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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 3, Issue 1 - Dec 1973
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Ultrastructural Localization of Acid Phosphatase in Entamoeba histolytica and Entamoeba gingivalis
Cho, Kee-Mok ; Cha, Hai-Young ; Soh, Chin-Thack ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 1~16
A combined cytochemical and electron microscopic study was carried out for the demonstration of acid phosphatase activities in trophozoites of E. histolytica. and E. gingivalis. E. histolytica(YS-27) strain was isolated from liver abscess of 72-year-old man in September 1969, and E. gingivalis (YS-215) strain was collected from gingival crevice of 41-year-old man in January 1972. The amoeba strains were maintained by subculture on diphasic medium, and used throughout the study. The results are summarized as follows; 1. In E. histolytica, the reaction products were distributed evenly over the entire surface of plasma membrane, whereas E. gingivalis showed no activity of acid phosphatase on the plasma membrane, except in the portion of the uroid-like structure. 2. In the cytoplasm, various reaction precipitates were observed in vacuoles of both amoebae; vacuole limiting membrane, vacuole membrane and its contents and lysosome-like structure. Strong enzyme active contents but membrane reaction negative vacuoles were conspicuous in E. gingivalis. Endoplasmic reticulum showed a moderate activity. 3. Granule-like acid phosphatase reaction product was demonstrated in the nucleoplasm of E. gingivalis, but it was negative in E. histolytica.
Changes of Endoplasmic Reticulum of Rat Intestinal Epithelium Induced by Ethionine Feeding
Kim Poong-Taek ; Sohn Tae-Joong ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 17~22
The author studied ed the effect of ethionine upon the absorption epithelium of ileum with particular ettention to the endoplasmic reticulum. Five tenth per cent of DL-ethionine was added to the diet of the experimental group rats and they were sacrificed after 1, 2, 3, and 4 weeks. respectively. The ileum were observed by the electron microscope. The results obtained were summarized as follow: The changes were detachment of membrane bound ribosome, dilatation of endoplasmic reticulum, decrease of polysome with reciprocal increase of monosome, and dilatation of Golgi complex. These changes were occured at 2 nd to 3 rd week from start of ethionine feeding and more severe at fouth week. These observation suggested that ethionine induced degenerative changes of the organelles.
The Changes of Rough Surfaced Endoplasmic Reticulm of Lymph Node Cells Stimulated by Antigen
Lee Chang-Soo ; Kwon Sae-Hoon ; Sohn Tae-Joong ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 23~28
The changes of rough surfaced endoplasmic reticulum of plasma cells in the regional lymph node after injection of Bovine Gamma Globulin summarized as follow: The rough ER of plasma cells before antigenic stimulation shows flattened sac but changed to vesicular at 3rd day and vacuolar at 7th to 10th day. lntradisternal granules are appeared within the lumina or rough ER at 7th to 10th day. The rough ER is changed to distended sac or flattened sac at 20th day. The results suggest that normally flattened sac of rough ER is changed to vesicular and then to vacuolar after antigenic stimulation. But they are returned to distended sac or flattened sac with disappearance of antigenic stimuli.
Electron Microscopic Studies of Human Keloid and Hypertrophic Scars
Kim, Chung-Soak ; Lew, Jae-Duk ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 29~38
Introduction. The human cutaneous scars manifest themselves many ways in different types according to the factors such as the age, sex, race of the patient as well as the location,. kind and heal ing process of the wound. Among the scars it is quiet difficult to verify the clinical course of the hypertrophic or keloidal scars from the true keloids. However, clinical observations indicate that stress, either mechanical or in the forms of chronic infections, can induce a functional change in the fibroblasts causing an excessive production of collagenous matrix. In this study, we preliminary attempt to justify any difference of the cellular structure between keloids and hypertrophic scars by using electron microscope. Material and Methods. A total of 23 cases: 2 scars, 2 hypertrophic scars and 19 keloids are examined. Immediately, the biopsy tissue was fixed in 10% neutral formalin and 4% glutaraldehyde solution in phosphate buffer for 4 hours, post fixed in 1 % osmium tetraoxide for two hours, dehydrated with graded alcohol, and embedded in Epon 812. Thick sections were stained with hematoxylin eosin, periodic acid-Schiff(PAS) and Van Gieson stain. Thin sections were cut and uranyle acetate, lead citratestain and examined with the electron microscope. Result. The morphologic features of keloid showed thick, homogenously eosinophilic bands of collagen and numberous large active fibroblasts. The hypertrophic scar and soft scar are more cellular than keloid and composed thinner collagenous fiber. For this paper in the etiology of keloids can not as be defined, but and interesting keloidal tissue fibroblast showed irregular nucleus with irregular shape dense bodies and fibril materials contained in to the cytoplasm.
Electron Microscopic Studies on the Rat Mast Cells Induced by Morphine Hydrochloride
Kang, Ho-Suck ; Kim, Chang-Whan ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 39~44
The effects of morphine HCl on the mesenteric mast cells were studied the electron microscopy. The results of the observations are as follows: 1. In the experimental group for intravenous injection of morphine HCl 12 mg/kg, the granules appeared cluster, granular lysis and an electron transparent appearance. Frequently, some granules appeared in the extracellular space. 2. In the experimental group for intravenous injection of morphine HCl 24 mg / kg, it was observed. that the formation of a clear halo or a space around each granule. Many altered granules showing a reticular texture (type 2) are observed in the cytoplasm. 3. From the results mentioned above, it is suggested that rat mesenteric mast cell' granules were affected by morphine HCl.
Electron Microscopic Observations of Mouse Liver Cell Treated with Fungal Culture Filtrates Isolated from Foodstuffs II. Results of Isolated Strains
Deung, Young-Kun ; Choi, Choon-Keun ; Koh, Choon-Myung ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 45~54
The present study is to determine the toxicity of the fungi isolated from foodstuffs by observing the ultrastructural changes in the mouse liver cells. The results as follows: 1. The toxin-producing fungi were screened by the methods of toxin-screening test(cyto-toxicity test against to HeLa cells and thin layer chromatography). 2. All of the experimental animals treated with isolated fungi were observed the focal necrosis and inflammatory infiltration of liver parenchymal cells. 3. It showed the cytoplasmic changes, such as dilatation of rough endoplasmic reticulum (RER), swelling of mitochondria (mi). increased number of lipid droplet (li) and glycogen (gl), detachment of ribosomes (ri) by observing the electron microscopy. 4. Nuclear and nucleolar alteration were also noted the segregation of nucleolar element and irregularity of nuclear envelopes. 5. As a mass screening, the cytotoxicity test using HeLa cells and thin layer chromatography are feasible methods to detection of the mycotoxin producing fungi from various sources.
Electron Microscopic Studies On The Kinetoplasts In Trypanosoma
Inoki, Shozo ;
Applied Microscopy, volume 3, issue 1, 1973, Pages 55~59