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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 31, Issue 4 - Dec 2001
Volume 31, Issue 3 - Sep 2001
Volume 31, Issue 2 - Jun 2001
Volume 31, Issue 1 - Mar 2001
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Effects of Safflower (Carthamus tinctorius L.) Seed Powder on Bone Resorption in Ovariectomized Rats
Bae, Chun-Sik ; Park, Chang-Hyun ; Chang, Byung-Joon ; Kim, Hwi-Yool ; Cho, Ick-Hyun ; Uhm, Chang-Sub ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 109~116
Safflower has been cultivated in Korea and thought to have excellent effects on bone in oriental medicine and folk remedy and has been taken for a long time. Safflower is thought to be helpful for the development and sustenance of bones according to the result of recent assay of its components. Otherwise, any reliable experimental data have not been suggested so far. We have carried out this study to examine the prophylactic effects of safflower-seed-powder on the prevention of osteoporosis induced by the ovariectomy 12 Weeks-old Sprague-Dawley rats weighing about 230 g was kept in the experimental condition and used in this study. Animals were taken 0.3 g of safflower-seed-powder once a day for 1, 3, 5, and 7 weeks after ovariectomizing both ovaries and observed the fine structure of tibia. Tissues were fixed with traditional SEM preparation methods and decalcified for 10 hours with 10% nitric acid and dehydration, drying, and gold-coating were followed by the routine procedures and observed with scanning electron microscope (Hitachi, S-450). Loss of bone was started just after ovariectomy and thickness of bone from the medullary cavity to the compact bone was reduced and the extension of medullary cavity was serious in the control group of 7 weeks. Experimental groups taken safflower-seed-powder showed similar findings from 1 week to 7 weeks. These results suggest that the safflower-seed-powder is thought to be efficient for the prevention of osteoporosis owing to the deficiency of female sex hormone.
A Scanning Electron Microscopic Study on the Phase Demixing of PVA Gel
Hong, Sung-Goo ; Sohn, Jeong-In ; Lee, Ihn-Chong ; Kim, Soo-Jin ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 117~128
DMF is not a good solvent for PVA. There is no solvent-PVA interaction such as H-bonding. DMF/PVA makes a UCST system. DMF/PVA makes a gel through crystallization-induced gelation. X-ray, thermal analysis, and other experimental proofs are presented. The gelation rate was faster at low temperature. Small addition of PEG increased the rate of gelation, but urea decreased the rate. SEM showed the phase demixing process very clearly. In the early stage of gelation, only phase demixing was occurring at a low rate. Hence, no holes appear in the early stage photographs. As demixing proceeded further, the holes began to appear and the sizes became bigger. DMF phase remains many holes after vaporization and PVA phase constitute the matrix phase.
Spermiogenesis in the Saghalien Pygmy Shrew, Sorex minutus gracillimus
Heo, Jin-Chol ; Lee, Jung-Hun ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 129~141
To investigate the spermiogenesis of the Saghalien Pygmy shrew (Sorex minutus gracillimus), the testis obtained from mature male shrew was studied by electron microscopy, and the following results obtained based on the morphological characteristics of cell differentiation of the seminiferous epithelium in the testis. According to the fine structural differentiation, spermiogenesis of S. minutus gracillimks was divided into Golgi, cap, acrosome, maturation and spermiation phases. Beside, the Golgi and cap phases were subdivided into three steps of early, middle and late phase respectively, and acrosome phase into two steps of early and late phase , and maturation and spermiation phases has only one step respectively. Thus, the spermiogenesis of S. minutus gracillimus was divided into a total of ten steps. The chromatin granules begin to be condensed in the acrosome phase, and a perfect nucleus of sperm was formed at the spermiation phase. Mancette were appeared from the late acrosome phase to the maturation phase. The formation of sperm tail began to develop in the late Golgi phase, and completed at the spermiation phase. Multivesicular bodies were appeared from the Golgi phase to the maturation phase, recognized with pale, pale and moderate, and dense at Golgi, cap and acrosomal and matulation phases respectively.
Differentiation of Seminiferous Epithelium and Spermiogenesis in the Testis of Rana catesbeiana
Go, Song-Haang ; Lee, Jung-Hun ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 143~156
The aim of this study was to clarify the differentiation of seminiferous epithelial cells and spermiogenesis in the testis of Rana catesbeiana. Spermatogenesis of R. catesbeiana consists of primary spermatogonia, secondary spermatogonia, primary spermatocytes, secondary spermatocytes and spermatids. They were subdivided into eight stages on the basis of the morphological features of the germ cell differentiation. From the spermatocytes except primary spermatogonia to before the spermiation of spermatids were surrounded by spermatocyst. Spermiogenesis of R. catesbeiana can also be divided into three stages on the basis of morphological features of the nucleus and the cytoplasm organelles. Spermatozoon contained a saccular acrosome, a cylindrical and tapered slighty at both ends head, and a tail with only the axoneme.
An Ultrastructural Study of Sertoli Cells in Human Fetal Testes
Lee, Tae-Jin ; Yoon, Sam-Hyun ; Kim, Mi-Kyung ; Park, Eon-Sub ; Yoo, Jae-Hyung ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 157~165
Sertoli cells in the normal adult testis are nondividing cells, which are relatively inconspicuous on cross section of the seminiferous tubule and comprise about 10% to 15% of the tubular cellular elements. Ultrastructurally, Sertoli cells have characteristic nucleoli, plasma membrane, and cytoplasmic components. The plasma membrane has two types of intercellular junctions which are developed at puberty: junctions between adjacent Sertoli cells and Sertoli cell-germ ceil junction. However, the ultrastructural findings of Sertoli cells in human fetus is not fully elucidate yet. In the present study, human fetal testes (
weeks) obtained from artificially induced abortions legally without gross malformation were studied using transmission electron microscopy to make clear the differentiation process of Sertoli cells in human. In human fetal testes from 14 weeks to 27 weeks, the cell junctions of Sertoli-germ cells and Sertoli-Sertoli cells are desmosome like structure and not tight junction or desmosome. The Overall intracytoplasmic organelles of Sertoli cells are relatively sparse. The mitochondrias are relatively abundant but no developed cristae. And the rough endoplasmic reticuli are abundant and smooth endoplasmic reticuli are sparse. The amount of lipid droplets are regularly observed in human fetal Sertoli cells. No microfilaments or Charcot-Bottcher's crystalloids are present. From the results, Sertoli cells in human fetal testes are somewhat different ultrastructural findings with puberty or adult. However, to make clear the differentiation process of Sertoli cells in human, further study for 28 weeks to puberty is required.
Ultramicroscopical Study on the Astrocytes in Fetal and Neonatal Brains of Korean Native Goat
Song, Chi-Won ; Kim, Moo-Kang ; Ryu, Si-Yoon ; Lee, Kang-Lee ; Park, Il-Kwon ; Lee, Mi-Young ; Kwon, Hyo-Jung ; Park, Mi-Sun ; Lee, Kyoung-Youl ; Lee, Doo-Hwan ; Shin, Hyun-Guk ; Choi, Yoon-Suk ; Chang, Kyu-Tae ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 167~173
This study, the ultrastructure of radial glial cells in the fetuses (the 60th, 90th, 105th 120th of gestation) and neonate brains of korean native goat were investigated by immunohistochemical method and transmisson electron microscopy. 1. In the 60th day of gestation, mitochondria and many glycogen body were observed in the radial glial cells. 2. In the 90th day of gestation, mitochondria, many glycogen body and rough endoplasmic reticulum were observed. 3. In the 95th day of gestation, Golgi's apparatus was found. 4. In the 120th day of gestation, Endfeets of radial glial cell attached vessel wall were observed.
Effects of Chitosan on the Mouse Hepatotoxicity Toxicated by Mercury
Roh, Young-Bok ; Yoon, Jung-Sik ; Chung, Kyung-Ah ; Chung, Min-Ju ; Kim, Young-Ho ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 175~184
This study aims demonstrate the effect of chitosan, one of the natural chelator, on the ultrastructural changes in the mouse liver caused by
. The experimental group was divided in two groups; group A and group B. The group A administrated
(5.0 mg/kg) to the oral. The group B treated with
(5.0 mg/kg) and chitosan (3%) solution, 2 times/day). Each group was observed 24, 48, 72 and 96 hours after treated
and chitosan. Histological changes of the livers were investigated by electron microscope. 1. Croup A Nuclear membrane was shrinked. The inner and outer membrane of the mitochondria were dilated. Destruction of lamellae of rough endoplasmic reticulum showed. Smooth endoplasmic reticulum showed over cytoplasm. 2. Group B Nuclear membrane was more rounded, The cristae of the mitochondria were almost normal shape and electron-density showed compacted. Dilation of inner cavity of rough endoplasmic reticulum showed at the pre-time but formed typical lamellae at the 48Hrs. Smooth endoplasmic reticulum showed over cytoplasm. Therefore, we concluded that chitosan has significantly protective effects in liver to harmful
The Fixation Effects in Immunohistochemistry and Electron Microscopy Using Low Energy of Microwave (LEM) in Human Gastric Adenocarcinoma and HeLa Cell
Yang, Seung-Ha ; Son, Tae-Ho ; Shin, Kil-Sang ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 185~197
Human gastric adenocarcinomas are fixated with low energy of microwave (LEM) to study fixation effects in level of ultrastructure and antigenicity of the cancer. For the Ag-Ab reactions , the LEM fixated sdenocarcinomas are incorporated with monoclonal mouse anti-human p53 (IgG2b, kappa) and rabbit anti human cerbB-2. The retrieval of antigenicity are easily recognizable in the LEM fixated sections compared with that of frozen sections which show often diffused colour reactions. And the LEM fixation methods have preserved ultrastructures of the adenocarcinoma, but it was often difficult to maintain constancy in fixation effects. For the constancy, LEM was coupled with low concentration of chemical fixatives, such as glutaraldehyde (<1%) and
(<0.5%). The results were acceptable, but there are tendencies that the adenocarcinoma requisitioned rather weak microwave energy to come into the optimal fixation effects. Therefore , cultured HeLa cells were fixated with lower energy of microwave than that used to the adenocarcinoma. The ultrastructures of the single HeLa cell have been preserved. The results may imply that a different energy levels of microwave are requisitioned in accordance with kinds of cells and tissues for the optimal fixation effects. It is reported and discussed that the fixation methods of LEM used in this work could be applied routinely to conceal a insufficient diffusion rate of chemical fixatives into some kinds of cancer without compromising the ultrastructures as well as to improve antigenic quality of frozen sections.
Immunocytochemical Investigation on the Intracisternal Accumulations of Storage Protein in Pea Cotyledon Cells
Jeong, Byung-Kap ; Park, Hong-Duok ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 199~206
In 1980s, the fragmentation or subdivision of protein deposits at the periphery of protein storage vacuole was suggested as the only route of PB development in pea cotyledon cells. Since then, other independant processes such as terminal dilation , transformation and de novo development have been discussed as alternative routes for PB development, and today, these multiple mechanisms of PB development are accepted as a result of active investigations. For analysis of the protein accumulations in the ER cisternae during seed development, immunocytochemical gold labellings were applyed on the single cells separated by enzymatic digestion from cotyledon tissue. Anti-legumin labellings at the early stage, and anti-vicilin labellings at the intermediate stage were observed on the protein-filled ER. The
, which is the ER retention protein, was labelled somewhat at late stage, and PPase, a sort of tonoplast membrane protein, was labelled at early stage.
Modified Kranz Structure in Leaves of Salsola collina
Kim, In-Sun ;
Applied Microscopy, volume 31, issue 2, 2001, Pages 207~214
Anatomy and ultrastructure of the modifeid Krana pattern have been studied in succulent Salsola collina Pall. Cylindrical leaves exhibited the Salsoloid Kranz type containing two layers of peripheral chlorenchyma that surrounded the water storage cells and vascular tissues. Small veins were also peripherally arranged, but mostly embedded in the vicinity of the inner chlorenchma without the orderly arrangement of the concentric layering of bundle sheath and mesophyll cells. The current study mainly focused on the chlorenchyma tissue abutting such minor veins. The outer columnar layer exhibited features similar to the characteristics of palisade mesophyll cells, while the inner cuboid layer to the bundle sheath cells of a typical
Kranz pattern. Cellular components of the inner chlorenchyma were centripetal and numerous, but starch-laden chloroplasts were rudimentary in the thylakoidal system. The outer chlorenchyma demonstrated normally developed chloroplasts having well-stacked thylakoids and plastoglobuli. Branched and complicated plasmodesmata frequently occurred in thick interfaces of the two layers, implying the active movement of the photosynthates between them. The present data were mostly congruent with one of the structural features of the C4 subtypes , NADP-ME type, reported in the
pattern. The Kranz pattern encountered in this Salsola probably has been directly related to the structural modification that occurred during a functional adaptation to the