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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Society of Electron Microscopy
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Volume 32, Issue 4 - Dec 2002
Volume 32, Issue 3 - Sep 2002
Volume 32, Issue 2 - Jun 2002
Volume 32, Issue 1 - Mar 2002
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Crystal Structure Analysis by Texture Electron Diffraction Pattern
Lee, Su-Jeong ; Jou, Hyeong-Tae ; Kim, Youn-Joong ; Moon, Hi-Soo ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 185~193
The works of texture electron diffraction patterns for crystal structure analysis are written in Russian or introduced briefly in books written in English, which makes it difficult to be understood. In addition to working out the equations, vector theory corrects some errors included in the established formulas for texture electron diffraction patterns.
Lee, Hwack-Joo ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 195~204
In this review, the fundamental concepts of delta function, convolution integral and Fourier transformation are discussed. The applications of Fourier transformation to slit function, two very narrow slits, two slits of appreciable width, periodic array of narrow slits, arbitary periodic function, diffraction gratings and gaussian functions are also introduced.
Effects of Chitosan on the Rat Nephrotoxicity Induced by Lead
Kim, Young-Ho ; Roh, Young-Bok ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 205~211
The purpose of this study was to evaluate the effect the lead toxicity of chitosan on the rats. A healthy male of Wistar rat was used for experiment. The lead acetate (30 mg/kg, Pb) was injected two times per a week into the abdomen. 0.1% chitosan solution (1 mg/ml) was supplied by feeding ad libitum. After 4 or 8 weeks, we was observed kidney by electron microscope. The experimental groups were divided into two groups. Group 1 was control group which was not treated with chitosan solution after contaminatation of lead acetate. Group 2 was experimental group which was treated with chitosan solution after contamination of lead acetate. All groups were used to 10 rats. The results were as follow: On the proximal convoluted tubules of the group with only Pb, the number of microvillis were decreased and the number of lysosomes were increased on the cytoplasm. The mitochondria and rough endoplasmic reticulum were extended and ribosomes dropped from the rough endoplasmic reticulum. On the proximal convoluted tubules of the group with Pb-chitosan, the shapes and number of microvilli were not changed and the number of lysosomes were decreased. The shapes of mitochondria and endoplasmic reticulum observed not changes. We concluded that chitosan has effect on reducing toxicity of lead in the rat kidney.
Ultrastructure of the Mantle Epidermis in the Ark Shell, Scapharca broughtonii (Bivalvia: Acridae)
Lee, Jung-Sick ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 213~222
Histology and ultrastructure of the mantle epidermis in the ark shell, Scapharca broughtonii are described using light and electron microscopy. The mantle of the ark shell is composed of outer epidermis, connective tissue and inner epidermis. Both epidermis are simple and consists of supporting cells, ciliated cells and secretory cells. Connective tissue is composed of mainly collagen and muscle fibers. The supporting cells in the inner epidermis are usually columnar and covered with microvilli. The ciliated cell have cilia and microvilli on the free surface, and numerous tubular mitochondria are observed in the apical cytoplasm. Secretory cells are mainly observed in the outer epidermis, and it can be divided into four types of A, B, C and D with morphological features of the secretory granules. Type A cells of mucous cell are found in the marginal and central mantle. And these cells contains numerous secretory granules of non-bounded and low electron density. Type B cells contains numerous rough endoplasmic reticula, well-developed Golgi complex and secretory granules of membrane-bounded and high electron density. Secretory granules of type C cells are divided into fibrous core layer and homogeneous peripheral layer. Type D cells are found in the outer epidermis of the central and umbonal mantle. And secretory granules of these cells are divided into homogeneous core layer and granular peripheral layer. This results suggest that the outer and inner epidermis of the mantle are related with shell formation and cleaning of the mantle cavity, respectively.
on Osteoporosis in Rats
Bae, Chun-Sik ; Choi, Seok-Hwa ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 223~230
Vitamin D is one of important factors involved in the regulation of bone metabolism. In osteoporosis, the therapeutic effect of vitamin D on the healing process has still been controversial. To confirm the effects of
on the osteoporosis, the change of bone mineral density and the histologic changes of osteoporotic femur were examined comparatively in normal control group (positive control), CFA control group (negative control), CFA+
, 25 dihydroxyvitamin
) and CFA+
, 25 dihydroxyvitamin
) after osteoporosis was induced by single injection of complete Freund's adjuvant (CFA) in rats. The value of bone mineral density and bone mineral content of femur was increased in both Vit
than CFA control, and the increase rate of that was higher in Vit
. In CFA control, the size of the bone marrow cavities significantly increased and the width from the bone marrow cavity and cortex significantly decreased than normal control. In Vit
, the increase rate of the size of bone marrow cavity and the decrease rate of the width from the bone marrow cavity and cortex was depressed than CFA control. These results suggest that
has therapeutic effect on adjuvant-induced osteoporosis in rats.
Effects of Squalene on SOD Activity and Histological Changes in Liver Toxicity Induced by Cadmium
Choi, Young-Bok ; Kim, Jong-Se ; Kim, Jung-Sam ; Cho, Kwang-Pil ; Hwang, Koo-Yeon ; Park, Jung-Pyung ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 231~246
This study was carried out to investigate the effect of squalene (SQ) on the mouse hepatotoxicity induced by cadmium. ICR male mouse weighting about 30 gm were injected
(5.0 mg/kg) and SQ (180 mg/kg) into intraperitoneal. At the 1, 2, 3, 4, 5, 6, 7 days, livers were treated with superoxide dismutase (SOD) activity and transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows: SOD activity in the liver, Group A was higher than in normal. Group B was lower than in Group A. In the histological observation, nucleus of Group A showed irregular shape. Inner cavity of mitochondria swellen and development of cristae weakened. Swelling of Lamellae of rough endoplasmic reticulum (RER) showed. Nucleus of group B showed normal shape. Typical lamellae of RER were observed. These results described above treatment of SQ decreased the hepatotoxicity of the
and SOD activity in the mouse liver, and then it suggests SQ may be effective for the recovery of hepatic cell.
Immuno-Electron Microscopic Studies on the Localization of Serotonin and Somatostatin in the Optic Lobes of Cephalopods (Todarodes pacificus and Octopus minor) Inhabiting the Korean Waters
Chang, Nam-Sub ; Han, Jong-Min ; Kim, Sang-Won ; Lee, Kwang-Ju ; Hwang, Sun-Jong ; Lee, Jung-Chan ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 247~255
In this study, we carried out immunostaining and immunogold labeling with antibodies to serotonin and somatostatin to examine the characteristics and functions of the neurons that secrete neurotransmitters in optic lobes of Todarodes pacificus and Octopus minor. As a result of immunostaining with anti-somatostatin, the nerve cells of Todarodes pacificus reacted as similar to the anti-serotonin, but in Octopus minor, only large cells in the outer granule cell layer reacted positively. In the immunogold labeling with anti-serotonin, the nerve cells in the inner grande cell layer and medulla of Todarodes pacificus reacted strongly, 30 gold particles being labeled per
of the cytoplasm. However, in Octopus minor, only 17 gold particles were labeled, which stated a weak reaction. On the other hand, in the anti-somatostatin case, the nerve cells in the outer and inner granule cell layers and medulla of Todarodes pacificus showed strong reaction, 30 gold particles being labeled per
of the cytoplasm while the nerve cells in the outer granule cell layer of Octopus minor reacted weakly, about 3 gold particles being labeled per the equivalent area. As a result of immunostaining and immunogold labeling with two types of antibodies to each part of the optic lobes, we found that the reactive nerve cells were distributed differently in the two species. In particular, the degree of reactivity to the immunostaining and immunogold labeling appeared stronger in Todarodes pacificus than in Octopus minor.
Microstructural Characterizations on
Lee, Hwack-Joo ; Ryu, Hyun ; Park, Hyun-Min ; Cho, Yang-Koo ; Nahm, Sahn ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 257~263
Microstructural investigations of
(LPT) complex perovskite compounds were carried out using X-ray diffractometry and transmission electron microscopy. LPT has not only the ordering of A-site cation deficiencies but also has the antiphase and inphase tilting of oxygen octahedron and the antiparallel shift of cations. Both the antiphase boundaries and the ferroelastic domains are present in the microstructure. Spinodal decomposition is found in the microstructure. The measured dielectric properties were
The Effects of Fluoride Administered Systemically during Rat Development on Forming Bone
Lim, Do-Seon ; Ahn, Yong-Soon ; Kim, Eun-Sook ; Bae, Hyung-Joon ; Chang, Byung-Soo ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 265~273
The purpose of this study was to observe the influences of the water fluoride concentration on the growth changes, the histologic characteristics of osteoblast in the tibia of growing newborn rats by using electron microscopy and on the composition changes of bone matrix in those by using energy dispersive x-ray system (EDX). The water fluoride concentration was respectively 0 ppm (contrast group), 100 ppm (100 ppm group), 200 ppm (200 ppm group) and 300 ppm (300 ppm group). The results of the investigation by using electron microscopy were as followed. In contrast group, the traditional cuboidal osteoblasts were observed. In 100 ppm group, several reversal line, the newly formed osteoid by the strongly activated osteoblast and the well developed rough endoplasmic reticulum, mitochondria in cytoplasm of osteoblast were observed. Also, many secretory vesicle around cell membrane were observed and some fused with cell membrane released secretory granule out of cell. In 200 ppm group, the depressed osteoblasts were observed, mitochondria in cytoplasm were expanded and cristae shape in mitochondria were destroyed. Also, the ribosome at the surface of rough endoplasmic reticulum were not observed. In 300 ppm group, the adjacent osteoblasts with endosteum were irregularly arranged, the cell membrane were destroyed and organelles were flowed out of cell. On the other hand, the results of the investigation by using energy dispersive x-ray system were as followed. P and Ca concentrations in 100 ppm group were increased more than those in contrast group. But, in 200 and 300 ppm group were not increased more than those in 100 ppm group. Therefore, the activities of the osteoblasts were increased, the bone matrix were actively synthesized by the supplied water fluoride. But, the osteoblasts were destroyed, inhibited by the higher water fluoride concentration.
Fine Structural Approach of Granular Gland Regeneration after Skin Injury in Bombina orientalis
Jeong, Moon-Jin ; Lim, Do-Seon ; Moon, Myung-Jin ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 275~284
Granular gland regeneration in the toad after dorsal skin wound histologically was examined using scanning and transmission electron microscopy. After cutaneous wounds were induced by excision, animals were maintained in special cages for up to 20 days. In transmission electron microscopy (TEM), newly formed granular gland, though poorly developed, was seen on 4 day after injury. Epithelial cells moved toward apical region of newly formed gland. The cells had smooth surface and were not connected to other cells by desmosomes. Mitochondria rich cell (MRC) possessing long cytoplasmic processes formed a gland cavity and hemidesmosomes were found under the cell processes. Basal cavity of newly formed gland consisted of MRC, pro-granular producing cells (pGPC), and granular producing cell (GPC). Moreover it was observed that xanthophores moved to the base of the epithelial tissue on 10 day after the injury. These cells contained numerous pterinosomes and carotenoid vesicles. Immature pterinosomes were large and carotenoid vesicles were moderately electron dense. On 13 day after the injury, xanthophores contained abundant carotinoid vesicles and lammelated pterinosomes. Iridophores were also observed adjacent the developing xanthophores on 16 day post-injury. These observations indicated that regeneration of granular gland from glandular precursor cells during wound healing and subsequent expansion of the glandular cells might be dependent on maturation and proliferation of these newly formed cells.
Therapeutic Effects of Safflower (Carthamus tinctorius L.) Seed Powder on Osteoporosis
Bae, Chun-Sik ; Park, Chang-Hyun ; Cho, Hyung-Jin ; Han, Hye-Jeong ; Kang, Seong-Soo ; Choi, Seok-Hwa ; Uhm, Chang-Sub ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 285~290
The therapeutic effect of safflower seed powder on experimental osteoporosis in the rat induced by ovariectomy was evaluated. Thirty Sprague-Dawley rats were ovariectomized at the age of 12 weeks. Seven weeks postovariectomy, rats were divided into two groups: control and safflower seed powder treated group. Five animals from each group were sacrificed at the following time points: 1, 3, and 5 weeks. Scanning electron microscopic observation and morphometric analysis of the tibiae epiphysis showed that the administration of safflower seed powder significantly prevented reduction of cortical bone width and bone volume compared with the control group. In conclusion, safflower seed powder contains something that prevent bone loss due to estrogen deficiency, and was effective in preventing the osteoporotic decrease of bone mass.
Morphological Differences of the Flight Muscle among Xylocopa appendiculata circumvolans Smith, Davidins lunatus B. and Serrognathus platymelus castanicdor M.
Moon, Hye-Jung ; Ban, Young-Hun ; Cho, Hyun-Gug ; Park, Won-Hark ; Lee, Jong-Wook ;
Applied Microscopy, volume 32, issue 3, 2002, Pages 291~301
The present study was performed to compare the morphological differences of flight muscles among 3 species from insects (Xylocopa appendiculata circumvolans Smith, Davidins lunatus B. and Serrognathus platymelus castanicdor M.) by investigating ultrastructural observation and stereological analysis. Xylocopa appendiculata circumvolans Smith has the most flight hours. In addition, the number and arrangement of mitochondria and the structure of sarcomere were similar to those of vertebrates. However sarcomere structure of Davidins lunatus B. was irregular and the sarcomere length was longer than that of Xylocopa appendiculata circumvolans Smith. In Serrognathus platymelus castanicdor M. which has the least flight hours, the length of sarcomere appeared longer than that of Davidins lunatus B. In results of stereological analysis, Serrognathus platymelus castanicdor M. had the highest volume density of myofibrils in all species. The volume and numerical density of mitochondria and the volume density of sarcoplasmic reticulum were highest Xylocopa appendiculata circumvolans Smith and Davidins lunatus B. respectively. This study suggests that the flight hours and flight pattern by different ecological habitats may cause the morphological changes of flight muscle.