Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
Korean Society of Electron Microscopy
Editor in Chief :
Volume & Issues
Volume 28, Issue 4 - Dec 1998
Volume 28, Issue 3 - Sep 1998
Volume 28, Issue 2 - Jun 1998
Volume 28, Issue 1 - Mar 1998
Selecting the target year
Microglial Reaction to the Cytotoxicity of 5,7-Dihydroxytryptamine in the Cat Retina
Joo, Woo-Hyun ; Nam, Seong-Ahn ; Jo, Seung-Mook ; Cho, Hyon-Hoo ; Shin, Min-Cheol ; Won, Moo-Ho ; Choi, Chang-Do ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 425~434
This study was designed to investigate the microglial reactions to the neurodegenerative changes in the cat retina. All experiments were performed using adult cats of both sex, weighing
dissolved in 0.1% ascorbic acid was injected into the vitreous body. All injections were performed in one-side eye; the other side served as the control, which was injected only with 0.1% ascorbic acid. Cats were sacrificed at 1, 3, 7, 14 and 21 days after intravitreal injection of 5,7-DHT For light microscopy, retinae were fixed with 4% paraformaldehyde and processed using NDPase histochemistry. Same retinae were fixed with 1% para(formaldehyde-2.5% glutaraldehyde and processed for electron microscopy. NDPase-positive microglial cells were mainly distributed in the inner plexiform layer of the retina, and characterized by a small somata with a few slender processes, which were also extended in the ganglion cell layer (GCL) and inner nuclear layer (INL). The intensity of the microglia stained for NDPase was abruptly increased at 7 day as compared with that of the control, and thereafter continuously sustained until 21 day, the last experimental group in this study. Under the electron microscopical observation, microglial cells in the control group exhibited elongate nucleus with perinuclear chromatin condensation, and the perikaryon was scanty. However, a few hypertrophic glial cells were frequently found at 3 days after the drug injection. By 7 day, most microglial cells directed toward the degenerated neurons in the GCL, and the number of microglial cells was slightly increased as compared with the former group. At the 14 day, most microglial cells wrapped the degenerated cells in the GCL, and a few cells showed phagocytotic features. By 21 day, most microglial cells were engaged in phagocytotic activity, and their cytoplasm was filled with the phagorytosed material. Based on the results, 5,7-DHT may act as a specific neurotoxin to the cat retina, and microglial reactions to the neuronal death are already induced in early experimental stage. These results indicate that the microglial cells in the cat retina show characteristic features as a protective effect of neural tissue.
Ultrastructure and Filtrating Function of the Ciliated Epithelial Cells of Foregut in Urechis unicinctus
Shin, Kil-Sang ; Lee, Sun-Hee ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 435~446
It is suggested that Urechis unicinctus is a filler feeder as like many tide and watery invertebrates which filtrate food materials by ciliary movement. However, the structure of the filter is not yet known in U. unicinctus, nor the filtering mechanism is not well understood. This study reveals ciliated epithelial cells in the foregut and the features of the cilia are good accord with that of known filtrating apparatus of other tide animals. This may implies that the foregut is in function of filtration and the food materials are filtrated by the ciliary movement. With the observation of the filtrating apparatus in the foregut, the intestine of U. unicinctus can be functionally compartmented into 3 parts. These are already known midgut and hindgut in function of digestion and respiration respectively, and the foregut in function of filtrating apparatus for foods. The filtrating apparatus of U. unicinctus is composed of the pseudostratified columnar epithelial cells with numerous cilia. The cilia are well differentiated kinocilia with the typical microtubule pattern, kinetosome and cilia roots. There are two kinds of striated cilia roots, the main root and the accessory root. The main root is extended perpendicularly from the cell surface to basement membrane and the short accessory root is branched with an acute angle of about
from the main root at level of basal plate of the kinetosome. The spacial approaches of the main root with the large fused form of mitochondria is one of the characteristic features which might be in structural consideration an intimate association between energy source and energy mass consuming cell organelles.
Ultrastructural Changes of Oocytes from Vitellogenesis into Maturation in Korean Catfish, Silurus asotus
Yoon, Jong-Man ; Kim, Young-Gill ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 447~463
This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 2-year-old female catfishes (Silurus asotus). Female Korean catfishes collected in the vicinity of Chollabukdo have synchronous ovaries which discontinously ovulate eggs during the breeding season (from late May until early July). The fishes were killed by severing the spinal cord just posterior to the head after immobilization with tricaine methanesulfonate (MS 222). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were examined by means of light and transmission electron microscopy. The size of the nucleoli and number of the yolk granules increased as the oocyte groved. Yolk granules were deposited in the oocyte as fluid Due to tile presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and greenish in color. As the percentages of fish in late maturing oocyte (LMO) and ripe oocyte (RO) stage increased from March to April, mean gonadosomatic index (GSI) values (19.95%) increased. Zona radiata changed from squamous into cuboid in shape in the early maturing oocyte (EMO) stage. Processes, microvilli, from the zona radiata and from the oocyte grow, and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radials becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both gonadosomatic index and macroscopic appearance. The main cytological changes such as increase in size of cell, nucleus, nucleolus, and increase in number of nucleoli and mitochondria demonstrated with electron microscopy in the previtellogenic oocytes of Korean catfish, provided evidences for important synthetic processes in an early preparatory phase of oocyte development. The electrophoretic pattern of major band in mature stage was much thicker (24 k, 66 k, 90-110k dalton) than that in previtellogenic phase.
Preservation of Ultrastructure of Ultrathin Frozen Sections for Immunoelectron Microscopic Observation
Kim, Yun-Sang ; Chae, Hee-Sun ; Kim, Kyung-Yong ; Lee, Won-Bok ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 465~475
The cryoprotection, section retrieval and embedding methods were studied for the preservation of ultrastructure of ultracryomicrosections in immunoelectron microscopy. The results obtained were as follows. 1. The cryoprotection of ultrastructure with a mixture containing 1.7 M sucrose and 15% polyvinylpyrrolidone was better than that with 2.3 M sucrose. The stretching caused by surface tension and the electron lucent holes decreased more in the cryosections infused with 2.3 M sucrose than in those with the mixture. 2. The difference between section retrieval solutions in cases of cryoprotection with 2.3 M sucrose was that the destructive .effects such as electron lucent holes and stretching between myofribrils were less in a mixture containing 1% methylcellulose and 2.3 M sucrose than in 2.3 M sucrose. The difference was obscure in the mixture containing 1.7 M sucrose and 15% PVP, but the destructive effects were slightly less in a mixture containing 1% mthylcellulose and 2.3 M sucrose than in 2.3 M sucrose or 1% methylcellulose. 3. The embedding of cryosection on drying with 2% PVA or 2% methylcellulose exhibited some protective effect during observation with transmission electron microscope, but made the ultrastructure more obscure. 4. Mitochondrial membrane and cristae and myofilaments were well delinated in sections infused with 2.3 M sucrose and retrieved with 1% methylcellulose and 2.3 M sucrose. In summary, it is suggested that the cryoprotection with 2.3 M sucrose and section retrieval with a mixture containing 1% methylcellulose and 2.3 M sucrose are good for the ultrastructure of cryosections.
Ultrastructure of the Eye in the Atypus coreanus Kim, 1985
Kwon, Jung-Kyun ; Ko, Myung-Kyoo ; Chung, Ho-Sam ; Kim, Joo-Pil ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 477~490
Most spiders have four pairs of simple eyes. A few families of the spider have their developed eyes to an extent visual cues make up a significant of information to response from the external stimuli. Most spiders respond to the external stimuli around them. Specially, they are very sensitive to vibrations from the air, the ground, their webs, or even the surface of water. The present study was undertaken to examine the evolutionary development and function of eye with the observation of visual ultrastructure of Atypus coreanus Kim, 1985 using the electron microscopy. They have weak mobility, limited territory and low developed eyes. Atypus coreanus was collected from Mt. Ungil, Namyangju-gun, Kyonggi province. The fine structure of these eyes was examined by electron microscopy, and prepard by teasing method for scanning electron microscopic observation. As a result, the eyes of Atypus coreanus was composed of cornea, lens, vitreous body, retina, and rhabdome.
An Ultrastructural Changes of Rat Corneal Epithelium and Stromal layer in Developmental Process
Kim, In-Suk ; Kim, Jin-Suk ; Jeon, Jin-Seok ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 491~502
The present study was conducted to investigate the structural changes in rat cornea. Sixty eyes from one-day-old uneyed rats, fourty eyes from 4-weeks-old rats, and foully eyes from 10-weeks-old adult rats were used. With the increase of age, the epithelial layer was thickened by the addition of new successive cellular layers. Then, the new-born rat's epithelial cells formed a pentagonal shape, and the quality of decidual cells showed a high electron-density, although the boundary between cells was distinctive. The newly produced cells showed a low electron-density so that there was the distinctive difference between light and darkness. In Bowman's layer, collagen fibrils demonstrate a regularly arranged structure along with the age. In stroma's layer, the density of keratocytes was decreased and thereby Progressively flattened during the development. The collagenous layer of the adult rats was more distinctive than that of the new-born rats in a form of parallel alignment running vertically and horizontally.
Fine Structure of the Cutaneous Pigments in the Black Widow Spider, Latrodectus mactans
Moon, Myung-Jin ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 503~512
Fine structure of the cutaneous pigments in the black widow spider, Latrodectus mactans are studied with light and electron microscopes. The cutaneous pigments are only observed in epidermal layer just beneath the cuticle. These pigments are compactly distributed around the spinnerets which located at caudal area of the abdomen. According to the fine structural characteristics of the pigment granules, two main types of guanine pigment granules-carotenoid vesicles and reflecting platelets - are observed in the cytoplasm of the epidermal cells. Morphological features of these pigment granules are characterized as the electron dense carotenoid vesicles and the electron lucent reflecting platelets. Marginal electron density of the carotenoid vesicle is different from that of internal region, whereas the reflecting platelets have laminated crystalline granules. Typiral structures of these pigment granules are very similar to those of invertebrate's chromatophores, especially erythrophores and iridophores. Moreover differentiation of these pigment granules are also originated from the small vesicles of Golgi complexes similarly to those of cutaneous chromatophores.
A Ultrastructural Study on the Axoneme Formation in the Spermatozoa of the Edible Giant Snail, Achatina fulica
Chang, Nam-Sub ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 513~525
The spermatogenetic process in the edible giant snail is similar to those in the other snails, except for the axoneme formation process. In this study, the axoneme formation process in the giant snail was mainly examined by means of electron microscopy. The tail portion of a spermatozoon is about
long, and extends straight to the rear, surrounded by two large and long mitochondria in spiral forms. A number of glycogen particles
are found in the swollen matrix of the mitochodria. The axoneme which composes the tail of a spermatozoon is surrounded by
lamella-form fibrous sheaths of about
in thickness. Most of the mature spermatozoa are found to be clustered into a group of
ea in syncytial bridges formed by cytoplasmic processes. Sertoli cells contain glycogen particles, endoplasmic reticulum, a lot of mitochondria, and lipids in their cytoplasm. They protrude their filiform pseudopodia and phagocytize abnormal spermatids or spermaozoa.
A Study on the Activity of Antibodies Immunreaction to the Antigen of Paragonimus westermani
Kim, Soo-Jin ; Lee, Ji-Hyon ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 527~538
This study was performed to investigate the activity of antibodies in the tissues of Paragonimus westermani at the different developmental stages. Enzyme-linked immunosorbent assay (ELISA) and Immunelectron microscopy (IEM) were applied, using the dog sera infected with metacercariae isolated from Cambaroides similis. These dog sera were obtained from 3rd to 96th week after infection by bleeding. The supernatants of homogenated worms for worm antigen were used. The worm tissues were embedded in Lowicryl HM 20 medium, treated with infected serum and protein A gold complex (particle size; 12 nm) and observed by electron microscope. In the pattern of antibody levels by ELISA test in all developemental worm antigens, the activity of antibody was very weak in the 3rd week, but strong in the worm antibody from 4th to 20th week after infection. Its activity was maintained even till 96th week. The antibody level of the L2th week worm antigen was higher than those of the 20th and 48th week worm antigens. Generally, many gold particles were observed on the secretory granules and the epithelial lamellae. Thus, it was concluded that the antigenic materials in the developmental worm tissues were especially concentrated on the secretory granules in the parenchymal tissues and the epithelial lamellae in the lumen of the caecum.
Changes of Tubuliform Silk Glands during the Cocoon Production in the Garden Spider, Argiope aurentia
Moon, Myung-Jin ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 539~549
The silk glands of the spiders are of several types. Among the several types of spider's silk glands, the principal fibers used in constructing the eggcase are products of the tubuliform glands (TBG), which are present only in females. Development of these glands parallels maturations of the ovaries and fat bodies. In order to understand the mechanism of eggcase-silk production, this paper has examined the fine structural changes of the TBG during the period of egg maturation in the garden spider, Argiope aurentia. Between the two kinds of secretory granules observed in the glandular epithelium of the mature TBG, the electron-dense granules which have paracrystalline structure are revealed to be the precursors of the eggcase silk fibers. During the production of eggcase, rapid release of the secretory product occurs at apical surface by the mechanism of apocrine secretion. Moreover, secondary lysosomes appear due to the rapid disorganization of cellular components during the eggcase formation. Examinations of formed fibers indicate a multicomponent internal structure, and electron micrographs reveal each fiber contains numerous electron lucent fibrils embedded in an amorphous electron dense matrix. The secretory precursors are produced as separated vesicles via well-oriented rER, and no Golgi complex has been found in the glandular epithelial cells.
Ultrastructural Localization of Mercury in Spleen of the Mouse
Cho, Hyun-Wook ; Kim, Myung-Hoon ; Yee, Sung-Tae ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 551~561
The autometallographic method was used to demonstrate the localization of mercury deposits in spleen of mouse. The mercury deposits were identified with the light and electron mocroscope. Mice were treated with methylmercuric chloride in the drinking water (demineralized water) for 40 days. Control and mercury treated groups showed no significant differences in mean body weight and spleen weight per one mouse. Mercury grains were appeared in the germinal center of white pulp consist of a preponderancing lymphocytes, not in red pulp and capsule. At the ultrastructural level, mercury deposits were restricted to lysosomes of macrophage and lymphocyte. Specially, volume in lysosomes of the macrophage was increased. These results suggest that mercury localization in lysosomes is associated with the change of immune activity.
Ultrastructure and Dehydrogenase activity on the Differentiation of the Cerebral Nerve Cell in the Chick Embryo (1)
Kim, Saeng-Gon ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 563~575
To investigate the changes during the differentiation of the cerebral neurons of chick embryo of tne embryogenic day (ED) 7 and 8, the ultrastructural changes in the cerebral neurons, the activity of dehydronases (LDH, MDH and SDH), protein expression profile and adenosine triphosphate concentration were analyzed. In ED 7 chick embryos, relatively large nucleus, centrally located nucleolus, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. Oval-shaped mitochondria with well-developed cristae were present over entire cytoplasm. In ED 8 chick embryos, evenly spread chromatin over nucleoplasm, and prominent nuclear envelope were observed. In the cytoplasm, well-developed rough endoplasmic reticulum and Golgi complex were observed. In ED 7 chick embryos and ED 8 chick embryos, 31 polypeptide bands and 34 polypeptide bands were observed, respectively. The activities of dehydrogenases were lower in ED 7 chick embryos than in ED 8 chick embryos. LDH activity was 8.16 (ED 7) and 9.28 (ED 8), MDH activity was 7.98 (ED 7) and 10.10 (ED 8), and SDH activity was 5.49 (ED 7) and 7.14 (ED 8) respectively. The ATP concentration remained unchanged over ED 7 and 8.
Morphological Changes in Glomerular Podocytes in Puromycin Aminonucleoside Induced Nephropathy
Kim, Young-Ho ; Park, Kwan-Kyu ; Kim, Young-Man ; Cho, Soo-Yeul ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 577~590
Puromycin aminonucleoside (PAN) nephropathy was induced in a group of Sprague-Dawley rat by a single dose of intraperitoneal Injection to study an ultrastructural change of glomerulus. The experimental rats developed proteinuria three days after PAN injection. Electron microscopic studies of glomeruli showed the loss of epithelial foot processes, formation of cytoplasmic vacuoles, microvillous formation and increased numbers of lysosomes in the cytoplasm of podocytes. It is strongly suggested that proteinuria in PAN nephrosis may be primarily due to a glomerular epithelial lesion, leading to focal disarray of anionic sites or focal defects in the epithelial covering of the basement membrane. The loss of anionic sites in the basement membrane nay be caused by the foot process fusion and the epithelial detachment from the basement membrane.
Irradiation in Central Nervous System and Recovery Effect: Action of Antioxidants and NMDA-receptor Antagonists
Mun, Yeun-Ja ; Park, Seung-Taeck ; Choi, Min-Kyu ; Jeong, Dong-Hyeok ; Moon, Sun-Rock ; Chung, Yeun-Tai ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 591~601
The neurotoxic effects of radiation have been studied in NSC-34 hybrid cells derived from embryonic mouse spinal cord cells. NSC-34 cells irradiated at 25Gy were decreased the cell viability in a time and dose dependent manner. The decrease in cell viability induced by the irradiation was blocked by catalase. Antagonists of the N-methyl-D-aspartate (NMDA) receptor, including D-2-amino-5-phosphonovaleric acid (APV) and chlorokynurenic acid (CKA), similarly blocked radiational induced in cell viability. We performed morphological analysis of light and electron microscope. NSC-34 cells irradiated at 25Gy were decreased the cell density and increased lysosomes and vacuoles in the cytoplasm. Especially chromatin modification was observed. These results indicated that radiation was involved in the oxidant-initiated neurotoxicity and the compounds catalase, APV and CKA were shown to be neuroprotective against radiation.
Electron Microscopic Observations on the Endoplasmic Reticulum and Golgi Complex during Spermiogenesis in the Long-Fingered Bat (Miniopterus schreibersi fuliginosus Hodgson)
Choi, Byung-Jin ; Son, Sung-Won ; Lee, Jung-Hun ; Lee, Kae-Il ;
Applied Microscopy, volume 28, issue 4, 1998, Pages 603~613
The present study was designed in order to observe relationship between the endoplasmic reticulum and the Golgi complex during spermiogenesis of the long-fingered bat (Miniopterus schreibersi fuliginosus). The testes were obtained from adult bats and treated with the prolonged osmification or fixed with ferrocyanide reduced osmiun. In the Golgi phase, The Golgi complex shows an oval shape, and was composed of a cortex and a medullar enclosing acrosome. The Golgi vacuoles with electron-dense granules of crescent shape were fused with each other. The smooth endoplasrnic reticulum was scattered in all the area of the cytoplasm. In the cap phase, The Golgi complex was crescent in shape, and faced to a nucleus. Large and small vesicles were fused with each other, and then fused with a acrosomal vacuole. The rough endoplasmic reticulum was close to the large Golgi vacuole. In the acrosome phase, The Golgi complex was moved to behind of the acrosome face. Small vesicles were fused with an acrosome, and cisternae of the trans-face of Golgi complex was connected with an acrosome in the early acrosome phase. The smooth endoplasmic reticulum was distributed in the cytoplasm. The annulate lamellar was originated from a radial body-annulate lammellae complex. In the maturation phase, The Golgi complex with dilated cistrern appeared in the cytoplasm, and also, annulate lamellar was observed in the cytoplasm. The connection of the annulate lamellar with the cistern of radial body suggests that an annulate lamellar seems to be closely related to radial body. The smooth endoplasmic reticulum was scattered in the cytoplasm in the early Golgi phase, but annulate lamellar-radial body complex which might be a residual and disappearing form of the smooth endoplasmic reticulum appeared in the acrosome phase. The Golgi complex steadily remained in the late maturation phase when the endoplasmic reticulum began to disappear from the cytoplasm: the Golgi complex was still occurred after acrosome formation. The observations obtained in the present study, which was characterized by the presence of the Golgi complex in the late maturation phase, suggests that the Golgi complex may play an important role also even after the acrosome formation.