Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korean Journal of Food And Nutrition
Journal Basic Information
Journal DOI :
The Korean Society of Food and Nutrition
Editor in Chief :
Volume & Issues
Volume 7, Issue 4 - Dec 1994
Volume 7, Issue 3 - Sep 1994
Volume 7, Issue 2 - Jun 1994
Volume 7, Issue 1 - Mar 1994
Selecting the target year
Purification and Characterization of a Pretense Actinidin Isolated from Cheju Kiwifruit
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 87~94
A protease, actinidin, was isolated from Cheju kiwi fruit Actinidia chinesis. The enzyme was purified about 8.5 fold with the yield of 25% by column chromatographies of DEAE-Toyopearl and Sphadex. G-100. Purified enzyme gave a single protein band on polyacrylamide gel electrophoresis and its molecular weight estimated by SDS-PAGE was about 27, 000. The optimum pH and temperature were 7.0 and 4
, respectively. This enzyme was stable at the ranges of pH 5.0~9.0 and below 5
. It was also found that Fe+2, Fe+3, and Na+ ions increased enzyme activity, whereas Hg+2 and Co+2 ions decreased. The enzyme was inhibited by phenylmercuric acetate and leupeptin, which indicated that active center of the emzyme had thiol-group. The enzyme reaction followed the Michaelis-Men-ten dkinetics with the Km value of 0.32 mM for casein.
Fatty Acid Composition of Korean Pumpkins
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 95~99
In order to investigate the fatty acid composition of Korean pumpkins, this study was designed. Three samples of Korean pumpkins, A(yellowish ripe), B(unripe, 30 days old) and C(unripe, 20 days old) were used for fatty acid analysis by gas chromatography. The results were as follows : Pumkin C, B and A showed 10, 12 and 15 kinds of fatty acids, respectively. In case of palmitic acid(C16:0), pumpkin C, B and A showed 22.5%, 22.9% and 26.6%, respectively. In case of u linolenic acid(C18:3), pumpkin C, B and A showed 22.6%, 23.6% and 44.8%, respectively. In case of palmitoleic acid(Cl6:1), pumpkin C. B and A showed 12.4%, 10.1% and 0.5%, respectively. In case of oleic acid(C18:1), pumpkin C. B and A showed 9.2%, 7.7% and 2.8%, respectively, In case of stearic acid(C18:0), pumpkin C, B and A showed B.S%, 3.7% and 3.5%, respectively. The ratio of P/S was 1.4, 1.6 and 1.9 for sample C, B and A, respectively. The ratio of w-3/w-6 was 1.1, 0.9 and 2.6 for sample C, B and A, respectively. Through this study, the ripe pumpkin(sample A) was thought to be good enough in nutritional aspects of fatty acids, particularly a-linoleic acid, Cl8 : 3(u-3) series.
Effect of Cnidi rhizoma Water-Extracted Solution on Fat Contents in Plasma, Liver and Adipose, and Fecal Steroids of Fatted Rats
Seong, Tae-Su ; Son, Gyu-Mok ; Bae, Man-Jong ;
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 100~107
This study was carried out to evaluate the effect of Cnidi rhizoma (CR) water extract on fat accumulation in fatted rats induced by the oral high fat administration for six weeks. Total cholesterol, neutral lipid and epididymal fat pad of CR groups were lower than the control group. On the other hand, high density lipoprotein(HDL) -cholesterol was somewhat higher In CR than In the control group. In addition, the same result as the above case was obtained when examining adipose for liver and epididymal fat components. During the feeding experiment, level of low density Lipoprotein(LDL) and very low density Lipoprotein (VLDL) increased while HDL was decreased. The degree of LDL and VLDL was decreased however, and HDL was, increased In CH groups as compared with the control group. Insulin and cortisol were higher than the control group, due to the fat accumulation. Experiment group showed the increased excretion as compared with the control group, in the excretion of bile acid and neutral sterol. The liver of the control group observed by the light microscope appeared to the fatty liver, but CR groups showed some improvement of the fatty liver. Based on the above results, it was shown that It Is possible to improve fat accumulation induced, by high fat dietary through using the oral administration of Cnidi rhizoma boiling extract solutions.
Effect of Cnidi rhizoma Boiling Extract Solution on Enzyme and Hormone of Plasma, and Liver in the Fatted Rats Induced by High Fat Dietary
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 108~113
This study was carried out to evaluate the effect of Cnidi rhizoma (CR) water extract on fat accumulation In fatted rats induced by the oral high fat administration for six weeks. To accomplish this evaluation, the serum and liver tissue have been examined for enzyme activity, cortisol and insulin level. The change of liver or tissue have been observed by the light microscope. GOT GPT and LDH activities were lower than the control group. Insulin and cortisol were higher than the control group, due to the fat accumulation. The liver of the control group observed by the tight microscope appeared to the fatty liver, but CR group showed some improvement of the fatty liver Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Cnidi rhizoma water extract.
Comparison in the Contents of the Nucleic Acids in Various Wines
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 114~118
In order to compare the contents of nucleic acid substances in various wines, the contents of nueleic acids were analyzed by HPLC. The contents of cytosine was found to decrease in the order of cherry wine > plum wine > dongdongju > chungha > pineapple wine > soju. The contents of guanine was found to decrease in the order of dongdonglu > chungha > pineapple wine > cherry wine > plum wine > soju. The contents of uridine was found to decrease in the order of dongdongju > chungha> cherry wine > pineapple wine > plum wine > soju. The contents of adenine was found to decrease in the order of dongdonglu > chungha > cherry wine > pineapple wine > plum wine > solu. The contents of guan oslne was found to decrease in the order of dongdongju > cherry wine > chungha > plum wine > pine-apple wine > soju. The contents of adenosine was found to decrease in the order of dongdongju > shun aha > cherry wine > plum me > pineapple wine > soju.
Aspergillus sp. BY-54가 생산하는 Dextranase의 정제 및 특성
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 119~123
Aspergillus sp. BY-54 which produced a strong dextran hydrolyzing enzyme was isolated from soil. Using this strain, the optimal cultural conditions, enzyme purification and characterization were studied. The results are as follows : The optimal concentration of dextran as carbon source was l%. and the optimum temperature and the initial pH for enzyme production was 3
, and 7.0, respectively. Dextranase was purified by DEAE-cellulose column chromatography with a linear gradient increase in NaCl. Km value of dextranase was 0.222%, and several glucans containing various types of glucosidic linkages such as DEAE-sephadex, CM-sephadex and sephadex G-100 were almost digested to a large extent with this dextranase. The enzyme was strongly inhibited by sodium fluoride, KMnO4 and p-CMB, while KCN caused 20% of activation.
Changes in Rutin and Fatty Acids of Buckwheat During Germination
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 124~127
This study was carried out to investigate the chemical change of rutin and fatty acids of buckwheat during germination. The content of rutin in buckwheat before germination was 31.5mg% and that after 7 days was increased to 1, 660.3mg%. The content of stearic acid was increased slightly after 3 day during germination but those of other fatty acids were decreased gradually.
Antioxidative Action of Enzymatic Hydrolysates of Mackerel Muscle Protein
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 128~136
Mackerel muscle protein hydrolysates, which were prepared from defatted mackerel meal by proteases such as complex enzyme, alcalase, bromelain, pancrease, pepsin, w-chymotrypsin, trypsin and papain, were tested for the antioxidative action against linoleic acid. Among proteases tested, the hydrolysates obtained from the treatment of complex enzyme, bromelain and alcalase showed higher antioxidative effects. Also, the hydrolysates showed the synergistic effects with o-tocopherol and the inhibitory effects for peroxidation of metal ions(Fe3+, Cua+) From the profiles of fractionation of the hydrolysates with Bio-gel P-2 column, the most active fractions, part I(complex enzyme-derived) and part e(bromelain-derived), had below MW 1,400 and the antioxidative effects were closely related to the binding capacity with metal ion(Cua+). Amno acid composition of the part I was abundant in histidine, arginine, phenylalanine and lysine, and the part e was abundant in lysine, glutamic acid and leucine.
Optimization for Extrusion Cooking Conditions of Rice Extrudate by Response Surface Methodology
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 137~143
To optimize extrusion cooking condition of single screw extuder for production of puffed rice extrudate using response surface methodology (RSM), moisture content, barrel temperature and screw speed were determined from contour maps, showing relationship between dependent (hardness, expansion ratio, water absorption index, water solubility Index, degree of gelatinization) and independent variables. Optimum operational conditions for production of puffed rice extrudate with suitable quality properties were moisture content 17%, barrel temperature 1
and screw speed 210 rpm, respectively.
The Contents of Amino Acids and Amylose of Ssooksulsis and Ssookjulpyuns Affected by Added Mugworts
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 144~150
Ssooksulgies and Ssookjulpyuns made with different levels of mugworts were attempted to analyze the Ingredients of amino acids and amylose The contents of total free amino acids of 30% Ssoohsulgi was 25.9mg% which was four times as much as that of 0% Ssooksulgi and the ones of total free amino acids of 30% Ssookjulpyun was 39.58mg% which was eleven times as much as that of 0% Ssookjulpyun. The more mugworts were added, the less amylose were contained.
Retarded Fermentation in Making Liquid-Yoghurts and Improvement by Addition of Na-Caseinate hydrolysates
The Korean Journal of Food And Nutrition, volume 7, issue 2, 1994, Pages 151~158
This study was carried out to clear up the cause of low-acid producing phenomenon occurred In non-fat dry milk during liquid-yoghurt fermentation by Lactobacillus case, and to present its improving methods. All samples of non-fat dry milks which were low in TCA-soluble peptides showed low-acid production, but those high in TCA-soluble peptides showed high-acid production. The addition of trypsin-hydrolysate of Na-caseinate to non-fat dry milk showed some improving effect on acid production but that of papain-hydrolysate did not show any improving effect and that of bacterical neutral protease-hydrolysate showed some inhibitory effect. The improving effects on growth and acid production of lactic acid bacteria were more prominent when the trypsin-hydrolysate of Na-caseinate was added. to such fermenting system in which the levels of TCA-soluble peptides and the proteolytic ability of starter bacteria were abnormally low. The liquid-yoghurt made with non-hydrolysed Na-caseinate and defective non-fat dry milk showed precipitate occurrence but that with trypsin-hydrolysate of Na-caseinate and defective non-fat dry milk did not make any precipitate during storage as with normal non-fat dry milk.