sung-Chul ; Kim, Young-Chul ; Lee, Jun-Ho ; Kim, Jae-Won ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 47~55
This study was conducted to investigate the effects of dietary seleno-yeast on histological response in juvenile olive flounder Paralichthys olivaceus. Fish averaging
SD) were fed one of the four semi-purified diets containing 0.56, 1.07, 2.86 and 4.56 mg Selenoyeast/kg diet (
, respectively) in triplicates for 12 weeks. Swelling of blood cell in glomerulus, the rupture of some epithelial cell in the renal tubules and enlarged macrophage were observed in the
group. The hepatopancreas had hepatic cell, capillary and zymogen in the pancreas and was normal in the
group. Swelling of hepatocyte gradually decreased with increase in selenoyeast supplementation. The gill lamellae showed partial abnormal condition (terminal clubbing) in the
group, but there were little differences between these and other treatments. These results indicated that the optimum dietary supplementation level of selenoyeast in juvenile olive flounder, Paralichthys olivaceus, could be less than 1.07 mg selenoyeast/kg diet based on histological responses of the fish.
Effect of temperature on pharmacokinetics of nalidixic acid, piromidic acid and oxolinic acid in olive flounder Paralichthys olivaceus following oral administration
Jung, Sung-Hee ; Kim, Jin-Woo ; Seo, Jung-Soo ; Choi, Dong-Lim ; Jee, Bo-Young ; Park, Myoung-Ae ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 57~67
Effects of temperature (
) on the pharmacokinetic properties of nalidixic acid (NA), piromidic acid (PA) and oxolinic acid (OA) were studied after oral administration to cultured olive flounder, Paralichthys olivaceus. Serum concentrations of these antimicrobials were determined after oral administration of a single dosage of 60 mg/kg body weight (average 700 g). At
, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 24 h and 30 h post-dose, were 11.55, 3.79 and
, respectively. At
, the peak serum concentrations of NA, PA and OA, which attained at 10 h, 15 h and 30 h post-dose, were 6.36, 1.4 and
, respectively. Better absorption of NA and PA was noted at
. The elimination of NA from serum of olive flounder was considerably faster at
. However, both absorption and elimination of OA were not affected significantly by temperature. The kinetic profile of absorption, distribution and elimination of these antimicrobials in serum were analyzed by fitting to a one- and two compartment model, with WinNonlin program. In the one compartment model for NA, AUC, Tmax and Cmax at
, 10.67 h and
, respectively. The AUC,
, 7.72 h and
, respectively. In the one compartment model for PA, AUC,
, 21.15 h and
, respectively. The AUC,
, 12.89 h and
, respectively. In the two compartment model for OA, AUC,
, 23.95 h and
, respectively. The AUC,
, 28.03 h and
Effects of garlic Allium sativum on the immune responses of olive flounder Paralichthys olivaceus
Lee, Jun-Hee ; Woo, Sung-Ho ; Eom, Yong-Hwan ; Hwang, Bun-Ok ; Kwon, Mun-Gyeong ; Bang, Jong-Deuk ; Park, Soo-Il ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 69~83
This study was aimed to investigate the effects of injection of garlic, Allium sativum, extract and immersion in garlic juice on the nonspecific immunity and the resistance against the artificial infection of Streptococcus iniae and Edwardsiella tarda of the olive flounder, Paralichthys olivaceus. The nonspecific immune mechanisms were assessed in terms of lysozyme activity, nitroblue-tetrazolium (NBT) assay and superoxide dismutase (SOD) activity etc. Relative percent survival (RPS) was assessed by the challenge with S. iniae BS10 or E. tarda KE-1. Almost of the garlic extract injected groups showed the enhanced level of the tested nonspecific immune factors. In the challenge test with S. iniae and E. tarda, RPS of 5% garlic extract pre-injected group was much higher than that of any other tested groups, respectively. Almost of the garlic juice immersion tested groups exhibited strengthened nonspecific immune defence factors, lysozyme activity, the number of lymphocytes and neutrophils, NBT reduction and SOD activity in kidney. In the challenge with S. iniae and E. tarda, RPS in the 0.25 g/L of garlic juice immersed group was much higher than any other tested groups, respectively. The results suggest that the garlic extract and juice would be effective to enhance the nonspecific immunity and protective ability of olive flounder against fish disease such as S. iniae and E. tarda.
Aquatic animal health management in stock enhancement
Seo, Jang-Woo ; Cho, Mi-Young ; Kim, Jin-Woo ; Park, Gyeong-Hyun ; Jee, Bo-Young ; Choi, Dong-Lim ; Park, Myoung-Ae ; Oh, Myung-Joo ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 85~98
Ministry of Agriculture, Fisheries and Food (MAFF) and National Fisheries Research and Development Institute (NFRDI) have inspected the hatchery-reared seeds of 22 marine species and 11 freshwater species for aquatic animal diseases in stock enhancement program in 2009. Results showed that total 12 local selfgovernments have been restocking the sea with cultured juveniles. Gyeongsangnam-do, Jeollanam-do, Jejudo and Chungcheongnam-do have a preference for marine species seeds to freshwater species. On the contrary, freshwater species were released mostly in Gyeonggi-do, Jeollabuk-do and Chungcheongbuk-do. In the marine species group, abalone was the most abundant as 24.5%, and then sea cucumber (15.2%), olive flounder (11.5%), swimming crab (5.6%), black sea bream and rockfish (6.8%), rock bream (5.1%), black rockfish (4.6%) and scorpionfish (4.5%) were followed. Crucian carp was the most abundant as 19.4%, and then eel (17.0%), Korean bullhead (12.3%), melanian snail (12.0%), catfish (8.4%) were followed in the freshwater species group. The total number of inspection cases in this study were 1,080 and disqualification cases were 19 by detection of aquatic animals pathogens such as red sea bream iridovirus (RSIV), koi herpesvirus (KHV) or white spot syndrome virus (WSSV).
Introduction of risk analysis for movement of aquatic animals in Korea
Seo, Jang-Woo ; Park, Myoung-Ae ; Choi, Dong-Lim ; Kim, Jin-Woo ; Cho, Mi-Young ; Park, Kyung-Hyun ; Jeong, Hyun-Do ; Oh, Myung-Joo ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 99~106
Aquatic animal diseases control act which is to assure the prevention of outbreak and spread of aquatic animal diseases in Korea has come into effect since 22 December 2008. In order to prevent outbreak and spread of aquatic animal diseases, risk analysis is newly introduced. The introduction of risk analysis for movement of aquatic animals related to implementation of recommendation of the World Trade Organization (WTO) Agreement on the Application of Sanitary and Phytosanitary Measures and should be conducted in accordance with guidelines of OIE Aquatic Animal Health Code. This report involves gathering and analysing the information of international regulation and situations of risk analysis framework in Korea for movement of aquatic animals.
Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles, and their treatment
Seo, Jang-Woo ; Kim, Wi-Sik ; Han, Kyeong-Ho ; Lee, Sung-Hoon ; Yoo, Dong-Jae ; Kim, Yi-Cheong ; Oh, Myung-Joo ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 107~112
Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles in the coast of Yeosu in spring, 2007 was identified. The preadult and adult stages of Pseudocaligus sp. were observed, which consisted of cephalothorax, genital segment and abdomen. Lunules were present on the anterior margin of the frontal plate. In order to develop the treatment method, we examined the effects of formalin and fresh water on the parasite. The Pseudocaligus sp. were detached from the host fish by bathing at concentrations of 50 to 400 ppm formalin within 60 min, but 57% was exterminated to fresh water for 90 min.
Henneguya sp. infection of cultured snakehead, Channa argus fingerings
Choi, Hee-Jung ; Jee, Bo-Young ; Park, Myoung-Ae ; Lee, Deok-Chan ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 113~118
The present work describes myxozoan parasite found in the skin of cultured snakehead, Channa argus fingerings (total length, 5.5~7.2 cm) from Busan, Korea. Nature spores and plasmodia were found in the skin mucus of infected fishes. In fresh state, the total length of the spore was
. The size of spore body was
. The polar capsules were pyriform and the size was
. This is the first report of Henneguya sp. from cultured fish species in Korea, and further studies are necessary for definitive identification.
Peritricha ciliate infection of cultured snakehead, Channa argus
Lee, Deok-Chan ; Choi, Hee-Jung ; Park, Myoung-Ae ; Jee, Bo-Young ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 119~122
Mortality and gill lesions in snakehead, Channa argus (body length range, 20.3-22.2 cm) reared in a aqua-farm in Busan, Korea, were associated with a dense bloom of Apiosoma-like ciliate, Order Petrichida. The size of parasite was
. Histological examination revealed that a severe edema and collapse of the gill tissue were observed in a number of samples of snakehead. The mechanism of gill damage was likely due to physical irritation by the parasite. It is believed that this is the first report of Apiosoma-like ciliate in cultured fish in Korea.
Prevalence of Anisakid larvae in chum salmon Oncorhynchus keta in Korea
Seo, Jung-Soo ; Jun, Eun-Ji ; Jung, Sung-Hee ; Kim, Myoung-Sug ; Park, Myoung-Ae ; Lee, Chul-Ho ; Han, Myoung-Chul ; Kim, Jin-Woo ; Jee, Bo-Young ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 123~129
The infestation status of anisakid type larvae was investigated in migrating chum salmon (Oncorhynchus keta), with different condition (captured area, sex, body portion) during 2006~2008. The mean infection number of anisakid larvae per individual female and male fish captured from Namdae river was
, respectively. The mean infection number of anisakid larvae per individual female and male fish captured from the coastal area of Yangyang was
, respectively. The anisakid larvae were mainly found in abdominal muscles (85%) but only a little in the visceral portion. Two types of anisakid larvae (A. simplex, Contracaecum type) were identified but other anisakid larvae were not detected. To investigate the effect of storing temperature on the viability of anisakid larvae, the section of abdominal muscle were stored at different temperature (room temperature,
). As a result, it was necessary to store at
for more than 6 hrs to kill the larvae. The present results revealed that chum salmon caught in Korea are heavily infected with anisakid larvae, mainly in the abdominal muscle, and A. simplex was dominantly found in this study.
Histopathology of unidentified viral disease of cultured carp Cyprinus carpio
Seo, Jang-Woo ; Kim, Wi-Sik ; Kim, Jong-Oh ; Jung, Sung-Ju ; Kim, Seok-Ryel ; Park, Myoung-Ae ; Oh, Myung-Joo ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 131~135
Unidentified viral disease with high mortalities has been recorded consistently since 1998 in cultured carp Cyprinus carpio in Korea. In the present study, we investigated the histopathological characteristics of the diseased carp to evaluate the relatedness to koi herpesvirus disease (KHVD). Histopathogical examination revealed severe necrotic changes and vacuous cells in the kidney, spleen, liver, pancreas, heart and intestine, but eosinophilic intranuclear inclusion, a typical characteristic of KHV infection was not detected. These results suggest that KHVD may be not a cause for the high mortality occurring among cultured carp in Korea and possibly other unknown viral disease could are related.
Anti-fish pathogenic efficacy of hot water extracts obtained from 5 herbs in-vitro, and efficacy and toxicity in flounder of the one selected herb, skullcap
Lee, Nam-Sil ; Jeong, Sung-Hee ; Jee, Bo-Young ;
Journal of fish pathology, volume 23, issue 1, 2010, Pages 137~143
Hot water extracts of 5 herbs were tested in-vitro for anti-bacterial, anti-fungal, anti-parasitical effects for possible use against fish diseases. Skullcap, Scutellaria baicalensis, was the most effective herb in these 5. The effects of skullcap in cultured flounder were examined for various physiological responses and bacterial disease-prevention follow as feeding skullcap absorbed diet for 4, 8, 12weeks. There were not any significant effects in physiological responses, except beneficial action of growth promotion. No definitive preventive activity was observed with experimental feeding of the extract against infected flounder. As we could not confirm in-vivo antibacterial effects of skullcap in flounder despite its in-vitro efficacy, further studies are needed to define the in-vivo efficacy.