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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 7, Issue 2 - Dec 1994
Volume 7, Issue 1 - May 1994
Selecting the target year
A new species of Bonnierilla (Copepod, Cyclopoida, Notodelphyidae) parasitic on Halocynthia roretzi (V. Drasche) from the Kamak Bay, Korea
Choi, Sang-Duk ; Hong, Sung-Yun ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 83~94
Bonnierilla namhaesius n, sp, is described based on the specimens recovered from the ascidians, Halocynthia roretzi Von Drasche in Namhae Islands, Korea, This is distinguished from congeners by having a combination of characters : setal formula 3, 17+1 hook, 9+1 aesthete, 5, 3, 2, 2+1 aesthete, 7+1 aesthete respectively on eight segments of antennule, II, 5 on distal segment of the second leg to fourth leg exopod, and 2, 3, I on distal margin of caudal ramus. This is the second record of the male, and first record of the copepodid in the genus Bonnierilla.
Characterization of growth hormone-like sequence of loach, Misgurnus mizolepis
Kim, Jin-Kyung ; Song, Young-Hwan ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 95~103
We have prepared cDNA libray of loach. M. mizolepis in order to isolate cDNA clone of growth hormone gene. Total RNA was isolated from pituitary of loach, and then mRNA was further purified from total RNA by oligo (dT)-coupled magnetic beads. The purified mRNA was used as substrates to prepare cDNA. The resulting cDNA was ligated into the EcoRV/Smal site of pBlueKS+. The ligation mixture have transformed E. coli JM109 strain with electroporator to obtain high yield of transformation efficiency. All the transformants was screened with DIG-labeled Tilapia growth hormone gene by high density colony hybridization. After isolating 10 putative colonies showing the positive signals, secondary colony hybridization and southern hybridization could confirm it as true clones. The nucleotide sequence of one candidate, pCGHI, was compared with 312 bp DNA fragment used as DNA probe and show 52% relative homology to Tilapia growth hormone gene.
Studies on disease of catfish, Silurus asotus, in Korea. III. Edwardsiella ictaluri infection.
Park, Sung-Woo ; Kim, Young-Gill ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 105~112
A new bacterial infection occurred among the cultured Korean catfish, Silurus asotus, in Chunbuk prefecture, Korea, 1993. This disease produced about 30% mortality in the fish for 4 months. The diseased fish was swimming listless at the water surface with head up and tail down, sometimes spinnig in circles. The most outstanding clinical sign was ulceration on the skull and at the base of the pectoral fins. The causative organism was isolated from the brain, kidney, spleen and liver of diseased fish, and identified as Edwardsiella icaluri by the biochemical and biophysical characteristics. After intraperitoneal innoculaton of the isolate, the pathogenicity was proved positive for Korean catfish, S. asotus, and channel catfish, Ictalurus punctatus, but negative for carp, Cyprinus carpio.
Antimicrobial activity of Artemisia princeps var. orientalis essential oil against fish pathogenic bacteria
Kim, Young-Gill ; Rho, Bum-Jin ; Lee, Keun-Kwang ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 113~117
The antimicrobial activity of Artemisia princeps var. orientalis essential oil against a partial fish pathogenic bacteria was examined. The growth of Aeromonas hydrophila, Aeromonas salomonicida, Aeromonas sorbia, Edwardsiella tarda and Streptococcus sp. (yellowtail) were inhibited at concentrations of 1,000 to 2,000 ppm. The A. salmonicida was inhibited at 1,000 ppm, A. hydrophila, A. sorbia, E, tarda and Streptococcus sp. (yellowtail) at 1,500 ppm, but Vibrio anguillarum, Vibrio ordalii, Edwardsiella ictaluri and Streptococcus sp. (SF 1) were grown on 100-2,000 ppm.
Effect of water temperature on the immune response of flounder, Paralichthys olivaceus
Bang, Jong-Deuk ; Park, Soo-Il ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 119~126
To investigate effects of water temperature on immune response of flounder, Paralichthys olivaceus, against Edwardsiella tarda, fish were immunized with formalin killed E. tarda antigen, and humoral immune response of these fish were observed. At lower water temperature (12 and
), the antibody appeared 2 to 3 weeks after injection of formalin killed E. tarda antigen and the maximum agglutination titer was 16 and 32, respectively. However at higher water temperature (20 and
), the antibody appeared one week after injection and the maximum agglutination titer was about 2,048. Once produced agglutination titer was sensitively responsed to variation of water temperature and showed that this phenomenon had also a similar tendency under natural condition. And it showed that agglatination titer of flounder immunized with formalin killed E. tarda maintained above 19 months.
Histopathological studies on melano - macrophage centers (MMCs) in spleen and head kidney of immuno - modified tilapia, Oreochromis niloticus
Park, Jeong-Hee ; Huh, Min-Do ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 127~149
Histopathological studies on the two lymphomyeloid organs of spleen and head kidney in tilapia, Oreochromis niloticus, were carried out to clarify the significance on the morphological characteristics of melano - macrophage centers (MMCs) which are varied in different physiological and pathological conditions of teleosts. To examine the histological changes by the artificial modification of the immunological states, tilapia were treated intraperitoneally with FKC and LPS of Edwardsiella tarda, and orally with dexamethasone, and then followed by the intraperitoneal injection of colloidal carbon for chasing the macrophages. There were marked differences in phagocytic avidity of macrophages, and accumulating patterns of carbon - ladening macrophages into the MMCs among the test groups. In the non - pretreated control group, carbon - ladening macrophages were densely accumulated at 12th and 20th day within the MMCs of head kidney and spleen, respectively. And, in the groups treated with bacterial antigens (FKC & LPS), the macrophages were more rapidly and densely aggregated within MMCs. But in the group with dexamethasone, only a few carbon particles were detected in both organs. Any compactly isolated form of particles was not found in this group. From the present results, it was strongly suggested that certain changes in immunological states of tilapia influence on the morphology of MMCs including the frequency of appearance, sizes, aggregating patterns or outlines. Therefore, morphology of MMCs would be very important in the interpretation for histopathological findings seen in the teleost's lymphomyeloid organs.
Histopathological changes in fingerlings of Japanese flounder, Paralichthys olivaceus, with severe scuticociliatosis
Lee, Nam-Sil ; Park, Jeong-Hee ; Han, Kyu-Sik ; Huh, Min-Do ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 151~160
In order to elucidate the patterns of tissue damage evoked by the scuticociliatids, eighteen fingerlings of Japanese flounder, Paralichthys olivaceus, heavily infected with an unidentified scuticociliatid were histopathologically examined. Skin layer with the underlying musculature were severely necrotized due to the infestation of the ciliates. However in the early lesions, both dermis and myofibres remained relatively intact compared with other surrounding loose connective tissues. Mild damages were found in more dense tissues. One or more scuticociliatids were recognized in the blood and lymph vessels of the loose connective tissues with or without destructive changes. Many of nerve trunks or ganglia were also parasitized with less marked histological damage in the parenchyma. Dura and its adjacent tissues in the spinal cord were severely necrotized with massive accumulation of the ciliates in subdural space. The parasitic invasion in the central nervous system was usually confined to the cortical region. In the gill, variable degenerative changes were occurred due to the invasion of the ciliates recognized in the blood vessels of branchial arches or primary filaments. From these results, it was strongly suggested that the scuticociliatids are very actively penetrated into the deep tissues mainly through the severe destruction of the loose connective tissue components and that the vascular system could play a role in the rapid distribution of the ciliates to the remote tissues or organs.
An investigation of the congestion on the gills of eel
Kim, Young-Gill ; Choi, Min-Soon ; Park, Sung-Woo ; Lee, Keun-Kwang ;
Journal of fish pathology, volume 7, issue 2, 1994, Pages 161~171
In order to elucidate the outbreak mechanisms of a new disease which is characterized by an intense congestion in central venus sinuses(CVS) of gill filaments in cultured eel. these experiments were carried out; epidemically surveyed on the cultured eel farms in the vicinity of Kunsan city and experimentaliy outbreaked the disease in the stressful condition such as thermal and handling shock and innoculated the supernatant from the homogenate of naturally severe congested gill into eels and onto the monolayer of the CHSE-214. Although the frequency of congestion in eels of B, C, D and E farms were higher than in eels of A farms, the water qualities(stocked and cultured water) among farms were not a great difference. In eels of B, C, D and E farms, the values of haematocrit(Ht), haemoglobin(Hb), total protein(Tp), albumin(Alb), glucose(Glu), magnesium(Mg) were lower and the values of calcium(Ca), methemoglobin(Met-Hb), glutamic pyruvic transminase(GPT), glutamic oxalacetic transminase(GOT) higher than in eels of A farms. These valules have not related to the frequency of congestion. An intensive congestion and dilataton in CVS of gill filaments in experimentally handling-stressed eels produced similar histopathological changes to those observed in the spontaneously diseased eel, but not in eels experimentally injected with filtering contents. The cytopathic effect on the CHSE-214 was not observed. In stressed eels the congestion of gill was increased in relation to either the decrease ranges of water temperature or the incerase in accllimated times. And increase in Ht, Met-Hb, Alb, Glu, GOT and GPT and decrease in Mg, Hb and Tp were found, which had a close relationship to congestion of gill. Cortisol were increased according to the decrease ranges in acclimated water temperature. From these results, decrease in water temperature during selection placed eels upon the stressed condition, made increase in ionic strength in blood stream, and CVS was dilatated owing to the increased blood inflow.