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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 19, Issue 2 - Dec 1989
Volume 19, Issue 1 - May 1989
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Changes of the Ultrastructure and
Distribution after Transient Ischemia and after Reperfusion in the Myocardial Cells of Isolated Perfused Guinea Pig Hearts
Kim, Yong-Mun ; Kim, Ho-Duk ; Rah, Bong-Jin ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 1~18
It has been debated whether postischemic reperfusion is necessarily beneficial to salvage the myocardium after ischemic insult or not. Therefore, this study was undertaken to compare the ultrastructural changes as well as the distribution of
in the ventricular myocardial cells after transient ischemia and after postischemic reperfusion, and to suspect to what extent the postischemic reperfusion is beneficial. After 10 minutes of ischemia, the heart developed wide I bands, glycogen depletion, intramyofibrillar edema, mitochondrial swelling, clumping and migration of chromatin, ghosts of lipid droplets, disintegration of cell junctions, sarcolemmal disruption, and loss of
binding capacity of the sarcolemma and the mitochondria. In spite of reperfusion, in a large number of cells, the ultrastructure was more severely damaged, however,
binding capacity of the sarcolemma and the mitochondria restored. These results suggest that postischemic reperfusion may help the myocardial cells to restore their function to control
to a certain extent, but that it could aggravate the ischemic insult.
Suhplasmalemmal Linear Density -A possible morphologic mesodermal marker-
Kwon, Tae-Jung ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 19~26
Subplamalemmal linear densities (SLD) consist of a thin layer of electron dense material immediately subjacent to the inner leaflet of the palsma membrane. Short segments of extracellualr basal lamina-like material or collagen fibers usually overlie them. SLD has been considered to be a possible morphologic maker of cells of mesodermal origin. The present study confirms the fact that SLD may be of value in the diagnosis of mesodermal or mesenchymal tumor.
Hormonal Activity of Ovarian Fibroma and Thecoma
Lee, Soong-Deok ; Suh, Jin-Suk ; Han, Young-Mee ; Kim, Jung-Ran ; Seo, Jeong-Wook ; Ham, Eui-Keun ; Lee, Hyo-Pyo ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 27~33
Six cases of ovarian fibrous stromal neoplasm were studied clinically, light microscopically and ultrastructurally for the clinico-pathological evidences of hormone production. Of the six cases, two cases were fibroma, three cases were fibrothecoma, and one case was thecoma. Two cases of fibroma and one fibrothecoma were associated with clinical history of menstrual abnormality, however fat staining of the tumor was negative or weakly positive. Two cases of fibrothecoma and one thecoma were negative for the clinical history of hormone imbalance. Fat stain of those cases revealed positive in varying intensity. Ultrastructural examination of fibroma-thecoma group revealed dark and pale cells by their nuclear characteristics. The dark cells had indented nucleus and abundant cytoplasmic organelles of rough ER, Golgi apparatus and mitochondria. Intracytoplasmic cisternal spaces were seen in the dark cell cytoplasm and some lipid droplets were seen around the cisternae. Pale cells had pale swollen nucleus and fine chromatins. Their cytoplasm showed scanty amount of organelles. Fibroma-thecoma spectrum showed varying degree of population of dark cells, light cells and intervening collagenous stroma. Lipid droplet was structurally associated with intracytoplasmic cisterna and they were frequently seen in thecoma and two of the fibrothecoma. But clinical history of hormone imbalance was poorly related to the light microscopic morphology and ultrastructural organization.
A Study of the Pericardial Cell on the Cabbage Butterfly, Pieris rapae L.
Kim, Chang-Whan ; Kim, Woo-Kap ; Lee, Keun-Ok ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 34~48
The oval shaped pericardial cells are clustered along the lateral sides of the heart and irregularly connected with the heart. The cells are bounded by a basement membrane. The basement membranes of the connected two peicardial cells are irregularly linked each other there-fore funnels are formed. The multiple invaginations of the cell membrane are observed and septate junctions develope at the part of enterance of the cell membrane. The coated pits are appeared in the inner side of the invaginated cell membrane. The coated vesicles, tubular and spherical shaped vesicle, Golgi complex containing high electron densed material in the cisternae and mitochondria are observed in the cytoplasm and lysosomes are remarkably well developed. The whirled membrane structures in the multiformed complex bounded by single membrane are linked with low electron densed granules and spherical shaped small granules having high electron density with
in diameter are located between the whirled membrane in a row and gradually secretes the granules and then they produced the multilamellar body. The lysosomal regions of cytoplasm of pericardial cell are appeared negative reaction to the acid phosphatase and according to the results of the electrophoresis, lipoproteins having acid phosphatase activity are contained. The axon is contacted with the pericardial cells.
Ultrastructure of the Ampullate Glands in the Orb Web Spider, Nephila clav ata L. Koch III. Excretory Duct of the Small Ampullate Gland
Moon, Myung-Jin ; Kim, Woo-Kap ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 49~58
Ultrastructure of the excretory duct of the small ampullate gland in the orb web spider, Nephila clavata L. Koch are studied with light and electron microscopes. The small ampullate glands, located near the midline portion of the abdominal cavity, are connected with the spigots(large spinning tubes) on the middle spinnerets and composed of three parts which are the excretory duct, the storage sac and the convoluted tail. The excretory duct of this gland is enclosed by a thin layer of the outer connective tissues. By the morphology of the apical cuticles and internal textures of the epithelial cells, the duct is subdivided into two regions which are proximal duct region near the sac and distal duct region near the spinnerets. At the distal region of the ducts, the subcuticle which had the function of water removal form the progenetive silk material is well developed, whereas at the proximal region this cuticle disappeared and instead of these, endocuticle is developed. Moreover the epithelium of the distal duct region is composed of columnar epithelial cells, but at the proximal region the epithelium is changed to squamous or cuboidal forms. In the cytoplasm of the epithelial cells, rough endoplasmic reticula, Golgi comlexes and large secretory vesicles related to the production of the cuticular materials are well developed. And between the adjacent epithelial cells, specialized septate junction and desmosomes are formed along the plasma membrane.
Ultrastructure of the Ampullate Gland in the Orb Web Spider, Nephila clavata L. Koch IV. Secretory Portion of the Small Ampullate Gland
Moon, Myung-Jin ; Kim, Woo-Kap ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 59~69
Ultrastructure of the secretory portion of the small ampullate gland in the orb web spider, Nephila clavata, has been investigated using the electron microscope. The secretory portion of this gland comprise two different regions which are a S-shaped storage sac and a long, convoluted tail. By the internal textures of the secretory granules, the sac is subdivided into two regions ; the proximal region near the tail and the distal region near the duct. Commonly single layered connectives cover the basal portion of the sac epithelium, and apical portion of the epithelial cells is occupied by the thick cuticles. Within the epithelial cells of both the proximal and distal region, several types of the secretory granules surrounded by a limiting membrane and had characteristic crystalloid are scattered throughout the cytoplasm. The granular size and its electron densities are not coincide with each other according to the maturation level of the granules. The wall of the tail is composed of single layer of columnar epithelial cells, and their nuclei are found at the basal portion of the cells. Dissimilar to the epithelial cells of the sac, apical cuticles are not found at this portion. In the cytoplasm of these cells, numerous secretory granules, synthesized from the rough endoplasmic reticula commonly and had fine fibrous materials, are found. At the cell surface bordering the lumen, microvilli are seen, arid along the cellular boundaries specialized septate junctions and desmosomes appeared.
The Differentiation of the Male Genial Cell in the Storage Sac in Echuroid, Urechis unicinctus : A Fine Structural Study
Jeong, Jin-Wook ; Choe, Rim-Soon ; Shin, Kil-Sang ; Joo, Chung-No ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 70~88
The storage sac of U. unicinctus can be divided into two parts morphologically as well as functionally ; one is proximal and the other distal storage sac. It is because of the seasonal morphological change of the storage sac caused by sperm accumulation in the storage sac. The proximal storage sac contains the mature sperm with the dumbbell-shaped acrosome and well developed one or two mitochondria in the middle piece, whilst the sperm of hemispherical acrosome associate closely with an accessory cell in the distal storage sac. This means that the sperm do not perform the synchronous development in a storage sac, which is not the case of sperm development in the coelomic cytophorus. In addition, the basal membrane of the distal storage sac is different from that of the proximal storage sac in term of tissue formation. Connective tissues, acces-sory cells and small lumen develop on the basal membrane of the distal sto-rage sac, which is well contrasted with the thin basal membrane of the proximal storage sac. The function of the storage sac is discussed in rela-tion of the sperm development and the seasonal change.
Contrast Experiment and Precipitate Analysis in Age-Hardening Aluminium-4% Copper System
Lee, Jeong-Yong ;
Applied Microscopy, volume 19, issue 1, 1989, Pages 89~108