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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 2, Issue 1 - Jun 1972
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Studies on Microstructure of Barium Ferrite. <2nd. Report>
Ahn, Young-Pil ; Oh, Pyoung-Je ; Kim, Dong-Soo ; Doh, Myung-Ki ;
Applied Microscopy, volume 2, issue 1, 1972, Pages 1~6
The Optimum Conditions of Preparing barium Ferrite from
are Sought for with electron microscope. At first to find the optimum sintering temperature, the mixture in 1 : 3 mole ratio. Sintered primarily at
for an hour, is Sintered secondary at
, respectively for an hour. at the optimum temperature,abtained in this way. Sintering time is varied from 10 minutes to 120 minutes with 10 minutes intervals. Through the experiment, It is found that the optimum temperature of
and optimum time of half-one hour.
An Electron Microscopy of Oogenesis and Fertilization in Clonorchis sinensis
Paik, Kyong-Ki ; Kim, Myong-Won ; Choi, Choon-Keun ;
Applied Microscopy, volume 2, issue 1, 1972, Pages 7~15
The morphological and fine structural changes during the oogensis of Clonorchis sinensis were studied on the developing ovums in the ovary and ootype with electron microscope. Adult worms were removed from the hepar of the which and previously infected with metacercariae of Clonorchis sinensis. The ovary including the Mehlis' glands and an ootype from adult worm was prefixed for 1-2 hours in 1.25% glutaraldehyde buffered with 0.2M cacodylate at PH 7.2, secondarily fixed for 30 minutes in potassium bicromate and postfixed for an hour in 1% osmic acid buffered with 0.4M cacodylate at PH 7.2. After fixation tissues were dehydrated in an alcohol series, embedded in Epon 812 from propylene oxide and stained with saturated uranyl acetate and
solution. Material was examined with a Hitachi HS-7S electron microscope. The periphery of the ovary, except for the posterior region, is made up of oogonia. As the oogonia divide they proliferate primary oocytes toward the central part of the ovary. After a period of growth the primary oocyte leaves the ovary and is penetrated by a sperm in the ootype. Sperm penetration immediately activates the primary oocyte to resume its meiotic activity. After the oocytes meiotic activity is completed, the pronuclei fuse to form a single cleavage nucleus which possesses two nucleoli. As the oocytes develop their cytoplamic materials are abundant; small mitochondria are abundant and often their profiles are more unmerous in one part of the cytoplasm than elsewhere; the granular endoplasmic reticulum becomes alveolar-sac form after it leaves the ovary it becomes stratified form. The reticulate Golgi apparatus is apparent in the developed oocyte. A little of cortical granules are distributed inside of the plasma membrane I oogonia and large quantity of cortical granules are arranged just inside of the plasma membrane of the primary oocyte and after fertilization they are disappeared with broken out.
Studies on Eriophyes kuko KISHIDA and its Galls. IV. Studies on the Growing Mite Gall under Light and Electron Microscopes
Kim, Chang-Hyo ; Sigenobu, Kawamatu ; So, In-Yung ;
Applied Microscopy, volume 2, issue 1, 1972, Pages 17~31
Light and electron microscopic studies were made to investigate the morphological changes in growing galls on the leaf of Lycium chinense MILL caused by Eriophyes kuko Kishda. The results are summarized as follows: 1. Light microscopy At the early stage of the invasion of E. kuko on the back side of the young leaf of L. chinense, the.epidermal cells become hypertrophic and develope a gall. As the gall grows, the cells of both palisade and spongy-layers become hypertrophic and these tissues are hard to be distinguished because of their irregular outgrowth. As the gall grows, the nuclei of the gall also become hypertrophic and larger than these of normal cells. 2. Electron microscopy Under electron microscopy the mitochondria, the golgi apparatus and the plastids of the advanced galls are degenerated and disintergrated and the cell walls become thicker than normal ones. The characteristic star bodies and the ring-form structures are found in the mature gall cells.
Electron Microscopic Studies on Microbody of Panax ginseng
Kim, Woop-Kap ; Lee, Bong-Hee ; Oh, Chong-Yon ;
Applied Microscopy, volume 2, issue 1, 1972, Pages 33~37
The distribution, ultrastructure, and cytochemical properties of microbodies (peroxisomes) from the main roots and mature leaves of Panax ginseng were studied by electron microscopy included the activity of catalase in 3, 3'-diaminobenzidine (DAB) medium at pH 9, using glutaraldehyde-fixed tissues. The microbodies, which are about
diameter, were described from mesophyll cells of mature leaves and storage cells and cambial cells of main roots. The microbodies of the ginseng include the coreless, homogeneous matrix, in which catalase activities are present.
Applications of the Scanning Electron Microscope
Kim, Yong-Nak ;
Applied Microscopy, volume 2, issue 1, 1972, Pages 39~46
There are many kinds of microscopes suitable for general studies; optical microscopes(OM), conventional transmission electron microscopes (TEM), and scanning electron microscopes(SEM). The optical microscopes and the conventional transmission electron microscopes are very familiar. The images of these microscopes are directly formed on an image plane with one or more image forming lenses. On the other hand, the image of the scanning electron microscope is formed on a fluorescent screen of a cathode ray tube using a scanning system similar to television technique. In this paper, the features and some applications of the scanning electron microscope will be discussed briefly. The recently available scanning electron microscope, combining a resolution of about
with great depth of field, is favorable when compared to the replica technique. It avoids the problem of specimen damage and the introduction of artifacts. In addition, it permits the examination of many samples that can not be replicated, and provides a broader range of information. The scanning electron microscope has found application in diverse fields of study including biology, chemistry, materials science, semiconductor technology, and many others. In scanning electron microscopy, the secondary electron method. the backscattererd electron method, and the electromotive force method are most widely used, and the transmitted electron method will become more useful. Change-over of magnification can be easily done by controlling the scanning width of the electron probe. It is possible. to continuously vary the magnification over the range from 100 times to 1.00,000 times without readjustment of focusing. Conclusion: With the development of a scanning. electron microscope, it is now possible to observe almost all-information produced through interactions between substances and electrons in the form of image. When the probe is properly focused on the specimen, changing magnification of specimen orientation does not require any change in focus. This is quite different from the conventional transmission electron microscope. It is worthwhile to note that the typical probe currents of
are for below the
of a conventional. transmission microscope. This reduces specimen contamination and specimen damage due to heatings. Outstanding features of the scanning electron microscope include the 'stereoscopic observation of a bulky or fiber specimen in high resolution' and 'observation of potential distribution and electromotive force in semiconductor devices'.