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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 32, Issue 4 - Dec 2002
Volume 32, Issue 3 - Sep 2002
Volume 32, Issue 2 - Jun 2002
Volume 32, Issue 1 - Mar 2002
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Effect of Electroacupuncture on the Regeneration of Injured Peripheral Nerve
Deung, Young-Kun ; Kim, Dong-Heui ; Kang, Song-Jian ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 1~8
To confirm the effect of electroacupuncture on the regeneration of injured peripheral nerve, the change of evoked potential in the sciatic nerve, the change of enzyme activity in the spinal cord, and morphological change of injured sciatic nerve were examined comparatively in acupuncture group (AG) and control group (CG) after sciatic nerve of guinea pig was injured by purpose. The value of evoked potential after injury of the sciatic nerve was increased in both AG and CG, but the increase rate of that was higher in AG than CG. Acid phosphatase activity of the spinal cord was increased in 1CG and 2AG, but shown are tendency to return to the normal state as time went by. Ultrastructural recovering rate of the injured sciatic nerve was higher in AG than CG. Also, there was developed only adipose tissue in sciatic nerve of AG. As mentioned above, the effect of electroacupuncture on the regeneration of injured peripheral nerve was confirmed experimentally by change of evoked potential, acid phosphatase and ultrastructure. Especially, the effect of electroacupuncture was appeared clearly in an early stage than other treatment stages.
Microstructure Observations in
Lee, Hwack-Joo ; Ryu, Hyun ; Park, Hyun-Min ; Cho, Yang-Koo ; Kim, Jae-Chun ; Nahm, Sahn ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 9~15
Microstructural investigations of
(NACT) complex perovskite compounds were carried out using X-ray diffractometry, neutron diffraction and transmission electron microscopy. When
, NACT had not only the 1 : 1 chemical ordering of cations but also the antiphase and inphase tilting of oxygen octahedron and the antiparallel shift of cations. Both the antiphase boundaries and the ferroelastic domains were present in the microstructure. The long and straight ferroelastic domains became degenerate as x decreases. When x was smaller than 0.3, the chemical ordering was absent and the antiphase tilting of oxygen octahedron was observed. The defects like tangled dislocations and the second phase were also found in the microstructure.
Fine Structure of Retinae of Cephalopods (Todarodes pacificus And Octopus minor) Inhabiting the Korean Waters I
Han, Jong-Min ; Chang, Nam-Sub ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 17~30
The retinae of Todarodes pacificus and Octopus minor are divided into four layers that are an outer segment, a rod base region, an inner segment, and a plexiform layer, respectively. The retina of Octopus minor is about
) than that of Todarodes pacificus (
). A retina is composed of visual cells and supporting cells. The microvilli of length
are packed densely on top of the supporting cells of Octopus minor while they are not found in Todarodes pacificus. The visual cells and supporting cells have pigment granules that exclude light. In case of Todarodes pacificus, the pigment granules of the visual cell are larger (
) than those of the supporting cell (
). But, the sizes of both cells are similar in Octopus minor. In the upper portion of a visual cell, microvilli shaped like a comb are forming a rhabdome (diameter, 60 nm) of a hexagonal structure. The rhabdome consists of 4 rhabdomere and the total area of a rhabdom of Octopus minor is larger than that of Todarodes pacificus. The synaptosome constructing a plexiform layer in Todarodes pacificus are divided into two types, each of which possess electron dense-core vesicles and electron lucent vesicles, respectively. Octopus minor also has two types of synaptosomes but each type comprises a mixture of electron dense vesicles and electron lucent vesicles, and electron lucent vesicles only, respectively, which is different from the case of Todarodes pacificus.
Quasicrystals And Related Approximant Phases in Mg-Zn-Y
Park, Eun-Soo ; Ok, Jae-Bum ; Kim, Won-Tae ; Kim, Do-Hyang ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 31~37
As-cast microstructure of Mg-rich
has been investigated by a detailed transmission electron microscopy. The as-cast
alloy consisted of three different types of phases:
size primary solidification phase, dendritic phase grown from the primary phase and a eutectic structure formed at the later stage of solidification. The primary solidification phase has an icosahedral structure with a large degree of phason strain. 1/1 rhombohedral approximant phase with lattice parameters:
is first observed in Mg-Zn-Y system. The rhombohedral structure can be obtained by introducing phason strain in the six dimensional face centered hyper-cubic lattice. The decagonal phase nucleates with orientation relationship with the icosahedral phase, and
nucleates with orientation relationship with the decagonal phase, indicating a close structural similarity between the three phases. Gradual depletion of Y during solidification plays an important role in heterogeneous nucleation of decagonal and
phases from icosahedral and decagonal phases respectively.
Ultrastructure of the Fertilized Egg Envelope from Dark Sleeper, Eleotrididae, Teleost
Kim, Dong-Heui ; Reu, Dong-Suck ; Deung, Young-Kun ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 39~44
The ultrastructure of the fertilized egg envelope from dark sleeper, Odontobutis obscurus interrupta belong to Eleotrididae was studied using scanning and transmission electron microscopes. The fertilized egg of dark sleeper was of transparent, ellipsoidal, adhesive and demersal type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth with pore canal. The fertilized egg envelope consisted of two layers, an outer nonadhesive layer and an inner layer, consisting of seven horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae. These ultrastructural characters of fertilized egg envelope from dark sleeper can be utilized in taxonomy of teleost.
Ultrastructural Character on the Cuticular Surface of Thelazia callipaeda
Kim, Soo-Jin ; Joo, Kyoung-Hwan ; Chung, Myung-Sook ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 45~55
The worm of Thelazia callipaeda Railliet et Henry, 1910 (The oriental eye worm) was frequently observed in the eyes of animal and human in Korea. But it did not clearly describe about the ultrastructural character on the sensory papillae and cuticular striation of the worm. This study was performed to investigate the ultrastructure and character on the cuticular surface of the worm that was extracted from the eyes of two patients in Korea University Medical Center, using the scanning electron microscopy. According to the mouth, 1/4, 1/2, 3/4 and tail portion of the worm, the size of cuticular striation on each portion was measured. The size of cuticular striation on the worm surface was
in the mouth and tail portion,
in the middle portion of the worm. On the scanning electron microscopy, the female worms were developed phasmids in the tail end and male worms were developed sensory papillae and external sexual organ on the tail end. The sensory papillae on the tail end were composed anterior ventral postcloacal papillae, middle ventral postcloacal papillae, subventral postcloacal papillae, and lateral papillae. According to the result in this study, it is considered that the character of the cuticular striation and the sensory papillae were able to accept as classifying key for the identification of species.
A Study of Structure of the Sucker of Common Freshwater Goby (Rhinogobius brunneus) and Triden Goby (Tridentiger brevispinis)
Kim, Taik-Young ; Son, Sung-Won ; Choi, Byung-Jin ; Park, Chang-Hyun ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 57~66
The structures of sucker of two Cobiidae; Common freshwater goby and Triden goby were observed by light and electron microscopy. Scanning electron microscopy revealed the characteristic narrow ridges and grooves on the apical portion of sucker of Common freshwater goby, and hexagonal structures similar to a honeycomb representing the intercellular junctional area on the middle and basal portions. Some ridges were present on the epithelial surface on the middle and basal portions. The openings of several mucus-secreting cells were present between main epithelial cells. Light and transmission electron microscopy revealed the core of the fin; soft rays with a surrounding dense collagen fiber layer. Some loosely arranged fibers (collagen fiber) radiated toward the surface epithelium. The surface epithelium was cuboidal or columnar in shape. Scanning electron microscopy revealed the coiled irregular ridges and grooves, which was less developed and had sparser distribution than in Common freshwater goby, on the apical portion of sucker of Triden goby. The middle and basal portions had honeycomb structures as in Common freshwater goby. Fewer mucoussecreting cells were present. Light and transmission electron microscopy showed the core of soft rays, dense collagen fiber layer, however, the radiating fibers observed in the Common freshwater goby was rarely present. The sucker was thinner because the epithelium is squamous or polygonal in shape and rare presence of the radiating fibers.
Effects of Deer Antler on the Regeneration of Peripheral Nerves; About Sprout Formation of Experimentally Transected Sciatic Nerves in Rat
Chang, Byung-Joon ; Cho, Ik-Hyun ; Choi, Hye-Young ; Won, Hui-Young ; Park, Chang-Hyun ; Bae, Chun-Sik ; Choe, Nong-Hoon ;
Applied Microscopy, volume 32, issue 1, 2002, Pages 67~80
This study was carried out to investigate the effects of deer antler extract on the regeneration of peripheral nerves. Sprague-Dawley male rats weighing about 300 gm were fed deer antler extract for 1, 2, and 3 weeks per oral (1.5 ml/100 gm B.W.), respectively, once a day and transected both sides of sciatic nerve of each leg. After keeping for 6 hours, sciatic nerves taken from proximal part of transected region were treated with conventional transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows; 1. Sciatic nerves of normal control group were not showing any sprouts and electron dense axolemmal projections were frequently observed. 2. Sciatic nerves of saline treated groups were showing axonal sprouts at the nodes of Ranvier. The length of them was usually short, and numerous vesicles, vacuoles and organelles including neurofilament were contained. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 29 (29%) in 1 week treated group, 32 (32%) in 2 weeks treated group, and 30 (30%) in 3 weeks treated group, respectively. 3. Sciatic nerves of deer antler treated groups were showing axonal sprouts at the node of Ranvier as well. Although most of the sprouts were short, some sprouts of 2 weeks and 3 weeks treated groups were quite long. Sprouts usually contained numerous vesicles, vacuoles and cell organelles such as neurofilaments and mitochondria. The number of nodes of Ranvier containing sprouts from 100 longitudinal sectioned nerve fibers was 38 (38%) in 1 week treated group, 46 (46%) in 2 weeks treated group, and 48 (48%) in 3 weeks treated group respectively. The results described above explain pretreatment of deer antler extract improves the sprout formation of transected sciatic nerves, and then it suggests deer antler may be effective for the regeneration of peripheral nerves.