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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 27, Issue 2 - Aug 2014
Volume 27, Issue 1 - Apr 2014
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Acquired resistance of rock bream (Oplegnathus fasciatus) against rock bream iridovirus (RBIV) through undergoing low water temperature period
Zenke, Kosuke ; Yoon, Ki Joon ; Kim, Min Sun ; Choi, Seung Hyuk ; Kim, Ki Hong ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 85~89
DOI : 10.7847/jfp.2014.27.2.085
Water temperature is a key environmental factor controlling the epizootics of viral diseases in fish. High water temperature is associated with the rapid spread of rock bream iridovirus (RBIV) disease and with high mortality of RBIV infected fish. Although protection of fish against iridoviral disease by active immunization has been reported, little information is available concerning whether fish survived from an epizootic of iridoviral disease can naturally acquire resistance against the viral disease. In the present study, we have demonstrated that juvenile rock bream, which survived from a natural epizootic of RBIV, acquired resistance against recurrence or reinfection of RBIV, and this resistance was established during the subsequent low water temperature period. Furthermore, the possible involvement of the adaptive humoral immune response in the resistance of the juvenile rock bream was suggested by in vivo neutralization experiment.
Monitoring of Legally Designated Disease in Cultured White Shrimp, Litopenaeus vannamei in Korea (2010~2013)
Kim, Su-Mi ; Choi, Min-Ji ; Kim, Seok-Ryel ; Kang, Seo Kyeong ; Hwang, Hye Yeon ; Jang, In-Kwon ; Kim, Jin Woo ; Jee, Bo-Young ; Shin, Ki-Won ; Park, Myoung Ae ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 91~97
DOI : 10.7847/jfp.2014.27.2.091
Since the "Aquatic life disease control act" was established in 2009, we have monitored OIE notifiable and legally designated diseases which are associated with white shrimp Litopenaeus vannamei but are as yet outbreak in South Korea. We had monitored only two viral diseases of YHD and IMN, but further added IHHN, TS and WTD in an attempt to reinforce monitoring as a countermeasure against the increasing possibility of imported diseases led by continuous growth in global fisheries trade. We also increased the number of monitoring areas, and shrimp farms. In 2013, we examined a total of 2,650 white shrimp from 29 hatcheries and farms to check whether they were infected with any of the 5 diseases (YHD, IMN, IHHN, TS, WTD). The result showed that none of the samples contained the viruses. To regulation of the exotic diseases from landing in our country and to strengthen prevention, management and control of the diseases on a national level, we must continue the surveillance monitoring of the diseases.
Development of piezoelectric immunosensor for the rapid detection of marine derived pathogenic bacteria, Vibrio vulnificus
Hong, Suhee ; Jeong, Hyun-Do ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 99~105
DOI : 10.7847/jfp.2014.27.2.099
Biosensors consist of biochemical recognition agents like antibodies immobilized on the surfaces of transducers that change the recognition into a measurable electronic signal. Here we report a piezoelectric immunosensor made to detect Vibrio vulnificus. A 9MHz AT-cut piezoelectric wafer attached with two gold electrodes of 5mm diameter was used as the transducer of the QCM biosensor with a reproducibility of
in frequency response. We have tried different approaches to immobilize antibody on the sensor chip. Concerning the orientation of antibody for the best antigen binding capacity, the antibody was immobilized by specific binding to protein G or by cross-linking through hydrazine. In addition, protein G was cross-linked on glutaraldehyde activated immine layer (PEI) or EDC/NHS activated sulfide monolayer (MPA). PEI was found to be more effective to immobilize protein G following glutaraldehyde activation than MPA. However, hydrazine chip showed a better capability to immobilize more IgG than protein G chip and a higher sensitivity. The sensor system was able to detect V. vulnificus in dose dependent manner and was able to detect bacterial cells within 5 minutes by monitoring frequency shifts in real time. The detection limit can be improved by preincubation to enrich the bacterial cell number.
Inhibitory Effects of Candidate Probiotic Bacteria on the Growth of Fish Pathogenic Bacteria, Streptococcus sp.
Lee, Minyeong ; Kim, Eunheui ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 107~114
DOI : 10.7847/jfp.2014.27.2.107
For the treatments and protection of bacterial fish disease, many requirements are needed for aquatic probiotics so that they are effective in aquaculture animals but are also harmless to humans. In the present study, among 17 candidate probiotic bacteria (CPB) obtained from the edible part of the shellfish, Bacillus sp. CPB-St (CPB-St) were selected and in vitro evaluated for the possibility as a probiotic strain for the control of fish streptococcosis which frequently occurs in the olive flounder farms. CPB-St showed inhibitory effects on the growth of various fish pathogenic bacteria, Streptococcus sp., S. parauberis, S. iniae, Lactococcus garvieae and L. piscium by the double layer test ranging about 18~26 mm of clear zone. Inhibitory activity of CPB-St to Streptococcus sp. was observed 6 hours after and the growth of Streptococcus sp. was decreased to 8~55 folds in the co-culture of CPB-St with Streptococcus sp.. The safety of CPB-St to fish and survival of CPB-St in the intestine were assessed in the olive flounder, Paralichthys olivaceus. Fish mortality was not observed in artificial infection with CPB-St for 2 weeks. CPB-St was entirely excreted from the stomach and intestine 24 hours after oral injection. This results indicate that CPB-St has potential applications as a probiotic for the control of fish streptococcosis in aquaculture.
Thermal effects on antioxidant enzymes response in Tilapia, Oreochromis niloticus exposed Arsenic
Min, EunYoung ; Jeong, Ji Won ; Kang, Ju-Chan ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 115~125
DOI : 10.7847/jfp.2014.27.2.115
The effects of waterborne arsenic (As) exposure on antioxidant defense were studied in liver and gills of tilapia, Oreochromis niloticus under thermal stress. Tilapia were exposed to different As concentrations (0, 200 and
) at three water-temperatures (WT; 20, 25 and
) for 10 days. In antioxidant response, glutathione (GSH) levels, glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-treansferase (GST) activities were significantly decreased depend on WT in the gills after As exposure. Also, the range of fluctuation in these enzymes activities was most significantly increased at
in the liver of tilapia exposed to As. The present findings suggest that a simultaneous stress by temperature change and As exposure could accelerate the alteration in antioxidant enzymes activities of tilapia.
Evaluation of tagging of olive flounder, Paralichthys olivaceus for stock enhancement
Kwon, Mun Gyeong ; Seo, Jung Soo ; Hwang, Jee Youn ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 127~131
DOI : 10.7847/jfp.2014.27.2.127
The suitable tag was investigated based on the cumulative mortality, blood chemistry and histopathology of olive flounder, Paralichthys olivaceus. The dissection of opercle, 50% dissection of pelvic fin and 100% dissection of pelvic fin was used in the experiment. Cumulative mortality of dissection of opercle group was high. The dissection of opercle and 100% pelvic fin dissection groups was showing histological changes after 13days of tagging: purulent inflammation in the liver, fibrous inflammation in the body kidney, hyperplastic ellipsoide capillary in the spleen. As the results, 50% pelvic fin cutting group is the most effective out of them.
Vibrio anguillarum infection in rainbow trout Oncorhynchus mykiss during seawater adaption
Kim, Si-Woo ; Kim, Jong-Oh ; Kim, Wi-Sik ; Kim, Do-Hyung ; Oh, Myung-Joo ;
Journal of fish pathology, volume 27, issue 2, 2014, Pages 133~137
DOI : 10.7847/jfp.2014.27.2.133
We examined the cause of a disease outbreak in rainbow trout Oncorhynchus mykiss, which were adapting to a seawater in an aquaculture farm in Jeju on April, 2013. Most of the diseased fish showed a severe ulcer on the skin, enlarged spleen, expanded stomach and hemorrhage of abdominal and pyloric region. Although no parasites, fungi or viruses were isolated from diseased fish, over 200 same bacterial colonies were isolated from liver, spleen and kidney. Nucleotide sequences of the 16S rDNA gene of the bacterium in this study showed 100% identity with Vibrio anguillarum. This study is the first report of V. anguillarum infection in rainbow trout during sea adaption in Korea.