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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 24, Issue 3 - Dec 2011
Volume 24, Issue 2 - Aug 2011
Volume 24, Issue 1 - Apr 2011
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Propagation of lymphocystis disease virus (LCDV) in the FIN cell line originated from olive flounder Paralichthys olivaceus fin
Hossain, Mosharrof ; Kim, Wi-Sik ; Jung, Sung-Ju ; Oh, Myung-Joo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 47~51
DOI : 10.7847/jfp.2011.24.2.047
The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE) formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached
/ml at 4 days post infection and the highest titer was measured
/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.
Quantitative analysis of the clinical signs in marine fish induced by Megalocytivirus infection
Jin, Ji-Woong ; Cho, Hye-Jin ; Kim, Kwang-Il ; Jeong, Joon-Bum ; Park, Gyeong-Hyun ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 53~64
DOI : 10.7847/jfp.2011.24.2.053
In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average
of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other laboratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies (
copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs: cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative expression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.
Detection of Megalocytivirus in shellfish using PCR with various DNA extraction methods
Kim, Jin-Woo ; Cho, Mi-Young ; Jin, Ji-Woong ; Kim, Ki-Hong ; Jeong, Hyun-Do ; Kim, Kwang-Il ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 65~73
DOI : 10.7847/jfp.2011.24.2.065
In analysis of DNA viruses from the contaminated shellfish using PCR, preparation method of template DNA is an important factor to get enough copy number of viruses. In this study, we evaluated the efficiency of PCR template of Megalocytivirus (sT50mg-D) DNA obtained from 50 mg digestive gland homogenate of oyster using commercial method, and compared with that obtained from 5 g of the same tissues (T5g-D) after PEG precipitation procedures of virus. Both templates DNA suspended in the same volume of distilled water showed positive results by primary PCR with 35 cycles, and the presence of Megalocytivirus was confirmed in oysters collected from cultured farms in Korea. Moreover, PCR with sT50mg-D allowed us to discriminate the contaminated oyster individually, that can not be done in PCR with T5g-D prepared from the mixture of three different individual oyster to get 5 g digestive gland homogenate. In quantitative analysis with real time PCR, Megalocytivirus concentrations in 50
templates prepared using 0.5~50 mg of one positive sample were appeared in the range 6.14E+00~1.2E+02/
. We were not able to get positive result using template DNA contained less than 6.14E+00 copies. Consequently, 2-step PCR performed with DNA extracts from oyster homogenate of small amount (sT50mg-D) i) was enough to detect the contaminated Megalocytivirus in shellfish, ii) allowed us to do the analysis for individual shellfish rather than mixture of several shellfish and iii) showed the presence of Megalocytivirus in oyster from Korea.
Analysis of antigenicity of viral hemorrhagic septicemia virus (VHSV) glycoprotein from cultured olive flounder Paralichthys olivaceus
Kim, Su-Mi ; Jee, Bo-Young ; Cho, Mi-Young ; Won, Kyoung-Mi ; Kim, Jin-Woo ; Park, Soo-Il ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 75~84
DOI : 10.7847/jfp.2011.24.2.075
The amino acid sequence of glycoprotein of Korean VHSV isolate (KR'01-1) was analyzed using the DNAStar Protean system. Based on the flexibility, hydrophilicity, antigenic index and surface probability, three regions (Gp1, Gp2 and Gp3) were selected as potential antigenic determinants. Three oligopeptides containing the amino acid sequences of the three regions were synthesized and polyclonal antibodies were raised against them. The activities of the antibodies were analyzed by Western blotting and virus neutralization test. The results showed that antibodies raised against oligopeptides Gp1 and Gp2 neutralized the infectivity of VHSV, suggesting that they can be possible candidates for subunit vaccines against VHS diseases in olive flounder.
Characterization of Edwardsiella tarda specific phage isolated from fish farms on west coast of Korea
Lee, Chan-Heun ; Heo, Yong-Ju ; Baek, Min-Suk ; Lee, Jung-Uen ; Kang, Ja-Young ; Han, Mi-Jung ; Kyoung, Se-Bong ; Choi, Sang-Hoon ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 85~93
DOI : 10.7847/jfp.2011.24.2.085
Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E. tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investigated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to
. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.
Determination of epidemiological tetracycline MIC cut-off value for Vibrio ichthyoenteri
Han, Hyun-Ja ; Kim, Do-Hyung ; Lee, Deok-Chan ; Won, Kyoung-Mi ; Lee, Soon-Jeong ; Cho, Mi-Young ; Jee, Bo-Young ; Kim, Jin-Woo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 95~102
DOI : 10.7847/jfp.2011.24.2.095
Normalized resistance interpretation (NRI) analysis for tetracycline was applied to generate information on the epidemiological cut-off value for Vibrio ichthyoenteri isolated from diseased olive flounder (Paralichthys olivaceus) larvae. Thus, 42 strains of V. ichthyoenteri were used to determine minimum inhibitory concentration (MIC) values of tetracycline using Etest. Also, 11 tetracycline resistance related genes were investigated by PCR method. Most tetracycline-resistant strains harbored both tetB and tetM with a few exceptions. NRI-derived mean and 2 SD above the mean of theoretical normal distributions of susceptible isolates were 0.33 mg/L and 1.66 mg/L, respectively. The epidemiological cut-off value for V. ichthyoenteri from the calculations could be set to S
2 mg/L. Of the 42 strains, 15 were classified as non-wild type (NWT), and MIC values of the NWT strains vary regardless of tetB and tetM detection, suggesting that there may be other mechanisms involved in tetracycline resistance in this Vibrio species.
Characteristics of Vibrio isolated from cultured file fish, Stephanolepis cirrhifer in Korea
Lee, Bo-Young ; Bang, Jong-Deuk ; Cho, Mi-Young ; Kim, Jin-Woo ; Won, Kyoung-Mi ; Cho, Young-Ah ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 103~112
DOI : 10.7847/jfp.2011.24.2.103
An epidemic was occurred in Stephanolepis cirrhifer during acclimation in laboratory tanks (water temperature was about
). Diseased fish showed an unique external sign, large cloudy skin surface. We investigated the cause of the disease, and isolated one pure cultured bacterium, that was identified as Vibrio anguillarum by biochemical analysis and sequence analysis of the l6S rRNA and recA genes. During the outbreak, about 30% of S. cirrhifer showed the large cloudy skin surface, and approximately 10% of the stocked fish died. Fish were effectively cured by treatment with oxolinic acid.
Antimicrobial activity of honeybee venom against fish pathogenic bacteria
Han, Sang-Mi ; Lee, Kyung-Gill ; Park, Kwan-Kyu ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 113~120
DOI : 10.7847/jfp.2011.24.2.113
In this study, we examined antimicrobial activity of the bee venom isolated from honeybee (Apis mellifera L.) against fish pathogenic bacteria, Edwardsiella tarda, Vibrio ichthyoenteri and Streptococcus iniae of cultured olive flounder, Paralichthys olvaceus. The bee venom exhibited significant antimicrobial activity against the both Gram-negative bacteria, E. tarda and V. ichthyoenteri and Gram-positive bacteria, S. iniae. Minimum inhibitory concentration (MIC) and minimun bactericidal concentration (MBC) of the bee venom were 17.6
/ml against E. tarda., and 1.76
/ml against V. ichthyoenteri, respectively. MIC and MBC of the bee venom were 3.49
/ml, respectively against S. iniae. The postantibiotic effect (PAE) of the bee venom was 5 hr, 6 hr, and 7 hr against E. tarda, V ichthyoenteri, and S. iniae, respectively. In addition, its antimicrobial activity was stable under various pH conditions. According to these results, the bee venom showed the excellent antimicrobial activity against the tested pathogenic bacteria.
Detection of fish pathogens in cultured juveniles for stock enhancement in 2010
Cho, Mi-Young ; Park, Su-Young ; Won, Kyoung-Mi ; Han, Hyun-Ja ; Lee, Soon-Jeong ; Cho, Young-A ; Kim, Jin-Woo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 121~129
DOI : 10.7847/jfp.2011.24.2.121
Aquatic animal raised in hatcheries play an important role in supplying seedling to stock enhancement and seed quality, especially, seed health is the key factor for survival in the field after release and for stocking effectiveness. We have inspected the hatchery-reared seeds of 33 marine species and 12 freshwater species for legally designated diseases in stock enhancement program in 2010. Results showed that abalone was the most abundant as 20.0% in the marine species group and then sea cucumber (15.6%), olive flounder (8.4%), rockfish (6.7%), black sea bream (6.3%) and swimming crab (6.1%) were followed. Crucian carp was the most abundant as 19.4%, and then eel (11.8%), Korean bullhead (10.9%), mandarin fish (10.8%), melanian snail (8.4%), catfish (7.7%) were followed in the freshwater species group. The total number of inspection cases for eight pathogens in this study were 2,105 and disqualification cases were 30 by detection of aquatic animals pathogens such as koi herpesvirus (KHV), red sea bream iridovirus (RSIV), white spot syndrome virus (WSSV) or viral haemorrhagic septicemia virus (VHSV).
Effects of Bisphenol A to interspecific hybrids between marine medaka Oryzias dancena and javanese medaka O. javanicus
Kim, Bong-Seok ; Song, Ha-Yeun ; Nam, Yoon-Kwon ; Kim, Dong-Soo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 131~139
DOI : 10.7847/jfp.2011.24.2.131
Bisphenol A (BPA) is mainly used in the production of epoxy resins and polycarbonate plastics, which is a known endocrine disruptor and acutely toxic to aquatic organisms. In this study, estrogenic effect of BPA was investigated on hybrid between Oryzias dancena and O. javanicus (ODJ). ODJ were exposed to BPA of various concentrations (eg. 2.5 mg/L, 5.0 mg/L and 10.0 mg/L) for 56 days. The growth rate, abnormality and the ratio of female and male were observed in test group and control group. As a result, the growth was
mm in total length (TL) in 2.5 mg/L,
mm in 5.0 mg/L,
mm in 10.0 mg/L in test group while it was
mm in TL in control group which was not treated with bisphenol A. The result showed that the growth decreased as the concentration of BPA increased. The abnormality rate was 13.6% in control group, 65.4% in 2.5 mg/L, 81.3% in 5.0 mg/L and 98.1 % in 10.0 mg/L which showed increase in abnormality as an increase of BPA concentration. As a result of analyzing ratio of sex in the test group and control group, 6.0% was examined to be interspecific in controls, 76.9% in 2.5 mg/L and 100.0% in 5.0 mg/L and 10.0 mg/L. In conclusion, these results suggest that BPA has estrogenic effect on ODJ.
Physiological responses of Fucus serratus (Phaeophyceae) to high doses of cadmium exposure
Lee, Soon-Jeong ; Cho, Mi-Young ; Han, Hyun-Ja ; Jee, Bo-Young ; Kim, Jin-Woo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 141~152
DOI : 10.7847/jfp.2011.24.2.141
Growth, oxidative stress and antioxidant capacity of Fucus serratus exposed to high doses of Cd were examined. Two sites in Southwest England (Restronguet Point and Bantham Quay) were selected since they had different histories of metal contamination. 1~10 mg Cd
were treated to Aquil medium for up to 14 days. Similar levels of lipid peroxidation but different values of relative growth rates, cupric ion reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging capacity indicated that F. serratus has population-dependent antioxidant strategies. F. serratus demonstrated cadmium resistance with no visual symptoms for 14 days and the population from the polluted area seemed to have more powerful antioxidant strategies. However Fucus from the conserved area also showed protective anti oxidative mechanism.
The specific antibody response of olive flounder Paralichthys olivaceus to route of antigen administration and change in environmental conditions
Kim, Wi-Sik ; Han, Jong-Seok ; Jang, Min-Seok ; Seo, Han-Gil ; Jung, Sung-Ju ; Park, Jung-Jun ; Kim, Seok-Ryel ; Oh, Myung-Joo ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 153~160
DOI : 10.7847/jfp.2011.24.2.153
The specific antibody response of olive flounder, Paralichthys olivaceus to change in rearing-environmental conditions post immunization with antigen (bovine serum albumin, BSA) and different routes of antigen administration were investigated by enzyme-linked immunosorbent assay (ELISA). To test the effect of routes of antigen administration, flounder were injected intraperioneally or intramuscularlly with 1 mg of BSA. In addition, to test the effect of change in environmental condition post immunization, flounder were injected intraperioneally with 1 mg of antigen, and then were exposed to acute thermal change (the water temperature (WT) was decreased from
within 30 min and maintained at
for 3 h), handling (fish were caught and subsequently held out of water for 1 min) or heavy oil (76 g/200 L for 2 days). Consequently, there was no significant difference between intraperioneal (IP) and intramuscular (IM) injections except at 10 days post-immunization. With these results, it suggests that both 1M and IP injections may be used as route of vaccination. Furthermore, no significant difference was observed in the antibody response among the groups exposed to heavy oil, handling, sudden drop of WT and positive control except at 10 days post-immunization. From these results, it was confirmed that specific antibody response was not affected by the above mentioned rearing-environmental conditions, suggesting that vaccination can be employed at changing rearing-environmental conditions.
Infection of Clinostomum complanatum (Rudolphi, 1814) in spotted barbel gudgeon (Squalidus multimaculatus Hosoya et Jeon)
Park, Myoung-Ae ; Seo, Jung-Soo ; Jung, Sung-Hee ; Choi, Hee-Jung ; Jeon, Eun-Ji ; Jee, Eo-Young ; Lee, Wan-Ok ; Woo, Sung-Ho ; Lee, Eun-Hye ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 161~166
DOI : 10.7847/jfp.2011.24.2.161
The metacercariae of Clinostomum complanatum (Rudolphi, 1814) were isolated in the whole body muscle of wild freshwater fish, spotted barbel gudgeon (Squalidus multimaculatus Rosoya et Jeon) from the local river in Yeongdeok, Gyeongsangbuk-do, Korea. The infected fish with bumpy body surface by "yellow grub" showed erratic swimming behavior and some fish were died. The isolated excysted metacercariae were tongue shaped and 2.85~5.89 mm
0.8~1.99 mm in size. 1~98 metacercariae were isolated in individual fish and mean infection intensity was 25. All examined adult spotted barbel gudgeon sizing 7 cm in body length were infected. This is the first report of disease outbreak and mortality caused by C. complanatum infection in wild freshwater fish in Korea.
Thelohanellus misgurni (Kudo, 1919) infection on the fins of Chinese muddy loach Misgurnus mizolepis
Kwon, Se-Ryun ; Kim, Hyoung-Jun ;
Journal of fish pathology, volume 24, issue 2, 2011, Pages 167~171
DOI : 10.7847/jfp.2011.24.2.167
On September in 2010, myxosporidian parasites were found on the fins of Chinese muddy loach, Misgurnus mizolepis, Several cysts of approximately 2 mm diameter were observed on the fins. Numerous spores were found in the cyst. The total length of the spore was 13.0 to 15.8
, and the width of spore was 6.0 to 7.5
. As the results, the myxosporidians were finally identified as Thelohanellus misgurni (Kudo, 1919) on the basis of the presence of characteristic cysts and size of spores.