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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 25, Issue 3 - Dec 2012
Volume 25, Issue 2 - Aug 2012
Volume 25, Issue 1 - Apr 2012
Selecting the target year
Genotype distribution of infectious haematopoietic necrosis virus (IHNV) in Korea
Cha, Seung Joo ; Jung, Yo Han ; Lee, Hyun Young ; Jung, Ji Yoon ; Cho, Hee Jung ; Park, Mi Seon ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 143~150
DOI : 10.7847/jfp.2012.25.3.143
Infectious haematopoietic necrosis virus (IHNV) is an important fish pathogen that infects both wild and cultured salmonids. Since the first isolation of IHNV from rainbow trout and masu salmon in 1991, a series of IHN disease outbreak has been reported in Korea. In 2011, we isolated two IHNV isolates from rainbow trout cultured in Korea. The full open-reading frame (ORF) encoding the glycoprotein (G) of them were sequenced and the amino acid sequences were phylogenetically analyzed. Phylogenetic analysis of the G revealed that both IHNV isolates were grouped into an Asian genogroup containing Korean IHNV isolates and Japanese IHNV isolates. However, based on their sequence variation, they were divided into different subgroup. While one isolate was similar to other Korean isolates, the other isolate showed a high level of similarity with Japanese isolates, suggesting the possibility of influx of new IHNV strain into Korea.
Outbreak of Anguillid herpesvirus-1 (AngHV-1) infection in cultured shortfin eel (Anguilla bicolor) in Korea
Park, Sung-Woo ; Jung, Eun-Bin ; Kim, Dong-Wan ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 151~158
DOI : 10.7847/jfp.2012.25.3.151
Diseased eel (Anguilla bicolor) displayed severe hemorrhages in the gills, and congestion and swelling in the liver. During the epizootic, the water temperature was
and the morality rates were about 5%. No parasites were found on the gills and skin. Bacteria were not cultured from any internal organs using TSA or SS agar at
for 48 hrs. Histopathologically, the gills showed epithelial hyperplasia in the base of secondary gill lamellae and hemorrhages in the capillaries. Some cells in the proliferated interlamellar epithelia exhibited marginal hyperchromatosis. And severe vacuolated changes in the parenchymal cells and congestion in the central veins were observed in the liver. The specific amplicon (396 bp) was detected from gills and opercula of affected eel PCR using Anguillid herpesvirus-1 (AngHV-1) -specific primer sets HVAPOLVPSD (5-`GTG TCG GGC TTT GTG GTG C-3`) and HVAPOLOOSN (5`-CAT GCC GGG AGT CTT TTT GAT-3`). Sequencing analysis of the amplicon demonstrated that this gene was 99% homologous to the AngHV-1 sequence deposited in GenBank. This is the first report of AngHV-1 outbreak in the farmed shortfin eels (A. bicolor) in Korea. When diseased fish were maintained for 10 days at water temperatures of
, the cumulative mortalities were 100% and 10%, respectively. Even though the AngHV-1 genome in the gills from the eel kept at
was detected using PCR, the structure of gill filaments was similar with that of normal fish. Increasing the water temperature to
was an effective way to diminish the mortality of AngHV-1 affected eel.
Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus
Kwon, Se Ryun ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 159~164
DOI : 10.7847/jfp.2012.25.3.159
Vibrio vulnificus ghosts (VVG) were generated using a mobilizable vector including a thermosensitive expression cassette by conjugation. The vaccine potential of VVG was investigated in mouse. Mice immunized with VVG showed significantly higher antibody titer than those with formalin-killed V. vulnificus. The present study supports the conceptive usefulness of bacterial ghosts as vaccine candidates.
Morphological characterization of Vibrio alginolyticus specific bacteriophage isolated from fish farms on west coast of Korea
Heo, Yong Ju ; Lee, Chan Heun ; Baek, Min Suk ; Ahn, Hyun Mi ; Hwang, Yo Sep ; Park, Kwan Ha ; Choi, Sang Hoon ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 165~172
DOI : 10.7847/jfp.2012.25.3.165
Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.
Vibrio scophthalmi infection in Japanese eel Anguilla japonica during seawater adaption
Lee, Nam-Sil ; Kim, Dae-Jung ; Lee, Be-Ik ; Kim, Sin Kwon ; Kim, Myung Suk ; Kim, Yi Cheong ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 173~180
DOI : 10.7847/jfp.2012.25.3.173
Vibriosis is one of the most prevalent fish disease belonging to the genus Vibrio. In present study, Vibrio sp. isolated from Japanese eel was confirmed as Vibrio scophthalmi using analysis of the genomic sequence of 16S rRNA. The major signs were hemorrhage of body surface and inner surface of abdomen, severe enteritis and retention of ascitis. Histopathological examination revealed blood cell degenerations in various organs (gills, liver, spleen, kidney, heart, intestine), exfoliate of intestinal epithelium, and congestion and hemorrhage in intestinal lamina propria. This is the first case report on V. scophthalmi infection in Japanese eel Anguilla japonica.
Distribution of MIC value of antibiotics against Edwardsiella tarda isolated from olive flounder (Paralichthys olivaceus)
Kim, Myoung Sug ; Cho, Ji Young ; Seo, Jung Soo ; Jung, Sung Hee ; Choi, Hye Sung ; Park, Myoung Ae ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 181~188
DOI : 10.7847/jfp.2012.25.3.181
The minimum inhibitory concentration (MIC) of eight antibiotics against Edwardsiella tarda 49 strains isolated from olive flounder (Paralichthys olivaceus) was determined by the broth microdilution method. E. tarda showed 38.8% and 61.2% resistance to ampicillin and amoxicillin. Both resistance rates of E. tarda were 4.1% against ciprofloxacin and norfloxacin. The
values of oxytetracycline, amoxicillin and oxolinic acid were
value to ciprofloxacin was
which was lowest among eight antibiotics tested.
Current status of anisakid nematode larvae infection in marine fishes caught from the coastal area of Korea between 2010 and 2012
Kim, Wi-Sik ; Jeon, Chan-Hyeok ; Kim, Jeong-Ho ; Kim, Do-Hyung ; Oh, Myung-Joo ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 189~197
DOI : 10.7847/jfp.2012.25.3.189
A survey was conducted to investigate infection of anisakid nematode larvae in 243 wild marine fishes caught from the southern coastal area of Korea between 2010 and 2012. The samples comprised fishes from 9 orders, 30 families and 50 species. Total infection rate of anisakid nematode larvae was 10.7% (26/243 fish), which comprised from Yeosu, 7.4% (7/95) in 2010 and 22.7% (5/22) in 2011; from Jeju, 8.2% (5/61) in 2011; from Wando, 40.9% (9/22) in 2012. Anisakid nematode larvae were not detected in Tongyoung and Wando samples in 2011. Molecular identification of the 89 worms from 26 fish was conducted by PCR-RFLP and/or sequence analysis of internal transcribed spacer (ITS) region of ribosomal DNA. From the results, 6 kinds of anisakis species were identified: Anisakis pegreffii (infection rate: 53.9%, 48/89 worms), Hysterothylacium aduncum (38.2%, 34/89), H. fabri (3.4%, 3/89), hybird (A. simplex X A. pegreffii) (2.4%, 2/89), A. simplex (1.1%, 1/89) and Raphidascaris lophii (1.1%, 1/89). The rate of single infection was 80.8% (21/26 infected fish), while 19.2% (5/26) showed mixed infection with 2 to 3 different anisakis species.
Effects of dietary lipid sources on apoptotic and immune gene expression in head kidney of olive flounder (Paralichthys olivaceus)
Hur, Deokhwe ; Lee, Sang-Min ; Hong, Suhee ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 199~210
DOI : 10.7847/jfp.2012.25.3.199
It can be hypothesized that dietary fatty acids can modulate immune responses in fish by inducing apoptosis of immune cells since dietary polyunsaturated fatty acid (PUFA) increase apoptosis by oxygen radicals generated by peroxidation. Thus we examined the effects of deferent dietary lipid sources such as squid liver oil (FO), linseed oil (LO) and soybean oil (SO) on oxidation (Cytochrome C oxidase; COS), apoptosis (TNF-
Scinderin like) and immune (IL-
and NKEF) gene expression in the main immune organ (head kidney) in olive flounder (Paralichthys olivaceus) by Q-PCR analysis after feeding diets containing each oil (5%) for 15 weeks. Linseed oil and soybean oil were chosen to compare n-3 or n-6 enriched vegetable oils, respectively. Consequently, COS, TNF-
and Scinderin like gene expression was increased in SO group, indicating the induction of oxidation and apoptosis. Meanwhile, no significant difference was found in immune gene expression. In conclusion vegetable oils containing n-3 PUFA like linseed oil seems to be more suitable lipid source than soybean oil for replacement of fish oil in flounder since n-6 PUFA in SO leads to activation of apoptosis pathways within the cellular damage in head kidney.
Residues of ampicillin in blood of cultured olive flounder by oral, injection and dipping administration
Jung, Sung Hee ; Seo, Jung Soo ; Park, Myoung Ae ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 211~219
DOI : 10.7847/jfp.2012.25.3.211
The residue levels of ampicillin (AM) in cultured olive flounder, Paralichthys olivaceus (average 300g) at
were studied by oral, intramuscular and dipping administration (routes). The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01~10 ppm were ranging from 84.45% to 91.26% for plasma. The limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 mg/kg body weight), intramuscular injection (5, 10 and 20 mg/kg body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-administration. In oral dosage of 10, 20 and 40 mg/kg, it`s peak concentrations were
, respectively at 10 h post-administration, but AM was not measurable at 144, 360 and 360 h post-administration, respectively. In intramuscular injection of 5, 10 and 20 mg/kg, it`s peak concentrations were
, respectively at 5 h post-administration, but AM was not measurable at 216, 264 and 264 h post-administration, respectively. In dipping of 10, 20 and 40 ppm, it`s peak concentrations were
, respectively at 3 h post-administration, but AM was not measurable at 264, 264 and 360 h post-administration, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.
Diseases characteristics of cultured hybrids (red seabream ♀ × black sea bream ♂), Japanese red seabream, red seabream and black seabream in marine net cage
Choi, Hye Sung ; Do, Jeong Wan ; Park, Myoung Ae ; Ahn, Chul Min ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 221~229
DOI : 10.7847/jfp.2012.25.3.221
Hybrid of red seabream (Pagrus major) and Japanese red seabream (Pagrus major) grew rapidly from to August (WT
) while black seabream (Acanthopagrus major) did from September to October (WT
). Survival rate of hybrids (85.5%) was a significantly higher than those of the other fish species (20%). Green liver syndrome were observed in black sea bream and Japanease red seabream but did not in hybrids. Microcotyle tai infected 0~90% in hybrid, 20~100% in red seabream, 10% in black sea bream and 20~100% in Japanese red seabream but no significant difference was found among each fish species.
Monitoring of bacteria and parasites in cultured olive flounder, black rockfish, red sea bream and shrimp during summer period in Korea from 2007 to 2011
Jung, Sung Hee ; Choi, Hye-Sung ; Do, Jeung-Wan ; Kim, Myoung Sug ; Kwon, Mun-Gyeong ; Seo, Jung Soo ; Hwang, Jee Youn ; Kim, Seok-Ryel ; Cho, Yeong-Rok ; Kim, Jin Do ; Park, Myoung Ae ; Jee, Bo-Young ; Cho, Mi Young ; Kim, Jin Woo ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 231~241
DOI : 10.7847/jfp.2012.25.3.231
Diagnostic monitoring in fish farms with land-based tanks and netpen cases were conducted in eastern, western, southern and Jeju island of Korea during summer of 2007~2011. In total, 2413-fish samples of 4 marine fish species were tested for the detection of bacteria and parasite. Fish species tested were olive flounder (Paralichthys olivaceus), black rockfish (Sebastes schlegeli), red sea bream (Pagrus major), pacific white shrimp (Litopenaeus vannamei). During the diagnostic monitoring from 2007 to 2011, the infection rates by single infection of bacterial or parasitic pathogens were relatively higher than the mixed infections. The main bacterial pathogens in olive flounder, black rockfish and pacific white shrimp were Vibrio spp. (V. harveyi, V. ichthyoenteri, Vibrio sp.). The main bacterial pathogens in red sea bream were also Vibrio sp. and Photobacterium damselae subsp. damselae. The main parasitic pathogens were both Miamiensis avidus and Trichodina sp. in olive flounder, Microcotyle sebastes in black rockfish, Microcotyle tai in red sea bream and Zoothamnium sp. in pacific white shrimp.
Histological observations on skin papilloma in wild red halibut (Hippogossoides dubius)
Park, Sung-Woo ; Yu, Jin-Ha ; Jung, Eun-Bin ; Song, Jae-Hee ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 243~247
DOI : 10.7847/jfp.2012.25.3.243
Histopathological observations on a epidermal papilloma of wild red halibut (Hippogossoides dubius) caught at the area around Echung Island and Gogunsan Islands were done. The papilloma formed on the dorsal fin was the same color with the body on the ocular side but black on the non-ocular side. Under light microscopy, the tumor on the skin was very similar to epithelial papilloma supported by connective tissue. X-cell-like cells in the tumors were large and eosinophilic with a centrally-located, large nucleus. But the characteristic large nucleolus in the nucleus of the x-like cells was indistinct clear in this specimen because about 6-9 hours have passed after catching the fish and autolysis have occurred.
Diseases of the cultivated Porphyra at seocheon area
Lee, Soon Jeong ; Park, Sung Woo ; Lee, Jong Hwa ; Kim, Young Sik ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 249~256
DOI : 10.7847/jfp.2012.25.3.249
Disease occurrence and dead cell ratio of Porphyra cultivated at Seocheon area were studied. Seaweed samples were collected monthly at 12 cultivating sites of Porphyra from Nov. 2008 to Mar. 2009. Although disease occurrence was low at the beginning of cultivation at the most of collecting sites, the occurrence increased with time. The most common symptom of Porphyra disease in this area was diatom attachment, followed by white rot disease. Licmophora sp. was the most common species of diatom and its attachment was the highest on November, the beginning of cultivation. Although the occurrence ratios of green spot disease and white rot disease were high on November, chytrid disease was increased at the end of cultivation period. Dead cell ratio of Porphyra thalli was different at each study site and cultivating time, but normally the ratio was high at the end of cultivating season. The changes of dead cell ratio could be related to the nutrition supplement and currents, and the more precise reason of dead cell ratio would be revealed by further research related to cultivating environment conditions.
Evaluation of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of infectious hematopoietic necrosis virus (IHNV)
Kim, Wi-Sik ; Jeon, Chan-Hyeok ; Kim, Jeong-Ho ; Oh, Myung-Joo ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 257~262
DOI : 10.7847/jfp.2012.25.3.257
A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was evaluated to monitor infectious hematopoietic necrosis virus (IHNV) from artificially infected rainbow trout Oncorhynchus mykiss. The cumulative mortalities of fish challenged with IHNV at
/fish were 40%, 0% and 0%, respectively. Dead fish and survivors at 16 and 28 d post-challenge in each group were employed for IHNV detection by RT-LAMP assay and virus isolation using BF-2 cells. IHNV from
was isolated from all the dead fish and also detected in all of the examined dead fish by RT-LAMP assay. In survivors at 16 d, 60% (3/5 fish,
), 20% (1/5 fish,
) and 60% (3/5 fish,
) were found to be IHNV-positive by virus isolation in fish challenged with IHNV at
/fish, respectively, while 20% (1/5 fish), 0% (0/5 fish) and 20% (1/5 fish) were IHNV-positive by RT-LAMP assay. No IHNV was detected in the survivors at 28 d and control fish. These results indicate that the RT-LAMP assay is useful for detection of IHNV in diseased fish although it is not enough to monitor virus in IHNV-survivors.
Comparison of pathogen detection from wild and cultured olive flounder, red sea bream, black sea bream and black rockfish in the coastal area of Korea in 2010
Park, Myoung Ae ; Do, Jeung-Wan ; Kim, Myoung Sug ; Kim, Seok-Ryel ; Kwon, Mun-Gyeong ; Seo, Jung Soo ; Song, Junyoung ; Choi, Hye-Sung ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 263~270
DOI : 10.7847/jfp.2012.25.3.263
This study surveyed for the prevalence of parasites, bacteria and viruses in four fish species, olive flounder (Paralichthys olivaceus), red sea bream (Pagrus major), black sea bream (Acathopagrus schlegeli) and black rockfish (Sebastes schlegeli) in 2010. The survey was aimed to compare the pathogens detected from wild and cultured fish for an epidemiological study. Anisakis sp. was predominantly detected from wild olive flounder and red sea bream (58.6% and 41.7% respectively), but not from the cultured fishes, suggesting anisakid infection is rare in cultured fish. The wild fish get in contact with the anisakids through their prey such as small fishes or crustaceans which carry the anisakids; whereas the cultured fish are fed with formulated feed, free of anisakids. Bacterial detection rates from the wild fishes examined in the study were lower than those of cultured fishes. Vibrio sp. dominated among detected bacterial population in cultured olive flounder (18%). Since vibriosis is known as a secondary infection caused by other stressful factors such as parasitic infections, handling and chemical treatment, it seems that cultured olive flounder are exposed to stressful environment. Viruses diagnosed in the study showed difference in distribution between wild and cultured fishes; hirame rhabdovirus (HRV) (0.1%) and lymphocystis disease virus (LCDV) (3.9%) were detected in the cultured olive flounder, but not in the wild fish, and marine birnavirus (MBV) (1.7%) and red sea bream iridovirus (RSIV) (3.2%) were detected from the wild and cultured red sea bream, respectively. From the survey conducted, it can be concluded that even though some pathogens (Trichodina sp., Microcotyle sp., etc.) are detected from both the wild and cultured fish, pathogens such as Anisakis sp., Vibrio sp. and LCDV showed difference in distribution in the wild and cultured host of same fish species and this can be attributed to their environmental condition and feeding.
Monitoring of the mortalities in the aquaculture farms of South Korea
Kim, Jin Woo ; Lee, Han Na ; Jee, Bo Young ; Woo, Sung Ho ; Kim, Young Jae ; Lee, Mu Kun ;
Journal of fish pathology, volume 25, issue 3, 2012, Pages 271~277
DOI : 10.7847/jfp.2012.25.3.271
The practical monitoring was performed to survey the mortalities in aquaculture farms of olive flounder, rockfish, rainbow trout, Japanese eel, white shrimp and abalone in South Korea from May to November, 2011. The aquatic organism disease inspectors, who have the national licenses for the diagnosis and prevention of aquatic disease and have close relationship with the farms, investigated the rates and causes of mortalities according to the standard manual. In 70 flounder farms, the cumulative mortalities rate was 27.9%, and the mortalities were caused by scuticociliatosis, streptococcosis, VHS, non-infectious loss, vibriosis and gliding bacterial disease. The moralities rate of 30 rockfish farms was 13.6%, and those were mainly contributed by gill flukes and streptococcosis. Most of mortalities of rainbow trout were caused by non-infectious loss and protozoan white spot disease. The mortalities rate of Japanese eel was 0.6% by edwardsiellosis, protozoan white spot disease and gill flukes. The loss rate of white shrimp was 71.2%, and most of them was related with non-infectious ones, such as carnivalization, transportation loss, and the rest was caused by viral white spot disease. The mortalities rate in the abalone farms was 10.7% and all of them were related with non-infectious loss.