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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 31, Issue 4 - Dec 2001
Volume 31, Issue 3 - Sep 2001
Volume 31, Issue 2 - Jun 2001
Volume 31, Issue 1 - Mar 2001
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Broad Bean Wilt Fabaviruses and Their Specific Ultrastructures
Choi, Hong-Soo ; Choi, Jeom-Deog ; Lee, Keum-Hee ; Kim, Jeong-Soo ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 215~222
Pathogenicities of the five BBWV isolates were differentiated by the reactions on the 29 host plants including Chenopodium amaranticolor. Three specific ultrastructures were observed in cells infected with BBWV The first ultrastructure was the tube made of
layers of virus particles. The second one was the comb structure consists of round and angled structures. The last one was the membrane proliferation in the cytosol.
Fine Structure on the Spermiogenesis of Octopus minor on the Western Coast of Korea I
Chang, Nam-Sub ; Kim, Sang-Won ; Han, Jong-Min ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 223~233
The spermiogenesis of a Korean octopus, Octopus minor, inhabiting western of Korea Sea was observed by electron microscopy . The obtained results are as follows: The spermiogenesis of Octopus miner proceeds through four stages; early- , mid- , and late-spermatid, and mature sperm. An early spermatid is a spherical cell looking light due to the low electron density. The acrosome formed from Golgi complex of the upper nucleus looks dark due to the high electron density. The extra-nuclear rod (enr) stemming from proximal centriole is transformed from round shape into oval shape, elongating to the upper nucleus. In our observation, the axoneme was being formed from distal centriole, and the manchette composed of a number of microtubules is also found around nuclear membrane. In a mid-spermatid, chromatins in the nucleus contract shaping fine threads, and the manchette is also observed around nuclear membrane. Especially, the spherical acrosome is transformed into long oval one which is tinged with a number of horizontal stripes and has the middle electron density. In a late-spermatid, chromatins in the nucleus contract thick and short. Furthermore, the mitochondrial sleeve, in which the axoneme is surrounded with mitochondria, is observed at middle piece. The axoneme has a typical structure of 9+2 and around it, 9 coarse fibers are observed. Also in the acrosome cavity of mature sperm, horizontal striation is found. However, regularly spaced processes are peculiarly observed in there. A sperm is about 390 fm long, whose head is bent a little like a banana while the acrosome region is helical. In the middle piece of sperm,
mitochondria are surrounding coarse fibers that reach the main piece of tail, while nothing but 9+2 structured axoneme is found in the end piece.
Scanning Electron Microscopic Observation of Trichomonas vaginalis Contacted with Human Vaginal Epithelial Cells
Kim, Seung-Ryong ; Ryu, Jae-Sook ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 235~244
The aim of this study was to observe morphological changes of Trichomonas vaginalis after contact with human vaginal epithelial cells, by scanning electron microscope. The vaginal epithelial cells (VEC) (menstrual, days
) from normal women were mixed with T. vaginalis (VEC/trichomonads ratio of 1 : 10), and incubated for 30 min. The parasitic body was changed to a more elongated shape with pseudopodia or was flattened to ameboid transformation showing highly adherence to VEC.
Fine Structure of the Spermatogenic Cells during the Spermiogenesis of Paradoxornis webbiana
Lee, Jung-Hun ; Hahm, Kyu-Hwang ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 245~256
The morphological characteristics of spermatogenic cells during the spermiogenesis of Paradoxornis webbiana were studied by transmission electron microscope. Spermiogenesis of P. webbiana was divided into ten phase. The chromatin granules became fibrous granules at the Golgi phase, gradually condensed at the cap phases, condensed as a stick at the acrosomal phase, and finally, a perfect nucleus was formed at the maturation phase. The formation of sperm tail began at the early Golgi phase, and completed at the late maturation phase. In particular, the dense materials existed in the sperm neck, which is wedged between the tip of segmented columns and the first mitochondria of the middle piece. The axone in the neck were surrounded by the dense materials. The axonema in spermatozoon contains a 9+2 arrangement of microtubules: 9 doublets, and 2 central single microtubules. Mitochondrial bundles of middle piece were composed of a pair of arms, which surrounded the axone of the middle piece by the
angled-helical structure. The outer membrane of mitochondria were surrounded by microtubules in plasma membrane of the sperm. The undulating membrane had a helical structure, and the sperm plasma membrane was surrounded by undulating membrane.
Glycogen Effect of the Sperm Maturation during the Spermiogenesis of Rana catesbeiana
Go, Song-Haang ; Lee, Jung-Hun ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 257~266
To investigate the process of spermoigenesis and glycogen effect during the spermatogenesis of Rana catesbeiana, the morphological characteristics of the testes were examined by light and transmission electron microscopy. Spermiogenesis of R. catesbeiana was divided into three stages on the basis of the features of the nucleus and the cytoplasm organelles. Except for the primary spermatogonia, the phases from the spermatocytogenesis to the spermatids before spermiation phase were surrounded by spermatocyst. Especially , the glycogen particles were not observed until in the stage of spermatocytogenesis, but from the early spermatids to the maturation phase were observed in the nucleus, acrosome and cytoplasm of the spermatids. The present result suggests that the glycogen may play an important role in the sperm maturation, and as a source of the energy in the wave-movement of sperm tail.
Effects of Saengchinyanghyoltang-gamibang on the Pancreatic Islets of the Mice Induced with Streptozotocin
Kim, Jeong-Sang ; Park, Min-Hee ; Jung, Hae-Man ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 267~274
This study was carried out to understand the effects of Saengchinyanghyoltang-gamibang (SGT) on pancreatic islets of the mice induced with streptozotocin (STZ). In the control group, two times injected with 50 mg/kg 572 at 24-hour intervals, a few number of insulin immunoreactive-cells are observed at the pancreatic islets of the mice. In the experimental group which administered with extract of SGT during 21-day, a number of immunoreactive-cells are observed at the pancreatic islets. According to the electron microscopic observation,
of the control group were contained a few of secretory granules, but also these granules were contained electro-lucent materials. In the experimental group, a lot o( secretory granules and well developed cell organelles are observed at the
. The level of glucose was significantly decreased in the experimental group compared with control group, but the level of BUN was similar in these two groups. These results suggest that administration of SGT to the mice improved the damage of
from injected with STZ.
Increase of Alveolar Macrophages Contributes to the Enhanced Xanthine Oxidase Activity in the Bronchoalveolar Lavage Fluid of Rats Given IL-1 Intratracheally
Cho, Hyun-Gug ; Yoon, Chong-Guk ; Choi, Jeung-Mok ; Park, Won-Hark ; Lee, Young-Man ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 275~285
The pulmonary alveolar macrophage is thought to play an important role in the mediation of acute inflammatory lung injury by secretory products including degraded enzymes, cytokines, and reactive oxygen metabolites . This study was conceived to understand the role of alveolar macrophage in oxidative stress induced acute lung injury. To examine the alveolar macrophages and xanthine oxidase (XO) activity in bronchoalveolar lavage fluid (BALF), time-dependent changes of numbers of alveolar macrophages, monocytes and neutrophils in alveolar cavity were counted in association with ultrastructural and cytochemical observations of lung tissue and alveolar cells. The number of monocytes was increased (p<0.001) at 1h after IL-1 treatment compared with that of sham. At 2h after instillation of IL-1, the number of alveolar macrophages was the highest, XO activity in BALF was elevated at 2h after IL-1 instillation and the activity was markedly elevated(p<0.05) at 3h after IL-1 treatment. On the basis of these experimental results, it is suggested that, during early phase of acute lung injury induced by IL-1, alveolar macrophage-derived XO contributes to lung injury earlier than the neutrophilic respiratory burst.
The Role of Cartilage Canals in Osteogenesis and Growth of the Vertebrae in the Human Fetuses
Jung, Sung-Taek ; Nam, Kwang-Il ; Kim, Baik-Yoon ; Yoon, Jae-Rhyong ;
Applied Microscopy, volume 31, issue 3, 2001, Pages 287~305
To investigate a role of cartilage canals in osteogenesis and growth of the vertebrae, in human fetuses ranging from 50 mm to 260 mm crown rump length were studied by electron microscopy. The initial appearance of cartilage canals of the vertebral body was observed at 60 mm fetus. In 80 mm fetus, primary ossification center in the vertebral body was first noted. The vertebral body showed calcified chondrocytes surrounded by a tone of hypertrophied chondrocytes and deep canals which terminated in calcified matrix. Most hypertrophied chondrocytes in the centrum showed in various stage of degeneration in disorderly arrangement. At the blind end of deep canal, osteogenic cells, osteoblasts and chondroclasts were observed. Resorption of unmineralized cartilage septa was undertaken by perivascular cells within cartilage canals. The ruffled border of the chondroclast was restricted to resorption site of calcified cartilagenous matrix. The periosteal bone formation was followed by the appearance of primary center of the centrum at 120 mm fetus. The osteoblasts of the perichondrium started to lay down a thin membranous bony lamella on the outer surface of the osseous trabeculae of the centrum. The processes of bone formation in the vertebral bodies were found to possess morphological similarities to that occurring at secondary center of the epiphysis of a long bone. These results indicate that the connective tissue cells within the cartilage canals proliferate and differentiate into osteoblasts at the site of endochondral ossification of the vertebrae.