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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 17, Issue 2 - Dec 1987
Volume 17, Issue 1 - May 1987
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Electron-Microscopic Studies on the Spermiogenesis and Spermatozoa of the Allied Rock Wallaby(Petrogale assimilus)
Kim, J.W. ; Harding, H.R. ; Shorey, C.D. ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 1~15
Testes from the allied rock wallaby(Petrogale assimilus) have been examined by the electron microscopy in thin sections in order to examine spermiogenesis and structure of spermatozoa. The spermiogenesis can be divided into nine stages: early Golgi, late Golgi, collapsing, nuclear protrusion, condensation and flattening, nuclear shaping, rotation, nuclear ring contraction, and maturation. The acrosome has been abruptly formed following the collapse of expanded acrosomal vesicle without the cap stage described in the eutheria. The flatly condensed nucleus rotates obliquely to the axis of the axial filament complex and the folded acrosome covers the anterior third of the dorsal nuclear surface forming a wide subacrosormal space as the nuclear ring has contracted. The Sertoli cell reaction and spur are prominent during the nuclear protrusion and rotation stages. A mature spermatozoon has S-shape head which has an extended part reaching to the vicinity of the middle piece.
Isolation of Carlaviruses from Some Medicinal Plants
Lee, Joon-Tak ; Doi, Yoji ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 16~28
Carlaviruses were isolated from naturally infected medicinal plants, and identified by means of test plants and electron microscopy. Mottle symptoms were shown on leaves of Panax ginseng, Aralia cordata, Xanthium strumarium, Taraxacum officinale, Aconitum carmichaeli, and Bupleurum longiradiatum var. breviradiatum. Ring spot on leaves of Abutilon avicennae and ring mosaic or vein clearing on leaves of Sambucus sieboldiana were also shown. These viruses had rather narrow host ranges by mechanical inoculation. The virus particles were scattered or aggregated in cytoplasm of infected host plant leaves. The carlaviruses for which the name panax virus S (PaVS), aralia virus S (ArVS), xanthium mottle virus (XaVS), taraxacum virus S (TaVS), aconite mottle virus (AcMV), bupleurum virus S (BuVS) and abutilon ring spot virus (AbRSV) were proposed, had flexuous particles with width 13 nm and length
. A reported elder ring mosaic virus was isolated from leaves of Sambucus sieboldiana with ring mosaic or vein-clear symptoms.
Effetcs of Hexavalent Chromium on the Mitochondrial Electron Transport System in Mouse Liver
Boo, Moon-Jong ; Yoo, Chang-Kyu ; Choe, Rim-Soon ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 29~46
To study hexavalent chromium effects on mitochondrial electron transport, the activities of electron transport enzymes and conformational change of mitochondria treated with
of sodium dichromate (
) were investigated. And so were those of liver mitochondria isolated from mouse intraperitoneally injected with sodium dichromate, 40mg per kg body weight. On both treatment with chromium(VI), the activities of electron transfer enzymes (Complex I and IV) were increased to some extent and the ultrastructural transformation of mitochondria from a condensed to an orthodox conformation was inhibited under State IV respiration. These results represent' inhibitory effect of hexavalent chromium on electron transport without inhibiting electron transfer enzymes (Complex I and IV) in mitochondria. On intraperitoneal treatment with hexavalent chromium as sodium dichromate and trivalent chromium as chromic chloride, containing 37.5 mg of chromium per kg body weight, respectively, the activities of electron transfer enzymes of liver isolated from mouse with chromium(VI) was reduced, but that with chromium(III) was not affected. And with chromium(VI), all mice after 12 hours of treatment died, only after 6 hours survived. With chromium(III), however, all survived. This indicates that hexavalent chromium is more toxic than trivalent chromiumin mouse liver.
An Ultrastructural Study on the Renal Glomeruli of the Aging Rat
Yang, Nam-Gil ; Han, Jong-Won ; Ahn, Eui-Tae ; Ko, Jeong-Sik ; Park, Kyung-Ho ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 47~64
Morphological difference of the renal glomerulus at different age groups have been studied in young (three month-old), adult (twelve month-old) and old (thirty month-old) Fisher strain 344 rats. Pieces of the tissues were taken from renal corticies prefixed with 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), following by post-fixation with 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3) and embedded within Araldite. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed under a JEM 100CX electron microscope. The mean thickness of glomerular basal lamina and Bowman's capsule were determined by measuring the thinnest portion of basal lamina, and by taking the average of 50 readings from electron micrographs at different ages. The numerical changes of the slit pores were compared based upon the numbers over the length of 10um of glomerular basal lamina. The results were as follow: 1. The thickness of glomerular basal lamina is increased during aging; 140.4 nm in young rats, 270.0 nm in adult ones, and 437.8 nm in old ones. 2. The thickness of basal lamina of parietal cells of Bowman's capsule is 187.5 nm in young rats, 914.0 nm in adult ones, and 2850.0 nm in old ones. 3. The numbers of the slit pores of basal lamina are reduced during aging, 30.3 slit pores/
in adult ones, and 24.2 slit pores/
in old ones. 4. Accumulation of dense intracytoplasmic filamentous material in the parietal cells of Bowman's capsule is increased in the vicinity of the basal lamina during aging. The proximal tubule-like epithelial cell in Bowman's capsule is observed at one glomerulus in a young rat. 5. The endothelial cells are edematous and form balloon-like structure protruding into capillary lumen in young and old rats. 6. Cytoplasm of the podocyte shows a variety of alteration during aging, such as swelling of mitochondria and of endoplasmic reticulum, and increase of microtubules, microfilaments, lysosomes and lamellated myelin structures, etc. Accumulation of dense intracytoplasmic material in the foot processes is increased in the vicinity of the basal lamina during aging. The podocytic membrane-like structures are seen in young and o]d rats. 7. The mesangial matrices and mesangial cells are increased during aging, and slight swelling of endoplasmic reticulum and Golgi cisternae in young and old rats.
Ultrastructures of the Cutaneous Chromatophores and Pigment Granule Formation in the Goldfish, Carassius auratus L. II. Leucophore and Melanophore
Moon, Myung-Jin ; Kim, Chang-Whan ; Kim, Woo-Kap ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 65~75
The ultrastructure of the cutaneous chromatophores and the origin of pigment granules in the goldfish, Carassius auratus L., are studied with electron microscope. Leucophores contain amorphous pigment granules, leucosomes and melanosomes contain oval shaped electron dense melanin pigment granules. These pigment granules are synthesized from the Golgi complexes of each chromatophores and released into the cytoplasm. Among the dermal chromatophores, another kind of dermal cells, named rER rich cells, are also observed. And pinocytotic vesicles are being formed only the inner surface of the chromatophores adhering to the rER rich cells. From these observations, such hypothesis are being possible that pigment materials are synthesized and transfered from the rER rich cells to dermal chromatophores by the pinocytosis. And next remodelizing mechanisms are carried out by the Golgi complexes of each chromatophores.
Electron Microscopic Study on Detoxication Effect of Red Ginseng Extract on Mouse Liver Injury induced by Methyl Mercury
Chung, Hee-Won ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 76~82
Detoxication effect of red ginseng extract against toxicity of methly mercury on ultrastructure of mouse liver was studied by electron microscopy. The hepatocyte of methyl mercury treatment group showed partial rupture of nucleus, mitochondrial swelling, decrease of glycogen content, and appearance of a great number of large vacuoles. While, the hepatocyte of methyl mercury-red ginseng extract treatment group showed slight mitochondrial swelling and decrease of vacuoles in size and number than those of methyl mercury treatment group.
Fine Structures of the Enteroendocrine Cells in the Duodenal Mucosa of the Hedgehog, Erinaceus koreanus
Choi, Wol-Bong ; Won, Moo-Ho ; Seo, Ji-Eun ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 83~97
In order to discriminate the enteroendocrine cell types in the mucosal epithelium of the normal duodenum of the Korean hedgehog (Erinaceus koreanus). The tissues were fixed in the mixture of 1% paraformaldehyde and 1% glutaraldehyde in phosphate buffer (pH 7.2), and postfixed in 2% osmium tetroxide (phosphate buffer, pH 7.2). They were embedded in Araldite, and the ultrathin sections were made by LKB-V ultratome following the inspection of semithin sections stained with toluidine blue-borax solutions. Ultrathin sections contrasted with uranyl acetate and lead citrate were observed with JEM 100B electron microscope. At least six types of enteroendocrine cells distributed in the mucosal epithelium of the duodenum were identified according to their morphological characteristics mainly based on the size, shape, number and electron density of the secretory granules. Type I cells had moderately developed organelles. The secretory granules were pleomorphic (
), and the granule cores with high electron density were enveloped in limiting membrane and characterized by a narrow halo. Type II cells contained an indented nucleus and well-developed organelles. The secretory granules were round (350 nm) and classified in two kinds by electron density, moderate and high. Both granules were surrounded by limiting membrane and those with high electron density showed often a wide halo. Type III cells had an indented nucleus. The secretory granules with various electron density were round (220 nm) in shape. The granules with high electron density were enveloped in limiting membrane and characterized by a narrow halo, but those with low or moderate electron density had not been observed the limiting membrane. Type IV cells contained an indented nucleus and moderately developed organelles. The secretory granules were round (180 nm) in shape, and the granule cores with high electron density were enveloped in limiting membrane and showed often a wide halo. Type V cells had a large amount of rough endoplasmic reticulum. Secretory granules with low or moderate electron density were round (230 nm) in shape, and surrounded by limiting membrane and showed a narrow halo. Type VI cells contained an oval nucleus and well-developed organelles, especially Golgi complex. The secretory granules with high electron density were round (210 nm) in shape. The granules were enveloped in limiting membrane and showed often a wide halo.
Ultrastructural Btudy on the Degranulation of Rat Peritoneal Mast Cells Induced by Horseradish Peroxidase
Cho, Byung-Pil ; Kang, Ho-Suck ; Kim, Woo-Kap ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 98~114
Degranulation of the rat peritoneal mast cell induced by intraperitoneal injection of horseradish peroxidase(HRP) was studied using light and electron microscopes. 1. Rat peritoneal mast cells in the Tyrode's buffered salt solution injected control group did not show any particular morphological changes following the specified time course. 2. Under the light microscope, the majority of mast cells observed 10 minutes after HRP injection were nearly the same as those of the control group. However, after 30 minutes, granule densities or staining properties of certain cells began to decrease and these appearances increased gradually until 12 hours after injection, at which time small groups of granules being stained pale-red or pink with toluidine blue were easily identified in the cytoplasm of many cells, and numerous extruded granuleg were scattered around these cells. 3. In the mast cells representing the early stage of degranulation induced by HRP, the electron densities of certain granules decreased as the size enlarged, and perigranular cavities were formed by perigranular membrane expansion. As a result, a thin cytoplasmic septum was formed between the expanded perigranular membrane and the cytoplasmic membrane in the cell periphery, and fusion of the adjacent perigranular membranes was observed in the inner side of the cell. 4. In some mast cells, one or two changes in the peripheral cytoplasmic septum could be seen. One was a focal rupture of the peripheral septum and the other was the formation of a saccule containing one or more vesicles. This saccule was thought to be used for granule-extrusion site and/or material absorptive apparatus judging from the morphological characteristics. 5. As the degranulation proceeded, the granule was extruded from the cell after partial rupture of the peripheral cytoplasmic septum. This phenomenon proceeded to-ward the inner side of the cell through the fused perigranular cavities, and consequently several distinct cavities containing a few unextruded membrane-free granules were formed throughout the cytoplasm after 12 hours. As a rule, the granule-extrusion sites were relatively fewer while the cytoplasmic cavities resulting from degranulation were more numerously observed. Thus, it was thought that the granule-extrusion sites tended to be restricted in the HRP-induced degranulation.
Effects of Cadmium on Enzyme Activities and Ultrastructure in Mouse Liver and Kidney
Lee, Keu-Seok ; Yoo, Chang-Kyu ; Choe, Rim-Soon ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 115~130
The present experiment was performed to investigate the acute effects of cadmium on ultrastructural and biochemical changes in mouse kidney and compare these changes with liver damage. Mouse were injected with cadmium chloride at a dose of 5 mg/kg body weight. After treatment, mouse were sacrificed at time intervals of 6, 12, 24 and 48 hours. It was observed that ultrastructural changes in mouse kidney were composed of swelling of mitochondria, dilation in endoplasmic reticulum, wrinkling at basal infolded membrane, formation of autophagosome and partial loss of microvilli in brush. border, and that ultrastructural changes in liver were mitochondrial change, dilation and deterioration of rough endoplasmic reticulum and proliferation of smooth endoplasmic reticulum. Biochemical effects of cadmium were more severe on liver than kidney. Therefore, acutely injected cadmium caused not only liver damage, but also kidney damage.
Comparative Renal Papillary and Pelvic Epithelial Morphology of the Mammalian Kidney
Kim, Jin ; Oh, Su-Ja ; Park, In-Sun ; Chung, Jin-Woong ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 131~160
Anatomical features of the renal papilla and pelvis and ultrastructures of the epithelium covering these areas in four species of mammals were studied by means of light, scanning and transmission electron microscopy. In terms of the morphology of mammalian kidney types distinguished by Sperber(1944), Pfeiffer(1968) and Schmidt-Nielsen(1977), the kidneys of animal species used in this experiment were; 1) the mouse kidney with the fornix between a long conical papilla and the funnel-shaped pelvis, 2) the guinea pig kidney with the peripelvic column and pelvic pouch between a short conical papilla and the funnel-shaped pelvis, 3) the dog kidney with the peripelvic column and pelvic pouch between the crest-shaped papilla and the funnel-shaped pelvis, and 4) the cattle kidney which is divided into multiple renculi with minor and major calyces and pelvis. The renal papilla was lined with the simple or pseudostratified columnar epithelium which covered the inner zone of the renal medulla. The epithelial cells with numerous short microvilli on the surface contained a few organelles. In the mouse, the fornix was lined with one to two cell-layered cuboidal epithelium which covered the outer zone of the renal medulla and a part of the cortex. The epithelial cells of the fornix with numerous short microvilli or microridges on the surface had well-developed organelles. In the guinea pig, the peripelvic column was lined with the simple cuboidal or low columnar epithelium which covered the outer zone of the renal medulla. The epithelial cells with numerous short microvilli on the surface contained well-developed organelles. The pelvic pouch was lined with the pseudostratified columnar epithelium which was composed of four kinds of cells; the secretory cell with small electron-dense granules (310 nm), the secretory cell with large granules (720 nm) showing various electron densities, the mitochondria-rich cell with a single cilium, and the basal cell. Pelves of the mouse and guinea pig, peripelvic column, pelvic pouch and pelvis of the dog, and minor and major calyces and pelvis of the cattle were lined with the transitional epithelium. The fusiform vesicles in the superficial cells of the epithelium were highly developed in the dog, relatively well developed in the mouse and guinea pig, and poorly developed in the cattle. From the above findings, it is suggested that the transport of solutes and water of the urine in the pelvic cavity can take place through the epithelia covering the renal papilla and fornix of the mouse, papilla and peripelvic column of the guinea pig, and papillae of the dog and the cattle. And specialized cell types in the epithelium of the guinea pig pelvic pouch, two kinds of secretory cells and mitochondria-rich cell with a single cilium, could have peculiar functions in the renal pelvis, respectively.
Studies on the Conducting Cells in the Haustorium of Cuscuta australis R. Brown
Lee, Kyu-Bae ; Park, Jong-Bum ; Lee, Chai-Doo ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 161~168
The elongated filamentous haustorial cells, hyphae, of Cuscuta australis R. Brown penetrated into the vascular tissues of the host plant Trifolium repens L. were studied by the light and electron microscopes. The hyphae reached the host xylem were invaded into the host vessels and then they grew within the host vessels. Finally these hyphae were differentiated into the water conducting elements, xylary hyphae, by thickening of the secondary walls. The hyphae reached the host phloem were branched at the apical regions. These hyphae possessed thin-layered cytoplasm involving the typical features of sieve elements such as the parallel arrays of smooth endoplasmic reticulum, plastids with the clusters of fine starch granules, and mitochondria with the dilated cristae. It was indicated that these hyphae were differentiated into the nutrients conducting elements, phloic hyphae. The structures described were compared with those of other parasitic plants and were discussed in view of the translocation of materials from host to parasite.
Ultrastructures of Canine Transmissible Venereal Turner Cells at Stages of Maturation and Regression
Park, Nam-Yong ; Rhee, Young-Hwan ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 169~176
Naturally occurring canine transmissible venereal tumors of genital organs in mature and regressive stages from 6 dogs were examined by transmission electron microscope. The tumor cells at the stage of maturation were comprised of large round and ovoid cells with prominent nuclei and nucleoli, a few spindle-shaped cells, and irregularly shaped cells. The mature round cells were characterized by the presence of a central ovoid to irregularly round nucleus with a large eccentric nucleolus, vesicular endoplasmic reticulum, round to oval swollen mitochondria with few cristae, Golgi's apparatus, and plasma membranes with numerous microvilli. As the tumor degenerated, the tumor cells were increased in the number of spindle-shaped, fibroblast-like and irregularly shaped cells, collagen bundles, and mainly lymphocytes, in contrast to those of the stage of maturation. Regressing tumor cells were characterized by the swelling and vacuolation of mitochondria and endoplasmic reticulum, membrane-bound granules, lamellar complex, tubular structures, and dense bundles of collagen. It was suggested that transformation might occur in the course of tumor growth causing morphological change from the round to the fibroblast-like cells, and that there was the evidence of cell-mediated tumor cell lysis by lymphocyte infiltration.
An Ultrastructural Study on Endocrine Cells in the Pyloric Region of the Korean Hedgehog(Erinaceus koreanus)
Lee, Jae-Hyun ;
Applied Microscopy, volume 17, issue 1, 1987, Pages 177~184
Endocrine cells in the pyloric region of the hedgehog were studied ultrastructurally. 4 types of endocrine cells classified as gastric-type EC, ELC,
, and G cells were observed in this region. The gastric-type EC cells contained pleomorphic granules with high electron density and highly dense bodies in a dense matrix. ECL cells were characterized by the presence of round or oval granules with high electron density. Some granules of ECL cells showed a small amount of content or empty.
cells contained round and small granules with low electron density. They occasionally showed a narrow halo between the limiting membrane and the dense materials. G cells were characterized by the presence of round or oval granules with low electron density. Some granules of these cells showed a narrow halo between the limiting membrane and the core.