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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 27, Issue 4 - Dec 1997
Volume 27, Issue 3 - Sep 1997
Volume 27, Issue 2 - Jun 1997
Volume 27, Issue 1 - Mar 1997
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Silver Impregnation and Electron Microscopic Studies on the Synapse in the Visual Cortex of Rat during Postnatal Development
Lee, Hee-Lai ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 347~355
These studies were performed to observe the morphological changes of synapses in the visual cortex of rat during early postnatal development. Specimens of the visual cortex were taken from rats (Sprague Dawley) at 1, 3, 7, 14 and 21 days of age, and prepared for silver impregnation and electron microscopy. The number of synapse and the length of postsynaptic thickening were increased progressively with age, especially 14 and 21 days. The number of dendritic spine was increased conspicuously on postnatal days 14-21. And asymmetic, curved and axo-spinous synapses were increased markedly at the same ages. The present findings suggest that spurt of synaptogenesis in the rat visual cortex occurs during early postnatal development, especially in second to 3rd week period and asymmetric and/or curved axo-spinous synapse is a matured form of synapse with advanced age.
Ultrastructural Changes of the Bruch's Membrane and the Pigment Epithelial Cells of the Mouse Retina with Age
Ko, Jeong-Sik ; Park, Byung-Lok ; Ahn, E-Tay ; Park, Kyung-Ho ; Kim, Jin-Gook ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 357~372
To study the age-related morphological differences of the retinal pigment cells and Bruch's membrane of mouse, retinae of one week-old, five weeks-old, eight weeks-old, six months-old, twelve months-old, eighteen months-old, twenty-four months-old and thirty months-old ICR mice were dissected out under anesthesia. Pieces of the tissue taken from the posterior region of the retina were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1 M Millonig's phosphate buffer, pH 7.3), and 1% osmium tetroxide (0.1 M Millonig's phosphate buffer, pH 7.3), and embedded in araldite mixture. The ultrathin sections were stained with uranyl acetate and lead citrate, and were observed under a JEM 100 CX-II electron microscope. Observed results were as follows: 1. Retinae of one week old mouse exhibit that some parts of the pigment cell provided with basal foldings, whereas other parts of the one contain without basal foldings. After (ive weeks-old, all retinal pigment cells have the basal infoldings. 2. In the one week-old, stage 1 and stage 2 melanosomes were observed in the retinal pigments cells, but after five weeks-old, most of the retinal pigment cells contain some matured stage melanosomes (stage III and stage IV). 3. The phagosomes in the retinal pigment cells were increased during aging. 4. After eighteen months-old, electron dense materials are observed within the basal infoldings. 5. After eighteen months-old, the thickness of the Bruch's membrane is prominently increased. The thickness of the basal laminae of the pigment cell and the choriocapillary endothelium is more prominently increased as compared with that of the other components of the Bruch's membrane. 6. The thickness of the basal lamina of the pigment cell is more prominently increased as compared with that of the choriocapillary endothelium on aging. From the above results, it was suggested that the pigment cell and Bruch's membrane matures structurally In five weeks, and the function of the pigment cell is prominently suppressed around eighteen months-old, and thereafter the functional suppression is continued on aging.
Ultrastructural Changes of the Renal Corpuscle of the Mouse with Age
Ko, Jeong-Sik ; Park, Sook-Hyun ; Ahn, E-Tay ; Park, Kyung-Ho ; Kim, Jin-Gook ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 373~389
Morphological difference of the renal glomerulus at different age groups have been studies in one week-old, five weeks-old, eight weeks-old, six months-old, twelve months-old, eighteen months-old, twenty-four months-old, and thirty months-old ICR mice. Pieces of the tissue taken from the renal corticies were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solution (0.1 Millonig's phosphate buffer pH 7.3), and 1% osmium tetroxide solution (0.1 M Millonig's phosphate buffer, pH 7.3), and were embedded in araldite mixture. The ultrathin sections were stained with uranyl acetate and lead citrate solution, and were observed under a JEM 100CX-II electron microscope. The results were as follows: 1 In the one week-old mouse, thicknesses of the three layers of the glomeluar basal lamina (lamina densa, lamina rara interna and lamina rara externa) are similar, but in the five weeks-old mouse, thick lamina densa becomes a greater portion of the thickness of whole glomerular basal lamina. 2. No difference was noticed between thickness of the renal glomerular basal lamina of the five weeks-old mouse compare with that of the one week-old one, but basal lamina of the eight weeks-old one is thickened considerably and thicknesses were maintained through twelve months-old one. After eighteen months, the thickness of the glomerular basal lamina is increased remarkably. 3. After eighteen months, electron dense deposits within the basal lamina of the renal glomeruli are observed frequently. 4. Amount of the microfilaments in the mesangial cells and the mesangial matrices are increasing during aging. 5. The thicknesses of the basal laminae of the Bowman's capsule are increasing during aging. 6. After twenty four months, the proximal tubular cell-like parietal cells with well developed microvilli are observed frequently. From the above results, it was suggested that the renal glomerulus matures structurally in five weeks, and the function of the glomerulus is suppressed after eighteen months.
Ultrastructural Characteristics of Glomeruli in EGS/Kist Mice Showing High Proteinuria
Lee, Chul-Ho ; Hyun, Byung-Hwa ; Jeong, Young-Gil ; Kim, Moo-Kang ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 391~402
Using the electron microscopical method, this study was attempted to investigate the ultrastructural characteristics of glomeruli in FGS/Kist mice showing high proteinuria. The mucus-like substances were observed in glomerular capillary lumen which were usually narrowed by the widening of mesangial area filled with electron-dense deposits. Some portions of basal lamina in capillary wall were projecting to urinary space. Moreover, many vacuoles were observed in the cytoplasm of visceral epithelial cells, and an irregular-shaped fusion or effacement of foot processes were often shown in areas adjacent to basal lamina of glomeruli.
The Localization of the Specific Antigenic Protein in the Tissue of Paragonimus westermani Metacercaria
Kim, Soo-Jin ; Roh, Tae-Hoon ; Joo, Kyoung-Hwan ; Rim, Han-Jong ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 403~416
In order to observe the localization of the specific antigenic protein in the tissue of Paragonimus westermani metacercaria, immunogoldlabeling method was applied using IgG of the dog which were infected with Paragonimus westermani metacercaria and IgG of rabbits which were immunized with purified 23 kDa protein from metacercaria of the Paragenimus westermani. The metacercaria worm tissues obtained from Cambaroides similis were embedded in Lowicryl HM20 medium, treated with infected and immunized IgG and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the tissue antigen of Paragonimus westermani metacercaria, the content of excretory bladder which was highly dense electron density was constituted in the excretory bladder of the parenchymal tissue. In the metacercaria tissues antigen reacted with IgG of infected dog. Labeled gold particles distributed on the interstitial matrix of parenchymal cells, fibrous granules of parenchymal tissue and the content of excretory bladder. High antigenicity was observed on content of excretory bladder. It was found to be specifically distributed at the tissue of Paragonimus westermani metacercaria. In the tissues antigen reacted with IgG of immunized rabbit. Labeled gold particles randomly distributed on the interstitial matrix and fibrous granules of parenchymal tissue but in the content of excretory bladder of Paragonimus westermani metacercaria, gold particles were richly labeled. Therefore, the 23 kDa protein contained with Paragonimus westermani metacercaria was found protein which was specifically constituted at the content of excretory bladder of Paragonimum westermani metacercaria. The 23 kDa protein was commonly contained from of Paragonimus westermani metacercaria to adult and showed strong antigenicity against the immunized and infected IgG.
Comparative Ultrastructures of the Fertilized Egg Envelopes in Golden severum, Convic cichlid and Discus, Cichlidae, Teleost
Deung, Young-Kun ; Reu, Dong-Suck ; Kim, Dong-Heui ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 417~432
The structures of the fertilized egg envelope in three species of cichlidae, golden severum (Cichlasoma severum var.), convic cichlid (Cichlasoma nigrofasciatum) and discus (Symphysodon aequifasciatus) were investigated by routine light and electron microscopies. The fertilized eggs of all three species were of the non-transparent, ellipsoidal, adhesive and non-floted type. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. In golden severum, an outer surface of egg envelope was covered by a reticular layer, that of convic cichlid was covered by an amorphous reticular layer and that of discus was covered by a branched reticular layer. The fertilized egg envelopes consisted of two distinct layers, an adhesive outer layer and an inner layer, consisted of lamellae alternating with interlamellae of lower electron density, in all three species; an inner layer of golden severum was
layers, that of convic cichlid was
layers, and that of discus was
Morphological Study on the Changes in the Kidney of the Water-deprived Mongolian gerbil (Meriones unguiculatus)
Kim, Moo-Kang ; Lee, Keun-Jwa ; Jeong, Young-Gil ; Song, Chi-Won ; Lee, Kyeng-Youl ; Park, Il-Koun ; Lee, Chul-Ho ; Lee, Ki-Houn ; Hyun, Byung-Hwa ; Kim, Gil-Soo ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 433~452
Mongolian gerbil (Meriones unguiculatus) has been as an animal model for studing the neurological diseases such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animals. In order to accomplish the this research, first of all another divided the laboratory animals 5 groups of which each group include the 5 animals. In this study were investigated the histological structure in the kidney, measured the plasma osmolalities at the time of sacrifice of indivisual animals, and the body weights every day during water-deprived. The results obtained in this study were summarized as followings: 1. The body weights and decreasing rates of the body weight in water-deprived mongolian gerbil groups were continuosly decreased. 2. The plasma osmolalities were increased from the 5th water-deprived day, after then the gradually increasing reached nearly its equilibrium state at the 10th water-deprived day. 3. The urine volumes were abruptly decreased from the 2th water-deprived day, after then the gradually decreasing patterns were reached nearly its equilibrium state at the 10th day, and stopped the 11th day. 4. In the light microscopical observation of the kidney, glomerular capillary loop thickening, mesangial matrix increasing, sclerosis, glomerular cystic atrophy, interstitial fibrosis, tubular dilatation, mononuclear interstitial inflammation, interstitial mineralization, and hyperplasia of the collecting duct epithelium in the cortex area, were observed from the 10th water deprived day, and the lesions were gradually severe changed as the time lapse. 5. In the electron microscopical findings of the kidney, the degenerative changes of endothelial cell, podocyte and mesangial cell in glomeruli were initially observed on the 10th water-deprived day as well as the degeneration of microvilli and intracellular organelle in the renal tubules.
The Tissue Types of Stroma in Some Species of Hypoxylon
Lee, Yang-Soo ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 453~460
The tissue types of stromata were observed intensively in four species of Hypoxylon under a scanning electron microscope (SEM). These stromata were sectioned with a freezing fracture method for observation. Several tissue types were recognized and stable in each species. This study presents the most intensive observation of tissue types of each layer of stromata. It will be useful for taxonomic criteria for the species level. However, the tissue types can not be major taxonomical criteria for the genus Hypoxylon.
Distribution and Ultrastructure of Dopaminergic Neurons in the Substantia Nigra of Mongolian Gerbil (Meriones unguiculates)
Choi, Wol-Bong ; Yoon, Sang-Seon ; Ko, Byoung-Moon ; Jo, Seung-Mook ; Nam, Seong-Ahn ; Choi, Chang-Do ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 461~472
The substantia nigra of the Mongolian gerbil was studies by tyrosine hydroxylase immunohistochemistry and immunoelectron microscopy with preembedding method. The purpose was to obtain information on the distribution and ultrastructure of the Tyrosine hydroxylase immunoreactive and dopaminergic neurons in the substantia nigra, in order to provide the necessary background for the gerbil. Large number of tyrosine hydroxylase immunoreactive neurons were located in the compact part of substantia nigra. Findings in the gerbil, compared to observations in the other species, included the presence of prominent bundles of tyrosine hydroxylase immunoreactive cytoplasmic processes passing in the dorsoventral direction from pars compacta into pars reticulata at middle and caudal levels of the substantia nigra, and the presence of a distinct tyrosine hydroxylase immunoreactive substantia nigra pars lateralis. Tyrosine hydroxylase immunoreactive neurons had well-developed cell organelles, especially rough endoplasmic reticulum, free ribosome and poly-ribosome, and showed the infoldings of the nuclear envelope. We anticipate that the present description of the cellular organization of the tyrosine hydroxylase immunoreactive dopaminergic area in the substantia nigra of gerbil will be useful for the animal experimental model of Parkinson's disease.
Diffraction Characteristics of Mechanically Alloyed Nanocrystalline FeAl
Choi, Keun-Seob ; Kim, Do-Hyang ; Hong, Kyung-Tae ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 473~481
Disorder-order transformation of nanocrystalline FeAl have been investigated by a combination of electron and X-ray diffraction analysis including high resolution electron microscopy and differential scanning calorimetry. Fe-50at.%Al powders mechanically alloyed for 90 hours consist of
nm size grains haying either disordered b.c.c. structure or amorphous structure. X-ray and electron diffraction of mechanically alloyed FeAl powders show that disorder-order transformation occurs at the temperature range of
. Such a low-temperature ordering behavior exhibiting an exothermic reaction is attributable to the nm-scale grain structure with a large amount of defects accumulated during mechanical alloying process.
A Study on Misfit Dislocation Generation in InAs Epilayers Grown on InP Substrates by Metalorganic Chemical-Vapor Deposition
Kim, Jwa-Yeon ; Yun, Eui-Jung ; Park, Kyeong-Soon ;
Applied Microscopy, volume 27, issue 4, 1997, Pages 483~488
A misfit dislocation generation in InAs epilayers grown on (001) InP substrates (oriented
off (001) toward the  direction) using metalorganic chemical-vapor deposition was studied. The InAs film of 17 nm thickness grown at
showed the three different arrays of dislocations: a straight orthogonal array to the <110> direction, an array to the >100> direction, and an array tilted by a degree of
from the  direction. All of the dislocations had a/2<101> Burgers vectors inclined
to the interface. Upon annealing at
the InAs films with 60, 140 and 220 nm thicknesses, most of the misfit dislocations became the Lomer type
oriented exactly along the >110> direction. These misfit dislocation spacings were decreased with increasing the InAs thickness up to 220 nm thickness. This phenomena was interpreted by the relationship between the dislocation interaction energy among parallel misfit dislocations and the opposite remnant InAs epilayer strain energy. The distance between misfit dislocations was measured by transmission electron microscopy.