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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 21, Issue 2 - Dec 1991
Volume 21, Issue 1 - May 1991
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Embryological Studies on Somitogenesis of Early Chick Embryos by heat shock and treatments of
-amanitin and cycloheximide
Choe, Rim-Soon ; Park, Yong-Bin ; Kim, Ok-Yong ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 1~13
In order to investigate the factors of the control mechanism of somitogenesis, early chick embryos (H-H stage
) were treated with heat shock,
-amanitin and cycloheximide and morphological changes of somite were examined by light microscopy, transmission and scanning electron microscopy. In normal chick embryo, somites were formed from the somitomere which preexisted in segmental plate. Somites were wrapped with extracellular collagen fibrils and connected with neural tube, notochord and ectoderm. And then, somites were differentiated to sclerotome, dermatome and myotome by the interaction of nervous tissue. Abnormal somites were observed after formation of six or seven so mites in heat shock treated group. Amounts of collagen fibrils were obviously decreased in this group. In cycloheximide treated group, most so mites were smaller and neural tube formation was incomplete. Chromatins were condenced and formed several heterochromatins in the nucleus of somite cells. Lipid like cytoplasmic dense mass and lipid droplets were also observed. Segmentation of somites seemed to be normal progress in
-amanitin treated group. Center of somite, however, hollowed in longitudinal sectioned samples. These results suggested that so mites were already existed in the segmental plate as the form of somitomere. Segmented somites were contacted with neural tube or notochord and the somites were tightly connected with each other by the extracellular collagen fibrils which were secreted from neuroepithelium and somite cells. Somites are thought to differentiate into sclerotome, dermatome and myotome by these interactions.
A Study on the Spermatogenesis and Spermatozoan Fate in the Female Reproductive Tract of the Korean Greater Horseshoe Bat (Rhinolophus ferrumequinum korai)
Kim, Eun-Ju ; Oh, Yung-Keun ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 14~28
The present study was designed in order to clarify ultrastructural characteristies of the spermatogenetic process and to examine survival state of the spermatozoa introduced into the female reproductive tract after autumnal coitus in the Korean greater horseshoe bats(Rhinolophus ferrumeguinum korai). The general morphological characteristics of spermatogenesis were principally similar to those of the other mammalian species; acrosomal formation, flagellar formation, middle piece formation and concentration of the spermatozoan nucleus. The spermatozoa introduced into the vagina were found to be dead forming a vaginal plug, the opaque central core of which consisted of trapped dead spermatozoa. Some spermatozoa introduced into the uterus were observed to be phagocytized by the polymorphonuclear leucocytes infiltrated into the uterine glandular lumen. The oviductal epithelium, consisted of ciliated and secretory cells; the luminal surface of secretory cells were covered by a number of microvilli with well developed glycocalyx, suggesting a close relationship to nutrient (e.g. glycogen) supply for the spermatozoa during hibernation.
Ultrastructure of the Ventral Nephrocytes in the Larva of Lucilia illustris Meigen
Cho, Jeong-Sook ; Kim, Kwan-Seon ; Kim, Woo-Kap ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 29~38
Ventral nephrocytes in the larva of the Lucilia illustris comprise ellipsoid cells situated onto the salivary glands. The cells are
in diameter. Junctional complex beneath the basement membrane hold the plasma membrane in a even contour. Intracellular channels from the juntion complex are well developed at the cortex part of the cell. Coated vesicles pinched off from the channels seems to be connected with the
-vacuoles via the tubular elements, which is regared as selective absorption system from the hemolymph. Two nuclei are sometimes observed in the medulla part of the cell. Ventral nephrocytes contain well-developed rough endoplasmic reticulum and Golgi complex, and numerous mitochondria. These cellular organelles synthesize lysosome. The lysosome not only digest some cell organells but also seems to be related with the
Ultrastructural Study on the Development of Notochordal Cells in Nucleus Pulposus of Human Fetuses
Yoon, Jae-Rhyong ; Bae, Choon-Sang ; Kim, Eun-Kyung ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 39~56
The development of notochordal cells of nucleus pulposus was studied with electron microscope in human fetuses ranging from 30 mm to 260 mm crown-rump length. At 30 mm fetus, primitive notochordal cells were large with central nucleus, few organelles, and their cytoplasm usually contained dense glycogen and fine filaments. Notochordal cells at all ages contained bundles of fine filaments of indeterminate nature. One unusual feature of fetal notochordal cells was the consistent presense of rough endoplasmic reticulum surrounding poorly developed mitochondria. At 50 mm fetus, notochordal cells formed dense masses with interdigitating cell membranes connected by a variety of cell to cell junctions. With increasing age, the cell connections became slender threaded cytoplamic extending from cell and enclosed large extracellular space. Chondrocyte-like cells appeared to be separated by large volumes of extracellular matrix. Viable notochordal and condrocyte-like cells existed in specimen from all age. The extracellular spaces were filled with fibrillar and granular material by 90 mm fetus. Necrotic cells were distinguished by loss of their membrane integrity, vacuolization of their organelles, and the presence of dense osmiophilic masses. In adult tissue, notochordal cells became rounded or irregular in shape and developed a pericellular matrix consisting of collagen fibrile, and dense particle. The structure of notochordal cells and their persistance in the nucleus pulposus after fetal life suggested that they may have a significant role in the formation and maintenance of the nucleus pulposus. The presence of Golgi complex and well-developed endoplasmic reticulum in chondrocyte-like cells suggested that they are capable of producing and maintaining the extracellular matrix.
Microstuctures and Themal Stability of Rapidly Solidified Al-Fe-V-Si-(Mn) Alloys
Kim, Seon-Hwa ; Park, Won-Wook ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 57~66
The main purpose of this paper was to investigate the change of rapidly solidified microstructures and dispersoid behavior according to heat-treatment in the Al-Fe-V-Si-(Mn) alloys. It was found that (111) preferred orientation identified by X-ray diffraction and fine subgrain/large grain were observed in the rapidly solidified Al-Fe-V-Si-(Mn) alloys. Cell boundary of the zone A was composed of the microcrystalline, whereas that of the zone B was amorphous. Decomposition of the Al-Fe-V-Si-(Mn) alloys occurred at about
. These alloys exhibited excellent thermal stability at the elevated temperature. Microstructure of the zone B was more stable than that of the zone A. The spherical dispersoid and 5-fold symmetry phase was also more thermally stable than the amorphous structure of cell boundary.
A TEM Investigation on the Crystal Structure of
Song, Se-Ahn ; Kim, Sung-Hoon ; Suh, Young-Doug ; Kim, Seong-Keun ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 67~75
] molecule, the Buckminsterfullerene, has generated great interest because of its unique molecular structure and of superconductivity exhibited in its alkali-doped solids. We have investigated the molecular stacking and crystal structure of
thin crystals formed on amorphous carbon film. The
powder which was chromatographically purified was dissolved in benzene. The thin crystals of
were observed with a 300 keV transmission electron microscope. Electron diffraction analysis and direct imaging of its molecular stacking were carried out. It was found from this work that the molecules of
diameter are arrayed hexagonally on substrate surface and
lattice planes are quite often found in several types of ED patterns, which can never be explained with a fcc model. Therefore the structure of
thin crystals is hcp, although we cannot fully exclude the possibility of co-existence of hcp and fcc.
Effects of Cadmium Chloride on Fine Structures of Renal Glomerulus and Epithelial Cells of Proximal Convoluted Tubules in Albino Rats
Kwak, Jin-Ku ; Lee, Koon-Za ; Chung, Ho-Sam ; Lee, Kyu-Sik ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 76~95
A single injection of cadmium chloride (3.75 mg/kg) was made into the peritoneal cavities of albino rats. The cortices of kidney were obtained from the experimental animals at 3 hr., 6 hr., 12 hr., 24 hr. and 36 hr. after administration of cadmium chloride, respectively. The specimens of each experimental animal were prefixed in 2% glutaraldehyde-4% paraformaldehyde solution for
hours, and these specimens were post-fixed in 1% osmic acid. After fixation, the specimens were dehydrated with alcohol and acetate and embedded in Epon 812. Ultrathin sections,
thickness were made and stained with uranyl acetate and lead citrate. And all the preparations were observed with Hitachi-600 transmission electron microscope. The results obtained were as follows: 1. The main changes in ultrastructures of the glomeruli observed at 3 hr. after cadmium chloride administration include loss of filtration slit and fenestrae of capillary endothelium that was resulted from thickings of the basal lamina and fusion of pedicels of the podocytes. At 12 hr. after cadmium chloride administration the Bowman's capsules were mostly filled with abnormally thickened and fused pedicels. After 24 hr. however, the only recognized change was loss of fenestrae of the capillary endothelium. And the ultrastructure of the glomeruli were almost normal in 36 hr. after cadmium chloride treatment. 2. At 3 hr. after treatment with cadmium chloride, in the renal tubular cells the vesicles and vacuoles increased in number at the apical portion, of the tubular epithelial cells, the basal infoldings were reduced and the basal lamina was thickened. After 12 hr., a number of phagosomes appeared at the apical portion and the cisternae of rough endoplasmic reticulum were swollen. At 24 hr. after cadmium chloride administration irregularly shaped mitochondria were observed in the apical area, and mitochondria with swollen cristae were found at the basal portion. And after 36 hr. The ultrastructures of the epithelial cells appeared almost normal except for a moderate increase in the number of vesicles and vacuoles. Consequently it is suggested that in albino rats, cadmium chloride induces acute reversible degenerative changes in the glomeruli as well as in the epithelial cells of the proximal convoluted tubules.
Electron Microscopic Study on the Differentiation of the Epidermis of the Albino Rats
Chung, Byung-Hoon ; Lee, Beob-Yi ; Chung, Ho-Sam ; Lee, Kyu-Sik ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 96~116
The authors studied the morphological distinctions of each of the epidermal layers and the time of appearance of the keratohyalin granules and tonofilaments by the processing of development. The skins were obtained from fetal rats at the age of 14th, 16th, 17th, 18th, 19th and 20th day of gestation, of 1st and 3rd day of neonatal life and of 4th week after birth. The specimens were staind with uranyl acetate and lead citrate. The results obtained were as follows. 1. On the 16th-gestation day, the intermediate layer which contained numerous
-glycogen particles was appeared, and hemidesmosomes and desmosome were observed as well. 2. Tonofilaments were first observed on the 17th gestation day. 3. Above-mentioned intermediate layer was differentiated into the granular layer and the spinous layer on the 18th-gestation day. Keratohyaline granules were appeared in association with the ribosomes and the tonofilaments and the compound granules were lipoid granules which were surrounded by ribosomes at the periphery. 4. Ultimately, keratinization began to take place from the 20th-gestation day. At the age of 4th week, the thickeness of epidermis and the amount of keratohyaline granule and tonofibrils were decreased. It is consequently suggested that in the differentiation process of the rat epidermis, keratinization begins after formation of hemidesmosomes and desmosomes, from which the tonofilaments are formed and after keratohyaline granules are formed. Therefore appearance of the keratohyaline granules and formation of the tonofilament appears to have a close relations with the keratinization process of the rat epidermis.
Formation of Dictyosome and Spherosome in Endosperm Cells of Panax ginseng during seed Formation
Yu, Seong-Cheol ; Kim, Woo-Kap ;
Applied Microscopy, volume 21, issue 2, 1991, Pages 117~125
This study has been carried out to investigate the development of dictyosome, and roles of dictyosome about the formation of spherosome in the endosperm cell during seed formation of Panax ginseng with electron microscope. The result is as follows; In the endosperm cells of early stage during seed formation of Panax ginseng, plastid, mitochondria, endoplasmic reticulum, dictyosome and ribosomes are evenly distributed in cytoplasm. Electron lucent vesicles derived from dictyosome are observed in endosperm cells. Vesicles that contain low electron density are derived from forming face of dictyosome and releases into the cytosol. This vesicles formed multi vesicular body or fused with the plasma membrane. The spherical spherosomes are formed from dictyosome containing the lipid materials of even electron density and are gradually increased in size and number. Dictyosome is located in between vacuole and spherosome and it's cisternae form a semicircle and a circle. Some membrane of the protein body that accumulate the storage protein are originate from the spherical vacuole which interfused between vesicles and vacuoles derived from dictyosome.