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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 29, Issue 1 - Apr 2016
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Isopod Parasite Induced Secondary Microbial Infection in Marine Food Fishes
Ravichandran, S ; Sivasubramanian, K ; Parasuraman, P ; Rajan, D. Karthick ; kumar, G. Ramesh ;
Journal of fish pathology, volume 29, issue 1, 2016, Pages 1~5
DOI : 10.7847/jfp.2016.29.1.001
Isopods are parasitic crustaceans that pose serious threat to fisheries. Several studies have tried to explore the host-pathogen relationship between marine fishes and isopods. The present study aims to understanding the secondary infections in marine fishes pertaining to isopods. To assess the secondary infection in infected fishes, parasite infested and healthy tissues of fishes were collected. The samples were subjected to standard microbiological procedure to identify the presence of pathogenic bacteria and fungi. Our results showed the branchial region had the higher microbial load of non-sporulating cenocytic fungi in infected fishes. Moreover, fungal strains isolated from the parasitic lesion confirmed that the parasitation and body lesion facilitates the entry of several pathogenic microbes at the damaged host tissue. More over the immune regulation of fish fights back by producing minute cysts, trying to encapsulate the growing fungus. But this may eventually lead to systemic infestation and death of the fish.
Light and electron microscopic observations of Ceratomyxa sparusaurati (Myxosporea: Bivalvulida) from the gall bladder of rock bream (Oplegnathus fasciatus)
Cho, Jae Bum ; Kim, Ki Hong ;
Journal of fish pathology, volume 29, issue 1, 2016, Pages 7~12
DOI : 10.7847/jfp.2016.29.1.007
In a previous study on the parasites of cultured rock bream (Oplegnathus fasciatus), we reported the discovery of a new species, Ceratomyxa oplegnathus, obtained from the gallbladder. In the present study, we found another Ceratomyxa species, C. sparusaurati, also from the gallbladder of rock bream. The morphological and ultrastructural characteristics of C. sparusaurati were investigated using light and transmission electron microscopy (TEM).
Transcriptional analysis of olive flounder lectins in response to VHSV infection
Lee, Young Mee ; Noh, Jae Koo ; Kim, Hyun Chul ; Park, Choul-Ji ; Park, Jong-Won ; Noh, Gyeong Eon ; Kim, Woo-Jin ; Kim, Kyung-Kil ;
Journal of fish pathology, volume 29, issue 1, 2016, Pages 13~23
DOI : 10.7847/jfp.2016.29.1.013
Lectins play significant roles in the innate immune responses through binding to pathogen-associated molecular patterns (PAMPs) on the surfaces of microorganisms. In the present study, tissue distribution and expression analysis of olive flounder lectins were performed after viral hemorrhagic septicemia virus (VHSV) challenge. Fish egg lectin and serum lectin were found to be predominantly expressed in the gills and liver, these results indicate that the transcript expression of olive flounder lectins is concentrated in immune-related tissues. Following a VHSV challenge, an overall increase in the transcript levels of the genes was observed and the expression patterns were distinctly divided into early and later responses during VHSV infection. In conclusion, olive flounder lectins are specifically expressed in immune-related organs and induced in both the immediate and long-lasting immune responses to VHSV in the olive flounder. These results indicate that lectins may be play important roles in the host defense mechanism and involved in the innate and adaptive immune response to viruses in fish.
Effects of acetaminophen administration on liver histopathology, serum GOT/GPT levels and circulating microRNA-122 concentration in olive flounder (Paralichthys olivaceus)
Najib, Abdellaoui ; Kim, Dong Soo ; Kim, Ki Hong ;
Journal of fish pathology, volume 29, issue 1, 2016, Pages 25~33
DOI : 10.7847/jfp.2016.29.1.025
In human medicine, circulating microRNAs have been successfully utilized as early biomarkers for various abnormalities and disease states. Vertebrate miR-122 is a liver-specific microRNA which is expressed almost solely in hepatocytes and plays an important role in the regulation of hepatocyte function. In this study, to evaluate the potential utility of circulating miR-122 as a biomarker for liver injury in olive flounder (Paralichthys olivaceus), fish were orally intubated with two doses of acetaminophen (500 mg/kg or 1.000 mg/kg of body weight), and the expression of miR-122 in serum was quantified using real time-PCR. Histological change in liver, and the enzymatic activity of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) were also analyzed. The results showed that miR-122 was higher in acetaminophen administered groups compared to control group. The histopathological effect of acetaminophen on olive flounder liver was not distinct. The serum level of GPT and GOT was increased within 2 folds compared to control group by acetaminophen administration. However, the serum miR-122 level was increased more than 3 or 4 folds compared to the control group by administration of 1000 mg/kg of acetaminophen. These results suggest the possible use of miR-122 as an indicator of liver injury in olive flounder, even when histopathological effects are not distinctive.
Potential use of Bacillus amyloliquefaciens as a probiotic bacterium in abalone culture
Park, Jin Yeong ; Kim, Wi-Sik ; Kim, Heung Yun ; Kim, Eunheui ;
Journal of fish pathology, volume 29, issue 1, 2016, Pages 35~43
DOI : 10.7847/jfp.2016.29.1.035
In comparison to the numbers of such studies of fish, few studies have been carried out on the immunity, physiology and ecology of abalone, while studies on abalone disease are also extremely rare. Moreover, mass mortality of cultured abalone due to pathogenic bacteria has not been reported in the southern coast of Korea. However, Vibrio-like bacteria have been isolated from dead abalone, which indicates that a review is required in order to determine the cause of abalone mortality. Use of an antimicrobial agent to minimize the damage caused by disease in abalone farms is common, but the therapeutic effects are insignificant. Demand for probiotics has increased, but research on the development of probiotics for use in abalone culture is very rare. Therefore, the present study isolated KC16-2 from fermented kimchi soup and investigated the characteristics of the isolate as a candidate probiotic bacterium in abalone. KC16-2 was identified as Bacillus amyloliquefaciens (B. amyloliquefaciens KC16-2) based on its biochemical properties and 16S rRNA gene sequence. B. amyloliquefaciens KC16-2 showed inhibitory effects against the growth of various vibrios in vitro, and kept the numbers constant until four days after inoculation in marine water at a temperature of
, indicating the possible use of KC16-2 as a probiotic, except in the winter. The growth of KC16-2 was inhibited by bile salt, but the numbers increased over time suggesting the bacteria were still alive in the abalone's digestive tract. Abalone fed with a diet including KC16-2 for 12 weeks showed good growth, but showed no significant differences from the control group. However, the mortality of the abalone supplied the probiotic diet was reduced to half that of the control group in a challenge test with Vibrio tubiashii. Therefore, we suggest that B. amyloliquefaciens KC16-2 could be used as a probiotic bacterium for control of the mortality of abalone caused by opportunistic pathogenic vibrios.