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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 26, Issue 3 - Dec 2013
Volume 26, Issue 2 - Aug 2013
Volume 26, Issue 1 - Apr 2013
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Monitoring of Kudoa septempunctata in Cultured Olive Flounder and Wild Fish in Jeju Island during 2012
Song, Jun-Young ; Choi, Jun-Ho ; Choi, Hye-Sung ; Jung, Sung Hee ; Park, Myoung Ae ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 129~137
DOI : 10.7847/jfp.2013.26.3.129
The study surveyed infection rate of Kudoa septempunctata parasitized in the trunk muscle of olive flounder, Paralichthys olivaceus, cultured in Jeju Island and wild fish species caught in the coastal area around Jeju Island during 2012. Among 143 olive flounder that were randomly sampled from 26 different culture farms, K. septempunctata was detected in 7 fish samples (4.9%) from 4 different culture farms, showing no typical Kudoa infestation. However, K. septempunctata was not detected in olive flounder fry sampled from hatcheries and 8 species of wild fish. In addition, we compared 3 different sampling sites on trunk muscle of 7 Kudoa positive fish that included head part, tail part and entire muscle. Among 7 fish, K. septempunctata was detected in 3 fish that were sampled from head part; while 4 fish from tail part of trunk muscle. However, all 7 fish were positive when sampled from entire muscle. Thus, we suggest that it will be more efficient to use entire muscle sample than sampling partial muscle parts for detection of K. septempunctata.
Infestation of the Abalone, Haliotis Discus Hannai, by the Polydora under Intensive Culture Conditions in Korea
Won, Kyoung-Mi ; Kim, Byeng-Hak ; Jin, Young-Guk ; Park, Young-Jin ; Son, Maeng-Hyun ; Cho, Mi-Young ; Park, Myoung-Ae ; Park, Min-Woo ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 139~148
DOI : 10.7847/jfp.2013.26.3.139
The genus Polydora(Polychaeta, Spionidae) includes many species well known for their activity as borers. They often become harmful invaders by reducing the growth rate and meat yield of, or inducing the mortality of commercially important mollusck, abalone, Haiotis discus hannai. In 2012, the frequency of the Polydora was observed with 5~99% in live abalone and 5.3~70.3% in dead abalone shells of abalone sea-caged aquaculture system, Korea. There are many nacreous blister in the ventral margin and inner of the infestated abalone by abalone in response to the Polydora. A worm bored two holes in the shell and come in and out for ingestion the organic matters. They are more than 40 mm in length and had outstanding palps with black bars, disc form pygidium and 4 eyespots. This is the first record for the statue of Polydora infestation of sea-caged cultured abalone, Haliotis discus hannai in Korea.
Phylogenetic Analysis of Viral Haemorrhagic Septicaemia Virus (VHSV) Isolates from Asia
Ahn, Sang Jung ; Cho, Mi Young ; Jee, Bo-Young ; Park, Myoung Ae ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 149~161
DOI : 10.7847/jfp.2013.26.3.149
Viral haemorrhagic septicaemia virus (VHSV), the causative agent of viral haemorrhagic septicaemia (VHS), is an epidemic virus of cultured olive flounder Paralichthys olivaceus in Korea. In the present study, the entire glycoprotein (G) gene including several hypervariable regions from 36 isolates of diverse geographic and host origin and 8 Korean VHSV isolates from cultured olive flounder were analyzed. Phylogenetic analysis indicated that most of Asian VHSV belong to the genotype IVa group, suggesting that they originated from a common ancestral virus. Comparative sequence analysis of the complete G protein from Korean VHSV isolates revealed 3 Korean strain-specific nucleotide residues (nucleotide number of G-region: A755, T834 and T1221). These results suggest that Korean VHSV originated from a common ancestor, but these regional specific nucleotide sequences suggest that genetic differences of VHSV are more related to geographic areas than to host fish species.
Histopathologic Characterization of Viral Pathogens in Cultured Olive Flounder, Paralichthys Olivaceus, using in-situ Hybridization Methods
Do, Jeong Wan ; Lee, Nam-Sil ; Jung, Sung Hee ; Kim, Kyung-Kil ; Choi, Hye Sung ; Park, Jeong Woo ; Kim, Yi Cheong ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 163~171
DOI : 10.7847/jfp.2013.26.3.163
Polymerase chain reaction (PCR) is the most rapid and widely used method to detect viral pathogens. However, this method does not provide histopathologic nature of the virus. In situ hybridization (ISH) with oligonucleotide probes is attractive because it is a rapid method for detection and identification of viral pathogens at sites of tissue infection. In order to understand the histopathologic characterictics of Red sea bream iridovirus (RSIV), viral-hemorrhagic septicemia (VHS) virus and viral nervous necrosis (VNN) virus to cultured olive flounder, we her applied ISH method to various kinds of olive flounder tissues with PCR-positive for these three viruses. We found that these viruses showed different tissue tropism and were detected from different cell types. Our results suggest that ISH is useful not only in rapid detection of viral pathogens but also in understanding the histopathologic characters of specific viral pathogens.
A report on the 2012 mass summer mortalities of black rockfish, Sebastes schlegeli in the Southeast Sea, Korea
Lee, Deok Chan ; Park, Yong Chul ; Jeon, Chang Yung ; Yang, Joon-Yong ; Hur, Young Baek ; Kim, Jin-Woo ; Cho, Ki Chae ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 173~183
DOI : 10.7847/jfp.2013.26.3.173
From July to early September 2012, there was mass mortality of fishes, particularly black rockfish, which were being raised in the floating fish cage along the coast of Gyeongsangnam-do. The amount of damage was 1,802,000 fishes and the causes were confirmed to be rapidly rising water temperature and repeated daily changes in water temperature. The water temperature in this area of the sea rose to the maximum
and the daily range of changes in water temperature was maximum
. As a result of investigating biological diseases of 194 fishes in 49 fishery areas, major pathogenic organisms such as red seabream iridovirus (RSIV), Vibrio sp. and Vibrio spp. or Microcotyle sp. were detected in rockfish in some fish farms. It is considered that the major causes of the mass mortality were high water temperature accompanied by repeated daily changes in water temperature, it is considered that biological diseases influenced the increase in the perish of fishes.
Mixture of Edwardsiella tarda specific Bacteriophage and Bacillus subtilis KM-1enhanced bactericidal activity against Edwardsiella tarda
Baek, Min Suk ; Hwang, Yo Sep ; Choi, Sanghoon ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 185~191
DOI : 10.7847/jfp.2013.26.3.185
The present study was performed to investigate an antibacterial activity of specific bacteriophage (phage) and Bacillus subtilis KM-1 (B. subtilis) mixture against Edwardsiella tarda (E. tarda). An appropriate number of phage showing the most effective antibacterial activity was
CFU/ml of B. subtilis 36 h post incubation. On the other hand, B. subtilis showed a dose dependant manner in inducing antibacterial activity in the presence of phage (
PFU/ml). The phage and B. subtilis mixture showed higher antibacterial activity against E. tarda than phage or B. subtilis only. These results suggest that the phage and B. subtilis mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.
Analysis of diversity of hemolytic microbiome from aquafarm of arkshell, Scapharca broughtonii
Gwon, Byeong-Geun ; Kim, Young-Ok ; Nam, Bo-Hye ; Kim, Woo-Jin ; Kong, Hee Jeong ; Kim, Bong-Seok ; Jee, Young-Ju ; Lee, Sang-Jun ; An, Cheul Min ; Kim, Dong-Gyun ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 193~206
DOI : 10.7847/jfp.2013.26.3.193
The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from these regions were rapidly reduced during the last decade due to mass mortality. The reason of this great damage has not yet been identified. To overcome this economic loss, diverse investigations were focused on environmental factors that affects in the physiology of S. broughtonii, but microbiological researches were performed insufficiently. Hemoglobin is one of the major blood component of ark shell and is damaged by some species of bacterial toxins. We concentrated on this red pigment because hemolysis could be the cause of ark shell mortality. In this study, we analyzed microbial diversity of underwater sediments in coastal regions and also existences in the body of S. broughtonii. We investigate about 4,200 isolates collected from June to September for microbial diversity of sediments and ark shell. We screened all of culturable microorganisms, and identified 25 genera 118 species, 24 genera 89 species, 30 genera 109 species and 39 genera 141 species, and selected 140 unique colonies for identification and challenge assay.
The Infection Characteristics of Vibrio scophthalmi Isolated from Olive Flounder, Paralichthys olivaceus
Kim, Su Hyun ; Woo, Sung Ho ; Lee, So Jung ; Park, Soo Il ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 207~217
DOI : 10.7847/jfp.2013.26.3.207
Recently high mortality of cultured olive flounder, Paralichthys olivaceus occurred frequently at the fish farms in Ulsan, Korea. The diseased fish showed skinny body and swimming behavior around the water surface with liver atrophy and white enteritis as internal signs. The isolated bacteria were identified to V. scophthalmi by biochemical test, nucleotide analysis of 16S rRNA and dnaJ gene sequencing. The pathogen of this study showed strong pathogenicity as 75% mortality to olive flounder by intraperitoneal injection of
CFU/fish. The pathological sign was not different between the naturally diseased fish and the artificially infected fish. Histopathological changes were shown to liver atrophy, desquamation of the intestinal mucosa and hyaline droplet like as other previous studies.
Changes of Growth and Hematological Constituents in the Rock Bream Oplegnathus fasciatus Exposed to TBT
Hwang, Un-Gi ; Kim, Jun-Hwan ; Kang, Ju Chan ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 219~229
DOI : 10.7847/jfp.2013.26.3.219
Experiments were carried out to investigate the growth and hematological parameters of rock bream Oplegnathus fasciatus exposed to several different concentrations (0, 1, 2, 4,
) of tributyltin (TBT) for 4 weeks. Growth rate of the fish exposed to
of TBT concentration was significantly lower than that of control group fish. The major hematological findings were significant decreases in the red blood cell count, hematocrit value and hemogobin concentration in the fish exposed to
. Although serum glucose concentration was significantly reduced compared to the control group, total protein concentration was found to be significantly increased over the control group. Exposure to
of TBT concentration resulted in significant increase in the enzyme activities, such as glutamic oxalate transminase and glutamic pyruvate transminase in the fish. The present findings suggest that exposure to
concentration of TBT can cause significant changes in growth and hematological parameters of rock bream Oplegnathus fasciatuso.
The immune-adjuvant effect and safety of recombinant CC chemokine 1 (rRbCC1) in rock bream, Oplegnathus fasciatus
Kwon, Mun-Gyeong ; Kim, Ju-Won ; Hwang, Seong-Don ; Kim, Eun-Gyeong ; Park, Dae-Won ; Park, Chan-Il ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 231~240
DOI : 10.7847/jfp.2013.26.3.231
Adjuvants are immune enhancers that are often used in vaccination to augment the immune response of a vaccine, thereby enhancing the protective immunity against the targeted disease. In the present study, we used the recombinant protein, such as rRbCC1, this protein was produced from rock bream CC chemokine 1. To verify the adjuvant effects of this recombinant protein, the immune responses of rock bream to Streptococcus iniae (S. iniae) FKC vaccination, which alone or in combination with recombinant protein was analyzed and then also performed experimental challenge with live S. iniae. The result of serum agglutination titres was showed relatively low levels however, the efficacy of FKC vaccine still conferred protection against S. iniae. Moreover, the adverse effects result showed that no statistically significant difference was revealed between high concentration injected and non-injected fish groups, generally. The relative percent survival (RPS) of FKC + recombinant vaccination group was significantly higher than that of vaccinated group with FKC alone. After experimental challenge to the rock bream by injection with live bacteria (S. iniae), the FKC + rRbCC1 vaccination group was showed 87.0% RPS, however, the RPS of FKC alone vaccination was 68.2%. The results indicated that the recombinant protein as an adjuvant had a clear synergism to injection vaccine of rock bream.
Production and Characterization of vitellogenin monoclonal antibody on the Scorpion fish Sebastiscus marmoratus
Kim, Young-Ju ; Lim, Yoon-Kyu ; Yeo, In-Kyu ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 241~254
DOI : 10.7847/jfp.2013.26.3.241
In order to establish bio-marker systems for the screening of endocrine-disrupting chemicals contaminated in various environment, Vitellogenin(Vtg) bio-marker have been developed to detect Scorpion fish`s(Sebastiscus marmoratus) Vtg. Vtg has been induced by administration of estradiol into S. marmoratus, and purified by gel filtration and ion-exchange chromatography from serum of the fish. After immunization of the purified Vtg into BALB/c mouse, hybridomas secreting anti-Vtg antibodies have been produced. The size of induced Vtg in the serum was about 440 kDa by gel filtration using Sepharose CL-6B. By SDS-PAGE analysis, the main band of Vtg, however, was at 175 kDa, and several minor bands have been detected with the main band. Eight different monoclonal antibodies have been produced from established hybridomas and the antibodies did not cross-react with sera from different species of fishes tested in this study except with that of Sebastes hubbsi. These results suggested that the monoclonal antibody of S28 and S15 can used as capture and tracer antibodies for ELISA and ICG assays. The detection systems developed in this study can be used as Bio-marker assays to check endocrine disrupting activity of various chemicals as well as to detect known endocrine disrupting chemicals contaminated in environment.
Gene analysis of galectin-1, innate immune response gene, in olive flounder Paralichthys olivaceus at different developmental stage
Jang, Min Seok ; Lee, Young Mee ; Yang, Hyun ; Lee, Jeong-Ho ; Noh, Jae Koo ; Kim, Hyun Chul ; Park, Choul-Ji ; Park, Jong-Won ; Hwang, In Joon ; Kim, Sung Yeon ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 255~263
DOI : 10.7847/jfp.2013.26.3.255
The innate immune response is fundamental defense response of vertebrates and invertebrates. Especially, the innate immune response important for larvae that lack of resistance to infectious diseases in the early stages. Galectin is one of the kinds of lectin and presents in the fish mucous that involves innate immune response. Galectin have been studied from various fishing species, but expression analysis of galectin is still unclear during early developmental stage in olive flounder. In this study, we investigated gene expression of galectin-1 from various developmental stage and tissues. We excised several tissues including the muscle, fin, eye, gill, brain, stomach, intestine, kidney, spleen and liver from adult olive flounder and confirmed gene expression of galectin-1 using RT-PCR and quantitative real-time PCR. Expression of galectin-1 was significantly higher in muscle, stomach and intestinal tissue than other tissue in adult fish (5 and 29 months). Also, galectin-1 gene was detected from 0 DAH and gradually increased to 35 DAH and since then decreased after stomach development period. Induction of galectin-1 during the early developmental stage suggest that muscle, fin and eye tissue is formed and begins the secretion of galectin this period. In addition, increased expression levels at 35 DAH suggest that due to complete formation of stomach and intestine, increase of secretion and activation of enzyme. This study shows that expression of galectin-1 during early developmental stages and adult period in olive flounder and can be expect that galectin-1 play essental role in the innate immune system throughout the whole life time. Galectin-1 is primary barrier such as skin and digestive tissue against pathogen infection, also digestive tract developmental period is important for pathogen invasion can be expected that it will serve. Mass mortality due to the disease in seed production is continuing damage, therefore these result will be meaningful about infectious disease during early developmental stages as a basic data for the study.
A Study on the Growth and Disease of Chondrus ocellatus in Korea
Lee, Soon Jeong ; Park, Myoung-Ae ; Ogandaga-Maranguy, Cyr Abel ; Park, Seo Kyoung ; Kim, Hoikyung ; Kim, Young Sik ; Choi, Han Gil ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 265~274
DOI : 10.7847/jfp.2013.26.3.265
The growth, reproduction, gametophyte(G)/tetrasporophyte(T) ratio, and diseases of Chondrus ocellatus populations were examined at 3 sites (Samcheok, Youngduk, Pohang) of East coast and at 2 sites (Hakampo, Manripo) of West coast in between July and August, 2013. Average plant lengths were 6.10~9.69 cm and it was minimum at Manripo and maximum at Pohang population. In general, average plant length and weight of C. ocellatus were greater on East coast than West coast populations. The proportion of vegetative plant was between 26.7~66.7 %, and G/T ratio of total plants including vegetative plants after testing resorcinol method was 3:2 on the East coast where is gametophyte dominant area. However, G/T ratio was 1:1 at Hakampo and 1:2.3 at Manripo representing tetrasporophyte dominance. In the present study, Korean C. ocellatus have various diseases (white colour and green colour), an endophytic alga(Ulvella sp.), and many epiphytic macroalgae and diatoms. Healthy C. ocellatus plants were about 20~40 % in summer population and most of plants had disease. Chondrus ocellatus had a filamentous green alga, endophytic Ulvella sp. which was not identified. The endophyte is easily observed in C. crispus growing in Europe and Canada and it is recognised as a pathogen destroying population and reducing yield of C. crispus. Thus more interest and research on the endophytic algae and disease of C. ocellatus are required.
Effects of a Commercial Activating Treatment Agent on Cultured Porphyra yezoensis thalli
Park, Sung-Woo ; Kim, Dae-Hyun ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 275~282
DOI : 10.7847/jfp.2013.26.3.275
The use of activating treatment agent (formerly acid treatment agent) has been an effective strategy to remove deleterious epibiont organisms such as diatoms and green seaweeds, and it has greatly contributed to increase in Porphyra production. Although many manufacturers supply many kinds of activating treatment agent with different components in these days, no report about their effects on Porphyra culture was found. In this paper, effects of a commercial activating treatment agent were evaluated for practical use in Porphyra culture. No difference was found in dead cell ratios(%) of Porphyra yezoensis thalli between treated and control groups. However, dead cell ratios of Monostroma nitidum thalli were increased from 0~4.6% to 99.0~100% after the treatment. Bathing Porphyra thalli in activating treatment agent resulted in a great decrease in epiphytic bacterial number attached to the thalli from
cells/g but did not change the colour of the thalli. These results suggest that bathing Porphyra thalli in activating treatment agent could be a promising strategy to remove green algae, diatoms and bacteria.
Susceptibility of marine medaka Oryzias dancena to fish pathogenic viruses
Kim, Wi-Sik ; Oh, So-Young ; Oh, Myung-Joo ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 283~287
DOI : 10.7847/jfp.2013.26.3.283
The susceptibility of marine medaka, Oryzias dancena to fish pathogenic viruses (infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicemia virus (VHSV), hirame rhabdovirus (HIRRV), infectious hematopoietic necrosis virus (IHNV), and lymphocystis disease virus (LCDV)) was investigated. The cumulative mortalities of fish immersed with IPNV (experimental condition:
sea water (SW)), VHSV (
SW), HIRRV (
fresh water (FW)) were 30%, 40% and 60%, respectively. In the fish immersed with IPNV (
FW and SW), VHSV (
FW and SW), HIRRV (
SW), IHNV (
FW and SW), LCDV (
FW and SW,
FW and SW), and mock-challenged group, mortality rate was less than 10%. IPNV, VHSV and HIRRV were re-isolated from the dead fish. These results suggest that marine medaka is susceptible to IPNV, VHSV and HIRRV, although their susceptibility depends on the environmental conditions.
Characteristics of Enterococcus faecium isolated from rough-toothed dolphin, Steno bredanensis
Kim, Myoung Sug ; Choi, Hye Sung ; Han, Hyun Ja ; Kim, Doo Nam ; An, Du Hae ; Jung, Sung Hee ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 289~294
DOI : 10.7847/jfp.2013.26.3.289
Enterococcus faecium was isolated from the internal organs of the rough-toothed dolphine, Steno bredanensis which was stranded at Jeju island. E. faecium were isolated from the liver, spleen, kidney, heart and lung up to
cfu/g. No significant differences of bacterial enzyme activities between E. faecium KCCM 12118 and the isolates were found. Biochemical constellation was the same or similar to that of E. faecium KCCM 12118 according to API20 strep. All isolates had the multi-drug resistance to 6 antibiotics by an agar disk diffusion method but these isolates didn`t have resistance to chloramphenicol and vancomycin.
Molecular cloning and expression analysis of a ferritin H subunit from rock bream, Oplegnathus fasciatus
Kwon, Mun-Gyeong ; Jeong, Ji-Min ; Kim, Ju-Won ; Park, Chan-Il ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 295~301
DOI : 10.7847/jfp.2013.26.3.295
Ferritin is an evolutionarily conserved protein that plays an important role in iron storage and detoxification. In this study, the gene encoding a ferritin H subunit homologue (RbFH) was cloned from rock bream (Oplegnathus fasciatus) and analyzed at the expression. The full-length ferritin H cDNA was 1162 bp long and contained an open reading frame (ORF) of 531 bp that encoded 177 amino acid residues with a predicted molecular mass of 20.8 kDa. The 5` UTR was 297 bp in length, and the 3` UTR 298 bp, and preceded by a 5`-untranslated region that contains a putative Iron Regulatory Element (IRE). The deduced amino acid sequence of RbFH shares extensive sequence identities with the H ferritins of a number of fish species and contains the ferroxidase center that is preserved in ferritin H subunits. Examination of tissue specific expression indicated that RbFH expression was most abundant in PBLs, RBC, liver and muscle.
Histopathology of a acanthocephalan infection in swamp eel, Monopterus albus
Kang, Hye Min ; Lee, Hanna ; Yim, Sang Gu ; Kim, Young Dae ;
Journal of fish pathology, volume 26, issue 3, 2013, Pages 303~310
DOI : 10.7847/jfp.2013.26.3.303
Since March in 2013, Inland Aquaculture Research Center, NFRDI has cultivated 1,000 wild swamp eel(Monopterus albus) for species conservation research. While cultivating, 100 fishes showed clinical sign that darkness color, mucus hypersecretion and anus rubor. Even some of them were died. Result of anatomical test, all 100 fishes were infected with intestinal parasite, acanthocephalan. So we were going to determine the case of acanthocephalans infection in swamp eel(Monopterus albus) as histopathologically. Acanthocephalan was founded in alimentary canal only. Parasite were confirmed 19 unit in individual fish, averagely. Heavy infected fishes were confirmed enterocleisis by acanthocephalans. Worms were attachment in submucosa layer of alimentary canal by invading proboscis. Characteristic symptoms were observed in stomach and intestine, including hyperemia in mucous epithelium, infiltration of eosinophills in submucosa layer, inflammation, parasitic granuloma. Some fishes showed vacuolization of gastricgland epithelium, necrosis of intestinal mucosa. Other organs, excluding alimentary canal, were not found lesion. The results of this study, the effect of the acanthocephalan infection on swamp eel(Monopterus albus) could find and it seems to be a big help in the future swamp eel(Monopterus albus) cultivation.