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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 19, Issue 2 - Dec 1989
Volume 19, Issue 1 - May 1989
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Electron Microscopic Observation of Liposomes with Negative Stain
Ryu, Yong-Wun ; Hong, Seong-Woon ; Kim, Tae-Hwan ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 1~16
To investigate the research of the tumor seeker using liposomes with negatively stained. The fine structure and size in liposomes composed of PC, DSPC, DAPC and SM phospholipid were observed. And the yield of vesicle affected with sonication by mechanical force were determined. The results were as follows. 1. The stain of 2% UA are obtained a good resolution from electron microscopic observation to compare the negatively liposomes with PTA, AM and UA solution. 2. The fine structure of liposomes except DAPC, PC and CH alone samples could be observed from EM experiment of liposomes composed of PC, DSPC, DAPC and SM phospholipid. 3. The results of experiment from 10, 20, 40 and 60 times sonication with 30sec, prepared 0.1mM NTA-SM-liposomes were obtained unilamella vesicles from groups of multilamella vesicles of phospholipid. 4. About 50nm diameter liposomes obtained through membration filtration step to prepare homogenized liposomes.
Electron Microscopic Studies on the Morphology and Ultrastructures in Campylobacter jejuni treated with Physico-chemical Disinfectants
Yun, Man-Seok ; Oh, Hak-Shik ; Kim, Chi-Kyung ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 17~26
The cells of Campylobacter jejuni treated with physical or chemical disinfection agents were comparatively examined by scanning and transmission electron microscopies for their morphological features and internal ultrastructures. The normal cells of C. jejuni, showed typical spiral rod shapes. The ribosomes, nucleoids, and other cellular constituents were observed to be distributed evenly throughout the cytoplasm. The cells treated with heat or UV-light were changed to spherical or irregular shapes and their cell envelopes were destroyed to form ghost cells by liberating their cytoplasmic components. The cells treated with chlorine or monochloramine were also changed into irregular round shapes. The chlorinated cells showed very rough surface structures with many blob-like protrusions, while the surface of the monochloramine-treated cells appeared to be relatively smooth.
Ultrastructure on the Forming of the Scale and Socket in the Wing of the Pieris rapae L.
Kang, Seong-Hoon ; Kim, Chang-Shik ; Kim, Woo-Kap ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 27~42
The forming process of scale and socket of Pieris rapae L. during in 30 hr. pupa to in adult was morphologically investigated with scanning electron microscopy and transmission electron microscopy. 1. The scale forming cells which were distinguished from other epidermal cells were first observed in 30 hr. pupa. In the aspect that scale forming cell beared some morphological relations to socket forming cells and in the distribution of its organelles, scale forming cell was divided into three regions-basal region in which nucleus located, neck region which was surrounded by socket forming cells and scale region that was the cytoplasmic projection region over the wing surface. In process of the development of scale forming cell neck region and scale region were extended into the molting space and at this time, the changes of surface structure of scale region have occurred initially. 2. There was a more distinct process that scale region changed into the scale. Scale region which was first originated as clublike projection of the cell body was subsequently elongated and flattened out by broadening of the cytoplasm. After that, in the surface of scale were formed longitudinal ridges and microribs. In the late pupa, the cytoplsam of scale region have autolyzed by lysosome-like bodies and at length, scale which had air spaces, trabecula, pigment granules, longitudinal ridges and transverse ridges. 3. The major protion of socket forming cell located beside neck region of scale forming cell under the wing surface but the processing portion of the cell lay over the wing surface, suggesting that socket forming cells have actively processing. In extending to the molting space of neck and scale region, socket forming cells developed to the molting space and constructed socket.
Effects of Hexavalent Chromium on Mitochondrial Respiration of Rat Kidney and Function of Low-Molecular-Weight, Chromium-Binding Substances
Kang, Soo-Gil ; Boo, Moon-Jong ; Choe, Rim-Soon ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 43~58
To investigate the effects of hexavalent chromium on mitochondrial respiration of rat kidney, various hexavalent chromium concentrations were treated, then respiration and electron transfer enzyme activities were measured. Ultrastructural changes at state IV respiration of mitochondria were also observed. Then, to investigate protective role against hexavalent chromium in the body, low-molecular-weight, chromium-binding substances (LMCr) were purified from livers of rabbits 2hr after intravenously administrated with sodium dichromate at a dose of 74mg per kg body weight. And then, respiration rates of mitochondria treated with LMCr, hexavalent chromium containing 0.7mM chromium were measured. Hexavalent chromium decreased state IV respiration rates and electron transfer enzyme activities of mitochondria, and increased labile membrane and swelling. And partial inhibitions of condensed to orthodox conformational change were observed. Respiration rates of mitochondria treated with LMCr containing 0.7mM chromium did not differ from that of the non-treated mitochondria. But respiration rates of 0.7mM hexavalent chromium-treated mitochondria decreased by 42%, compared to non-treated mitochondria. These results suggest that LMCr may play an important role in detoxification of toxic hexavalent chromium.
Effects of Cadmium and Cadmium Binding Protein on the Respiration of Mitochondria of Rat Kidney
Cho, Hae-Kyoung ; Boo, Moon-Jong ; Choe, Rim-Soon ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 59~73
To investigate the effect of cadmium and cadmium binding protein on the electron transport system and conformational changes of rat kidney mitochondria, various cadmium concentration were treated in vitro and respiration rate, NADH-CoQ reductase activity were measured. Ultrastructural changes at state IV respiration were also observed. CdBP was isolated from the rat liver by Sephadex G-75 column fractionation and treated in vitro with cadmium. Also mitochondrial state IV respiration rate was measured. When cadmium was treated in vitro, state IV respiration and enzyme activity were decreased and ultrastructural transformation of mitochondria from a condensed to an orthodox conformation was inhibited under state IV respiration. In case cadmium and CdBP were treated together, oxygen consumption was more increased than cadmium only. Conformational changes of mitochondria from a condensed to orthodox conformation were also observed. This indicates that CdBP have a protective effect against cadmium toxicity.
An Immunocytochemical Study on Storage Proteins of Ginseng Seed - Tris Buffer Soluble Protein -
Kim, Woo-Kap ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 74~84
Buffer soluble storage proteins of ginseng seed have been localized by electron microscopy using post-embedding immunocytochemical gold labelling technique. Major components of the storage proteins were revealed to be storage protein-1(
, MW 160,000) and storage protein-2(
, MW 70,000). Both of the storage proteins are glycoproteins. Anti-
from rabbit, against
, respectively, reacted on sections of ginseng endosperm tissue embedded in Spurr's epoxy resin. The rabbit antibodies were visualized indirectly by reaction with protein A labelled with colloidal gold. Both storage proteins were found to be accumulated together in the same protein bodies, but their relative contents are not equal.
Morphometric Study of Heart Development in Rat Fetus
Park, Won-Hark ; Lee, Yong-Deok ; Chung, Hyeung-Jae ; Choi, Jeung-Mog ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 85~98
The ventricular myocardia of 14, 16, 18 and 20-day-old rat fetuses and newborns have been studies by light and electron microscopic morphometrics. The volume density of the myocyte and interstitial compartments as well as volume, surface and numerical density of nuclei were estimated by light microscopic morphometrics. Whereas, the volume density of myofibrils and glycogen granules as well as the volume, surface and numerical density of mitochondria were assessed by electron microscopic morphometrics. The volume density of myocyte compartment of the ventricular myocardia in developing fetuses decreased, but increased in newborn rats. On the other hand, the volume density of the interstitial compartment increased in growing fetuses and decreased in newborns. In all groups the volume, surface and numerical density of nuclei decreased gradually with elongation of myocytes. Conversely, the volume, surface and numerical density of mitochondria and volume density of myofibrils and glycogen granules in ventricular myocytes incresed. The increase in numerical density of mitochondria probably reflects an increase in metabolic activity. Sarcomere length also increased during development.
Effects of Adriamycin on Cardiac Ultrastructure and Glutathione-Glutathione Peroxidase System in Mouse
Park, Won-Hark ; Chung, Hyeung-Jae ; Kim, Ssang-Yong ; Lee, Yong-Deok ; Choi, Jeung-Mog ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 99~118
The cardiotoxic effects of acute and chronic administration of adriamycin (ADR) were evaluated in A/J Swiss albino mice. In acute studies, male mice received intravenous ADR, 5mg or 15mg/kg per day for 3 or 1day and were sacrifice 12 hours later. Because the glutathione-glutathione peroxidase system is major pathway for free radical detoxication, glutathione levels and glutathione peroxidase activity was measured. In acute studies, ADR-treated mice exhibited significantly decreased levels(p<0.05) of total glutathione and unchanged levels of oxidized glutathione and percentage of oxidized glutathione. The earliest myocardial fine structural alterations included swelling and degeneration of mitochondria and dilatation of sarcoplasmic reticulum at all dosage of acute models. In chronic studies, mice received 5mg/kg ADR once a week for up to 16 weeks. Levels of total and reduced glutathione were decreased significantly(p<0.01) and oxidized glutathione and percentage of oxidized glutathione were increased significantly (p<0.05). Chronic myocardial lesions included perinuclear vacuolization, seperation of myofibrils and the fasciae adherens of intercalated disc and hypercontraction band within myocyte. Glutathione peroxidase activity reduced significantly (p<0.01) in any group of acute and chronic ADR-treated animals. Test for lipid peroxidation(malondialdehyde) was increased significantly(P<0.01). Thus, we conclude 1) ADR significantly lowers glutathione levels in heart tissue, and 2) cellular damage progress produced by alteration of this system in mouse models of ADR cardiotoxicity. These results suggest that the glutathione-glutathione peroxidase system may be involved in the modulation of ADR-induced cardiotoxicity.
Studies on Transport Mechanisms of Turtle Bladder I . Epithelium of Urinary Bladder
Jeon, Jin-Seok ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 119~137
It has been shown in this and earlier investigation that the turtle bladder mucosa has three main cell types on their mucosal surface. They are the granular cells,
CA cells, and
CA cells. The three major transport mechanisms that occurs in the turtle bladder are sodium reabsorption, proton secretion, and bicarbonate secretion. In the present work the trans-port mechanisms by bladder epithelial cells of freshwater turtle, Pseudemys scripta, are summarized as follows. 1. The granular cells play an important role in sodium transport, while the
CA cells do not appear to play a determining role in sodium transport. 2. It appears that the active sodium transport in the granular cells occurs in two-step process, implying that first, sodium diffuses into the cells, followed by an energy-dependent efflux step, which is catalyzed by the ouabain-sensitive Na-K ATPase. 3. The
type of CA cells are responsible for the proton secretion using the proton pump on the apical plasma membrane, while the
type of CA cells are believed to be responsible for bicarbonate secretion. 4. When looked at under freeze-fracture electron microscopy, the apical plasma membrane of
cells have a characteristic population of rod-shaped intramembranous particles which are believed to be components of the proton pumps. Conversely,
type of CA cells show rod-shaped particles in their basolateral plasma membranes, which is consistent with the proton absorptive, bicarbonate secretory mechanism. 5. In the turtle bladder, the
type of cells are believed to be both responsible for proton transport, but in opposite directions.
The Changes of Electron Microscopic Block Condition According to Seasonal Status
Sohn, Seong-Hyang ; Yoo, Chang-Kyu ; Choe, Rim-Soon ;
Applied Microscopy, volume 19, issue 2, 1989, Pages 138~144
The specimens for electron microscopic observation made in different seasons have different qualities even though they are made by the same procedure. We observed the various specimens made in each four season. As the results, we concluded that the different specimen conditions were caused by the humidity and penetrability of various solution into the block. In spring, fall and winter, the quality of specimen is good and the difference with one another is not found. But in summer, the specimen have worse quality than in other seasons and not good for sectioning process and observation. In summer with high humidity, we can gain better specimens by eliminating the humidity as much as possible in all processes, thus increasing penetration of various solutions into the specimen.