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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 17, Issue 2 - Dec 1987
Volume 17, Issue 1 - May 1987
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Immunoelectron Microscopic Observation on the Stratified Squamous Epithelial Cello of Pemphigus vulgaris
Lee, Cha-Soo ; Picut, Catherine-A. ; Wilkinson, J.-Erby ; Lewis, Robert-M. ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 1~22
The Fine Structure of Collar Cell in Tentacles of Chinese Mystery Snail, Cipangopaludina chinensis malleata Reeve
Song, Yong-Jik ; Kim, Woo-Kap ; Kim, Chang-Whan ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 23~30
There are groups of large monopolar cells containing numerous secretory inclusions in the muscular and connective tissue. These cells contain the electron-lucent vesicles in the size of
, which are secreted to the exterior of the tentacle through their cytoplasmic processes projected to the epithelial layer. Secretory material can be seen accumulating in swollen portions of the granular endoplasmic reticulum. It is postulated that this material is transported to the Golgi-complex and thus the limiting membrane of the inclusions is derived from the Golgi-membrane. An electron lucent material secreted from these cells seems to be similar to fibrous electron lucent material filled in the brush border of the epidermis.
An Ultrastructural Study of Tentacular Retractor Muscle of Chinese Mystery Snail, Cipangopaludina chinensis malleata Reeve
Song, Yong-Jik ; Kim, Woo-Kap ; Kim, Chang-Whan ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 31~40
The tentacular retractor muscle has many arrays of muscle fiber bundles under the epithelial layer. Most of muscle fiber bundles are arranged in parallel to the longitudinal axes of muscle fibers and a small number of them perpendiculary to them. These smooth muscle cells are filled with compactly arranged myosins and actins. These microfilaments, when the tentacle is protracted, keep abreast with straight for-ward-lined shapes while these microfilaments, when it is retracted, with curved shapes. The foldings in the sarcolemma of the muscle cell, when the tentacle is retracted, lead to the formation of normal subsurface tubules along with which a few mitochondria are included. It is thought that the formation of the sarcolemmal differentiation like the subsurface tubules has a close relation with the protraction and retraction of the tentacle. Mitochondria are found throughout the muscle cell, and sarcoplasmic reticulum (SR) developed greatly in the exoplasm close to the sarcolemma and associated with the cell membrane. Dense bodies are distributed irregularly and thin filaments are scattered around the thick filament in cross-sections, but the thin filaments may be arranged in complete or partial orbits around thick filaments. Complete orbits are infrequent.
Effects of Lead on Enzyme Activities and Ultrastructure in Cerebral Cortex
Lee, Seo-Eun ; Yoo, Chang-Kyu ; Choe, Rim-Soon ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 41~54
This experiment was performed to investigate the acute and chronic effects of lead on cerebral cortex. In acute treatment, mouse were injected with lead acetate at dose of 0.3 mmole/kg body weight, and in chronic treatment, mouse were supplied 0.03 M lead acetate sol. in the place of water. After treatment, mouse were sacrificed at time intervals of 24, 48, 72, and 96 hours in acute treatment and at time intervals of 4 weeks and 8 weeks in chronic treatment. In acute treatment, acetylcholinesterase activity is reduced at 72 hours and recovered at 96 hours in homogenate, and reduced at 24 hours and recovered at 72 hours in crude synaptosomes. In chronic treatment, acetylcholinesterase activity is increased in young mouse but reduced in mother mouse. Ultrastructural changes were composed of swelling of Golgi apparatus, nerve terminals with diminished synaptic vesicles, and vacuolated myeline lamellae of myelinated axon.
Effects of Lead on the Ultrastructure ana the Electron Transport System of Mitochondria of Mouse Kidney
Lim, Seung-Sub ; Yoo, Chang-Kyu ; Choe, Rim-Soon ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 55~71
To investigate the effects of lead on the electron transport system and ultrastructure of mouse kidney mitochondria, various lead acetate concentrations were treated in vitro and respiration rate, enzyme activities were measured. Ultrastructural changes at state IV respiration were also observed. To compare with in vivo experiments, mouse were injected intraperitoneally of 100 mg lead acetate per kg body weight and state IV respiration rate and enzyme activities were measured. Ultrastructure of renal proximal tubular cells were also observed. In in vitro treatement, decreased state IV respiration, decreased enzyme activities, ruptured membranes and inhibition of condensed to orthodox transformation were observed. In in vivo treatment, decreased state IV respiration and decreased enzyme activities were observed after 24 hrs of i.p. injection. Cytochrome c oxidase activity showed twice the inhibition compared to NADH-CoQ reductase activity at 24 hrs. Continuous decreased state IV respiration was observed after 48 and 72 hrs of injection, however, the enzyme activities were increased to control level. Lead-protein complex which probably inhibits the toxic effects of lead appeared. To conclude, dominant effect of lead on the electron transport system appeared at cytochrome c oxidase activity, and the increased enzyme activities may be a result of appearance of lead-protein complex.
Histochemical and Ultrastructural Studios on the Cutaneous Mucous Glands of the Water Toad, Bufo stejnegeri Schmidt
Do, Kum-Ryung ; Moon, Myung-Jin ; Kim, Chang-Whan ; Kim, Woo-Kap ;
Applied Microscopy, volume 17, issue 2, 1987, Pages 72~84
Histochemical and ultrastructural properties on the cutaneous mucous glands of the water toad, Bufo stejnegeri Schmidt, are studied with light and electron microscopes. Mucous glands of the water toad are simple alveolar glands which are composed of gland cavity in dermis and gland duct in epidermis. Each gland cavity of the mucous glands is consisted of outmost myoepithelial cells, glandular epithelial cells and inner cavity. Gland cavities of the mucous glands are composed of four kinds of glandular epithelial cells which are the dark cell, the light cell, the mucous containing cell and the small cell. Secretory materials which are certified as acid mucopolysaccharides by the histochemical reactions are synthesized through the Golgi complexes of the mucous containing cells.