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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 25, Issue 4 - Dec 1995
Volume 25, Issue 3 - Sep 1995
Volume 25, Issue 2 - Jun 1995
Volume 25, Issue 1 - Mar 1995
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An Electron Microscopic Study on the Main Sensory Trigeminal Nucleus in the Aging Rat Brain
Kim, Myung-Kook ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 1~14
The purpose of this study was to investigate the main sensory trigeminal nucleus in the aging rat brain by means of electron microscope. Male Sprague-Dawley rats, two (control group) and thirty six (aging group) months of age, were used. These animals were sacrificed by perfusion fixation with 2.5% glutaraldehyde-2.0% paraformaldehyde (0.1M phosphate buffer, pH 7.4) under sodium pentobarbital. The objective area was punched out with a sharp-edged metal cylinder of 0.8 mm in diameter. These blocks of tissue were then washed in 0.1M phosphate buffer, postfixed in 2% osmium tetroxide, dehydrated in a graded series of ethyl alcohol, and embedded in Epon 812. Thin sections were cut with Super Nova ultramicrotome, pick up on grids and double stained with lead citrate and uranyl acetate, and observed in JEOL 100B electron microscope. The results were as follows: 1. In the control group, the neuronal cell body of the main sensory trigeminal nucleus was filled with nucleus, Golgi complex, Nissl substance, mitochondria, microfilaments and microtubules. However, few Nissl substances are seen in neuronal cell body. Axoaxonic synapse, axodendritic synapse, axosomatic synapse, axospinous synapse, myelinated and unmyelinated nerve fibers were well organized around cell bodies. Neurons with abnormal changes were not seen. 2. In the aging group, the neuronal cell body of the main sensory trigeminal nucleus contained large number of lipofuscin granules, dense body and swollen mitochondria. Terminal boutons contained glycogen, crystal-like vesicle and membranous indicating first signs of degeneration. The dendrites were found to be in synaptic contact with altered axon terminals. Frequently axons filled with dark axoplasn and splitted myelin sheath were noticed.
Immunocytochemical Localization of Legumin with Developing Stages of Ginseng Endosperm Cells
Lee, Chang-Seob ; Kim, Woo-Kap ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 15~29
Legumin was purified from the endosperm cells of the ginseng seed and analyzed its characteristics. Distributional patterns of the legumin in the endosperm cells were identified using the immunocytochemical method. Legumin was glycoprotein composed of two subunits, molecular weights about 33,000 and 25,000 respectively. The molecular shape of purified legumin stained negatively seems to have hexagonal structure about 10 nm in size. It was localized at the rER, dictyosomes, and in the vacuoles at the early developing stage. Legumin was glycosylated in the dictyosomes and transported from the dictyosomes to the vacuoles. Legumin was accumulated into the central vacuole via the dictyosomes while the endosperm cells were developing. The armorphous proteins containing legumin were scattered randomly within the central vacuoles, which were aggregated together and became gradually spherical shape. Legumin was distributed within the globular protein bodies in the endosperm cells of matured seed. However legumin was not found in the globoids located in the protein bodies.
Ultrastructural Changes in the Adrenal Chromaffin Cells and Ganglion Cells in the Adrenal Gland of Vacor-Induced Diabetic Mongolian Gerbil
Park, Jae-Hwang ; Yoon, Jae-Rhyong ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 30~47
The ultrastructural changes of adrenal chromaffin cells and ganglion cells in the adrenal gland of vacor-induced diabetic Mongolian gerbils were studied by electron microscopy. After one month of vacor-induced diabetes, some chromaffin cells were filled with dense bodies and large cytosomes with formy contents. Most of degenerating axon terminals were observed on chromaffin cells. A few macrophages were found among chromaffin cells at one month after induction. Several of these macrophages were filled with numerous phagosomes. After one month of vacor-induced diabetes, the ganglion cells showed increase in numbers of dense bodies and degenerating dendrites compared with the normal ganglion cells. Both electron dense and lucent types of degenerating axon terminals were found in interstitial space of the ganglion cells. Degenerating unmyelinated and myelinted axons contained dense and lamellar bodies. The satellite cells and macrophages with engulfed degenerated axon terminals were observed. After three months of vacor-induced diabetes, the unmyelinated and myelinated axons showed degenerative changes, whereas no structural changes could be demonstrated in adrenal ganglion and chromaffin cells. The satellite cells and macrophages containing partially digested debris were still commonly observed in the interstitial space of adrenal medulla. These results suggest that the degenerative changes occur in the adrenal ganglion cells as well as adrenal chromaffin cells of vacor-induced diabetic Mongolian gerbils.
Innervation of Neuroepithelial Bodies in Bronchiolar Epithelium of Human Fetal Lung
Min, Yong-Il ; Yoon, Jae-Rhyong ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 48~64
Ultrastructure of nerves and their associated cells in the bronchiolar epithelium of the human fetal lung were studied with ultrastructural and immunohistochemical methods. The neuroendocrine cells were scattered along the basal part of non-ciliated respiratory epithelium and appeared as single cell (solitary neuroendocrine cell) or groups (neuroepithelial bodies). The solitary neuroendocrine cells were devoid of any detectable innervation, while the neuroepithelial bodies were associated with nerve ending containing morphologically afferent (sensory) and efferent (motor) intraepithelial terminals. The afferent nerve endings contained abundant mitochondria with long cristae. The efferent nerve endings were characterized by the presence of synaptic vesicles. Both types of nerve endings formed synaptic junction between nerve endings and neuroepithelial bodies cells. Serial sections of the intraepithelial nerves revealed that both morphologically afferent and efferent types of nerve endings may be formed by the same nerve fiber. By immunohistochemistry, bombesin and serotonin were localized in solitary neuroendocrine cells and neuroepithelial bodies of human fetal lung from various prenatal age groups. These results suggest that the neuroepithelial bodies cells of the human fetal lung have neuroreceptor function.
Ultrastructural Localization of GABAergic Neuronal Components in the Dog Basilar Pons
Lee, Hyun-Sook ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 65~74
An immunocytochemical study of GABA-positive neuronal elements was performed at the electron microscopic level to examine subcellular distribution of the inhibitory neurotransmitter in the dog basilar pons. Electron-dense reaction product was observed in neuronal somata and dendritic processes. One or more unlabeled axon terminals made asymmetric synaptic contacts with these GABAergic somatic and dendritic profiles. A large number of GABA-positive axon terminals were also observed. They made symmetric as well as asymmetric synaptic contacts with unlabeled dendritic profiles. In axo-axonic synapses, postsynaptic axon-like processes were consistently GABA-immunoreactive. These observations suggest that the inhibitory local circuit neurons in the dog basilar pons play a major role in cerebro-ponto-cerebellar circuitry by integrating various afferent inputs and conveying them into the cerebellar cortex and the deep cerebellar nuclei.
Electron Microscopic and Immunohistological Studies on the Ciliated Cells of Rat Oviduct During Estrous Cycle
Song, Suk-Keun ; Song, Yang-Joo ; Chung, Ho-Sam ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 75~85
The goal of the present study is to investigate the precise variation of tubulin substances in the cytoplasm of oviductal ciliated cells and the morphological changes in cytoplasmic organelles of ciliated cells for ciliogenesis by estrous cycles. The animals used in this study were female rat (Sprague Dawley strain), weighing approximately 200 gm. The ampulla oviducts of these animals (at each of estrous cycle) were rapidly excised. At each stage of estrous cycle, the tissues were used for immunocytochemical study and other were used for electron microscopical study. All specimens were observed by the light and electron microscope. The results obtained are as follows: 1. In the ciliated cells at proestrus, Golgi complex showed
stacked cisternae with numerous saccules and vacuoles. Large amount of fibrous granules were located near the Golgi complex. But at metestrus and diestrus, few fibrous granules were seen. 2. A moderate number of rough endoplasmic reticulum and polyribosomes were scattered in the cytoplasm of ciliated cells at proestrus, but were decrease in number at metestrus and diestrus. 3. At proestrus and estrus, there were a large amount of vesicles in the apical cytoplasm of ciliated cells. 4. Numerous mitochondria were located in the apical cytoplasm at proestrus and estrus, but only a few at metestrus and diestrus. 5. At proestrus and estrus, tubulin substances showed strong reactions in the cytoplasm but weak reactions at metestrus and diestrus. It is suggested consequently that the ciliated cells of the rat oviducts showed no morphological changes of cilia but the ultrastructural organelles of the cells were changed in its shape and location during the entire estrous cycle.
Electron Microscopic. Study on Mucous Glands in Frog Skin
Kang, Kyung-Hee ; Jeon, Jin-Seok ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 86~95
This study was accomplished to investigate the ultrastructure of mucous glands in dorsal skin of frog (Rana catesbeiana) by means of scanning and transmission electron microscopes. The dorsal skin of Rana catesbeiana is composed of epidermis and dermis. The cutaneous mucous glands consist of inner glandular epithelial cells and outer myoepithelial cells. Glandular epithelial cells are divided into four types by the microscopic ultrastructure; ER-rich cell, round secretory granule-containing cell, foam-like granule mass-containing cell, mitochondria-rich cell. Myoepithelial cell has a long elliptical nucleus and filled with fibrous materials in the cytoplasm. As a result of scanning microscopic observation, the surface of dorsal skin is covered with cutaneous protrusions. The opening sites of the mucous glands are irregularly distributed in dorsal skin.
Spermiogenesis in the large-footed bat, Myotis macrodactylus
Son, Sung-Won ; Lee, Jung-Hun ; Shin, Hwa-Jeung ; Choi, Byung-Jin ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 96~110
In order to clarify the process of spermiogenesis of the large-footed bat, Myotis macrodactylus, the testis and the epididymis obtained from mature male bats were examined by electron microscope. Based on the variety and diagnostic characters of organells, the spermiogenesis of the large-footed bat. Myotis macrodactylus could be divided into a total of nine phases. The results obtained from the present study are as follows. 1. The spermiogenesis of large-footed bat, Myotis macrodactylus was divided according to the level of fine structural differentiation into five phases, Golgi, cap, acrosome, maturation and spermiation phases, respectively; Golgi, cap, acrosome and spermiation phases were further subdivided into steps of early and late phase respectively and maturation phase has only one step. Hence, the spermiogenesis of the large-footed bat has been divided into a total of nine phases. 2. In the change of chromatin with nucleus, the chromatin granules are condensed in the whole part of nucleus at the late Golgi phase and completed at the maturation phase. 3. The sperm tail in the epididymis consists of nine outer doublets and two central singlet microtubles. Nos. 1, 5, 6, 9 of the outer dense fibers were larger than the others (2, 3, 4, 7, 8).
Ultrastructure of Spermatozoa in Urodeles, Hynobius leechii (Amphibia: Urodela)
Kim, Kgu-Hwan ; Park, Won-Hark ; Lee, Young-Hwan ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 111~121
The ultrastructure of spermatozoa in Hynobius leechii was examined with transmission electron microscope and compared with those of other urodeles and anurans. The perforatorium and the tail show most of the common feature of urodeles. However, there were several ultrastructural characteristics in an acrosome, nucleus, neck, axial rod and mitochondria related to the nucleus. The acrosome was trifoliate in transverse sections and the perforatorium consisted of two different concentric parts with a fine sharp point in a subacrosomal lumen. The nucleus consisted of two different regions of chromatin area and nuclear ridge. The nuclear ridge was composed of several bundles of five to ten minute tubular subunits of 19 nm diameter in this species, while in higher urodeles it was well developed in multi layers. The protoplasmic bead was separated by a cytoplasmic canal except the connection with only the distal portion of the nucleus. The neck was a short cylinder and contained pericentriolar material with transverse striations. In Hynobius the ring was not elongated to the tail and the mitochondria were distributed only in the protoplasmic bead around the nucleus as in Cryptobranchus, while in higher groups of urodeles it elongates to the length of the middle piece and the mitochondria follow the ring. The ring elongation may be related to the distribution of mitochondria. Hynobiidae and Cryptobranchidae are closely related based on structure of neck, the axial rod and location of mitochondria, although they are different in the composition of the axial rod.
Immunoelectron Microscopic Study on the Endocrine Pancreas of the Native Korean Goat: Colocalization of Bovine Pancreatic Polypeptide and Chromogranin
Lee, Jae-Hyun ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 122~129
Pancreatic endocrine cells of the native Korean goat were investigated immunocytochemically at electron microscopic level. All glucagon-, insulin-, somatostatin- and pancreatic polypeptide(PP)-immunoreactive cells were showed chromogranin(CG) immunoreactivity in the secretory granules of each cells. In addition, bovine pancreatic polypeptide immunoreactivity was found to be colocalized in the secretory granules of the glucagon and insulin cells. These observations support that chromogranin is available as the marker of pancreatic endocrine cells on the native Korean goat and BPP colocalized in the secretory granules of glucagon and insulin cells.
TEM specimen preparation for observation of Cu oxides precipitated in the polyimide film and characterization of Cu oxide particles
You, Young-Sek ; Kim, Young-Ho ;
Applied Microscopy, volume 25, issue 1, 1995, Pages 130~138
TEM specimen preparation methods have been examined to characterize Cu oxide particles in the polyimide film. Polyimide films were prepared by coating polyamic acid onto Cu films which had been deposited on TEM-mask and glass substrates and Cu foil, followed by thermal curing. In case of TEM-mask, direct observation was possible without further preparation. In other cases, TEM specimen were made by separating polyimide film from the substrate. Polyimide films were removed from glass and Cu foil by dissolving glass in HF solution and Cu foil in
solution. TEM-mask observation confirms that fine
particles precipitate in the polyimide as a result of reaction of polyamic acid with Cu. However
particle reacts with HF and
solution during dissolving the substrate and interpretation could be misled. It is concluded characterization of
particle in polyimide using TEM-mask is better than other methods.