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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of fish pathology
Journal Basic Information
Journal DOI :
The Korean Society of Fish Pathology
Editor in Chief :
Volume & Issues
Volume 8, Issue 2 - Dec 1995
Volume 8, Issue 1 - May 1995
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Two species of copepods (Crustacea) parasitic on marine fish, Konosirus punctatus, from Kamak Bay in Korea
Choi, Sang-Duk ; Hong, Sung-Yun ; Rho, Yong-Gil ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 1~11
Two species of the parasitic copepods, Nothobomolochus thambus (Poecilostomatoida, Bomolochidae) and Mitrapus heteropodus (Siphonostomatoida, Lernanthropidae), were recovered on the gills of a marine fish, Konosirus punctatus (Temminck and Schlegel) taken from Kamak Bay in Korea. N. thambus is very distinguishable in the armature of maxilliped from all other species of Nothobomolochus; one of the two strong, hairy setae has become naked. M. heteropodus is very distinguishable in the armature of leg 4; the inner process (endopod) is only about one - fifth the length of the outer process (exopod), Both parasitic copepod species are new to the Korean fauna.
A study on Actinosporeans parasitized in Branchiura sowerbyi (Oligochaeta : Annelida)
Kim, Young-Gill ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 13~21
Four species of Actinosporeans, Aurantiactinomyxon sp. A, B, C and Neoctinomyxon sp. D were released from Oligochaete, Branchiura sowerbyi captured from three reserviors, where intestinal giant-cystic disease in carp had occured from June to September, 1994. All part of the intestinal epithelial tissue from the gullet to the anus of B. sowerbyi were infected by Actino-sporeans, and many mature Actinosporean were seen more easily at the posterior parts of the body. Just before releasing, mature Actinosporean sporozoites were divied into each individual from the intestinal epithelial tissue of Oligochaete, while immature ones had 6 spores (
in size) per each in the oocyst (
in size). A total of 1, 762 of B. sowerbyi were investigated in three reservoirs, 86 individuals (4.88%) of them were infected; 0. 74% (13 ind.) of Aurantiactinomyxon sp. A, 2. 27% (40 ind.) of Aurantiactinomyxon sp. B, 1. 59% (28 ind.) of Aurantiactinomyxon sp. C, and 0. 28% (5 ind.) of Neoactinomyxon sp. D. At the room temperature of 22.6-
, number of extrusion dates of Actinosporeans from B. sowerbyi for 32 days are 1 day (23.3% of total, 1 time) or 5 days (11.7%. 5 times), and the majority was finished within 15 days, however, 6.7% of total were released for 32 days.
Studies on parasitic trematodes in bivalves -Variation of infection rate of Cercaria tapidis and Cercaria harengulae-
Kim, Young-Gill ; Lee, Keun-Kwang ; Kim, Young-Jin ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 23~30
We had reported the infection rates of trematode larvae, Cercaria tapidis and C. harengulae in the gonad tissues of Tapes philippinarum and Solen strictus in the estuary of Kum river, the west coast in Korea in 1980~1982. At the same sites from January 1994 to January 1995, we investigated the variation of the infection rates of these trematode larvae in the two clams. The results are follows. The infection rate of C. tapidis parasitized in T. philippinarum increased in 2.4 times (14.0%) higher than that (5.7%) in 1981. Monthly maxium infection rate was 32.0% in October 1994. Comparing the rate (23.3%) in December 1981, the rate in 1994 showed a higher trend than that in 1981. The mean infection rate of C. harengulae in T. philippinarum showed 2.3% in 1994. Comparing that (5%) in 1980, it showed a decreased rate than that in 1980. And monthly maximum infection infection rate of this clam was 6.7% in May 1994, compared with the rate (19.0%) in March 1980. It appeared furthermore decreased rate in 1994. The mean infection rate of the year of C. harengulae in S. strictus was 6.9% in 1994, while it was 10.2% between 1981 and 1982. It showed a decreased trend in 1994. Monthly maximum infection rate was 23.4% in August 1981, while it was 13.3% in April 1994. It showed a decreased trend in 1994 also. In case of C. tapidis the more large sizes of shell length the more infection rates were higher as same in the investigation in 1980, while in C. harengulae the smaller sizes, the more infection rates showed a higher trend.
Molecular cloning and foreign gene expression of restriction endonuclease fragments of the Hc nuclear polyhedrosis virus DNA
Lee, Keun-Kwang ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 31~36
Hc nuclear polyhedrosis virus DNA genome was digested with EcoRI endonuclease, these partial fragments were recombined into the pUC8 plasmid vector and transformed in E. coli JM 83 cell. The genome DNA has 24 EcoRI fragments and 12 fragments of them were subcloned. The four recombinants were named as eNP-O, eNP-Q, eNP-R and eNP-S. The expression of foregin gene of the recombinants was investigated by analysing protein patterns on the SDS-PAGE. The eNP-O, eNP-Q and eNP-R were expressed a different weight of protein as comparision with potypeptide bands of E. coli JM 83 host cell.
The amino acid analysis of polyhedrin and DNA sequence of ployhedrin gene in nuclear polyhedrosis virus
Lee, Keun-Kwang ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 37~46
The amino acid analysis of polyhedrin protein and nucleotide sequence of polyhedrin gene in H. cunea nuclear polyhedrosis virus (HcNPV) genome have been studied. Polyhedrin had three polypeptide bands in SDS - polyactylamide gel electrophoresis. The major polypeptide had a molecular weight of 25 kd. The polyhedrin was composed of 17 different amino acids. HcNPV DNA was digested with EcoRI restriction enzyme and hybridized with (
) -labelled AcNPV polyhedrin gene cDNA. The polyhedrin gene was located on the fragment of EcoRI-H. The EcoRI - H fragment containing polyhedrin gene was cloned into the EcoRI site of pUC8 vector which was confirmed with southern blotting, and the recombinant plasmid containg polyhedrin gene was designated as hPE-H. The promoter region of polyhedrin genomic DNA was sequenced. The sequences identified as the TATA box was found at the 5' flanking region of the polyhedrin genomic DNA approximately -79 bp upstream from the transcriptional start site. But CAAT-like box was not shown near the TATA-like box in the polyhedrin gene. Four tandem repeats with the sequence 5' -CTAATAT-3' and 5'-TAAATAA-3' were found between -141 and -108 or -83 upstream and -52 bp downstream from the translation start site. About -141 bp region upstream from the translational start site was highly AT (78%) rich. The coding region for the polyhedrin starts and ends with ATG and TAA, respectively.
Studies on classification of Korean catfish (Silurus asotus) peripheral leucocytes
Park, Sung-Woo ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 47~55
Classification of peripheral leucocytes in Korean catfish (Silurus asotus) were performed. The leucocytes were classified on the basis of their morphological, cytochemieal and phagocytic characteristics. Four types of leucocytes were observed in the peripheral blood. Neutrophils were round and 12 -
in diameter with round, oval or 2 - 3 segmented neuclei. Their cytoplam was stained whitish or grey. Lymphocytes were round and 5.5 -
in diameter with large round neuclei which occupied most of the cells. Monocytes were round or irregular cells and 12 -
in diameter with bean - shaped or irreular neuclei. Thrombocytes were oval or spindle - shaped cells with oval or spindle - neucle. The cells with greyish cytoplasm ranged 7.2 to
in length and 5.8 to
in width. Only neutrophils showed positive reaction in peroxidase and PAS. Neutrophils and monocytes showed active phagocytosis. As the results it is concluded that peripheral leucocytes of Koran catfish could be classified as lymphocytes, neutrophils, monocytes and thrombocytes.
Acute toxicity effets of formaline to flounder, Paralichthys olivaceus
Park, In-Seok ; Kim, Hyung-Bae ; Kim, Min-Suk ; Park, Chul-Won ;
Journal of fish pathology, volume 8, issue 1, 1995, Pages 57~67
Twenty-four hours, acute toxicity and the histopathological effect of formaline to flounder, Paralichthys olivaceus larvae were examined. The
values obtained to farmaline were 2,520 ppm in 1 hour treatment. 1.610 ppm in 2 hours treatment, 868 ppm in 4 hours treatment and 141 ppm in 24 hours treatment. Many pathological features such as hypertrophy of mucous and epithelial cells in secondary gill lamella, hyaline droplet degeneration of tubular epithelial cells in the proximal convoluted segment of renal tubules, focal or massive necrosis in liver cells and pycnotic nucleus in heart cells were recognized. The above results were discussed in relation to the application of formaline as therapeutic agent in flounder disease.