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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 28, Issue 4 - Dec 1998
Volume 28, Issue 3 - Sep 1998
Volume 28, Issue 2 - Jun 1998
Volume 28, Issue 1 - Mar 1998
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Morphologic Study on the Changes of Skin Structure of Hairless Mouse by Ultraviolet Irradiation
Song, Sun-Young ; Yoon, Jung-Sik ; Chung, Min-Ju ; Chung, Kyung-A ; Roh, Young-Bok ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 127~137
This study was observed of the skin that changed after irradiation of the ultraviolet A. All the mouse were hairless which the weight are about 25g and the ages
weeks old. The mouse were divided into six groups; control, irradiated for 6 hours, 3 days, 7 days, 14 days, 21 days and 28 days. Each group was irradiated with ultraviolet that is
of wavelengths. After irradiated, the skin was observed with the electron microscope and the light microscope. The results are as follow: 1) Light microscopy With following irradiation, the epidermis was not changed to most groups but at the 28 days group was thickened and deposit the melanocyte. The elastic fibers within the epidermis were thickened and twisted with following irradiation. 2) Eelectron microscopy The elastic fibers were slightly clumped at 6 hours group, mildly increased and partly aggregated in the 3 days group, branched and tangled at 7 days group, irregulated and electron density at 14 days group, sightly thickened and twisted at 21 days group, and randomly arranged, shortened, twisted, and electron density at 28 days group.
Ultrastructural Study on Development of the Superior Cervical Ganglion of Human Fetuses
Kim, Dae-Young ; Kim, Baik-Yoon ; Yoon, Jae-Rhyong ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 139~158
The development of the superior cervical ganglion was studied by electron microscopic method in human fetuses ranging from 40 mm to 260 mm of crown-rump length (10 to 30 weeks of gestational age). At 40 mm fetus, the superior cervical ganglion was composed of clusters of undifferentiated cell, primitive neuroblast, primitive supporting cell, and unmyelinated fibers. At 70mm fetus, the neuroblasts and their processes were ensheated by the bodies or processes of satellite cells. The cytoplasm of the neuroblast contained rough endoplasmic reticulum, mitochondria, Golgi complex, Nissl bodies and dense-cored vesicles. As the neuroblasts grew and differentiated dense-cored vesicles moved away from perikaryal cytoplasm into developing processes. Synaptic contacts between the cholinergic axon and dendrites of postganglionic neuron and a few axosomatic synapses were first observed at 70 mm fetus. At 90 mm fetus the superior cervical ganglion consisted of neuroblasts, satellite cells, granule-containing cells, and unmyelinated nerve fibers. The ganglion cells increased somewhat in numbers and size by 150 mm fetus. Further differentiation resulted in the formation of young ganglion cells, whose cytoplasm was densely filled with cell organelles. During next prenatal stage up to 260 mm fetus, the cytoplasm of the ganglion cells contained except for large pigment granules, all intracytoplasmic structures which were also found in mature superior cervical ganglion. A great number of synaptic contact zones between the cholinergic preganglionic axon and the dendrites of the postganglionic neuron were observed and a few axosomatic synapses were also observed. Two morphological types of the granule-containing cells in the superior cervical ganglion were first identified at 90 mm fetus. Type I granule-containing cell occurred in solitary, whereas type II tended to appeared in clusters near the blood capillaries. Synaptic contacts were first found on the solitary granule-containing cell at 150 mm fetus. Synaptic contacts between the soma of type I granule-containing cells and preganglionic axon termials were observed. In addition, synaptic junctions between the processes of the granule- containing cells and dendrites of postganglionic neuron were also observed from 150 mm fetus onward. In conclusion, superior cervical ganglion cells and granule-containing cells arise from a common undifferentiated cell precursor of neural crest . The granule-containg cells exhibit a local modulatory feedback system in the superior cervical ganglion and nay serve as interneurons between the preganglionic and postganglionic cells.
Ultrastructure of Capitate Glandular Trichome in Leaf of Thymus quinquecostatus
Shin, Hyun-Chur ; Yu, Seong-Cheol ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 159~170
The glandular secretory system of the capitate gandular trichomes in leaf of Thymus quinquecostatus Celakovsky was examined by transmission electron microscope. The glandular trichome was consisted of three cell layers; an basal cell layer, a stalk cell with single-celled intermediate layer and a discoid secretory layer with thickened cuticle. The secretory cell was dense, rich in mitochondria, rER, plastds, Golgi complex and had many vesicular structure. Typical plastids with reticulate body and plastoglobule were present in glandular trichome. The tytoplasm of secretory cell was filled with osmiophilic secretory materials. The secretory vesicles, originated from Golgi complex, appeared as membrane bounded vesicles and secreted to the outer wall surface. The presences of well developed rER, mitochondria, Golgi complex, and membrane-bounded vesicles fused with plasmalemma in the secreting cells indicate that the granulocrine mechanism of secretion was occurring in T. quinquecostatus. Subcuticular cavity was developed between the cuticular layer and the secretory cell wall, and it formed above the secretory cell upon separation of cuticle-wall.
A Cytochemical Study on the Vacuolar Apparatus Participating in the Transport of Bile Acids in the Rat Hepatocytes (Cytochemical Study on the Vacuolar Apparatus for Bile Acid Transport)
Shin, Young-Chul ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 171~180
In the present study, the vacuolar apparatus were investigated in the hepatocytes of rats treated with DA by transmission electron microscopy of conventional and cytochemical thin sections. In the rats after 20 min of dehydrocholic acid treatment, the cis Golgj cisterns were sacculated in line. The saccule occasionally occured by elongation and attenuated neck. The lysosomes also showed protrudent saccule. The vesicles were observed near the cis Golgi cisterns, lysosome and bile canaliculi. Some of the vesicles appeared to be fused to bile canaliculi. The cis Golgi cisterns usually faced toward the bile canaliculi both in normal and experimental groups. The cis Golgi cisterns, protrudent saccule and vesicles were almost devoid of visible contents. The osmium deposits were heavy on the protrudent saccule as well as on the cis Golgi cisterns or on the vesicles isolated near by, but they were light or not observed on the vesicles in the immediate vicinity of bile canaliculi. The acid phosphatase activities appeared on the lysosome and vesicles located near by, but did not appear on the vesicles as approaching closer to the bile canaliculi. The evidence suggests that the vesicles are derived from the cis Gogi cistern and lysosomes and fuse to bile canaliculi for exocytosis, and that the activity in the vesicles is diminished as approaching closer to the bile canaliculi.
Ultrastructural Study on the Spermatogenesis of Pale Chub (Zacco platypus)
Cho, Jang-Hyeon ; Reu, Dong-Suck ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 181~191
The spermatogenesis of pale chub (Zacco platypus) was investigated morphologically. The testis of pale chub contained numerous testicular sacs. These testicular sacs were bounded on neighboring sacs by single layer of squamous cells. Also, differentiated sperms were filled in the sacs. In the stage of spermatogonium, the germ cells had a large nucleus and a distinct nucleolus, and mitochondrial development was prominent. In the primary and secondary spermatogonia, these cells had a round electron-dense nucleus, reduced cytoplasm, and mitochondria were congregated in the side of cytoplasm. The highly condensed chromatin of sperms was electron-dense, the acrosome was not found in the head of the sperm and a motile flagellum consisted of an axoneme with a typical 9+2 pattern of microtubules.
The Fine Structure of the Sperm Ball and Sperm of Urechis unicinctus and Immunogold Localization of
Shin, Kil-Sang ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 193~205
The Urechis unicinctus sperm and spermatogenic cells prepared from the testis are investigated to identify
of axoneme microtubules using mouse monoclonal
as the first Ab and Gold(10nm) conjugated goat anti-mouse IgG as the Ab marker. The Ag-Ab reaction analyzed excellently the localization of
and the gold particles incorporated with the proximal and distal centrioles, manchette microtubules, and flagellum. The gold particles can be also observed in the spermatogenic cells while the cells are still in sperm ball which is composed of a somatic cell and spermatogenic cells. The sperm ball is the functional unit of sperm production in U unicinctus testis. The spermatids are developed from the spermatogenic cells in the sperm ball and released into the testis cavity through a cortical cytoplasmic opening. The spermatid architectures are similar with the mature sperm of the testis cavity in aspects of shape of discoid acrosome, degree of nuclear condensation and ring type of mitochondrion. However, the distal centriole connecting with the flagella can be observed from the mature sperm while the both proximal and distal centrioles reveal only in the spermatids. The proximal centriole is directly connected with nuclear outer membrane during the stage of nuclear condensation and oriented perpendicularly to the distal centriole whose axis coinciding with the longitudinal axis of the spermatozoon. There are indications that the distal centriole is intimately associated with the polymerization of the flagellum. The manchette microtubules appear during spermatid development but the mature sperm have round head and no conspicuous middle piece.
Ultrastructural Studies on the Adrenal Cortex of The Developmental Rats
Park, Joo-Hee ; Lee, Jae-Hyun ; Ku, Sae-Kwang ; Lee, Hyeung-Sik ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 207~213
To investigate ultrastructural changes on the adrenal cortex of the developmental rats, tissues were collected at 20 days of gestation, at birth, 7 days, 15 days and 30 days after birth and studied by transmission electron microscopy (TEM) the mitochondrial cristae of zona reticularis in the adrenal cortex of rats were a vesicular type and the vesicles were formed prior to 20 days of gestation. Also, the numbers of vesicles were
in 20 days of gestation,
in 7 days,
in 15 days and
in 30 days after birth, In this study, it was identified that the vesicles of mitochondrial cristae were formed by budding. The dense bodies were also observed in the nuclei of cortex cells from 20 days of gestation to 30 days after birth.
Ultrastructural Studies on the Adrenal Medulla of The Developmental Rats
Park, Joo-Hee ; Lee, Jae-Hyun ; Ku, Sae-Kwang ; Lee, Hyeung-Sik ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 215~224
To investigate ultrastructural changes on the adrenal glands of the developmental rats, tissues were collected at 20 days of gestation, at birth, 7 days, 15 days and 30 days after birth and studied by electron microscope. In the medulla of adrenal gland of the rat, two types of granules were observed at ultrastructural level. The one were adrenaline
and the others were noradrenaline (
of long axis,
of short axis). Adrenaline granules were characterized by presence of round or spherical with low or moderate electron density. The granules showed a wide lucent or clean halo between the contents and the limiting membrane. On the other hand noradrenaline granules were characterized by the presence of irregular shape with high electron density. This granules showed a wide lucent between the contents and limiting membrane. These two types of granules were co-existed in one cell in the late gestation and at birth, but after that two types of cells were clearly observed. The different sizes and numbers of granules were observed with ages.
Effects of DMTU and SOD on Ultrastructural Changes of Gastric Chief Cells in Adriamycin Treated Rats
Paik, Doo-Jin ; Chang, Hyung-Shim ; Chung, Ho-Sam ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 225~236
Adriamycin is a one of anthracyclin antibiotics isolated from the culture media of Streptomyces peucetius var casius. The formation of reactive oxygen metabolite by redox cycling during the metabolism and the inhibition of DNA synthesis results in antineoplastic effects of adriamycin. The authors have demonstrated the effects of SOD(superoxide dismutase) or DMTU (dimethyl thiourea), which are used as an antioxidant, on the ultrastructural changes of the gastric chief cells after the administration of adriamycin in the rat. Adriamycin (30 mg/kg) was administered intraperitoneally to the Sprague-Dawley rats weighing about 220 gm and SOD (15000 unit/kg) or DMTU (500 mg/kg) were administered intraperitoneally to the rats 30 minutes after the administration of adriamycin. The gastric chief cells 24, 48 and 72 hours after the administration of adriamycin were observed with Hitachi-600 electron microscope. The results were as follows. 1. SOD or DMTU alone did not affect the ultra structures of the gastric chief cells in the rat. 2. Dilation, sacculation and segmentation of the cisternae of rough endoplasmic reticulum, dilation of the saccules of Golgi complex and dilated mitochondria with electron lucent matrix were seen in the adriamycin treated rats. In the course of time, the ultrastructures of the chief cell changed markedly. 72 hours after drug administration, severely dilated cisternae of rough endoplasmic reticulum, with clumping of chromatin around the nuclear envelope and mitochondria with electron lucent matrix and dilated cristae were seen in the chief cell. 3. The treatment of SOD is more effective than DMTU to attenuated the ultrastructural changes of the chief cells in the adriamycin administered rat. Consequently it is suggested that adriamycin would induce the degenerative changes of the organelles of the chief cell. The treatment of SOD is more effective than DMTU to attenuate the adriamycin induced damage.
Comparative Ultrastructure of the Fertilized Egg Envelope in Three Species, Cyprinidae, Teleost
Kim, Dong-Heui ; Reu, Dong-Suck ; Deung, Young-Kun ;
Applied Microscopy, volume 28, issue 2, 1998, Pages 237~253
The structures of the fertilizedegg envelope in threespecies of cyprinidae, leoparddanio (Brachydanio frankei), cherry barb (Barbustitteya) and white cloudmountain fish (Tanichthys albonubes) were investigated by light and electron microscopy. The fertilized eggs of all three species have colorless, transparent, spherical and non-floted type. The egg envelopes have a single micropylewhich is thought to the pathway of sperm in the area of the animal pole. Also, an outer layersof both cherry barband white cloud mountain fish areadhesive types, but in that of leoparddanio has non-adhesivetype. In leopard danio, an outer surface ofegg envelope is covered by mushroom-likestructure, that of cherry barb is arranged by button-like structure, and that of white cloud mountain fish isarranged by rod-likestructure. Inboth leopard danioand cherrybarb, the eggenvelopes consist of three distinct layers; an outer layer, a middle layer and an inner layer, and that of white cloud mountain fish hastwo layers. An innerlayer of egg envelopein leopard danio consist of 10 layers, cherry barb has 8 layers, and white cloud mountain fish has