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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 26, Issue 3 - Dec 2013
Volume 26, Issue 2 - Aug 2013
Volume 26, Issue 1 - Apr 2013
Selecting the target year
Hematological constituents and ultrastructural changes in dark-banded rockfish, Sebastes inermis, under nitrite stress
Park, In-Seok ; Goo, In Bon ; Kim, Young Ju ; Choi, Jae Wook ; Oh, Ji Su ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 1~9
DOI : 10.7847/jfp.2013.26.1.001
The acute toxicity and sublethal effects of nitrite on the dark-banded rockfish, Sebastes inermis (mean body weight:
g), were studied under static conditions for a period of 96 h. The acute toxicity of nitrite was at the 50% lethal concentration (
) of 700 mg/L. The sublethal effects on selected hematological parameters of the dark-banded rockfish, such as its osmolality, hematocrit, cortisol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were measured after 0, 6, 12, 24, 48, 72, and 96 h of exposure to 0, 50, 100, 200, 400, or 700 mg/L nitrite. Sublethal nitrite caused a progressive reduction in the hematocrit of the fish, depending on the nitrite concentration and the exposure period. Exposure to 100-700 mg/L nitrite for 96 h caused a reduction in the hematocrit and an increase in cortisol, ALT, and AST compared with the control levels. Abnormal ultrastructural changes in the gills and liver tissues were observed in fish exposed to 700 mg/L nitrite for up to 96 h compared with the control tissues. Ultrastructural changes included atrophic gill mitochondria and hepatocytes that developed smooth endoplasmic reticulum and atrophic mitochondria. Although no rockfish mortality occurred at 500 mg/L nitrite, all the hematological parameters examined responded adversely to a nitrite dose of 200 mg/L for 96 h. These results show that although the acute toxic concentration of nitrite for the dark-banded rockfish is > 700 mg/L, sublethal concentrations of nitrite also negatively affect its hematological parameters.
Effects of Cadmium on Embryo Hatchability, Larval Development and Survival of the Olive flounder, Paralichthys olivaceus
Min, Eun Young ; Kang, Ju-Chan ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 11~17
DOI : 10.7847/jfp.2012.26.1.011
The cadmium (Cd) toxicological effects on the fertilized eggs, embryos and larvae were investigated in olive flounder, Paralichthys olivaceus water-borne exposed to Cd. The survival rate and hatching success of the embryos significantly diminished in treated groups in dependence of the Cd concentration. Significant differences were found at
exposed groups compared to the control group. A significant increase of malformation of the embryo was observed at
exposed groups. They usually include such symptoms as clouded yolk-sac abnormality, fin erosion and spinal curvature. A significant reduction in the survival rate of the larvae was observed in
exposed groups with accompanied by the disorder. Notably, in larvae, a concentration as low as
exposed groups caused significant elevated abnormalities that is incidences of spinal cord deformation, abnormal eyes, deformation of the head region and severe developmental delay.
Cloning and Expression of the Cyclooxygenase-2 gene in the Rock bream, Oplegnathusfasciatus
Jin, Ji Woong ; Kim, Do Hyung ; Kim, Young Chul ; Jeong, Hyun Do ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 19~30
DOI : 10.7847/jfp.2012.26.1.019
Megalocytivirus is a major fish pathogen in marine aquaculture of Asian countries including Korea. Despite of many species affected by this pathogen, little is known interaction between megalocytivirus and the fish immune system. One of the cyclooxygenase isoforms, named COX-2, is playing an important role in immune regulation, and distinct from COX-1 isoform of constitutive activity. COX-2 enzyme is induced by various inflammatory signals, including injection of lipopolysaccharide or infection by pathogenic agents. We cloned COX-2 gene in rock bream using degenerated primers designed from reported sequences of other fish species in PCR followed with 5'- and 3'-end RACE-PCR. The full length of cDNA of rbCOX2 (rock bream COX-2) gene are 2655 bp and that translates into 609 amino acids. The rbCOX-2 genomic organization are found to span 10 exons separated by 9 introns. We also studied if the experimental infection of rock bream with megalocytivirus could affect the expression of COX-2 gene. When injected with LPS, expression of the COX-2 gene was reached peak level at 1 day post injection and showed 13.10 fold increased level compared with that of control. While, when injected with megalocytivirus, we were not able to find significantly increased COX-2 gene expression different from that of control. Cloned and analyzed COX-2 gene in rock bream will help to understand defence mechanisms in fish after viral infection and will also support the development of the measures for treatment and prevention of viral infection.
Comparison of microscopic counting and alamar blue assay to evaluate anti-protozoal effects against Azumiobodo hoyamushi that causes soft tunic syndrome to Halocynthia roretzi
Lee, Jae-Geun ; Zeon, Seung-Ryul ; Park, Kyung-Il ; Choi, Sang-Hoon ; Park, Kwan Ha ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 31~38
DOI : 10.7847/jfp.2012.26.1.031
The edible ascidian, Halocynthia roretzi is a commercially important fisheries resource in Korea. However, there have been outbreaks of mass mortality due to soft tunic syndrome. It was discovered recently that the cause of death is infection by a protozoan parasite Azumiobodo hoyamushi. Alamar blue assay and microscopic counting were used to estimate anti-protozoal effects of 20 drugs having different action mechanisms. Through comparison of alamar blue assay and microscopic counting, 6 drugs were found to be potential in protozoan-killing effects: amphotericin B, formalin, hydrogen peroxide, bithionol, benzalkonium chloride, bronopol (24hr-
). The preliminary data can be used as a basis to develop anti-protozoal agents against A. hoyamushi.
Identification of water mold from wild brook lamprey, Lethenterone reissneri
Kim, Hyoung Jun ; Park, Jeong Su ; Kim, Sung Yeon ; Koo, Ja Geun ; Bang, In-Chul ; Kwon, Se Ryun ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 39~44
DOI : 10.7847/jfp.2012.26.1.039
Saprolegnia isolate from wild brook lamprey was identified on the basis of its morphological and molecular characteristics. The isolates showed aseptic hyphae and clavate zoosporagium. Zoospores discharge was typically saprolegnoid. Neither oogomia nor antheridia was observed in this study. ITS sequence obtained from the isolate was compared with other Saprolegnia spp. to analyse their phylogenetic relationships. Results showed that the isolate belongs to clade I including Saprolegnia parasitica. Based on the asexual organs, zoospore discharge manner and ITS sequence analysis, the isolate was identified as S. parasitica.
The Residues of Enrofloxacin in Cultured Paralichthys olivaceus
Seo, Jung Soo ; Jeon, Eun Ji ; Lee, Eun Hye ; Jung, Sung Hee ; Park, Myoung Ae ; Jee, Bo Young ; Kim, Na Young ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 45~50
DOI : 10.7847/jfp.2012.26.1.045
Enrofloxacin is one of the normally used flouroquinolones in mammalian and fish but its withdrawal time and studies were remain obscure. The residual contents of enrofloxacin in fish muscle were analyzed by using HPLC-FLD. More than 0.1 mg/kg of ENR was detected in muscle tissues and the residues were found over 1 year after treatment. The concentration of ENR in Paralichthys olivaceus was not affected by water temperature and lasted for an extended amount of time. The spike recoveries of ENR in the muscle tissue ranged from 78% to 85%. From this results, we need the prescription by veterinarian or aquatic organism disease inspector in ENR usage to assure safety of fish. Future research is required to determine the recommendation dose of ENR for side effects and safety.
Exfoliation of abalone, Haliotis discus hannai using organic acid
Kim, Wi-Sik ; Lee, Si-Woo ; Kim, Jung ; Choi, Dong-Ik ; Oh, Myung-Joo ; Hwang, Doo-Jin ;
Journal of fish pathology, volume 26, issue 1, 2013, Pages 51~56
DOI : 10.7847/jfp.2012.26.1.051
It is reported that abalone, Haliotis discus hannai, was detached from shelters by commercial oxytetracycline (OTC) dissolved in hydrochloric acid (HCl). In the present study, we investigated the exfoliation effect of fouling abalone by organic acids instead of OTC or HCl. Organic acids (malic acid, citric acid, lactic acid and formic acid) of pH 2.6 and pH 2.1-2.3 exfoliated over 67.6% and 91.7% of abalone, respectively; while OTC of pH 2.6 and pH 2.1-2.3 exfoliated 25.9% and over 74.1% of abalone, respectively. These results indicate that the exfoliation effect of organic acid is better than that of OTC dissolved in HCl at the same pH. However, a lower pH and longer treatment of organic acids resulted in delayed recovery of the detached abalone; abalone immersed in pH 2.3 for 10 second was recovered within 5 min, but took 12 min to recover after 30 second immersion. Moreover, recovery period for abalone exposed to pH 2.1 for 30 second was at least 15 min 45 second. In conclusion, though acids need to be cautiously handled, organic acids may be a better candidate to detach abalone instead of OTC or HCl.