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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 10, Issue 1_2 - Nov 1980
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Secretory Granul Cells in the Midgut Epithelium of the Blattella germanica L.
Yu, Chai-Hyeock ; Kim, Woo-Kap ; Kim, Chang-Whan ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 1~6
The secretory granul cells in the midgut epithelium of Blattella germanica L. were observed by the electron microscope. These secretory granul cells contain many electron dense granules, and granules are about
in diameter respectively. It is easy to distinguish 3 different types of granul cells based on their shapes, location, and staining intensity: 1) The light secretory granul cells and their nucleus are both round form and a number of mitochondria, vacuoles, and other cell organelles appear in the cytoplasm. 2) The other kind of light secretory granul cells are small and oval form but ceil organelles are not well developed in the cytoplasm. This granul cell is surrounded by a few regenerative cells ('nidi'). 3) Dark secretory granul cells are cone shaped, well stained, and endoplasmic reticulum, ribosomes, and a lot of secretory granules are found in the cytoplasm. They are all located in the basal portion of the midgut epithelium.
An Improved Method for EM Radioautographic Techniques using Cork
Kim, Myung-Kook ; Hassler, R. ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 7~17
Electron microscope radioautography introduced by Liquier-Milward (1956) is now used routinely in many laboratories. Most of the technical difficulties in specimen preparation have been overcome. This method is modified from loop method for improvement of EM radioautographic techniques. The advantages of this method are: 1. the use of single specimens on small corks and of a large wire loop, allows the experimenter to avoid the blemishes in the membrane; 2. the surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4, thus greatly prolonging the period of time over which good emulsion layers can be made; 3. corks can be handled in perspex holder which allows about 20 specimens to be developed simultaneously. The steps of the method comprise: 1. Cut ribbons of ultrathin sections of silver interference colour 2. Pick them up on formvar-coated 200 mesh grids 3. Prestaining of tissues 4. Coat the specimens with a thin layer of carbon by evaporation (30-60A) 5. Mount the specimens on corks (about 1cm apical diameter) using double-sided scotch tape 6. Emulsion coating; a. Take a 250m1 beaker, place it on the pan of a sliding weight balance and weigh it. Add 10 grams extra to the beam. Add pieces of ILford L4 emulsion to the beaker until the balance is swinging freely. Add the 20ml of distilled water that was previously measured out. b. Surfactant dioctyl sodium sulphosuccinate is added to diluted ILford L4. 7. Prepare a series of membranes of gelled emulsion with the wire loop and apply one to each cork-borne specimen. 8. Put the specimens away to expose by pushing the corks into short length of PVC tubing, each tube having a small hole in the side 9. Place the tubes in small boxes together with silica gel. 10. Exposure 11. Developer - Kodak Microdol X for 3 minutes 12. Fixer - A perspex holder can be manufactured which allows 20 specimens to be developed simultaneously. 12. Fixer - 30% sodium thiosulfate for 10 minutes 13. Examination with Siemens Elmiskop 1A electron microscope
Treatment of E. coli B with two Antibiotics and their Influence on
Chung, Sang-Jin ; Kim, Woon-Soo ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 19~25
E. coli B was treated with colistin and kanamycin and the influence of these antibiotics on the absorption of
phage was studied using the plaque counting method and the electron microscope. E. coli B treated with colistin was sharply inhibited on phage absorption and cell walls were severely damaged showing some spiny appearance around the walls. No influence of kanamycin was noted on phage absorption. Bacterial cells treated with kanamycin showed wave form in the structure of walls and a profound change was noted in the cytoplasm where it was concentrated along the periphery of the inner wall leaving the center of the cell to appear almost empty.
Ultrastructural Observations of a Human Cutaneous Leishmaniasis
Seo, Young-Hoon ; Hur, Kyu-Chung ; Deung, Young-Kun ; Kim, Chung-Sook ; Lee, Yoo-Bock ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 27~32
A case of cutaneous leishmaniasis developed in a 48 year old Korean male who returned from middle east was studied by light and electron microscopic observations. Light microscopically, the lesion consisted of heavy chronic ill-defined granulomatous inflammation involving entire thickness of the dermis, composed of mainly histiocytic and small mononuclear cell infiltrations without evidence of necrosis or giant cell formation. Giemsa staining revealed numerous intracellular micro-organisms within histiocytes, showing dark stained central dot surrounded by light stained cytoplasm. Electron microscopically, the organisms were observed mostly ovoid in shape and frequently binary mitotic features within the host cells. follicle consisted of double unit membranes and microtubules, which are immediately below these membrnae. A long kinetoplast was noted within a very elongated mitochondrion at the center of the organisms and a flagella rose in front of the kineoplast but ended within the cytoplasm. Large numbers of free ribosomes, occasional Golgi complex and SER were also noted, but RER was seldom found. These ultrastructural features corresponded to promastigote stage of Leishmania tropica. In principle, leishmaniasis is a tropical disease and can not be found in temperate zone. However, travel to mideast by many Koreans may contract this disease while they are in endemic regions.
Taxonomic study of epidermal patterns on some American species Carex using Scanning Electron Microscope
Oh, Yong-Cha ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 33~40
Scanning Electron Microscopic Observation on the Rat Oviductal Epithelium in each Segment during Normal Sexual Cycle
Lee, Jae-Hyun ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 41~51
Scanning electron microscopic observation of the oviductal epithelium was carried out on the virgin white Wistar rat during normal sexual cycle, and obtained the results as follows; Rat oviductal epithelium during the normal sexual cycle generally consisted of two types of cells: ciliated and non-ciliated cell. The ciliated cells had cilia which was measured
in width and
in length. According to the length of microvilli and the distribution in each segment, the non-ciliated cell was further subdivided into four types of cells; NC-I, NC-II, NC-III, and NC-IV. The morphologic characteristics of each type of cells were also ascertained by SEM observations.
Studies on the Early Ultrastructural Alterations of Renal Tubules of Rats in Acute Mercury Poisoning
Byun, Hyo-H. ; Yazima, Gompachi ; Lee, Yoo-B. ; Kim, Chung-S. ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 53~76
Structure and function of the secretory ducts in Panax ginseng C.A. Mayer
Kim, Woo-Kap ; Kim, Eun-Soo ;
Applied Microscopy, volume 10, issue 1_2, 1980, Pages 77~86
The distribution of the secretory ducts, fine structures of the secretory epithelial cells, and the ingredients of the metaplasmic inclusions were studied at light and electron microscopical levels in seeds, stems, leaves, and roots of ginseng. The secretory ducts occurred in the hypocotyl of the embryo, in the cortex of the roots, and also both inside and outside of each vascular bundle in the stems and leaves. Especially, it is considered that the circular layers of the secretory ducts in roots may represent their ages. The epithelial cell has well developed nucleolus, mitochondria and smooth endoplasmic reticulum. Sudanophyl and osmiophilic inclusions were found in the epithelial cytoplasm and duct lumen. But these inclusions were not observed when extracted with pyridin or alcohol. In contrast to the lumen with red color, the epithelial cells were blue in color as stained with nile blue, suggesting that the former inclusions are neutral lipid while the latter are acidic lipid. The electron density of the cell inclusions was quite high as fixed with osmium tetroxide, indicating that most of these secretory materials seem to be unsaturated lipid. Therefore, since ginseng secretory ducts are closely associated with the lipid metabolism, it should be called lipid canal or lipid duct.