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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 26, Issue 4 - Dec 1996
Volume 26, Issue 3 - Sep 1996
Volume 26, Issue 2 - Jun 1996
Volume 26, Issue 1 - Mar 1996
Selecting the target year
Effects of Squalene on the Rat Liver Treated with a Anticancer Agent
Kim, Jeong-Sang ; Kim, Jong-Se ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 1~9
This paper aims to probe the effect of SQ in the rat liver which pretreated with CP was examined by transmission electron microscope. In the A group, the difference between the normal and the treated groups were not detected at 24 hours, but the few mitochondria were expanded at the 72 hours. In the B group, the cisternae of rough-surfaced endoplasmic reticulum were partially destructed and attached ribosomes were remarkably decreased at 24 hours. A number of the mitochondria were dilated and increased in number, the filamentous materials also detected at 72 hours. These results suggest that SQ is not only concerned with construction of the membrane of the cell organelles but also decreased the cellular toxicity in the hepatic cells.
An Immuno-Electronmicroscopic Study on the Synoviocytes in the Knee Joint of the Human
Hwang, Douk-Ho ; Chang, Ka-Young ; Lee, Wang-Jae ; Park, Kyung-Han ; Lee, Jong-Bum ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 11~16
This study was designed to observe the ultrastructure of synoviocytes which are concerned with phagocytic function in the knee joint of the human. The synovia were dissected and were fixed for two hours in 0.2% glutaraldehyde and 4% paraformaldehyde solution and processed and finally infused in 2.3 M sucrose and 20% PVP solution. The tissues were cut with the cryoultramicrotome and labelled with primary antibodies (anti-tubulin, anti-vimentin) and secondary antibody-6 nm colloidal gold particles. The tissues were observed under transmission electronmicroscope. The results were followings. 1. In phagocytic synovial cells, the distributions of tubulin were cytoplasm, especially around vacuoles. 2. In phagocytic synovial cells, the distributions of vimentin were cytoplasm. 3. Both tubulin and vimentin were not located inside of vacuoles. On the basis of above findings, it is obvious that the phagocytic functions are concerned with tubulin, and the phagocytic synovial cells contain vimentin.
Fine Structural Aspects of the Venom Production in the Black Widow Spider, Latrodectus mactans
Moon, Myung-Jin ; Tillinghast, Edward K. ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 17~31
The venomous apparatus of the black widow spider, Latrodectus mactans, is composed of chelicera and paired venom glands in the cephalothorax. Each glands is surrounded by a thin adventitia and striated muscular bundles resting on a basal membrane. Along the musculature neuromuscular synaptic contacts are formed by a motor axon and the muscle fibers. The secretory epithelium, which made up of simple and long columnar cells with extensive finger-like processes, creates a simple acinar gland. The secretory surfac is increased by a sort of fringes extended from the basal membrane into the gland lumen, and the luminal surface of the epithelium is marked by the presence of closely spaced microvilli. The venoms of the black widow spider are produced from two types of secretory granules within the epithelial cells. During the secretory phase, these granules are transformed into droplets and suffering a condensation. Finally the secretory products are released by the apocrine secretion. After the gland is emptied, the basal epithelial cells present a high proliferative process and regenerate the columnar epithlial cells.
Ultrastructural Study on the Spermatogenesis of Korean Slug Incilaria fruhstorferi
Chang, Nam-Sub ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 33~45
The spermatogenesis of Korean slug, Incilaria fruhstorferi are observed by electron microscope. The results are as follows: The spermatogenesis of Korean slug, Incilaria fruhstorferi, is processed through the five stages; Spermatogonia, primary spermatocyte, secondary spematocyte, spermatid and spermatozoon. The spermiogenesis, the differentiation of the spermatid, is also processed through the five stages. In stage 1, the numerous and round mitochomdria in the cytoplasm are moved around the nucleus of spermatid. In stage 2, the nucleus of spermatid transformed into the oval shape, and the oval nucleus is surrounded by many rough endoplasmic reticulum. In stage 3, the oval nucleus of spermatid is changed to be curved as an arrow, and then two centrioles appeared behind nucleus. The centriole is sucked into the cytoplasm. and almost all the chromatins are changed into heterochromatins. In stage 4, the nucleus of spermatid are transformed into the oval shape, when the lamella plate chromatin of spermatid form in the nucleoplasm. In stage 5, the oval nucleus is then transformed into the stream-line shape when the lamella plate chromatin of spematid gradually concentrated in the nucleus, and long axoneme (
in length) form from the distal centriole. Two long mitochondria in the middle piece and the main piece of spermatozoon array spirally along a long axoneme, and the mitochondria matrix is gradually filled with electron-dense glycogen particles (
in size). The axoneme of spermatozoon consists of typical 9+2 microtubular pattern.
Electron Microscopic Studies on Cellular Characteristics and Transport Systems in Tight Epithelia
Jeon, Jin-Seok ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 47~57
This study analysed the transport properties of bladder mucosa known as the typical system of 'tight epithelia' by using TEM observation with both rapid freeze-fracture electron microscopy and thin-section method and mainly analysed the cellular characteristics of turtle bladder epithelial cells. The bladder epithelium, like other tight epithelia, consists of a heterogenous population of cells. The majority of the mucosal cells are the granular cells and may function primarily in the process of active
reabsorption in turtle bladder. The remaining two types of cells are rich in mitochondria and is believed to be res-ponsible for a single major transport system, namely,
transport by A-type of cell and urinary
secretion by B-type of cell. As viewed in freeze-fracture electron micrograph, the tight junctions form a continuous tight seal around the epithelial cells, thus restricting diffusion in tight epithelia. In addition, the apical surface membranes have a population of rod-shaped intramembranous particles (IMPs). It is believed that these IMPs probably represent the components of the proton pump. However, it is likely that these characteristics of the apical transporter remain to be clarified in tight epithelial cells.
Morphological Features of the Mouthparts of Silkworm Bombyx mori L. in Relation to the Feeding Responses to Artificial Diet - With Special Reference to Antennae and Maxillae -
Hwang, Seok-Jo ; Mah, Young-Il ; Sohn, Hae-Ryong ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 59~65
The silkworm feeding behaviour reveals two types of feeding response i. e., energetic and inert feeding response to diet. These two new technical terms are being introduced in the present study. The structures of functional organs of mouthpart and antenna of energetic and inert feeding response to diet were examined by scanning electron microscope. There is a big variations in the ultrastructures of different sensilla. The maxillary palpi or sensilla basiconica at the top of maxillary palpus of maxilla are well developed in the energetic larva while in the inert larva, these organs on the right side are extremely deformed. Similarly, these sensilla on the third segment of antenna are well developed in the larva energetic to diet has only two deformed sensilla basiconica, that is why silkworm can not show a proper form. This is related to abnormality and it is due to the fact that behaviour is too strong to select the food but fails and finally dies of the starvation.
Immunoelectron Microscopic Study on the Endocrine Pancreas of the Native Korean Goat
Lee, Jae-Hyun ; Hashimoto, Yoshiharu ; Kon, Yasuhiro ; Sugimura, Makoto ; Lee, Hyeung-Sik ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 67~77
Pancreases obtained from native Korean goats were used, and examined by immunoelectron microscopy using several antisera. Five types cells, glucagon (A), insulin (B), somatostatin (D), and pancreatic polypeptide (PP-I and PP-II) cells, were identified in the pancreatic islets. The morphologies of A, B, and D cells corresponded to the typical charateristics described in previous reports on other mammals. Serotonin immunoreactivity was observed in the D cells on the basis of the granular profiles. Two types of PP cells could be distinguished on the basis of the granular profile: the first type was formed by round, homogeneous secretory granules (
) having a narrow halo between the dense core and limiting membrane, while the other type consisted of cells whose secretory granules (
in the major axis,
in the minor axis) were pleomorphic, having a dense core and a closely fitting limiting membrane. From these results, we suggest that the pancreatic islets of the native Korean goat consist of five types of endocrine cells, A, B, D, PP-I and PP-II cells. Among these, PP-I type cells may correspond to the classical PP of other mammalian pancreases, while PP-II type cells may correspond to the enterochromaffin cells in other species.
The Fine Structure of Human Fetal Nail Matrix
Sohn, Hyung-Sun ; Choi, Jae-Kwon ; Chung, Yun-Young ; Bae, Choon-Sang ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 79~93
The differentiation of nail matrix and fine structure of matrix cells were studied with light and electron microscope using specimens from nails of thumb finger in Korean fetuses 14 to 24 weeks old. Fetal nail matrix consisted of two horizontal layers, thicker ventral and thinner dorsal matrices, originating from invagination of epidermis in proximal nail field. Matrix being generally thicker in its distal region than the apex became gradually thickened with increase of the fetal age. Each matrix consisted of single layer of basal cells and multiple layers of squamous cells which are arranged close to and parallel to the central axis of the nail mairix. The process of keratinization of fetal nail matrix was noted to be occured concurrently in the ventral and dorsal matrices along the central axis of matrix toward distal and dorsal direction. Squamous cells became matured with accumulation of tonofilaments, increase of keratohyalin granules, discharge of membrane coating granules, and narrowing of intercellular spaces, thickening of plasma membrane and finally being transformed into horny cells of nail plate. Horny cells of nail plate filled with fibrous elements in the electron dense amorphous substance. These findings of keratinization process of fetal nail matrix appeared to be similar to those of keratinization in epidermis and inner root sheath of the hair. In the nail matrix, however, corresponding region to the keratogenous zone of growing hair follicle was not observed. Vacuolated squamous cells of nail matrix seen on light microscopy was considered to be artefactual product, but squamous cells with condensed small nuclei rarely found adjacent nail plate was considered to be one of the squamous cells with unknown function. Proximal end of nail plate was observed on dorsal surface of nail field distal to the proximal nail fold at 14 and 16 weeks old human embryos. Proximal prolongation of the proximal end of nail plate was occured with advancing fetal age and afterward 21 weeks nail plate invaded into nail matrix. Melanin granule containing cells and Merkel cells were present only on the basal layer of dorsal nail matirx.
Phase Identification of the Interfacial Reaction Product of
Composite Using Convergent Beam Electron Diffraction Technique
Lee, Jung-Ill ; Lee, Jae-Chul ; Suk, Hyun-Kwang ; Lee, Ho-In ;
Applied Microscopy, volume 26, issue 1, 1996, Pages 95~104
A comprehensive methodology to characterize the interfacial reaction products of
Al composites is introduced on the basis of the experimental results obtained using XRD, SEM and TEM. XRD performed on the electrochemically extracted
Al composite have shown that the interfacial reaction products consist of
having hexagonal crystallographic structure, pure eutectic Si having diamond cubic crystallographic structure, and
, having tetragonal crystalloraphic structure, respectively. According to the images observed by SEM,
, which has been reported to have needle shape, has a hexagonal platelet-shape and eutectic Si is found to have a dendritic shape. In addition eutectic
, was observed to form near interface and/or along the grain boundaries. In order to confirm the results obtained by XRD, the primitive cell volume and reciprocal lattice height of such interfacial reaction products were calculated using the data obtained from convergent beam electron diffraction (CBED) patterns, and then compared with theoretical values.