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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 29, Issue 4 - Dec 1999
Volume 29, Issue 3 - Sep 1999
Volume 29, Issue 2 - Jun 1999
Volume 29, Issue 1 - Mar 1999
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Microstructure Characterization of Cu Thin Films : Effects of Sputter Deposition Conditions
Joh, Cheol-Ho ; Jung, Jin-Goo ; Kim, Young-Ho ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 265~274
The microstructure of Cu thin films in various deposition conditions was characterized. Cr films (50 nm thick) and Cu films (500 or 1000 nm thick) were deposited on polyimide films by DC magnetron sputtering. The Ar pressure during Cu deposition was controlled to 5, 50 and 100 mtorr. The microstructure was characterized using conventional and high resolution SEM and TEM. As sputtering pressure increases, open boundaries are observed more frequently. The Cu film deposited at 5 mtorr has a dense and uniform structure, while low-density regions or open boundaries between columns exist in the film deposited at higher pressure. As the film grows thicker, open boundaries are wider and the density of open boundaries are higher. The comparison between SEM and TEM show that the small features shown in high resolution SEM are grains. High resolution SEM is very effective to characterize the microstructure of the thin films. One column in the films deposited at 50 and 100 mtorr consists of several grains, which are smaller than those deposited at 5 mtorr.
First Description of Wood Decay Fungi, Hypoxylon moriforme, in Korea
Lee, Yang-Soo ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 275~280
The genus of Hypoxylon, well known wood degrading fungi, is a member of the Xylariales, which has woody to carbonaceous, brown to dark brown stromata. Hypoxylon moriforme, which was isolated from heavily decayed hardwood, is fairly described by electron microscope. The isolation of H. moriforme will be the first record and the first description in Korea. The species collected, having small size of ascospores
, are taxonomically compared with H. truncatum, which is well known as a popular species in Northeast Asia. And H. bovei and H. annulatum, which are the tropic to subtropic species and also compared with other temperate species of H. moriforme.
Thixoforming Characteristics of Metal Matrix Composites (Phase identification of
Lee, Jung-Il ; Kim, Young-Jig ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 281~289
The stirred and thixoformed
Mg composites are studied on the basis of microstructural analysis using transmission electron microscopy (TEM). The products of interfacial reaction are identified as
, MgO and
phases and the crystallized phases are found to be orthorhmbic
and decagonal T phases. It is shown that
phases are found at the surface of
is found near interface and crystallized on the matrix. Phase identification is carried out by crystallographic work based on primitive cell volume, zero order Laue zone (ZOLZ) patterns and single convergent beam electron diffraction (CBED) patterns containing higher order Laue zone ring from a nanosized region.
The Differentiation and Ultrastructural Characteristics of Type II Pneumocyte in Early Human Fetal Lungs
Yoo, Dong-Hwa ; Kim, Dae-Joong ; Kim, Sung-Su ; Kim, Kyung-Yong ; Lee, Won-Bok ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 291~301
The differentiation and ultrastructural characteristics of type II pneumocyte was investigated using 7 cases of human fetal lungs from 9 to 20 weeks of gestation by transmission electron micropscopy. The result obtained were as follows. 1. From the pattern of epithelium, type II pneumocyte was not discernable, but with the gradual development of gestation, the epithelium of the future pulmonary alveoli was transformed from pseudostratified columnar into simple cuboidal epithelium after 15 weeks of geatation. 2. The multilamellar body very specific to type II pneumocyte was observed at first at 9 weeks of gestation. Besides, another characteristics of the cell were also observed such as cytoplasmic inclusion body, granular inclusionbody,multivesicularinclusionbodyanddensebody. 3. The number and size of multilamellar body increased, but those of other inclision bodies decreased at 19 and 20 weeks of gestation. In summary, type II pneumocytes (or precursor cells) with multilamellar body and another characteristic inclusion bodies were observed in the human fetal lungs from 9 weeks of gestation. And so, it is suggested that the differentiation of type II pneumocyte starts at or before 9 weeks of gestation.
A Ultrastructural Study on the Cerebral Ganglion of the African Giant Snail, Achatina fulica
Chang, Nam-Sub ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 303~313
In this paper, five kinds of neurosecretory cells-light green (LG) cell, dark green (DG) cell, caudo-dorsal (CD) cell, blue green (BG) cell, and yellow (Y) cell- and neuropils in the cerebral ganglion of the African giant snail, Achatina fulica, were observed with an electron microscope. The following results were obtained. The LG cells are circular or ovoid in shape, and about
in size. The nucleus and cytoplasm of the LG cell look light due to their electron-low density. Large granular chromatins are evenly developed in the karyolymph, where round nucleoli are also found. In the cytoplasm, electron -high dense round granules of
in average size are crowded. The DG cells are ovoid in shape, and
in size. These relatively electron-high dense cells were rarely found. In their cytoplasm, cell organelles such as rough endoplasmic reticulum and mitochondria are found together with electron -high dense round granules of
in average size. The CD cells are ellipsoidal cells densely distributed in caudo-dorsal parts of the cerebral ganglion. They have large nuclei compared with the cytoplasm. The developed granular heterochromatins are observed in the karyolymph, and lots of small round granules of
in average size in the cytoplasm. The 3G cells, rarely found around endoneurium of the cerebral ganglion, take the shapes of long ellipses. They look dark due to their electron -high density. In the cytoplasm, small round granules of
in average size are found. The Y cells are the smallest among the neurosecretory cells(
in size). They are found mostly between the medio-dorsal parts and the caudo-dorsal parts of the cerebral ganglion. In the cytoplasm, tiny round granules of
in average size form a group. The neuropils are found in the middle of the cerebral ganglion. In the axon ending, round granules with electron -high density (
in diameter) and lucent vesicles (
in diameter) are found in large quantities. They are excreted in the state of exocytosome formed by the invagination of the limiting membrane of the axon ending.
, 25 Dihydroxycholecalciferol on Osteoporotic Fracture : Light Microscopic and Scanning Electron Microscopic Observation
Bae, Chun-Sik ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 315~321
Vitamin D is one of important factors involved in the regulation of bone metabolism. In osteoporosis, the therapeutic effect of vitamin D on the healing process of fracture has still been controversial. These studies were designed to understand the healing process of normal fibular fracture and the therapeutic effects of
, 25 dihydroxycholecalciferol on the osteoporotic fracture in rats. The simple transverse fractures of rat fibulae were produced with a rotating diamond saw. The histological and ultrastructural changes of rats were observed. The histological and ultrastructural studies revealed the healing of the fibular fracture in the 5th week after simple transverse fracture. The osteoporosis impaired more the healing of osteoporotic fibular fracture than normal non-osteoporotic fibular fracture. The healing process of osteoporotic fracture was facilitated by the treatment with
, 25 dihydroxycholecalciferol, however, was delayed more than the healing process of normal fracture. These results suggest that
, 25 dihydroxycholecalciferol was effective for reducing the deleterious effects of osteoporosis in fracture healing.
Electron Microscopy of the Al and
Nanophase Particles Synthesized in Horse Spleen Ferritin
Mun, Hyang-Ran ; Kim, Kyung-Suk ; Lee, Jung-Hoo ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 323~329
Synthesis of inorganic nanophase particles was performed to verify and understand the binding of non-ferrous metal ions including Al and
to the apoferritin molecules. Reconstituted inorganic particles of Al or
were identified by TEM as discrete electron dense cores encapsulated within the protein shell. The corresponding EDXA spectra confirm the presence of metal ions in the reconstituted ferritin. The Al cores of ferritin has been studied by TEM for the first time. Bimetallic cores with Al/Fe and
were also produced and examined under TEM. Mixed metal cores encapsulated in the protein shell are well formed and its corresponding EDXA spectra also confirm the presence of metal ions in the mineral cores. Therefore, the present study proves that ferritin can be used to synthesize inorganic nanophase particles of Al and
A Study on the Morphology of Dysmorphic Erythrocytes for the Differential Diagnosis in Hematuria
Yoon, Chul-Jong ; Park, Chung-Oh ; Moon, Hi-Joo ; Yoon, Ki-Eun ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 331~341
Examination of the morphology of red blood cells in the urine has been shown to be a promising adjunct in determining whether hematuria represents glomerular or nonglomerular bleeding. This is due to distortion of RBCs as they Pass across the basement membrane of the glomerular capillaries. It is concluded that is method can greatly help the clinician in distinguishing between glomerular and nonglomerular bleeding in patients with hematuria and channeling such patients toward the most appropriate investigations. We have experimented dysmorphic red blood cells that 5 patients of the hematuria are distorted with irregular outlines and often have small blobs extruding from the red cell membrane. Tried urinary sediments were seen with phase contrast microscope and confirmed scanning electron microscope. There are seen acanthocytes, anulocytes, ghost cells and sphero-echinocytes in dysmorphic erythrocytes. Clinical diagnosis was referred from the result of the biopsy-proven. Scanning electron microscopic findings of the hematuria are good diagnostic tool that disclose in distorted red blood cells from patients with glomerular disorders.
Comparative Ultranstructures of the Fertilized Egg Envelopes from Three-spot gourami, Pearl gourami and Marble gourami, Belontiidae, Teleost
Kim, Dong-Heui ; Deung, Young-Kun ; Kim, Wan-Jong ; Reu, Dong-Suck ; Kang, Song Jian ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 343~351
The structures of the fertilized egg envelope from three species, three-spot gourami (Trichogaster trichopterus), pearl gourami (Trichogaster leeri) and marble gourami (Trichogaster trichopterus trichopterus) belong to Belontiidae were observed, utilizing light, scanning and transmission electron microscopes. In all three species, the fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. Specially, the micropyle of three-spot gourami was similar to that of marble gourami which is subspecies of three-spot gourami. An outer surface of the fertilized egg envelope was arranged by grooves in all three species. The fertilized egg envelopes consists of two distinct layers; an adhesive outer layer and an inner layer with high electron density. In conclusion, the morphological similarity of the fertilized egg, micropyle, outer surface and transverse section of the fertilized e9g envelope seems to be an indication of the Belontiidae.
Effects of Di-(2-ethylhexyl) phthalate (DEHP) on Ultrastructure of Rat Testis
Kim, Wan-Jong ; Kil, Young-Chun ; Shin, Kil-Sang ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 353~362
Di-(2-ethylhexyl) phthalate (DEHP) is a plasticize. known as one of endocrine disruptors. The present study was carried out to investigate the ultrastructural changes of prepubertal rat testis after oral administration of DEHP in dosages of 1 g/kg, 3 g/kg or 5g/kg in 0.5 ml of corn oil daily for a week. This study revealed the DEHP inhibited the development of seminiferous tubules and induced structural changes on various cell types of the rat testis. Leydig cells, Sertoli cells and the developing germ cells seemed to be impaired their differentiations in terms of the structural changes of cell organelles. The increase of heterochromatin in amount were common features in all 3 cell types. In addition, the Leydig cells were characterized by the increases in number and size of lysosomes and the scantiness of smooth endoplasmic reticulum. The Sertoli cells became irregular in nuclear envelope and the cytoplasm decreased, but the number of lysosomes and vacuoles seemed to be increased. There were some indications of necrosis of the germ cells, such as vacuolized nucleus and segregated nucleolus. These detrimental effects of DEHP on the rat testis were dose dependent and suppressed spermatogenesis decreasing developing germ cells in number and appearances. The effect of DEHP on ultrastructure of rat testis, as its known physiological functions, seems come from the decreased level of testosterone by Leydig cells, followed by the abnomalities of Sertoli cells and the germ cells.
Effect of Chitosan Oligosaccharide on the Mouse Liver with Toxicated by Carbon Tetrachloride
Hwang, Koo-Yeon ; Yoon, Jung-Sik ; Kim, Young-Ho ; Chung, Min-Ju ; Roh, Young-Bok ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 363~376
This study aims to demonstrate the effect of chitosan oligosaccharide on the ultrastructural changes in the mouse liver toxicated by carbon tetrachloride
. A healthy male ICR mouse that weighted
was used for experiment. The experimental group was divided into three groups; the group A; the pretreated group with chitosan oligosaccharide, the group B; the simultaneous group, the group C; treated only the
. The group A was simultaneously treated with chitosan oligosaccharide and
after pretreated with chitosan oligosaccharide for 7 days. The group B injected
and chitosan oligosaccharide to the intraperitoneal. The group C injected with only
to the intraperitoneal. The results were as follow: In the group A, the nuclear membrane and the mitochondria were observed almost normal in shapes at overall the time. Some lamellae of the RER (rough endoplasmic reticulum) destructed until 48 hours but ribosome attached. The destructed lamellae reformed at 72 hours but the smooth membrane vesicles not observed. The lysosomes observed at 72 hours. At 96 hours, all organelles showed in normal shapes. In the group B, changes of nuclear membranes were relatively lighter than group C. Mitochondria observed normal shape through the time. Parts of RER reformed the lamellae, other parts dilated inner cavity. And lipid droplet observed around the 24 hours. Glycogen and lysosome observed 48 hours and 72 hours, respectively. In the group C, nuclear membrane was irregular and nuclear cytoplasm condensed through the time. The lamellae of RER destructed from 24 to 96 hours. Smooth membrane vesicles observed in the cytoplasm at 48 ours. Mitochondria was less effected by toxic. And from the 24 hours, the variable sizes of lipid droplets observed in tile cytoplasm. These results suggest that chitosan oligosaccharide attenuates the toxic effect of the carbon tetrachloride in the mouse liver.
Time Course of Fibular Osteoporosis in Ovariectomized Rats
Bae, Chun-Sik ; Park, Chang-Hyun ; Uhm, Chang-Sub ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 377~382
Osteoporosis means a deficiency in the amount of bone tissue in the skeleton or part of the skeleton. Osteoporosis is a lesion, not a specific disease. 'Osteoporotic' describes the slate of a bone or skeleton at a given time. Osteoporosis may be diagnosed subjectively by visual appraisal, or objectively by measurement of radiographs, sawn bones, or microscopic sections. This study was carried out to make clear of the influence of ovariectomy on time course of fibular osteoporosis in rats. Seven weeks after ovariectomy, osteoporosis was evident, when the size of the bone marrow cavities significantly increased and the width from the bone marrow cavity and cortex significantly decreased than normal.
Immunohistochemical and Ultrastructural Characterization of the Choline Acetyltransferase-immunoreactive Nerve Cells in the Diagonal Band of Broca of the Rat Basal Forebrains
Back, Seung-Keun ; Chung, Young-Wha ;
Applied Microscopy, volume 29, issue 3, 1999, Pages 383~403
This study was performed to investigate the immunohistochemical and ultrastructural characterization of the choline acetyltransferase (ChAT)-immunoreactive nerve cells in the diagonal band of Broca of the rat basal forebrains, utilizing techniques of immunohistochemical and immunocytochemical microscopy. The ChAT-immunoreactivities were shown within neuronal cell bodies and processes by the light micoscope. According to cell shape and ratio of long axis vs short axis of cell body, the ChAT-immunoreaclive nerve cells in both vertical and horizontal limbs of the diagonal band of Broca were classified into 6 types. at the light microscopic level; round, oval, elongated, fusiform, triangular and polygonal types. As a result of the electron microscopic observation, the ChAT-immunoreactivated products appeared on the outer nuclear envelope, membranes of rough endoplasmic reticula (rER), free ribosomes and polysomes. Each cell type was subdivided into subtype I and II according to the several criteria such as volume of cell body, nuclear size relative to the cytoplasm, kinds and distribution of cell organelles and numbers and sorts of synapses. The subtype I of immnunoreactive nerve cells had large cell body and a small nucleus showing shallow indentations of nuclear evelope. In this subtype I with abundant cytoplasm, rER were well differentiated. Their long cisternae were parallelly ditributed and lamellated. One or two lamellar bodies and nematosomes were observed. The subtype II cell had small cell body and a large nucleus with deep indentations of nuclear envelope. In this subtype II with small cytoplasm, the rER were irregularly distributed and the lamellar body and nematosome were not found. A few axosomatic synapses in the subtype I and II were shown to be symmetric or asymmetric. The ratios of the symmetric synapse to the asymmetric one were investigated to be 1 : 2 and 1 : 4 in the subtype I and II, respectively. The axodendritic ones were almost asymmetric. But, the fusiform and triangular immunoreactive nerve cells were shown only to be subtype I. According to observations in this study, it is considered that the ultrastructural characterization in the 2 subtypes of each cell type may reflect the differences of the metabolic activities and projecting distances to the target cells.