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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume & Issues
Volume 32, Issue 4 - Dec 2002
Volume 32, Issue 3 - Sep 2002
Volume 32, Issue 2 - Jun 2002
Volume 32, Issue 1 - Mar 2002
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Fresnel Diffraction and Fraunhoffer Diffraction
Lee, Hwack-Joo ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 81~90
In this review, the author discussed how the Fresnel and Fraunhoffer Diffraction can be deduced from the Huygens-Fresnel principle and Kirchhoff Diffraction Theory. Fresnel diffraction became the basic theory of the CTEM image theory, and Fraunhoffer diffraction became the base for electron diffraction and HRTEM image theory by Fourier transformation. The author also discussed the diffraction based on Born series.
Expression of UT-A in Rat Kidney: Ultrastructural Immunocytochemistry
Lim, Sun-Woo ; Jung, Ju-Young ; Kim, Wan-Young ; Han, Ki-Hwan ; Cha, Jung-Ho ; Chung, Jin-Woong ; Kim, Jin ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 91~105
Urea transport in the kidney is mediated by a family of transporter proteins that includes renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). The cDNA of five isoforms of rat UT-A, UTA1, UT-A2, UT-A3, UT-A4, and UT-A5 have been cloned. The purpose of this study was to examine the expression of UT-A (L194), which marked UT-A1, UT-A2 and UT-A4. Male Sprague-Dawley rats, weighing approximately 200 g, were divided into three group: control rats had free access to water, dehydrated rats were deprived of water for 3 d, and water loaded rats had free access to 3% sucrose water for 3 d before being killed. The kidneys were preserved by in vivo perfusion through the abdominal aorta with the 2% paraformaldehyde-lysine- periodate (PLP) or 8% paraformaldehyde solution for 10 min. The sections were processed for immunohistochemical studies using pre-embedding immunoperoxidase method and immunogold method. In the normal rat kidney, UT-A1 was expressed intensely in the cytoplasm of the inner medullary collecting duct (IMCD) cell and UT-A2 was expressed on the plasma membrane of the terminal portion of the shortloop descending thin limb (DTL) cells (type I epithelium) and of the long-loop DTL cells (type II epithelium) in the initial part of the inner medulla. Immunoreactivity for UT-A1 in the IMCD cells, was decreased in dehydrated animals whereas strongly increased in water loaded animals compared with control animals. In the short-loop DTL, immunoreactivity for UT-A2 was increased in intensity in both dehydrated and water loaded groups. However, in the long-loop DTL of the outer part of the inner medulla, immunoreactivity for UT-A2 was markedly increase in intensity in dehydrated group, but not in water loaded group. In conclusion, in the rat kidney, UT-A1 is located in the cytoplasm of IMCD cells, whereas UT-A2 is located in the plasma membrane of both the short-and long-loop DTL cells. Immunohistochemistry studies revealed that UT-A1 and UT-A2 may have a different role in urea transport and are regulated by different mechanisms.
Fine Structural Study of Coelomic Solitary Spermatogenesis in Urechis unicinctus
Shin, Kil-Sang ; Kim, Wan-Jong ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 107~119
Early spermatocytes of U. unicinctus are found in cluster floating in the coelomic fluid. The spermatocytes in a cluster form a syncytium or cytoplasmic mass, but there are no indications that the cytoplasmic mass is a component of a somatic cell. This work suggested that this type of spermatogenesis can be subordinated to solitary spermatogenesis in the sense excluding structural and functional support of a somatic cell for sperm developments. The solitary spermatogenesis in U. unicinctus is different in appearances and developmental details of sperm organelles and stage distributions from that of localized spermatogenesis. The acrosomal rudiments and centrioles can be observed in the early single cells of spermatogonia and clearly disclosed in the primary spermatocyte. In the stage of secondary spermatocyte, the acrosomal precursor and the centrioles begin to move to each cytoplasmic poles. The polarities of the organelles are attained at stage of spermatids. The spermatocytes and spermatids are arranged circumferentially along the cytoplasmic mass in which some amorphological cytoplasmic components are included. The spermatids reveal to be detached from the cytoplasmic mass into coelomic fluid. It suggests that the spermatogenesis are progressed in support of coelomic fluid, and the fact take into consideration that the spermatogenic cells can be in vitro cultured without somatic cells and with supplements of coelomic fluid.
Ultrastructure on the Integumentary Epidermis of the Bastard Halibut, Paralichthys olivaceus (Teleostei: Pleuronectidae)
Kim, Jae-Won ; Chin, Pyung ; Jin, Young-Guk ; Lee, Jung-Sick ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 121~129
The structure of integumentary epidermis is studied in the bastard halibut, Paralichthys olivaceus based on the light and transmission electron microscope. Epidermal layer consists of supporting cells, unicellular glands and accessory cells. The supporting cells were classified into superficial cell, intermediated cell and basal cell. Superficial cell of epidermal layer is squamous or cuboidal and the surface is covered with numerous microridges. The supporting cells are connected to another cell with membrane interdigitations and desmosmes. And tonofilaments are developed in the cortical cytoplasm. Gland cells are classified into mucous cell and club cell. By the histochemical studies of the epidermal secretions the mucous materials are identified as neutral polysaccharides. Club cell has numerous vacuoles and microfilaments in the cytoplasm. Also chloride cells are observed in the epidermis, it cytoplasm is occupied numerous mitochondria.
Fine Structure of Optic Lobes of Cephalopods (Todarodes pacificus and Octopus minor) inhabiting the Korean Waters
Han, Jong-Min ; Chang, Nam-Sub ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 131~147
Optic lobes of Todarodes pacificus and Octopus minor are largely divided into cortex and medulla, the cortex being composed of three layers (an outer granule cell layer, a plexiform layer, and an inner granule cell layer). The cortex of Todarodes pacificus is about
thicker than that of Octopus minor of which thickness is about
. In the outer granule cell layer of Todarodes pacificus, three types of nerve cells (type-A, type-B and type-C) and neuroglial cells that surround or contact with the neurons are observed, while in the outer granule cell layer of Octopus minor, two types of nerve cells (type-A and type-B) and a single type of neuroglial cells are observed. In a plexiform layer, a presynaptic bag and nerve endings are connected to each other, consequently forming various types of synaptosomes. The synaptosomes of Todarodes pacificus contain electron dense vesicles, electron dense-core vesicles and electron lucent vesicles, either individually or in a mixture. On the other hand, three types of synaptosomes a mixture of electron dense-core vesicles and electron lucent vesicles, electron lucent vesicles only, and electron dense-core vesicles only are observed in Octopus minor. The structures of the inner granule cell layer are almost similar in the two species. It is composed of two types of nerve cells (type-A, type-B) and a single type of neuroglial cells. In the medulla of Todarodes pacificus, the cells of
are arranged to a line and form the palisade cell layer, but these are not observed in Octopus minor.
Histological Study on the Activated Carbon in the Rat Liver with Toxicated by Lead
Chung, Min-Ju ; Roh, Young-Bok ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 149~156
For investigation of the activated carbon in the rat liver toxicated by lead. Aniamls used
weeks to Sparague-Dewley rat (150 g). The lead acetate (500 ppm) were injected and activated carbon (40 mg/kg) were treated orally for four and eight weeks, respectivelly, and observed by the electron microscope. The group with only lead for 4 weeks, The mitochondria and rough endoplasmic reticulum (rER) were extended and ribosomes dropped from the rER. The group with lead-activated carbon for 4 weeks, The number of lysosomes increased. The shapes of nucleus and rough endoplasmic reticulum observed almost similar with nomally. The group with only lead for 8 weeks, The mitochondria and rough endoplasmic reticulum (rER) were more extended. The group with lead-activated carbon for 8 weeks, the nucleus and rough endoplasmic reticulum was observed nomally.
Microstructure Observations in Complex Perovskite
Lee, Hwack-Joo ; Ryu, Hyun ; Park, Hyun-Min ; Cho, Yang-Koo ; Nahm, Sahn ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 157~162
Microstructural investigations of
(NPT) complex perovskite compounds were carried out using X-ray diffractometry and transmission electron microscopy. NPT had not 1:1 chemical ordering of Asite cations but had the antiphase and inphase tilting of oxygen octahedron and the antiparallel shift of cations. Both the antiphase boundaries and the ferroelastic domains were not present in the microstructure. Unidentified second phase was found in the microstructure. The measured dielectric properties were
The Effect of Acute Sinusitis on the Ultrastructure and Sialic Acid Distribution on the Sinus Mucosa Cell Surface of the Rabbit
Kim, Soo-Jin ; Lee, Eun-Jung ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 163~170
Experimatal maxillary sinusitis was induced in New Zealand white rabbits by blocking the maxillary sinus ostium. The distribution of lectin receptors was explored in the mucosa with induced maxillary sinusitis using colloidal gold label complex with lectin WGA purified from wheat germ (Triticum vulgaris). The lectin WGA gold complex, shown to recognize GlcNac (N-acetylglucosamine) and NeuNAc (N-acetylneuraminic acid) regions, was applied to detect binding sites in Lowicryl HM 20 sections and viewed under the electron microscope. An increased height of the cylindric cells, ciliary loss and hyperplasia of the secretory cells were observed. Examination of normal sinus mucosa labeled with gold-labeled lectins showed the distribution of sialoglycoconjugates to be mainly in the ciliary layer and the granules in the secretory cells. Inflamed mucosa had increased labeling intensity of gold-labeled WGA in the cilia and the secretory granules. These results indicate that lectin WGA receptors are located in the cilia and secretory granules. Specific changes in the lectin binding pattern were apparent in the inflamed mucosa in the experimentally induced acute sinusitis, in comparison with normal mucosa, conceivably as a part of host defense reactions.
Immuno-Electron Microscopic Studies on the Distribution of Dopamine and
in the Optic lobes of Cephalopods (Todarodes pacificus and Octopus minor) inhabiting the Korean waters
Han, Jong-Min ; Chang, Nam-Sub ;
Applied Microscopy, volume 32, issue 2, 2002, Pages 175~183
In this study, we carried out immunostaining and immunogold labeling with rabbit anti-dopamine (TH) and rabbit anti-calbindin-
to examine the characteristics and functions of the neurons that secrete neurotransmitters in optic lobes of Todarodes pacificus and Octopus minor inhabiting the Korean waters. The obtained results are as follow. In the immunostaining with anti-dopamine, only a few of the large amacrine cells in an the upper part of an outer granule cell layer and the cells forming the islands of medulla showed positive reaction in Todarodes pacificus, while
cells in the upper and middle parts of an outer granule cell layer and more than 5 cells in the islands of medulla reacted positively in Octopus minor. For the case of anti-calbindin case,
small amacrine cells in the upper portion of the outer granule cell layer and
cells which are located in the lower part of an inner granule cell layer showed positive reaction in Todarodes pacificus, while, in Octopus minor, 4 cells in the outer granule cell layer reacted positively, no immunoreactive cell being found in the inner granule cell layer. As a result of performing the immunogold labeling, relative large number (
) of gold particles were labeled per
of the cytoplasm of the cells which showed the immunoreactivity to the anti-dopamine and anti-calbindin in Todarodes pacificus, however, small number (10) of gold particles were labeled in Octopus minor, which reach only half of the number in the Todarodes pacificus.