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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume 29, Issue 4 - Dec 1999
Volume 29, Issue 3 - Sep 1999
Volume 29, Issue 2 - Jun 1999
Volume 29, Issue 1 - Mar 1999
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Morphological Comparison of Spermatozoa in the Korean Greater Horseshoe Bat (Rhinolophus ferrumequinum korai) and Long-Fingered Bat (Miniopterus schreibersi fuliginosus)
Kim, Sang-Sick ; Lee, Jung-Hun ; Son, Sung-Won ; Choi, Byung-Jin ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 1~10
The aim of the present study was to investigate with transmission electron misroscope the comparative morphology of epididymal spermatozoa in two species of the Korean insectivorous bats belonging to 'prolonged sperm storage' type (Rhinolophus ferrumequinum korai) and 'delayed implantation' type (Miniopterus schreibersi fuliginosus). Sperm head of the R. ferrumequinum korai was bullet shaped and that of M. schreibersi fuliginosus was spatula shaped. The nuclei of the sperm head of the R. ferrumequinum korai and M. schreibersi fuliginosus occupied two-third and a half of it, respectively. The segmented columns of R. ferrumequinum korai were about 12 to 14 in number, and those of the M. schreibersi fuligincsus were about 10 to 12. Particularly, a pile of the satellite fibers in middle piece of R. ferrumequinum korai remained the inner aspect of the outer dense fibers, but those of the M. schreibersi fuliginosus were not.
Ultrastructural Study on the Ependymal Cells of the Read-Irradiated Rats
Ahn, E-Tay ; Cho, Hwee-Dong ; Kim, Jin-Gook ; Park, Kyung-Ho ; Ko, Jeong-Sik ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 11~23
Ultrastructure of the ependymal cells of X-irradiated rats on their head were studied. Rats weighing
were X-irradiated on their head and neck areas. Total exposures were 3,000 rads or 6,000 rads depending on experimental groups. And irradiated rats were sacrificed on 6 hours, 2 days and 6 days following the radiation exposures. Animals were perfused through the heart with 1% glutaraldehyde-1% paraformaldehyde solution, under ether-anesthesia. The tissues from the wall of lateral ventricles were fixed in the 2% osmium tetroxide solution. The results observed with electron microscope were as follow: 1. In 6 hours group, many ependymal cells were swelled, luminal portions of cytoplasms of some cells protruded into the ventricular lumen, and many cilia were lost or irregularly altered. 2. In 2 days group, ependymal cells were swelled more severely and subependymal edema were pronounced. 3. Protruded cytoplasm contained usually basal bodies of cilia, groups of mitochondria, endoplasmic reticula , etc. 4. Following X-irradiations, some protruded masses contained neural elements including the axon terminals with dense core vesicles. Axons and axon terminals were also found in the enlarged intercellular spaces among ependymal cells. From the above results, the heavy irradiation on the head area of the rat induced alteration of the ependymal cells lining the lateral ventricle. Hence the ependymal functions of selective barrier, protective barrier, and metabolic barrier could be altered following X-irradiation on the head.
Fine Structural Changes of the Renal Corpuscle of the Mice following the Administration of 5-Fluorouracil or Mitomycin C
Ko, Jeong-Sik ; Oh, Won-Young ; Kim, Jin-Gook ; Park, Kyung-Ho ; Ahn, E-Tay ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 25~41
The experiment was performed to study the morphological responses of the renal glomeruli of the mice after administration of 5-fluorouracil or mitomycin C. 5-fluorouracil (60 mg/kg) or mitomycin-C
were injected subcutaneously to the animals every other day, and animals were sacrificed at 4 days or 7 days following the first injections. Pieces of tissues were observed with a JEM 100CX-II electron microscope. The observed results were as follows: 1. In the fourth day following the first injection of 5-fluorouracil or mitomycin C, components of the renal glomeruli of the mice are looked compact since they were filled with the widened the mesangium, and showed narrowing lumen of glomerular capillaries and of urinary spaces. The changes were more significant in the mitomycin C treated mice. 2. In the 5-fluorouracil treated mice, morphological changes of glomeruli were generally recovered in the seventh day, whereas the glomeruli of the mitomycin C treated mice have not shown general recovery. 3. In the fourth and seventh days following the first injection of mitomycin C, in the renal glomeruli of the mice, swollen endothelial cells, and protruded mesangeal cells into the capillary lumen are frequently observed. 4. In the fourth day following the first injection of mitomycin C, in the glomerular basal lamina of the mice, the electron densities of the lamina rara interna and the lamina rara externa were similar to the density of the lamina densa and the expanded lamina rara interna were often seen. From the above results, it is suggested that the cytotoxic effects of the mitomycin C on renal glomeruli are more severe as compared with those of 5-fluorouracil.
Rotations between Erythroblasts and Kupffer Cells in Human Fetal Hepatic Erythropoiesis - Trasmission and Scanning Electron Microscopic Observation
Lee, Won-Bok ; Shin, Do-Shik ; Kim, Kyung-Yong ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 43~56
The relationship between intravascular erythroblasts and Kupffer cells in the human fetal liver from 11 to 20 week gestation was studied ultrastructurally. The walls of the developing sinusoids consisted of two cell types devoid of basal lamina, the nonfenestrasted endothelial cells and Kupffer cells. Kupffer cells examined were easily identified by their content of phagosmes and their morphological features, and partially proliferated by mitotic division which was different way of proliferation from adult. Some extruded nuclei of acidophilic erythroblasts were trapped within Kupffer cells which exhibited various stages of intracellular digestion of the nuclei. During high activity of human fetal hepatic erythropoiesis, Kupffer cells were found in association with developing erythrob-lasts, which was similar with erythroblastic islands. The developing erythroblasts were partially surrounded by multilaminated membrane system of the Kupffer cell consisting erythroblastic island, or in contact with Kupffer cell via cytoptasmic processes in the sinu-soidal lumen. The presence of these islands was confirmed by transmission and scanning electron microscopic study. The results demonstrate that Kupffer cells in fetal heaptic erythropoiesis phagocytized expelled nuclei and contributed to erythropoiesis mechanically and physiologically by the hypertrophy and the formation of erythroblastic islands.
Eine Structure of Cerebral Ganglion in the Korean Planaria, Dugesia japonica
Chang, Nam-Sub ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 57~66
The nervous tissue in the cerebral ganglion of Korean planaria was observed using electron microscope. The obtained results are as follows: A cerebral ganglion is composed of the nerve cells, neurosecretory cells, neuroglial cells and neuropils. The nerve cells are round or ovoidal-shaped cells (diameter,
), which has a large ellipsoidal nucleus containing the evenly developed heterochromatin. Their cytoplasms were found to be relatively simple, because of their undeveloped cell organelles. The neurosecretory cells are long and ellipsoid or spindle-shaped cells, where there were found a large ellipsoidal nucleus and cytoplasm filled with secretory granules (diameter, 60 nm). The neuroglial cells were seldom observed. They are spindle-shaped cells (size,
), which were observed mainly among the nerve fibers. The neuropils are formed by the nerve fibers and nerve endings which are filled with mitochondria, neurotubules and secretory granules of four kinds (high electron dense granules of sizes 75 nm, 50 nm and 37 nm, and electron lucent granule of size 30 nm etc.). These granular vesicles are divided into single vesicle type and compound vesicle type in the nerve terminals, and neuronal synapses were observed to be the axo-dendritic and dendro-dendritic synapse type.
An Investigation of TEM Specimen Preparation Methods from Powders Using a Centrifuge
Jeung, Jong-Man ; Lee, Young-Boo ; Kim, Youn-Joong ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 67~73
It is practically hard to prepare good TEM specimens from powders which are embedded in epoxy materials for ion milling, because the milling rate difference between powders and epoxy is quite large. In order to overcome this problem, we tried to find methods to increase the density of powders in the embedding epoxy without loosing the adhesive strength between them. Powder density was considerably increased by employing a centrifuge for embedding, compared to the result by a conventional vacuum embedding. In addition, mixing powders of different sizes after sieving also enhanced the final density by allowing smaller particles filling in the gaps of larger particles. Ion milling of powders embedded by these methods resulted in thin specimens good enough for normal TEM works. TEM specimens from spherical, platy and fibrous powders of submicron size were successfully prepared by this centrifuging method.
An Investigation of Lattice Parameter Measurement of Inorganic Crystals by Electron Diffraction Patterns
Lee, Young-Boo ; Kim, Youn-Joong ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 75~81
Optimum conditions for making the Au and Al internal standards for TEM have been determined experimentally. The Au internal standard was produced by sputter coating at 9mA for 100 seconds in low vacuum
. The Al internal standard was produced by evaporation coating at 7kV for 10 minutes in high vacuum
. Measurements of the lattice parameters of andalusite and albite feldspars with this Au internal standard resulted in errors of (a)
in precion and
in accuracy for andalusite: (b)
in precision and
in accuracy for albite feldspars. The most significant error occurred from the measuring processes of distances and angles of electron diffraction patterns. By employing systematic procedures of measurement with high precision devices, this lattice parameter determination method utilizing the internal standard should be a good alternative to the conventional powder XRD method or the sophisticated CBED method for special samples.
Eine Structure of Digital Arteries in Rat
Kim, Baik-Yoon ; Shin, Keun-Nam ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 83~94
The ultrastructure of small arterioles and capillaries in the lumbrical muscles of rat digits were studied by electron microscopy and following results were obtained. 1. The diameter of small arterioles of rat digits were
, and it was relatively smaller than human
. 2. All the endothelial cells of small arterioles and capillaries in the lumbrical muscles of rat digits were continuous type, and they had typical morphological characteristics of continuous endothelial cells : numerous cytoplasmic pinocytic vesicles and many tight junctions between the endothelial cells. 3. In the small arterioles subendothelial layers were irregularly spaced beneath the basal lamina, and membrane to membrane contacts were found between the endothelial cells and smooth muscle cells. 4. Pericytes were often found nearby capillary endothelium, and their cytoplasmic processes surrounded part of endothelial cells. They were enclosed by basal lamina. 5. Smooth muscle cells in tunica media of small arterioles were only one layered, that is, they were terminal arterioles. Sarcoplasm of smooth muscle cell was divided to 2 areas; homogeneous or filamentous area and non-homogeneous perinuclear area. 6. The tunica adventitia contained fibroblasts with extremely attenuated cytoplasmic processes and collagen fibirls.
HRTEM Observations on the Modulated Structure in Pseudo-brookite-type Compound,
Lee, Hwack-Joo ; Park, Hyun-Min ; Cho, Yang-Koo ; Ryu, Hyun ; Nahm, Sahn ; Bando, Y. ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 95~103
Microstructural observations on the pseudo-brookite
and the similar type of
were carried out using a top-entry HRTEM working at 200 kV. The modulated structures were found in
, however, not in
. The electron diffraction patterns of sublattice in
are quite similar to those of pseudo-brookite
. but the complicated superlattice reflections are present in the diffraction patterns. Four types of modulations have been found. The periodicities for the modulated structure are found to be 3.63 nm, 0.79 nm and 0.64 nm along  direction, and 0.81 nm along  direction. The phase transition from the modulated structure to the unmodulated one was also observed in situ due to the electron beam irradiation reversibly. Further damage by the electron beam made the crystal to be fragmented into many small crystals with the formation of the voids at the kinks in ledged structure of the surface. The anisotropic arrangements of In and O atoms in
might cause the compound to be unstable under the electron beam.
Histological and Ultrastructural Differentiations of the Neuroepithelial Cells in the Mouse Embryo during Early Neurulation
Kim, Yul-A ; Chung, Young-Wha ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 105~124
Histological and ultrastructural differentiations of the neuroepithelial cells in the mouse embryo during neurulation were observed. The neural plates and grooves consisted of pseudostratified columnar epithelium in the embryonic day (ED) 8 embryo were developed. In the ED 9 embryo, the neural tube was developed in all body length of embryo except both the cephalic and caudal ends. Secondary neurulation was shown at the tail bud of the ED 10 embryo. In the ED 8 embryo, the primitive streak was shown in the posterior end of the embryonic disc. The neuroepithelium, notochord and mesenchyme were well differentiated in the cephalic and cervical portions. In the ED 9 and 10 embryos, the roof plates of neural tubes were constituted of the closing of the surface ectodermal cells in the hindbrain and the neuroepithelial cells in the spinal cord. The floor plate of neural tube were consisted of the low pseudostratified columnar epithelium. The spinal motor nerve fibers were initially differentiated in the ED 10 embryo. According to the electron density of the cell and the differentiation of tell organelles, the neuroepithelial cells in the ED 9 and 10 embryos were classified into three types: dark, intermediate and light types. All types in the ED 9 embryo were observed but the dark cell in the ED 10 embryo was not done. The free ribosomes and polysomes in all neuroepithelial cells were developed. The RER and lipid droplets in the dark cell and the Golgi complex in the intermediate and light cells were observed. Many microfilaments in the cytoplasmic processes of intermediate cell and the microfilaments and microtubules in the light cell processes were observed to be well differentiated.
Ultrastructural study of Glandular Trichomes in Pelargonium peltatum
Cho, Bum-Suk ; Ko, Kyoung-Nam ; Kim, Eun-Soo ;
Applied Microscopy, volume 29, issue 1, 1999, Pages 125~136
The ultrastructure of glandular trichomes in Pelargonium peltatum has been studied with a light microscope, transmission, and scanning electron microscope. Two types of the glands, long-stalked and short-stalked capitate glands, are distinguished with their shape and size of the total glands. Both glands are extreamly abundant in the leaf veins and petioles. These glandular trichomes are consisted of one secretory cell, three stalk cells, and one basal cell. The secretory cells contain a large amount of smooth endoplasmic reticulum. They have also much plastids, vacuoles, Golgi apparati, and mitochondria. High electron-dense deposits are frequently present in vacuoles of secretory cells. It seems to be phenolic compounds which is thought as the major secretory precursors.