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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 23, Issue 3 - Dec 2010
Volume 23, Issue 2 - Aug 2010
Volume 23, Issue 1 - Apr 2010
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Effects of long double-stranded RNAs on the resistance of rock bream Oplegnathus fasciatus fingerling against rock bream iridovirus (RBIV) challenge
Kosuke, Zenke ; Kim, Ki-Hong ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 273~280
To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.
Characteristics on the Crytocaryon irritans of Rock bream, Oplegnathus fasciatus in the embankment fish farm
Choi, Hye-Sung ; Bang, Jong-Duk ; Park, Myoung-Ae ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 281~291
Crytocaryon irritans infection of rock bream was investigated in embankment fish farm from July to December 2009. Prevalence variation was 20.0~88.0% that was high in september and was low in July. While no mortality was found in July and August, it occurred after mid september. Environmental conditions during the survey period were water temperature
, dissolved oxygen
and salinity 31.9~33.7 psu, and the water temperature during mortality season was
. External symptom of the rock bream was secretion mucus but swimming showed fine in the early infected period. While, the symptoms in the severe outbreak season were excess of mucus, falling off tail and congestion, bleeding spot the surface and weakened swimming. We observed free living and parasitic stages in the gills and body surface of rock bream. Hepato somatic index (HSI) was
, which was low in the September being mortality season but was high in November. The percentages of hematocrit infected with Crytocaryon irritans were 37.3% and 41.0% in July and August, respectively while they gradually decreased to 32.1% and 24.2% in september and October, respectively. Total cholesterol and Triglyceride values rapidly decreased by October. After mortality, AST and ALT were 7 and 5 folds higher compared to non-mortality season.
Efficacy of formalin bath against gill infections with Pseudodactylogyrus spp. in cultured eel Anguilla japonica
Jung, Sung-Hee ; Jee, Bo-Young ; Kim, Jin-Do ; Seo, Jung-Soo ; Kim, Jin-Woo ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 293~302
Effects of formalin on removal of Pseudodactylogyrus spp. were examined against naturally infected eel, Anguilla japonica (weight 89.9~96g) at a water temperature of
. Prior to experiments for removal of the parasite, the hematological toxicity of formalin bath at 0~500 ppm for 30 min~24 h was assessed by hematocrit values (Ht). Based on the results of Ht, appropriate methods of treatment, concentrations and durations, were examined in the main study. There was no significant (P>0.05) change of Ht in 100 to 200 ppm for 24 h. In contrast, Ht increased significantly (P<0.05) at above 300 ppm. This suggests that physiological damage was caused by formalin bath treating with 300 to 500 ppm. Formalin bath with 100 and 200 ppm for 24 h caused significant decreases (P<0.05) in the infection of the parasite. In conclusion, the 100 ppm formalin for 24 hour-bath was found most recommendable for the effective treatment of Pseudodactylogyrus spp. for the gills of the infected eel because of the median lethal concentration (
) of formalin to eel; cumulative mortalities were found to be 0 and 13.3%, respectively, following 24 h bathing.
Effect of the hatchery larval sieving on the larval growth, scuticociliate occurrence, and ensuing spat growth of Patinopecten yessoensis
Jo, Q-Tae ; Kim, Su-Kyoung ; Lee, Chae-Sung ; Lee, Jin-Ho ; Park, Mi-Seon ; Moon, Tae-Suk ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 303~311
Our previous finding summarizing that larval sieving process is inevitable but triggers the outbreak of scutica-like ciliates (SLCs) in the seed production of Patinopecten yessoensis urged further study to determine best suggestable sieving interval in an agreeable range of water quality. In the mass seed production of the scallop, SLC outbreak was closely related to the larval sieving in which larvae were drained on the basis of every 3-day (5T), 5-day (3T), 7-day (2T), or 9-day (1T) from culture tanks onto a mesh screen and placed back into new water in cleaned tanks. The larval performance of growth and survival was clearly dependent on the sieving intervals. It was in order of 3T, 5T, 2T, and 1T for both of growth and survival and in reverse order for SLC infection frequency, confirming that larval sieving is necessary but damageable if it overwhelms the larval resistance. Interestingly, the larval damages by the sieving persisted to their ensuing spat life in terms of nursery growth, survival, and abnormality.
Development of DNA probe for a protistan parasite of tunicate Halocynthia roretzi
Choi, Dong-Lim ; Hwang, Jee-Youn ; Choi, Hee-Jung ; Hur, Young-Baek ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 313~322
Edible tunicate Halocynthia roretzi, one of the most commercially important aquatic organisms in Korea, has been killed by tunic softness syndrome since last decade. The intracellular protistan parasite observed by the transmission electron microscope in hemocytes of the tunicate was considered to be the causative agent of the mass mortality. The goal of the present work is to examine the characteristic features of the parasite by identifying the 18S rDNA sequences of the parasite. The experiments conducted include amplification of presumptive 18S rDNA from diseased tunicate tissues with UNonMet-PCR and sequencing the product. A preliminary phylogenetic analysis was performed on the presumptive parasite rDNA. A digoxigenin labeled DNA probe was designed on the basis of the sequences of rDNA. Dig-ISH assay was conducted to diagnose the protistan parasite. A PCR using UNonMet-PCR primer generated 595 bp SSU rDNA fragment. Subsequently, PCRs with primer pair expended this sequence to 1542 bp. This is the first partial sequences of SSU rDNA gene to be published on the protistan parasite that has presumed causing the mass mortality of tunicate. Since the Dig-ISH technique demonstrated the presence of infection in hemocytes on the all host tissues, the fragment was confirmed to be the intracellular protistan parasite SSU rDNA. A phylogenetic analysis suggested that the protistan parasite may be a unique eukaryote that is closely related to Apicomplexa.
Molecular identification and expression analysis of bactericidal permeability-increasing protein/ LPS-binding protein (BPI/LBP) from Black rockfish Sebastes schlegeli
Kwon, Mun-Gyeong ; Kim, Ju-Won ; Park, Myoung-Ae ; Hwang, Jee-Youn ; Park, Hyung-Jun ; Baeck, Gun-Wook ; Park, Chan-Il ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 323~334
Bactericidal/permeability-increasing protein (BPI) and lipopolysaccharide-binding protein (LBP) are important components of the mammalian innate defence system against Gram-negative infections. The BPI/LBP cDNA was identified from the black rockfish ConA/PMA or LPS stimulated leukocyte cDNA library. The full-length BR-BPI/LBP cDNA was 2118 bp long and contained an open reading frame (ORF) of 1422 bp that encoded 473 amino-acid residues. The 5' UTR had a length of 57 bp, and the 3' UTR 639 bp. The molecular weight and theoretical isoelectric point (pI) values were calculated 51.4 kDa and 9.72, respectively. Compared with other known BPI or BPI/LBP peptide sequences, the most conserved regions of the black rockfish BPI/LBP peptide were found to be the BPI1 N-terminal, BPI2 C-terminal domains and a LPS binding domain. Phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the BPI/LBP sequence of black rockfish and that of other teleosts. The black rockfish BPI/LBP gene was predominantly expressed in the PBLs, head kidney, trunk kidney and spleen. The expression of the black rockfish BPI/LBP molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 24 h following LPS stimulation, with a peak at 12 h post-stimulation.
Characterization of an isolated reovirus from the paradise fish Macropodus opercularis imported from Southeast Asia
Kim, Wi-Sik ; Kim, Soo-Jin ; Kim, Jeong-Ho ; Jung, Sung-Ju ; Kim, Do-Hyung ; Oh, Myung-Joo ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 335~342
In 2008, mass mortality was observed in paradise fish Macropodus opercularis which was imported from Indonesia. PCR of these fish found positive for megalocytivirus and Mycobacterium sp., while an unidentified virus was culture-isolated using CHSE-214 cells. In the present study, we investigated characterization of the unidentified virus and its pathogenicity to determine whether the virus was the causative agent of the mass mortality of paradise fish. The unidentified virus induced cytopathic effect (CPE) with syncytia in CHSE-214 and other fish cells, BF-2, GF, SSN-1, FSP and FFN. The virus was resistant against treatments with IUdR, chloroform, acidity at pH 3, basicity at pH 11 and high temperature at
for 3h. By electron microscopy, the viral particles were spherical having a double capsid structure with approximately 65 nm in external diameter. Viral genome was composed of at least 10-segmented RNA with sizes ranging from 0.7 kb to 3.6 kb. Based on these characters, this virus can be classified into family Reoviridae. This reovirus did not cause any mortality in an artificial experiment conducted by injecting the virus to paradise fish. This indicates that the reovirus is not only responsible for the mass mortality of paradise fish in 2008.
Dietary Effects of Lactic Acid Bacteria on Growth, Hematological and Immune Responses of Grey Mullet, Mugil cephalus
Min, Eun-Young ; Kim, Young-Sug ; Kang, Ju-Chan ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 343~355
This study was conducted to investigate the dietary effects of lactic acid bacteria (LAB) supplementation on growth, hematological and immune responses of grey mullet, Mugil cephalus. Three replicate groups of grey mullet (body length,
; body weight,
) were fed the experimental diets with 0 (control), 1, 2.5 and 5,0 % of LAB for 3 months. Total body length growth rate was increased in 2.5 % supplementation group compared to control (P<0.05). No differences were observed in hematological parameters (hematocrit, hemoglobin, RBCs) and serum chemistry (calcium, magnesium, total protein, glucose, GOT and GPT). The antioxidant activity of grey mullet fed the 5.0 % LAB diets was significantly higher than that of other groups (P<0.05). Both intracellular superoxide anion production and lysozyme activity of kidney were higher in the 2.5 % LAB diet than in the control (P<0.05).
Pharmacokinetics of amoxicillin trihydrate in cultured eel Anguilla japonica by single oral and intravenous administrations
Jeon, Eun-Ji ; Seo, Jeong-Soo ; Kim, Jin-Do ; Jung, Sung-Hee ; Kim, Myoung-Sug ; Hwang, Jee-Youn ; Park, Myoung-Ae ; Jee, Bo-Young ; Kim, Jin-Woo ; Kim, Yi-Cheong ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 357~367
The pharmacokinetic properties of amoxicillin trihydrate (Amox) were studied after single oral administration and single intravenous injection to cultured eel, Anguilla japonica, respectively (average
). Plasma samples were taken at 3, 5, 10, 15, 24, 30, 48, 96 and 144 h post-dose. The kinetic profile of absorption, distribution and elimination of Amox in plasma were analyzed fitting to a two-compartment model by WinNonlin program. In oral dosage of 40 and 80 mg/kg body weight, the peak plasma concentrations of Amox, which attained at 3~12 h post-dose, were 3.4 and
, respectively. In intravenous injection with 1 mg/kg, the peak plasma concentrations of Amox, which attained at 9 h post-dose, was
. The following parmeters were calculated for a single oral dosage of 40 and 80 mg/kg body weight, respectively: AUC (the area under the concentration-time curve)= 464 and
(time for maximum concentration)= 2.1 and 3.6 h;
(maximum concentration)= 3.04 and
. Following intravenous injection at 1 mg/kg, this parameters were AUC=
. The apparent oral bioavailability at 40 and 80 mg/kg were 1.6 and 1.1%, respectively. Despite using the trihydrate form of amoxicillin, the oral bioavailability was low in eel.
Statistical data on infectious diseases of cultured olive flounder Paralichthys olivaceus from 2005 to 2007
Kim, Jin-Woo ; Cho, Mi-Young ; Park, Gyeong-Hyun ; Won, Kyoung-Mi ; Choi, Hye-Sung ; Kim, Myoung-Sug ; Park, Myoung-Ae ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 369~377
The epidemiological study was performed to survey the prevalence of fish pathogens of cultured olive flounder, Paralichthys olivaceus collected in Pohang, Ulsan.Gijang, Keoje and Wando area of Korea from 2005 to 2007. In this study, the fish pathogens were detected from 1,528 among 2,238 fish samples and annual incidences were 60.6% in 2005, 66.7% in 2006 and 72.3% in 2007, respectively. Seasonal prevalence was 63.5% in February, 67.3% in May, 75.1% in August and 64.2% in November for three years. The detection rates of parasites, bacteria or viruses were 36.7%, 32.8% and 31.4%, respectively. 775 cases (34.6%) among 2,238 fish samples showed mixed infection with a different pathogens. The distribution of specific diseases showed that detection rates of diseases occurring the most frequently during the study period were Trichodina spp., (28.2%), viral nervous necrosis virus (24.3%), Vibrio (11.6%), viral hemorrhagic septicaemia virus (10.5%).
Design and implementation of olive flounder Paralichthys olivaceus disease diagnosis program
Han, Chang-Min ; Jung, Sung-Ju ; Oh, Myung-Joo ; Han, Soon-Hee ; Park, Jeong-Seon ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 379~388
This paper presents a computer program for easy and rapid disease diagnosis of olive flounder diseases. To design the program, standard diagnosis process of the 14 olive flounder diseases was first setup, then implemented four-steps diagnosis program. To run program, first input fundamental information such as water temperature, size of the diseased fish. Then sequentially, three categories of key factors for disease diagnosis which include external clinical signs, internal clinical signs and microscopic observations are selected. When a user selects the observed signs of olive flounder from the listed options, the program provides maximum 5 presumed disease candidates in order. The disease information, treatment and prevention methods are provided by connected web server through internet. The program would support fish doctors and farmers by providing easy and rapid diagnosis of diseased olive flounder.
Effects of diets supplemented with Yuzu Citrus junos Siebold ex Tanaka on disease resistance of olive flounder, Paralichthys olivaceus
Jung, Yo-Han ; Kim, Do-Hyung ; Kim, Heung-Yun ; Shin, Tai-Sun ; Oh, Myung-Joo ; Lee, Jung-Ho ; Kim, Jong-Hyun ; Im, Su-Yeon ; Kim, Eun-Heui ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 389~398
Effects of various concentration of yuzu Citrus junos Siebold ex Tanaka in the diets on growth, blood chemistry and disease resistance of olive flounder were determined. Fifteen hundred fish averaging 200~270g were fed on moist pellet containing yuzu at the concentrations of 0%(control), 2.5%, 5% and 7.5% for 3 months. After feeding trial, weight gain of fish fed 2.5% and 5.0% yuza diet was higher than those of fish fed 0% and 7.5% yuza diet but not significant(P>0.05). No differences in hematological and physiological indices of olive flounder were found among the experimental diets except for total cholesterol. Serum lysozyme activity was not significantly different among fish fed experimental diets but bactericidal activity of fish fed 7.5% yuza diet was significantly different from those of fish fed 0, 2.5 and 5% Yuza diets. Survival rates of fish fed 2.5% yuza diet at experimental infection with Edwardsiella tarda and Streptococcus sp. were higher than those of the other experimental groups but not significant. In conclusion, Dietary inclusion of 2.5~5% yuzu seems to have positive effects for fish health condition and disease resistance.
Histopathological observation of the gill of the crucian carp, Carassius auratus by the leech, Limnotrachelobdella sinensis
Park, Myoung-Ae ; Kim, Seok-Ryel ; Kim, Myoung-Sug ; Kim, Jeong-Ho ; Park, Jung-Jun ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 399~407
On the inner side of each operculum of the crucian carp, Carassius auratus (n=10), the leech, Limnotrachelobdella sinensis of 1-4 individuals were parasitic. The leeches had approximately 41.0 mm in total length and 11 mm in width. These body was composed with anterior sucker, neck, trunk and posterior sucker and average length was 2.3 mm, 7.2 mm, 23.3 mm and 8.7 mm respectively. To both sides of the trunk lateral vesicle of 11 pair existed. When observed by SEM, anterior sucker was hemisphere shape and the mouth where proboscis comes out existed with the its center. Proboscis was connected the esophagus directly. Under light microscopy, bloodsucking gill of C. auratus showed lamella fusion, hypertrophy the epithelial cell of the filament and lamella, increased mucocytes and congested capillaries. On the other hand, necrotic and hydropic degeneration epithelial cell of the lamella, and infiltration of the macrophages from some individuals were suggested the secondary infection with the bacteria or virus after bloodsucking activity of the leech.
Effects of various concentrations of garlic powder and garlic extract in the diets on growth, serum chemistry and immune response of juvenile olive flounder Paralichthys olivaceus
Cho, Sung-Hwoan ; Lee, Sang-Min ; Kwon, Mun-Gyeong ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 409~420
Effects of various concentrations of garlic powder and garlic extract in the diets on growth, serum chemistry and immune response of olive flounder were determined. Thirty-five juvenile fish averaging 5.1 g were randomly distributed into 21 of 180 L flow-through tanks. Seven experimental diets with various concentrations of garlic powder (GP) and garlic extract (GE) were prepared in triplicate: GP-0 without garlic supplementation, GP-0.5, GP-1, GP-2, GP-3 and GP-5 diets containing garlic powder at the concentrations of 0.5, 1, 2, 3 and 5%, respectively at the expense of wheat flour and finally, GE-0.4 diet containing 0.4% garlic extract were prepared. At the end of the 8-week feeding trial, serum chemistry of fish was measured. In addition, twenty fish from each tank were artificially infected with E. tarda for the following 96 h to monitor cumulative mortality. Weight gain of fish fed GP-0 diet was higher than that of fish fed GP-1, GP-2, GP-3 and GP-5 diets. No difference in serum criteria (total protein, glucose, glutamate oxaloacetate transaminase, cholesterol and triglyceride levels) of olive flounder was found among the experimental diets except for glutamate pyruvate transaminase. Lysozyme activity of fish fed GP-0, GP-1, GP-3 and GE-0.4 diets was higher than that of fish fed GP-5 diet. The highest cumulative mortality was 93.3% in fish fed GP-0 diet at 96 h after E. tarda infection, followed by GP-3, GP-1, GP-5, GP-2, GP-0.5 and GE-0.4 diets. In considering these results, dietary inclusion of garlic powder and garlic extract has no distinctive positive effect on improvement in growth, serum chemistry and immune response of olive flounder in this experimental conditions, therefore, its application should be carefully considered.
Molecular identification of Anisakid worm third stage larvae isolated from masou salmon Oncorhynchus masou
Jeon, Chan-Hyeok ; Setyobudi, Eko ; Kim, Jeong-Ho ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 421~427
Anisakid nematodes third stage larvae were isolated from the muscles of masou salmon (Oncorhynchus masou). Fish were purchased from Jumunjin fishery market in Gangneung. Four Anisakid third stage larvae were isolated from 4 fish. Molecular identification of the isolated worms was conducted by PCR-RFLP analysis of ribosomal DNA internal transcribed spacer region and direct sequencing of mitochondrial DNA cox2 gene. As results, all the tested individual worms were identified as Anisakis simplex (sensu stricto). This is the first report of molecular detection of anisakid worms in salmonid fishes in Korea.
Expressed sequence tags analysis of immune-relevant genes in rock bream Oplegnathus fasciatus gill stimulated with LPS
Lee, Jeong-Ho ; Kim, Ju-Won ; Baeck, Gun-Wook ; Park, Chan-Il ;
Journal of fish pathology, volume 23, issue 3, 2010, Pages 429~440
We constructed a rock bream (Oplegnathus fasciatus) gill cDNA library and a total of 1450 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were locally done by BLASTX for amino acid similarity comparisons. Of the 1450 EST clones, 1022 EST clones showed significant homology to previously described genes while 428 ESTs were unidentified, and 259 clones were hypothetical, or unnamed proteins. Encoding 313 different sequences were identified as putative bio-defense genes or genes associated with immune response.