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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 11, Issue 1 - Jun 1981
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An Ultrastructural Study on the Nerve Cell Bodies of Subesophageal Ganglion from the Cabbage Butterfly, Pieris rapae L.
Kim, Woo-Kap ; Lee, Bong-Hee ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 1~9
The study on the nerve cells in the subesophageal ganglion of 5-day-old cabbage butterfly, Pieris rapae L., was performed to observe their ultrastructures and classify them on the basis of the differences in size, shape and relative distribution of cell organelles. 1. Type I neurons: These cells are neurosecretory granules ranging 100 to 300 nm in size. 2. Type II neurons: As giant neurons averaging 25 to
in size, such as mitochondria and Golgi apparatus. 3. Type III neurons: These spindle-shaped cells range 9 to
in width. 4. Type IV neurons: These cells have a range of diameter from 12 to
. The cells are abundantly observed in the subesophageal ganglion. 5. Type V neurons: These cells are very small nerve cells with 4.5 to
in size and have a prominent nucleus.
A Study on Gonad in Pieris rapac L. I. An electron microscopical observations on testis
Kim Chang-Whan ; Kim Woo-Kap ; Han Sung-Sik ; Lee Keun-Ok ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 11~19
Testis of Pieris rapae L. was observed by using light microscope and electron microscope. The results were as follows 1. Pieris rapae L. possesses a ovoid single testis colored red. 2. Peritoneal sheath consists of three layers; outer cuticular layer, middle filamentous layer, inner layer with no filaments. 3. Follicles enclosed by peritoneal sheath are compacted by the folds of the. epithelium of follicle. They are opened together toward the duct of vas deferens. 4. Epithelium of follicle have high electron density and contain needle shaped structures and glycogen. Tracheoles are extended into the epithelium of follicle. 5. Cyst cells have a irregular shaped nucleus and cell organelles are abundant in cytoplasm. 6. Typical acrosomes were not observed, but the other structure smillar to the acrosome of the other insect or vetebrate were observed. 7. After releasing the sperm bundle, cyst cells seem to be autolyzed.
Light and electron microscopic studies of a gastric xanthoma
Kwon Tae-Jung ; Kim Chung-Soak ; Moon Young-Myung ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 21~28
Gastric xanthoma which is not a true neoplasm and clinically insignificant consists of the small yellowish lesion of the gastric mucosa, frequently of multiple occurrence. Histologically the lesion shows chronic superficial gastritis with intestinal metaplasia and occasional collections of foam cells within the lamina propria. Electron microscopically. the xanthoma, cells are composed of. lipid-laden histiocytes with. many autophagocytic Iysosomes surrounding the cytoplasmic lipid vacuoles. Many residual bodies are also noted. The pathogenesis of the gastric xanthoma is obscure, however it is thought that a previous focal lesion of the gastric mucosa may have been a factor. One case of gastric xanthoma is reported here and a brief review of literature is also made.
Ozone Inactivation of Bacteriophage f2
Kim, Chi-Kyung ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 29~38
Bacteriophage f2 were treated with ozone at various concentrations for 20 minutes. The inactivation kinetics of f2 phage were examined during ozonation. In order to study the mode of action of ozone on the phage f2, absorption of the phage to the host pili was meassured by utilyzing radioactivity of tritium incorporated into the phage RNA. Sucrose density gradient analysis and electron microscopy were also used to prove the mechanism of ozone inactivation of the phage. Strucural proteins of the phage were broken by ozonation into many protein subunits. The extent of phage breakage was proportional to ozone concentration and reaction time. Percent decrease of the phage absorption to the host pili was coincident with the rate of ozone inactivation of the phage. Ozone inactivation of bacteriophage f2 was shown to be caused by the breakage of the structural protein and blockage of the phage absorption to the host pili.
Electron Microscopic Study on the Mast Cells of the Vertebrates(Mammals) Stomach
Kang Ho-Suck ; Kim Chang-Whan ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 39~50
An electron microscopical observation was carried out to compare the general shape of the mast cells and structures of granules inside the cells in the stomach of 5 species in 3 orders of Mammals. In convenience, the granules in the cytoplasm were abbreviated as follows: 1) Homogeneous granule, GR1 2) Particulate granule, GR2 a. Dark dense particulate granule, GR2-1 b. Less dense particulate granule, GR2-2 3) Reticular granule, GR5 a. Dark dense reticular granule, GR5-1 b. Light dense reticular granule, GR5-2 In Mammalia including goat, dog, cat, and hamster, most of cytoplasmic organelle were Golgi apparatus and mitochondria, and most of the cytoplasmic granules were highly densed GR1and GR2. However GR5-1 and GR5-2 appeared in guinea pig while one side sunken or crescent-like types occured in both dog and guinea pig. All mast cells were oval or spindle with cytoplasmic processes around the cell. There was also found vacuoles and vesicles in these cells. These results demonstrated that there was a morphological difference between species of vertebrate in the mast cells and their cytoplasmic granules. It was also suggested that a variety of structures of granules were closely related with the composition (histamine, heparin, serotonin, hyaluronic acid etc.) and mature of the granules.
Electron Microscopy Studies on the Formation of Polyhedra Occlusion Bodies of Autographa californica Nuclear Polyhedrosis Virus
Lee Hyung-Hoan ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 51~57
The process of the formation of polyhedra occlusion bodies and occlusion of viral nucleocapsids of Autographa californica Nuclear Polyhedrosis Virus in Spodoptera frugiperda cell were photomicrographed and described. Progeny viral nucleocapsids were observed in the nuclei of the host cells, bundled and then enveloped. The nucleoapsids were mainly accumulated near the membrane-like profiles. The nuclear membrane were hypertophied up to the cytoplasmic membrane. Prepolyhedral bodies were observed and they were growing with the accumulations of thread-like materials(polypeptides) produced by viral genes. The bundled and enveloped nucleocapsids were occluded into the growing polyhedra.
An Electron Microscopical Study on the Pars distalis of Rana dybowskii Guenther. II. Ultrastructural differences between hibernating and active periods of frogs
Kim Chang-Whan ; Kim Woo-Kap ; Lee Keun-Ok ; Kim Ji-Hyun ; Kim Hyong-Bai ;
Applied Microscopy, volume 11, issue 1, 1981, Pages 59~65
The pars distalis of the Korean frogs (Rana dybowskii Guenther) during hibernating and active periods was observed with the electron microscope. Seven cell types were classified according to the size and shape of secretory granules and to the ultrastructural characteristics. There were many differences between hibernating and active frogs in type 5 cells. Therefore the following results were observed. Cell type 1; This type cell contains spherical secretory granules,
in diameter. Cell type 2; This type cell contains various secretory granules,
in diameter Cell type 3; Spherical and rod-shaped granules,
in diameter were observed. Cell type 4; In this type cell, the electron density is the lowest and the density of granules is the highest of all type cells. This type cell contains various secretory granules and large secretory granules,
in diameter, were also observed. Cell type 5; The electron density of this cells is similar to that of type 4 cells. The density of granules is lower than that of type 4 cells. And the shapes of the secretory granules are similar to those of type 4 cells. But many rod shaped granules,
in diameter, were also observed. Cell type 6; This type was similar to type 2. The electron density of cytoplasm is very low. Spherical granules,
in diameter, were observed. Cell type 7; This type of cell has no secretory granules. This cell is not developed very well. The type 5 cells in hibernating frogs are different from cells in active frogs. In type 5 cells, many secretory granules were observed during active period. But the number of secretory granules were greatly declined and there were many vacuoles in cytoplasm during hibernating period.