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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 31, Issue 4 - Dec 2001
Volume 31, Issue 3 - Sep 2001
Volume 31, Issue 2 - Jun 2001
Volume 31, Issue 1 - Mar 2001
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Effects of Safflower (Carthamus tinctorius L.) Seed Powder on Fracture Healing in Rats
Park, Chang-Hyun ; Uhm, Chang-Sub ; Bae, Chun-Sik ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 307~314
Safflower (Carthamus tinctorius L.) is a thistle-like annual plant mainly grown in dry hot climates as an oilseed or birdseed. Traditionally, the oil has mainly been sold in the health food market because it is unsaturated having high linoleic and oleic acid levels. With increased health consciousness in recent years, the oil quality has become a more general health issue. This study was designed to understand whether safflower seed powder has positive effects on the fracture healing in rats. Simple transverse fracture of rat fibula was made with a rotating diamond disc saw. The histologic changes of rats were observed with a scanning electron microscope. The fractured fibulae showed a complete fusion at the fracture site in the 4th to 5th week after a simple transverse fracture. Administration of safflower seed powder facilitated the speed of histologic changes without affecting qualitative changes. These results suggest that safflower seed powder nay have substances that help the fracture healing process.
An Investigation of In Situ TEM Heating Experiments of Powder Samples
Kim, Youn-Joong ; Jeung, Jong-Man ; Lee, Young-Boo ; Lee, Su-Jeong ; Song, Ji-Ho ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 315~323
In situ TEM heating experiments utilizing kaolinite powder samples result in the following facts. (1) The water recirculation system adopted in the Gatan's heating holder is required to prevent specimen drift above
. (2) Since the degree of phase changes depends on the thickness of powders below
, examinations of both thin and thick specimens are required. (3) Sample preparation using Mo-grids is required for TEM heating experiments above
. At these temperature ranges the effect of heating rate and holding time on the phase transition process increases drastically, so that a programmed temperature control is required. (4) TEM heating experiments of the embedded powders by epoxy for the cross-sectional view was limited due to the severe epoxy movement during heating above
. Better methods of sample preparation are required to overcome this problem.
Ultrastructure and Histochemistry on the Integumentary System of the Stone Flounder, Kareius bicoloratus (Teleostei: Pleuronectidae)
Lee, Jung-Sick ; Jin, Young-Guk ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 325~331
Integumentary structures of the stone flounder, Karefus bicoloratus were examined by means of the light and transmission electron microscopy. Stratified epidermal layer consists of supporting cells, unicellular glands and granular cells. The epidermal layer could be classified into superficial, intermediated and basal layer by morphology and structure of the supporting cells . The cytoplasm of supporting cells is divided into cortex and medullar part. In the cortex microfilaments are well developed. Mucous cells of unicellular gland were observed in the superficial and intermediated layer of the epidermis. The mucous materials were identified as glycoprotein of neutral and carboxylated mucosubstance by histochemical methods. Club cell has well developed smooth endoplasmic reticula and Golgi complex in the cytoplasm. Granular cells were observed in the intermediated and basal layer, and the cytoplasm is occupied with membrane-bounded granules of electron dense. Three types of pigment cells could be distinguished with electron density of cytoplasmic inclusions. Nerve myelins were observed near the pigment cells.
Fine Structure of Mature Sperms of Cephalopods (Octopus minor, Octopus ocellatus and Todarodes pacificus) Inhabiting the Korean Waters II
Kim, Sang-Won ; Chang, Nam-Sub ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 333~345
The mature sperms of three species of cephalopods (Octopus minar, Octopus ocellatus, Todarodes pacificus) were observed by electron microscopy. The results obtained are as follows: The sperm lengths of Octopus minor and Octopus ocellatus of octopods are long and they are about
, respectively, but the sperm length of Todarodes pacificus is short and about
. The sperm of Octopus minor has a helical acrosome and a head bent a little like a banana while Octopus ocellatus of octopod has a twisted acrosome and a long rod-shaped head. A number of horizontal stripes are observed as a periodic structure in their subacrosome cavities and dense plugs are formed in the cavities of their heads. On the other hand, the acrosome of Todarodes pacificus is circular cap-shaped, and its head is long and oval. It is notable that two small cavities were observed in its basal acrosome. Juxtanuclear acrosomal materials of high electron density filled the subacrosomal cavity. In the middle piece of mature sperms of Octopus minor and Octopus ocellatus, the mitochondria form the mitochondrial sleeve, but the numbers of mitochondria differ between the species so that they are
, respectively. Meanwhile, in the middle piece of mature sperms of Todarodes pacificus, the mitochondria are separated from the axoneme, forming a mitochondrial spur in which
mitochondria and some electron dense materials concentrate. The axoneme of Octopus minor, Octopus ocellatus and Todarodes pacificus are of 9+2 type in common, surrounded by 9 coarse fibres. A number of glycogen were observed only in the axoneme of Todarodes pacificus. The coarse fibres were found as far as the main piece of sperm tail in Octopks minor and Todarodes pacificus, while to the end piece of sperm tail in Octopus ocellatus.
Legumin Accumulation in Endoplasmic Reticulum Cisternae at Early Stage of Seed Development and Protein Body Transformation in Pea Cotyledon Cells
Jeong, Byung-Kap ; Lee, Sun-Hee ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 347~354
Immunoelectron microscopy of storage protein at early stage of seed development showed legumin was firstly accumulated protein in between endoplasmic reticulum (ER) cisternae, and these accumulates were differentiated into protein body (PB) by transformation at later stage. Thin sections of pea cotyledons during the later stages of seed maturation showed three morphologically different types of protein bodies. One of these, presented as rough-surfaced cisternae with terminal dilations, which contained protein deposits and were often found interdigitated between stacks of rough endoplasmic reticulum. Conventional electron microscopy at earlier stages of cotyledon development showed this protein body type initially developed from the rough ER. This transformation of endoplasmic reticulum into a protein body is believed to represent a new pathway of protein body development.
The Differentiation of the Cholinergic Nerve Cells at the Meynert Basal Nucleus of the Basal Forebrains in the Growth Period of Rat
Hahm, Young-Ok ; Kim, Soo-Jin ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 355~365
This study was performed to investigate the distribution and differentiation on the immunoreacted cells of the ChAT (choline acetyltransferase) at the Meynert basal nucleus of the forebrains in the growth periods of rat, using the immunohistochemistric method. According to the cell shape and the ratio of long axis vs short axis of cell soma, the ChAT antibody reacted nerve cells in the Meynert basal nucleus of the rats were classified into six types. In the adult rats, the FD (frequency distributions) of round, oval and elongated cells were maximum. The FD of these types were shown to be progressively decreased during the postnatal development. In addition, the FD of elongated nerve cells in were observed in the adult rats respectively. This was thought to be the same phenomenon as those in the round and oval cells . The total mean volume of ChAT antibody reacted cell somata was lowest in the PND (postnatal days) 7 rats and was highest in the PND 21 rats. But, those were decreased to the adult. These results suggest that ChAT antibody reacted nerve cells grow up to PND 21 and then, differentiate into the various types by neurites outgrowing. On the electron micrography, the adult rat forebrain cells were obtained to be well developed ribosomes, polysomes , rough endoplasmic reticula and mitochondria. The immunreactivities were observed in ribosomes, polysomes, rough endoplasmic reticula and outer membrane of mitochondria. Golgi complexes were poorly developed and not showed jmmunreactivity. The ribosomes , polysomes and endoplasmic reticula are considered to be closely related to the inter cellular localization and biosynthesis of the ChAT but not Golgi complex. According to the results in the present study, it is considered that the ChAT-immunoreacted nerve cells in the Meynert basal nucleus of the rat forebrains are differentiated throughout the postnatal development with following processes of changes; 1) the cholinergic nerve cells develop postnatally 2) cell soma volumes gradually increase during the early postnatal days 3) and then, cells differentiate into the various types by projecting the neurites to the appropriate area after PND 21.
Structural Differentiation of Photosynthetic Tissue in Kranz Anatomy of Salsola Species
Kim, In-Sun ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 367~374
Leaves of two developmental stages of Salsola species, young and mature, were examined to reveal the structural and functional relationships in the photosynthetic tissue using anatomical and ultrastructural criteria. Both young and mature leaves had Kranz anatomy of the Salsolid type with two layers of chlorenchyma on the leaf periphery: an outer layer of palisade mesophyll cells and an inner layer of compact bundle sheath cells with centripetally arranged organelles. The chlorenchyma was continuous in young leaves , while it was discontinuous in mature leaves. The main vascular bundle occupied the central position in the leaf. but the small peripheral vascular bundles were in contact with the chlorenchyma. Structural dimorphism of chloroplasts was obvious in bundle sheath cells of mature leaves exhibiting noticeable grana reduction, whereas mesophyll cell chloroplasts had well developed grana in all cases. Plasmodesmata were less numerous and rather simple in young leaves relative to well-developed secondary plasmodesmata of the later stage. According to the current data, features of two stages of Salsola leaves corresponded to NADP-ME bio-chemical subtype on the basis of photosynthetic cell ultrastructure. Implications of developing such anatomical and ultrastructural data of Sulsola species and biochemical characteristics reported in other C-4 species have been discussed.
Some Observations on the Fine Structure of Korean Bat Livers
Choi, Byung-Jin ; Uhm, Chang-Sub ; Chang, Byung-Jun ; Park, Chang-Hyun ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 375~383
To understand the fine structure of Korean bat livers , the authors investigated the liver of four Korean bats; Rhinolephus ferrumequinum korai, Myotis macrodactylus, Myotis dauhentonii ussrinesis, and Murina leucogaster intermedia by transmission electron microscopy. The hepatocytes of Rhinolophus ferrumequinum korai had large-sized mitochondria and many peroxisomes. In the Myotis macrodactylus, juctional completes, especially desmosomes, were well developed. The Myotis daubentonii ussrinesis had many glycogen particles in the cytoplasm. Also, the space of Disse and sinusoidal spare was filled with amorphous materials. In the Murina leucogaster intemedia, basement membrane was prominent in the sinusoid, and no Kupffer and Ito cells were observed These results suggest the characteristic differences in the liver ultrastructure among Korean bats.
Ultrastructural Study of the Liver by Chitosanoligosaccharide Administrated in Rat
Kim, Young-Ho ; Roh, Young -Bok ;
Applied Microscopy, volume 31, issue 4, 2001, Pages 385~392
The purpose of this study was to evaluate the histological toxicity of chitosanoligosaccharide on the rat. A healthy male of Wistar rat that weighted
was used for experiment The experimental group was divided into five groups. Group 1 was control group which treated with general food Group 2 was
generation which was born by mating of 0.1% (1 mg/ml) chitosanoligosaccharide was supplied by feeding ad libitum for 30 days. Group 3 was
generation which was born by mating
generation. Group 4 was treated with 90 days of 0.1% (1 mg/ml) chitosan oligosaccharide. Group 5 was treated with 365 days of 0.1% (1 mg/ml) chitosanoligo saccharide. All experimental groups were used to 10 rat. The results were as follow: The RER dilation was observed Group 4. However, there were no significantly changes of ultrastructures in the other groups compared to the control. It was concluded that chitosanoligosaccharide can be used for nontoxic natural material.