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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 24, Issue 4 - Dec 1994
Volume 24, Issue 3 - Sep 1994
Volume 24, Issue 2 - Jun 1994
Volume 24, Issue 1 - Mar 1994
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Fine Structure of Book Lung in the Wolf Spider, Pardosa astrigera
Lim, Hyoung-Soo ; Moon, Myung-Jin ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 1~11
The book lung in the wolf spider, Pardosa astrigera was consisted of a series of flattened triangular-shaped air sacs, stacked with about 70 sheets, and was located in the ventrolateral region of opisthosoma. Each hemolymph spaces (average
in thickness) surrounded by the air sacs (average
in thickness). The air sacs was supported by cylindrical cuticular spikes of microfibril bundles. Epithelial cell processes surrounded the hemolymph spaces. The nuclei of the epithelial cells were concentrated near the atrium. In the middle portion of air sac, the epithelial cells formed pillars across the hemolymph spaces and spot desmosome and zonula adherens were seen between the plasma membranes. In the hemolymph space of this spider, granular hemocytes (average diameter
) were the most dominant type of hemocytes. In the medial sinus, the hemolymph flow between the air sacs of a paired book lungs and then flow out of the lung vein. The air comes in the atrium through the ventral lung slit and makes a tidal wave in and out of the air sacs.
Effects of Cyclophosphamide in the Epididymis of the Rat III. Cauda
Cho, Kwang-Phil ; Kim, Jeong-Sang ; Jung, Hae-Man ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 12~25
This research was undertaken to determine the effects of the anticancer and immunosuppressive drug cyclophosphamide (CP) on the epididymis of the male rat in terms of ultrastructural alteration and protein analysis by SDS-PAGE at different groups; control group, 1 week group, 3 weeks group, 5 weeks group were treated with saline (control group) or CP at doses of 20mg/Kg/week, 1 time a week, respectively. In the cytoplasm of the principal cells on the epididymis, the mitochondrial outer and inner membranes were significantly swollen or disrupted. The cisterns of rough endoplasmic reticulum (rER) were also swollen, and a number of Golgi vesicles were increased, respectively. It is suggested that treatment with CP alters the specific cell organelles in all segments of the epididymis. CP caused changes in protein concentrations in cauda of epididymis after CP treatment. Total proteins of 30 to 39 species such as lactate dehydrogenase, carnitine acetyltransferase and acid phosphatase were expressed in the cauda fluid. Then the more CP was increased, the more concentration of proteins caused to decrease, synthesize or increase in epididymal cauda. In contrast to the control group, in particular 29KD and the other 10 proteins in the cauda fluid were decreased or disappeared, respectively, whereas 89KD and the other 6 proteins in the cauda, were increased or synthesized, respectively. The other proteins are not showed distinctive difference. Therefore, it is possible that CP at a high dose accumulation alters epididymal function with dose-related increase or decrease in specific activity of marked proteins for all regions of the epididymis (particularly, specific segment of cauda). These alterations could be mediated by direct, toxic effects of the drug on the epithelium or be secondary to changes in the spermatozoa as a result of the CP treatment.
Effects of Mercuric Chloride on the Differentiation Cerebral Neuron of Chick Embryo ( I )
Kim, Saeng-Gon ; Cho, Kwang-Phil ; Kim, Jeong-Sang ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 26~36
To investigate the effects of mercuric chloride (
) on the differentiation in the cerebral neuron of chick embryo 7 days, the ultrastructural changes in nerve cells injected with a various doses of mercuric chloride were observed with transmission electron microscope. The enzyme activity of the some dehydrogenases, and adenosine triphophate (ATP) were also analyzed. The results obtained are as follows; The ultrastructural changes in 1.0mg-injected group, the nuclear envelope were irregular, and the RER, Golgi complexes and mitochondria were not well developed. In 2.0mg-injected group, the nuclear envelope were partly destroyed or detached, and mitochondria were decreased in number and their cristae were destroyed, too. The RER and Golgi complexes were less developed than those of the normal groups. In general, the activities of dehydrogenases were declined by increasing the dose of mercuric chloride. Lactate dehydrogenase (LDH) activity fatted to below 85% of the normal group in 1.0mg-injected group, and 69% in 2.0mg-injected group. Malate dehydrogenase (MDH) activity was decreased greatly to 76% in 2.0mg-injected group. Succinate dehydrogenase (SDH) activity fatted to 85% in 1.0mg-injected group, and 74% in 2.0mg-injected group. ATP content in 1.0mg-injected group was almost near to the normal level, but it was increased significantly in 2.0mg-injected group.
Electron Microscopic Stain Effect by Tannic acid
Yoon, Chul-Jong ; Han, Joung-Yeon ; Kim, Chul-Woo ; Chi, Je-Geun ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 37~47
Using mouse tissue, we studied electron opacity effect of tannic acid for transmission electron microscopic staining. Tannic acid-glutaraldehyde in 0.1M phosphate buffer was used as a fixative. To compare with this we have tested another method consisting of heavy metal staining after treatment of tannic acid in sodium tetraborate (borax) on glutaraldehyde-fixed sections. We have achieved equally consistent electron opacity in both methods. The elastin, collagen, basal lamina of skin and gap junctions of the epithelial cells gave excellent results, while it was good for glycogen, cilia, and plasma. Also fat cells and lipid droplets gave good preservation when tannic acid was added in the fixative. However, prolonged fixation in tannic acid-added fixative was hazardous for further processing, i.e., sectioning problem and deep electron opacity background.
Ultrastructural Study on the Cerebellar Purkinje Cell of the Head-Irradiated Rat
Ahn, E-Tay ; Yoon, Kyoo-Tae ; Yang, Nam-Gil ; Ko, Jeong-Sik ; Park, Kyung-Ho ; Kim, Jin-Gook ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 48~62
The acute irradiation effect on rat Purkinje cell was carried out. Anesthetized rats, weighing 200-250g each, were exposed their heads to the linear accelerator (ML-4MV) with the doses of 3,000 rads or 6,000 rads respectively. Irradiated rats were sacrificed by perfusion fixation under anesthesia, six hours, two days and six days following the irradiations. Rats were perfused with the fixative of 1% glutaraldehyde-1% paraformaldehyde solution (pH 7.4). Small pieces of cerebellar cortices were taken out. Tissue blocks were washed out, and were refixed in the 2% osmium tetroxide solution. After dehydration, tissues were embedded in the araldite mixture. Ultrathin sections stained with uranyl acetate-lead citrate solution, were examined with an electron microscope. The results observed were as follow; 1. Many dark Purkinje cells exhibited most severe cellular alterations on 6 hours. But after the 2 or 6 days, the cells exhibited only some alterations of cytoplasmic organelles. 2. Many granular and agranular endoplasmic reticula exhibited the fusion of cisterns. These reticular alterations were most severe on 6 hours following irradiation. But the alterations were hardly found on 6 days. 3. In the Golgi region, alterations including the adhesion of lamelliform cisterns, enlarged saccules, and increased number of vesicles, etc, were seen on 6 hours. But the Golgi complexes were almost recovered on 6 days. 4. Lysosomes were abundant on 6 hours or 2 days, but some residual bodies were found on 6 days. 5. Mitochondrial changes were also most severe at on hours, and they were recovered thereafter. From the results, it was concluded that the cerebellar Purkinje cells reacted to the high doses of irradiation by hyperactive protein synthesis, autolytic activities and energy metabolism. The reaction was most active in the early stage. It implies that motor-control function of Purkinje cells are severely disturbed in the early stage of irradiation.
Fine Structure on the Epidermis of the Scalp of the Head-Irradiated Rats
Ko, Jeong-Sik ; Kim, Jae-Ho ; Yang, Nam-Gil ; Ahn, E-Tay ; Park, Kyung-Ho ; Kim, Jin-Gook ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 63~77
This experiment was performed to study the morphological responses of the epidermis of the rat scalp, following X-ray irradiation. Male rats were divided into normal and experimental groups. Rats anesthetized with sodium thiopental, were exposed only on their head areas with a single dose of 3,000rads or 6,000rads, respectively. Radiation was produced by Mitsubishi Linea Accelerator ML-4MV at the speed of 200rads/min. The target distance was 80cm. Animals were sacrificed on six hours, two days and six days following irradiation. By the perfusion fixation through the heart, rats were fixed with 1% glutaraldehyde-1% paraformaldehyde solution. Pieces of the tissue taken from the scalp were refixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by post-fixation with 1% osmium tetroxide, and embedded within araldite mixture. The sections were cut on a LKB-V ultratome, stained with uranyl acetate and lead citrate, and were observed with JEM 100CX-II electron microscope. The results were as follow; 1. Six hours after exposure to 3,000rads of X-ray. Disrupted intercellular spaces, within which some amorphous materials were filled, disrupted mitochondria, and vacuoles in the keratinocytes were frequently observed, but six days after exposure to 3,000rads of X-ray, Morphology of the keratinocytes was generally restored. 2. Many of the morphological changes were seen on the six days after exposure to 6,000rads of X-ray. 3. Widened intercellular spaces and thickened dense plaques of the desmosomes were frequently observed after exposure to 6,000rads of X-ray. 4. In the experimental groups, the Langerhans and the Merkel cells were damaged, similarly to the keratinocyte. Above results suggest that head irradiation with the dose of 3,000rads temporarily damaged the epidermis of the scalp, though most of the structures recover within six days, whereas with the dose of 6,000rads it severely damaged the epidermis without showing any recovering tendency.
Immunoelectron Microscopic Study on the Paneth Cell of Rabbit after the Common Bile Duct Ligation
Park, Kyung-Ho ; Cho, Hwee-Dong ; Yang, Nam-Gil ; Ahn, E-Tay ; Ko, Jeong-Sik ; Kim, Jin-Gook ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 78~92
Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at
. Sections were etched with a saturated solution of sodium m-periodate for 60min. After etching, sections were pretreated with 0.02M tris buffered saline (TBS), pH 8.4, with 1% bovine serum albumin (BSA, Sigma) for 60min, then treated polyclonal rabbit anti-human lysozyme (Dakipatts) diluted 1 : 50 in TBS with 0.1% BSA for 20hr. Subsequently, grids were incubated 60min in biotinylated goat anti rabbit IgG (Amersham) diluted 1 : 100 in TBS with 0.1% BSA. After this, sections were incubated 60min on streptavidin gold G10 (Amersham) diluted 1 : 50 in TBS with 0.1% BSA. After each step, the grids were briefly rinsed with TBS with 0.1% BSA. After the strepavidin gold step, the sections were jet washed with distilled water. Counterstain of the sections performed by uranyl acetate and lead citrate, and observed with JEM 100 CX II electron microscope. Observed results were as follow; 1. Secretory granules of mouse Paneth cells have a lysozyme immunoreactivity and also eosinophil leucocyte of rabbit applied for the positive-control stain, are well labeld with gold particles. 2. Normal rabbit Paneth cells have a lysozyme immunoreactivity restricted on the secretory granules. 3. Amount lysosomes containing myelin figures in the Paneth cells were significantly increased from 5th day after the common bile duct ligation. 4. Immunoreactivity of Paneth cell secretory granules were more activated on the 3rd day after the common bile duct ligation as compared with those of the normal animal. But the lysozyme immunoreactivity were decreased from the 5th day after the common bile duct ligation. 5. Considering the above finding, lysozyme contained Paneth cell are affected following of common bile duct ligation, whereas lysosomes containing myelin-figure do not exhibit any immunoreactive relationship with those of secretory granules.
Hemocyte Types in Adult Grasshpper, Euprepocnemis shirakii Bolivar
Chang, Byung-Soo ; Han, Sung-Sik ; Yoe, Sung-Moon ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 93~104
Six types of hemocytes were identified in adult grasshopper, Euprepocnemis shirakii Bolivar. The morphological and ultrastructural characteristics of these cells were characterized by phase contrast, scanning and transmission electron microscope. The prohemocytes are small and spherical cells with large nucleus. The plasmatocytes are spindle shaped or polymorphic cells which show numerous cytoplasmic processes on the cell surface and they have lysosomes and vesicles that may be involved in phagocytic function. Especially, multivesicular bodies are observed in the polymorphic cells. The granulocytes I are spherical shaped cells. They are characterized by a number of electron dense granules measuring
in average diameter and marginal band of microtubules which are always in close proximity to the cell membrane. The granulocytes II are oval or spindle-shaped cells. They contain large electron dense granules measuring
in average diameter. Their cytoplasm is filled with numerous granules. The spherulocytes contain large amounts of spherules that most of their cytoplasm. Spherules filled with fine granules or flocculent materials. The oenocytoids are large spherical cells with few cytoplasmic organelles. Their cytoplasm contains peculiar aspect of motochondria and numerous polyrobosome.
Comparison of Cytoplasmic Inclusions Induced by Maize Dwarf Mosaic Virus Strain A and B
Choi, Chang-Won ; Gardner, Wayne S. ;
Applied Microscopy, volume 24, issue 2, 1994, Pages 105~114
Comparative ultrastructural studies of sorghum (Sorghum bicolor L. Moench) cultivar (cv.) HOK and cv Pioneer 8680 leaf cells separately infected with maize dwarf mosaic virus (MDMV) strain A and B, respectively, revealed the formation of numerous cylindrical inclusions in both cross and longitudinal sections. The MDMV-A and -B were distinguished by the presence or absence of specific inclusion bodies in the cytoplasm. Electron microscope revealed the presence of pinwheels, bundles, scrolls, and laminated aggregates in Pioneer 8680 sorghum leaf cells infected with MDMV-B while no laminated aggregates were found in cells of HOK sorghum leaf cells infected with MDMV-A. Differences in the ultrastructure of cylindrical inclusions between two strains of MDMV, especially with respect to laminated aggregates, have been morphologically indexed to classify potyviruses into subdivision. The presence of laminated aggregates may be assigned to subdivision III while the absence of laminated aggregates is assigned to subdivision I. These variations of structures associated with cylindrical inclusions appeared virus-specific inductions and may be represented the morphogenesis of inclusion bodies following development of infections.