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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 26, Issue 4 - Dec 1996
Volume 26, Issue 3 - Sep 1996
Volume 26, Issue 2 - Jun 1996
Volume 26, Issue 1 - Mar 1996
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Fine Structure of Blue-green Algae, Microcystis aeruginosa Kutzing
Choi, Min-Kyu ; Kim, Baik-Ho ; Mun, Yeun-Ja ; Chung, Yeun-Tai ; Lee, Jong-Bin ; Wui, In-Sun ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 389~399
In order to understand the morphological differences between two different organic loadings by its upstream, and to compare with other algal groups with references, the fine structure of blue-green algae, Microcystis aeruginosa Kitzing, taken from two branches, Tongbok and Bosung stream of Lake Chuam, Korea pennisula was examined. It showed extinct differences in most physicochemical factors between both branches, except water temperature and pH values. The concentrations of total phosphorus in Tongbok branch were twice as those of Bosung. M. aeruginosa cells were enumerated totally
and these individuals in branch of Tongbok were close to two times as much as Bosung. In light and electron microscopy, natural M. aeruginosa colonies formed irregular shape and non-directional array in amorphous matrix. They were consisted of many kinds of cells, youngs or olds in cell division, solitary, and various size of cells. Each cell ranged from 2.61 to
in diameter, and averaged as
. In cytoplasm, they contained a number of inclusions in various size, shape and appearances. Among them, polyhedral bodies or carboxysomes, a structured granules, photosynthetic lamellae or thylakoids, and gas vacuoles were prominent and easy to recognize. Although it was failed to find the definable morphological variations in the ultrastructure of M. aeruginosa in terms of algal habitual environments, some useful characters were founded, outer layer of cell wall, polyhedral bodies and gas vacuoles, in blue-green algal classification and taxonomy.
Fixation of Cellular Ultrastructure by the Microwave Irradiation
Shin, Kil-Sang ; Kim, Wan-Jong ; Jeon, Jin-Seok ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 401~410
The microwave fixator has recently been introduced in morphological research. The present study was carried out to investigate the ultrastructural effects of microwave fixation of rat brain. kidney, liver and skeletal muscle tissues. The results are as follows: In the case of microwave fixed cerebrum. the cytoplasmic processes of neurons and the various membranous organelles such as nuclear envelope, mitochondria, rough endoplasmic reticulum and Golgi apparatus were well preserved, The myelin sheath wrapping neuronal axon was prominent. Microwave fixed hepatocytes showed the microvilli on the free surface of bile canaliculus, the evident nucleolar components, and typical organelles. In nephron, ultrastructures of glomerulus and Bowman's capsule were preserved, and also tubular wall were structurally observed. Among the skeletal muscle cells, plentiful collagen fibers were appeared, myofibrils and mitochondria were typically observed. In conclusion, the microwave fixation procedures result in an good preservation of the tissues and would be time- and reagent-saving.
Identification of the Zinc-containing Cells in the Cerebellum of Rat by Selenium Method
Cho, Hyun-Wook ; Choe, Eun-Sang ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 411~420
A zinc-specific method (selenium method) has been employed to identify the zinc-containing cells in the cerebellum of the rats. When rats were allowed to survive 24 hours after the sodium selenite administration, zinc selenide reaction products formed in zinc-containing cellular boutons are retrogradely transported to the somata of those boutons. And the zinc selenide products accumulated in somata of the cells can be rendered visible by silver amplification of developer. Zinc-containing cells identified by the method were Bergmann glial and granule cells. Labeled zinc-containing cells were absent in molecular layer and white matter of the cerebellum. In ultrastructural level, the zinc selenide products were located in lysosomes of somata of the zinc-containing cells.
Ultrastructure of Endocrine Cells in the Midgut Epithelium of the Japanese Cockroach, Periplaneta japonica
Yu, Chai-Hyeock ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 421~430
The endocrine cells in the midgut epithelium of the Japanese cockroach, Periplaneta japonica were observed by the light and electron microscopy. The midgut epithelium of the last instar larva and adult cockroach consisted of principal columnar cells, regenerative cells, and secretory granular cells. Midgut endocrine cells were positioned basally as a cone-shaped single cell in the epithelium or underneath the regenerative crypt cells. When midgut epithelium grows and the cell composing it transforms, between the endocrine cells and regenerative cells were made desmosome type junction and large vesicular shaped stretches of loose contact. The endocrine cells were characterized by a clear cytoplasm with abundant Golgi complex and numerous secretory granules. The secretory granules in the cell were spherical and electron dense with their diameter of
. The secretory granules have been observed as discharged by exocytosis on the basal and lateral side of the cell.
Ultrastructural Influence of Nephrotoxic Agents on the Juxtaglomerular Cells of Mice
Park, Kyung-Ho ; Kim, Sang-Chul ; Ahn, E-Tay ; Ko, Jeong-Sik ; Yang, Nam-Gil ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 431~446
This experiment was performed to study the ultrastructural changes of the juxtaglomerular cell of mice following subcutaneous injection of heavy metallic agents. Male mice were divided into normal and experimental groups. The mice were subcutaneouly injected with
(2mg, 5mg or 10 mg/Kg/BW) or with
(5 mg, 10 mg or 20 mg/Kg/BW). Mice were sacrificed on 6 hours, 3 days and 14 days after the injection. Kidneys were fixed in the 2.5% glutaraldehyde-1.5% paraformaldehyde solution, followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture. The sections were cut on a LKB-V ultratome, and ultrathin sections stained with uranyl acetate and lead citrate were observed with JEM 100CX II electron microscope. The results were as follow: 1. Juxtaglomerular cell of the experimental groups showed some alterations, especially in the structures of protein synthesis including dilations and degradations of granular endoplasmic reticula, atrophy of Golgi complex, and numerous free ribosomes in the cytoplasm. 2. Juxtaglomerular cells treated groups showed a number of vacuoles, protogranules and some myelin figures in the cytoplasm, especially in the earlier groups. 3. Juxtaglomerular cells of treated groups, contained a large number of secretory granules showing variable electron densities and pleomorphism in later groups (2 weeks). From the above results, it was concluded that, the mercuric chloride or potassium bichromate induces acute renin release from juxtaglomerular cells of the mice, but many juxtaglomerular cells may secrete prematured secretory granules, or the synthetic system of the cell can not perform normal function.
Fractographic Analysis of Ceramic Composites by Transmission Electron Microscopy using Surface Replication Technique
Jun, Hyeung-Woo ; Kim, Gyeung-Ho ; Kim, Byung-Ho ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 447~456
Fracture surfaces of materials contain useful information ranging from crack path to the mechanism of fracture. Since limitation of electron transparency requires a sample in the form of thin foil for TEM observations, it is impossible to extract such information directly from the fracture surfaces. In this study, the method of surface replication from the ceramic fracture surface is employed to characterize the process of crack propagation in ceramic matrix composites using TEM analysis. The surface replica from the fracture surface in ceramic materials provides detailed surface morphology and more importantly, loosened particles on the fracture surface are collected. Electron diffraction and chemical composition analyses of these particles reveal crack path in the specimen. Furthermore, one can determine the mode of fracture by observing the fracture surface morphology from the image of replica. Two examples are given to illustrate the potential of the surface replication technique. In the first example, apparent toughness increase in
composites at high strain rate is investigated by surface replication to elucidate the mechanism of fracture at different strain rates. The polytypes of SiC formed during the sintering of SiC-AlN composite and their effect on the fracture behavior of SiC-AlN composite are analyzed in the second example.
Water Storage Cells in Succulent Orostachys malacophyllus
Kim, In-Sun ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 457~463
Water storage cells (WSCs) in the leaf succulent Orostachys malacophyllus have been studied to understand their adaptive nature to its coastal habitat employing the electron microscopy. Attention has been paid to the features of vacuoles and plasmodesmata in this study, since leaf tissues in O. malacaphyllus are under continous physiological drought due to its occurrence in the shore-line environment. The WSCs occupied almost all of the leaf volume and appeared empty at low magnifications. Among the WSCs, small rudimentary vascular bundles were scattered throughout the internal volume. However, in high magnification the WSCs were vacuolate in most cases and vacuolization into a well-developed huge central vacuole was very common phenomenon. Such vacuolization has been detected within the vacuoles as well as within the cytoplasms. Well-developed plasmodesmata were often found in cells appeared to be mucilagenous. Moreover, plasmodesmata being involved in the secretion of materials or structures were even encountered. Thus, vacuolization from various sizes of vacuoles in the WSCs to have a huge central vacuole seems playing an important role in adapting the plant itself to its coastal habitat.
Some Observations on the Fine Structure of Vacuolar Apparatus Affected by Dehydrocholic acid, Cholesterol and Phosphatidylcholine
Shin, Young-Chul ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 465~477
The influence of dehydrocholic acid, cholesterol and phosphstidylcholie to the fine structure of vacuolar apparatus was investigated to better understand the mechanism of intracellular transport of bile constituents in the hepatocytes of rats. The cis Golgi cisterns faced toward the bile canaliculi both in normal and experimental groups. In the hepatocytes from the rats of experimental groups, the primary organic solutes in bile influence the Gogi apparatus, ER and lysosome in the way of increase, cisternal dilation or budding to form the vacuoles. In the dehydrocholic acid group, the cis Golgi cisterns appeared to be sacculated and showed buds, which were probably separated to be vacuoles. Some of the vacuoles appeared to be fused to the bile canaliculi. In the cholesterol and phosphatidylcholine groups, the Golgi cisterns appeared to be dilated and lysosomes were increased in the vicinity of bile canaliculi. The cis Golgi cisterns showing linear saccular fashions were occasonally observed. The increase of lysosomes were more predominant in the cholesterol group. The evidence suggests that dehydrocholic acid is mainly transported through the ER and cis Golgi cisterns, and cholesterol and phosphatidylcholine are mainly transported through the ER and lysosomes via the trans Golgi cisterns, but the cholesterols are frequently transported via the lysosomes.
Computer Simulations of HRTEM Images in GaAs/AlAs/InGaAs Epilayers
Lee, Hwack-Joo ; Ryu, Hyun ; Lee, J.D. ; Nahm, Sahn ;
Applied Microscopy, volume 26, issue 4, 1996, Pages 479~487
Thin epilayer structures of GaAs/AlAs/InGaAs, grown by Molecular Beam Epitaxy, were investigated by high resolution transmission electron microscopy, Image in the  zone axis was taken and compared with the calculated images. The supercell structure which contains GaAs, AlAs and InGaAs layers was designed and was employed in the image calculation with MacTempas computer program. Good agreement was shown between experimental image and a set of calculated images with varying defocus and sample thickness.