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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 24, Issue 3 - Dec 2011
Volume 24, Issue 2 - Aug 2011
Volume 24, Issue 1 - Apr 2011
Selecting the target year
Efficacy of a vaccine against Streptococcus parauberis infection in starry flounder Platichthys stellatus Pallas
Lee, Deok-Chan ; Lee, Jae-Il ; Kim, Do-Hyung ; Cho, Mi-Young ; Kim, Jin-Woo ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 189~195
DOI : 10.7847/jfp.2011.24.3.189
Starry flounder, which are recently increasingly cultured in Korea, are known to highly vulnerable to Streptococcus parauberis infection. Five groups of starry flounder (n=30 for each group) were vaccinated with S. parauberis formalin-killed whole cells by intraperitoneal injection at a final concentration of 0, 0.01, 0.1, 1 and 10 mg
. Specific antibody production of 1 and 10 mg
administered groups significantly increased at four weeks post immunization. All vaccinated groups showed higher survival rates than a control group when five groups of fish were challenged with S. parauberis at a dose of
, respectively. In particular, 0.1 or higher concentrations of formalin killed bacterial cells are able to confer the fish high protection against S. parauberis infection.
Occurrence of bi-flagellated protists in the tunics of ascidians Halocynthia roretzi with tunic-softness syndrome collected from Tongyeong, south coast of Korea
Shin, Yun-Kyung ; Kim, Hyoun-Joong ; Park, Kyung-Il ; Choi, Min-Soon ; Jun, Je-Cheon ; Kim, Eung-Oh ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 197~204
DOI : 10.7847/jfp.2011.24.3.197
The edible ascidian Halocynthia roretzi is a commercially important fisheries resource in Korea. However, for the last several years, outbreaks of mass mortalities of the species have been occurring along the south and east coasts of Korea, where most ascidians are produced. Although it is known that tunic-softness syndrome is associated with these mortality events, the agent causing the syndrome has not yet been confirmed. To determine the agent causing tunic-softness syndrome, healthy and diseased ascidians were collected in March 2011 from Tongyeong, on the south coast of Korea, and were used for biological and pathological investigations. The results showed that diseased ascidians exhibited remarkably reduced body fluid, fatness index, and tunic index compared with healthy specimens. Interestingly, bi-flagellated protozoans were observed specifically in the tissue imprints and tunic cultures of diseased ascidians at an occurrence rate of 97.5%. Histological observation showed that the thickness of the tunics of diseased ascidians was reduced by half, and irregular structure and breakdown of the tunic fiber bundles were observed. In particular, flagellate-like cells were observed in the diseased ascidians. Our study clearly shows that bi-flagellated protists are present only in the softened ascidians, suggesting that the flagellates are partly or entirely associated with soft-tunic syndrome. Accordingly, further investigations to verify the effects of the flagellates found in the present study on soft-tunic syndrome should be conducted.
Histopathological observation of the crucian carp Carassius auratus with infected leech Limnotrachelobdella sinensis by scanning electron microscope
Park, Jung-Jun ; Byoun, Ju-Young ; Kim, Jung-Ho ; Choi, Hye-Sung ; Park, Myoung-Ae ; Kim, Seok-Ryel ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 205~211
DOI : 10.7847/jfp.2011.24.3.205
All of the crucian carp, Carassius auratus and 50% of common carp. Cyprinus carpio examined in this study were infected with the leeches, Limnotrachelobdella sinensis. Especially, the infection of C. carpio with L. sinensis was the first report in Korea. The gill of C. auratus showed increased hydrophic degeneration of epithelial cell in the filament, blood congestion, hyperplasia of epithelial cell in the filament and lamellae. In the SEM observation, gill filament was transformed to the cylinder form by the lamellae fusion. The lamellae surface showed degeneration, fragmentation of microridges. The extracellular cartilaginous matrix of the filaments was exposed by the collapse of epidermal layer. In the 18S rRNA analysis of L. sinensis, the relationships among these groups are not clear and not concord with their morphological classification.
Papillomatosis on the skin of the wild marbled sole Pleuronectes yokohamae
Park, Jung-Jun ; Byoun, Ju-Young ; Choi, Hye-Sung ; Park, Myoung-Ae ; Kwon, Mun-Gyeong ; Kim, Seok-Ryel ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 213~223
DOI : 10.7847/jfp.2011.24.3.213
Among wild marbled sole, Pleuronectes yokohamae caught in west coastal area, Chungnam, tumor-bearing fish were found. Parasites and pathogenic bacteria were not detected and CPE was not observed. Using the light microscope, the epidermal layer of wild P. yokohamae was significantly thickened compared to the normal skin. The lesion was formed papillary folds. Hypertropical epithelial cells revealed karyolysis, marked nucleolus and cloud swelled cytoplasm. In the epidermal layer of the lesion, X-cell that is characterized by oval and small pale nucleus and prominent nucleolus was observed. Dermal layer had newly formed vessels. The size of mucous cell in the papilloma lesion was significantly increased compared to the normal. In this study, no pathogens were found, so future works for finding cause of the papilloma in the P. yokohamae are needed.
Histopathological observation of liver in cultured black rock fish Sebastes schlegeli in low temperature season
Choi, Hye-Sung ; Huh, Min-Do ; Lee, Mu-Kun ; Choi, Hee-Jung ; Park, Myung-Ae ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 225~236
DOI : 10.7847/jfp.2011.24.3.225
This study was aimed to determine the fish physical status according to the gross and histopathological findings of liver in cultured black rock fish, Sebastes schlegeli. All 47 fish submitted had no marked abnormalities in the external findings. 42.55% of fish showed normal liver, 25.53% yellow liver, 25.53% atrophic brown liver, 4.26% yellowish-green liver and 2.13% fatty liver in gross examination. Grossly normal liver showed no remarkable change in lobular structure but many vacuoles were found in hepatic cell. Hepatic cells took normal roundish, polygonal shapes containing spherical nuclei. In group of yellow-brown liver, many brown pigments were seen in hepatic cells, MMCs and brown-colored hyaline droplets within cytoplasm of hepatocytes. Yellowish green pigments were seen in hepatic cells and MMCs of yellow green liver and green colored hyaline droplets within hepatocytes. The dilated central veins are highlighted with atrophy of hepatic cells. Outline of atrophic hepatocyte became ambiguous and nucleus frequently become small and pyknotic. Fatty liver showed prominent vacuolar structures in cells as clear spaces or foamy cytoplasm with degenerative nuclei. From these results, it was strongly suggested that hepatic gross and histological findings could be used as important and critical health parameters of fish prior to progression to substantial manifestation as clinical disease.
Current status of pathogen infection in cultured eel Anguilla japonica between 2000 and 2010
Kim, Wi-Sik ; Ok, Ha-Na ; Kim, Do-Hyung ; Kim, Heung-Yun ; Oh, Myung-Joo ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 237~245
DOI : 10.7847/jfp.2011.24.3.237
Disease survey was conducted to investigate the cause of high mortality in 23 farms of eel, Anguilla japonica, during the period from 2000 to 2010. Seven kinds of fish pathogens were confirmed in the tested fish, which included: Pseudodactylogyrus sp. (infection rate: 65.5%, 19/29 samples), aquabirnavirus (ABV, 62.5%, 15/24 samples), Edwardsiella sp. (44%, 11/25 samples), anguillid herpesvirus 1 (AngHV1, 16.7%, 2/12 samples), Heterosporis sp. (10.3%, 3/29 samples), Vibrio sp. (8%, 2/25 samples), Trichodina sp. (3.4%, 1/29 samples). The rate of single infection was 44.8% (13/29 samples), while 62.1% (18/29 samples) showed mixed type of infection with 2 to 5 different pathogens. The most predominant mixed infection were Pseudodactylogyrus sp.-Edwardsiella sp. (10.3%, 3/29 samples), Pseudodactylogyrus sp.-ABV (10.3%, 3/29 samples), Edwardsiella sp.-ABV (6.9%, 2/29 samples). Infection by the above infectious parasites, bacteria and virus was confirmed in tested eels of juvenile to adult sizes that were cultured at
in almost the examined farms.
Application of automatic dry chemistry analyzer (FUJI DRI-CHEM 3000) used to hematological analysis of cultured freshwater fish in low temperature season
Jung, Sung-Hee ; Seo, Jung-Soo ; Kim, Jin-Do ; Choi, Hye-Sung ; Park, Myoung-Ae ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 247~254
DOI : 10.7847/jfp.2011.24.3.247
The purpose of this study was to obtain reference data of parameters for hematological health diagnosis in cultured freshwater fish and also evaluate application of automatic dry-type chemistry analyzer (FUJI DRI-CHEM 3000) used to those blood tests. A blood profile of total 200 fish for rainbow trout (Onchorhynchus mykiss), israel carp (Cyprinus carpio), tilapia (Oreochromis niloticus) and eel (Anguilla japonica) cultured in Inland Fisheries Research Institute of NFRDI was determined by hemoglobin (Hb) and plasma chemistry tests: total protein (TP), albumin (ALB), alkaline phosphatase (ALP), blood urea nitrogen (BUN), lactate dehydrogenase (LDH), triglyceride (TG), total cholesterol (TCHO), creatinine (CRE), aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose (GLU). The values of ALT, TG, LDH, ALB, TCHO, AST and ALP were outside from the minimum and/or maximum of the established detectable range of the analyzer. ALT and TG were not detectable in the range of 67%~61.5%. LDH, ALB and TCHO were not detectable in the range of 36~17%. AST and ALP were not detectable in the range of 5.5~0.5%. However, the values of BUN, CRE, GLU, Hb and TP were below the detectable limits of the analyzer.
Genetic analysis of norovirueses in Busan
Kim, Kwang-Il ; Jin, Ji-Woong ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 255~268
DOI : 10.7847/jfp.2011.24.3.255
For detection of noroviruses (NVs), we compared various PCR primer sets based on reverse transcription nested PCR (RT-nested PCR) in the water samples from Dong brook in Busan, South Korea. We designed various new primer sets based on the most conserved sequences of the capsid protein gene that react with diverse NVs found in Korea. Designed primer sets (KG1F/KG1R and KG2F/KG2R, named as PNK) for the respective genogroups of NVs, genogroup I and II (GI and GII), were applied to detect NVs in the water samples from Dong brook concentrated with ultracentrifugation. In the application to the water samples, proportion of GI (76.47%) and GII (70.59%) in water samples of Dong brook in RT-nested PCR with the primer sets of this study. However, no significant differences of the proportion of the positive samples were not found between RT-nested PCRs with reported and newly designed primer sets. From the nucleotide sequencing, GI and GII of NVs present in Dong brook were appeared to be the members of 1/2/4/5/9/10 genotypes, and 3/4/5/11/13 genotypes respectively. Appeared genotype 4 of GII known as an one of main genotype found in patients of many Asian countries warned us to consider the risks of norovirus in aquatic environments in southern part of Korea.
Isoform-specific response of two GAPDH paralogs during bacterial challenge and metal exposure in mud loach (Misgurnus mizolepis: Cypriniformes) kidney and spleen
Cho, Young-Sun ; Kim, Dong-Soo ; Nam, Yoon-Kwon ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 269~278
DOI : 10.7847/jfp.2011.24.3.269
Gene expression of two glyceraldehyde-3-phosphate dehydrogenase (GAPDH) paralogs was examined during Edwardsiella tarda challenge and heavy metal exposures in mud loach (Misgurnus mizolepis; Cypriniformes) kidney and spleen. Transcription of the two mud loach GAPDH paralogs (mlGAPDH-1 and mlGAPDH-2) was significantly modulated by these stimulatory challenges in an isoform-dependent manner. Based on the real-time RT-PCR analysis, the mlGAPDH-2 transcripts were more preferentially induced by E. tarda challenge, whereas the mlGAPDH-1 transcripts were proven to show more inducibility in response to heavy metal exposure using Cd, Cu, Mn and Zn at
. Their isoform-specific response patterns were closely in accordance with the TF binding profiles in promoter and intron-1 of the two mlGAPDH isoforms, in which the mlGAPDH-2 has more binding sites for immune-related transcription factors than mlGAPDH-1 while the mlGAPDH-1 possesses exclusively metal responsive elements in its intron. Collectively, the mlGAPDHs are potentially involved in cellular pathways independent of glycolysis and the two GAPDH paralogs might undergo functional diversification or subfunctionalization at least at the transcription level.
Altered expression of mud loach (Misgurnus mizolepis; Cypriniformes) hepcidin mRNA during experimental challenge with non-pathogenic or pathogenic bacterial species
Lee, Sang-Yoon ; Kim, Dong-Soo ; Nam, Yoon-Kwon ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 279~287
DOI : 10.7847/jfp.2011.24.3.279
Transcriptional response patterns of mud loach (Misgurnus mizolepis; Cypriniformes) hepcidin, a potential ortholog to human hamp1, in response to experimental challenges with non-pathogenic and pathogenic bacterial species were analyzed based on the semi-quantitative reverse transcription-PCR assay. Mud loach hepcidin transcripts were much more preferentially induced by pathogenic bacterial species (Edwardsiella tarda and Vibrio anguillarum) causing apparent pathological symptoms than by non-pathogenic species (Escherichia coli and Bacillus thuringiensis) displaying neither clinical signs nor mortality. However in overall, the induced amounts of hepcidin transcripts were positively related with the number of bacterial cells delivered in both pathogenic and non-pathogenic bacterial species. Inducibility of hepcidin transcripts were variable among three tissues examined (liver, kidney and spleen) in which kidney and spleen were more responsive to the bacterial challenge than liver. Time course expression patterns of hepcidin mRNAs after challenge were different between groups challenged with pathogenic and non-pathogenic species, although the overall pattern of hepcidin expression was in accordance with that generally observed in battery genes appeared during early phase of inflammation. Fish challenged with E. coli (non-pathogenic) showed the significant induction of hepcidin transcripts within 24 hr post injection (hpi) but the level was rapidly declined to the basal level either at 48 or 96 hpi. On the other hand, hepcidin transcript levels in E. tarda (pathogenic)-challenged fish were continuously elevated until 48 hpi, then downregulated at 96 hpi, although the level at 96 hpi was still significantly higher than control level observed in non-challenged fish. This expression pattern was consistent in all the three tissues examined. Taken together, our data indicate that hepcidin is tightly in relation with pathological and/or inflammation status during bacterial challenge, consequently providing useful basis to extend knowledge on the host defensive roles of hepcidin under infectious conditions in bony fish.
In vitro eliminative effects three sorts of herbal extracts of against Ichthyophthirius multifiliis
Kim, Jin-Do ; Kim, Yi-Cheong ; Jung, Sung-Hee ; Seo, Jung-Soo ; Park, Sung-Woo ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 289~295
DOI : 10.7847/jfp.2011.24.3.289
The in-vitro eliminative effects of against three types of herbal extract and formalin Ichthyophthirius multifiliis were examined. All parasites were killed within one hour after exposure to the 500 fold dilution of the complex herb extract whereas the 10 fold dilution of the fertilized solution of Salvia plebeia R. Br. killed all parasites within one hour after exposure. The 5,000 fold dilution of the extracts of Ginkgo biloba leaves killed all parasites within one hour after exposure. As a comparative agent, formalin killed all parasites within one hour at 100 ppm. As the results, the extracts of Ginkgo biloba leaves extracts have the most eliminative ability against the parasite. No differences were found among different parasite density in eliminative effects of the three types of herbal extracts and formalin. Also there were no changes in the fish gill tissues after exposure for two hours to the 5,000 fold dilution of the extract of Ginkgo biloba leaves.
Trap identification of the constitutive promoter-like sequences from the bacterial fish pathogen, as exemplified by Edwardsiella tarda
Lee, Sang-Yoon ; Kim, Ki-Hong ; Kim, Dong-Soo ; Nam, Yoon-Kwon ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 297~305
DOI : 10.7847/jfp.2011.24.3.297
A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.
Effects of feeding of dried Euglena rubra on Nile tilapia Oreochromis niloticus
Lee, Jae-Geun ; Lee, Han-Na ; Kim, Young-Dae ; Choi, Sang-Hoon ; Park, Kwan-Ha ;
Journal of fish pathology, volume 24, issue 3, 2011, Pages 307~313
DOI : 10.7847/jfp.2011.24.3.307
To examine the effects of Euglena rubra (E. rubra) feeding on Nile tiapia, Oreochromis niloticus were fed diets containing dried powder of E. rubra at 0.5 or 2% for 4 weeks. Growth, selected hematological and non-specific immune parameters were assessed at 2 and 4 weeks. There were no significant changes in body weigh gain and erythrocyte levels. However a significant and diet E. rubra level-related decrease in leukocyte level was noted. Significant increases were observed in respiratory burst activity (nitroblue tetrazolium reduction) and lysozyme activities following E. rubra feeding. These results indicate that E. rubra could be beneficial to fish, but excess feeding could toxic.