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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 32, Issue 4 - Dec 2002
Volume 32, Issue 3 - Sep 2002
Volume 32, Issue 2 - Jun 2002
Volume 32, Issue 1 - Mar 2002
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Spermatogenesis and Sperm Ultrastructure of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae)
Park, Chae-Kyu ; Park, Jung-Jun ; Lee, Jeong-Yong ; Lee, Jung-Sick ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 303~310
Spermatogenesis and sperm ultrastructure are investigated by means of light and transmission electron microscopy in the equilateral venus, Gomphina veneriformis which is dominant bivalve in the east coast of Korea. In the active spermatogenic season, testis consists of numerous spermatogenic follicles which is contains germ cells in the different developmental stage. The spermatogonia attached to spermatogenic follicle wall and has a large nucleus with electron-dense nucleolus. The spermatocytes are characterized by appearance of synaptonemal complex and well-developed Golgi complex. Nucleus of spermatid consists of numerous heterogeneous granules with high electron density. Karyoplasmic condensation, acrosome and flagellum formations are observed during spermiogenesis. Testicular matured sperms of sperm bundle consists of head, midpiece and tail. The head is about
long and comprises a long nucleus and a bullet-like acrosome (
in length). Acrosomal rod of microfilaments is observed in the lumen between nucleus and acrosome. The midpiece has four mitochondria. And tail has the typical '9+2' microtubule system.
TEM Specimen Preparation Method of Gibbsite Powder for Quantitative Structure Analysis
Kim, Young-Min ; Jeung, Jong-Man ; Lee, Su-Jeong ; Kim, Youn-Joong ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 311~317
There is great requirement on the TEM specimen preparation method with particle size selectivity as a prerequisite for the quantitative structure analysis on the materials such as gibbsite powder, which generally forms a large agglomerate and shows a variation of transition process depending on their sizes. In this experiment, we made an attempt to give a methodology for the TEM specimen preparation of powder with the size selectivity. After mixing 1 wt% gibbsite powder with ethanol solvent, gibbsite suspension was prepared by application of ball-milling and ultrasonification with addition of 0.25 vol% dispersion agent, Darvan C, which was diluted into distilled water by the ratio 1:19. Appling the static sedimentation method to gibbsite suspension after estimation of the sedimentation time by the measurement of accumulative concentration variation, we acquired TEM specimens with well-dispersed and size selected gibbsite particles in nm scale. Overall picture of each sample was taken by SEM and morphology of each dispersed particle was imaged by TEM. Raw and processed gibbsite powders were also examined by XRD to investigate whether they were suffered from phase change during the process or not.
Structural Features of Various Trichomes Developed in Salvinia natans
Ji, Sang-Yong ; Kim, In-Sun ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 319~327
Salvinia natans, an unique water fern having a small rootless body, developed three different types of trichomes throughout the plant. The most peculiar type exhibiting rows of obvious, whitish, multicellular trichome clusters was noticed in the upper surface of the floating leaves. Eight to ten branches within a cluster extended ca.
from the leaf surface. No stalk cell was found, however, four large epidermal cells were discernable at the base of four central branches in the cluster. Each branch consisted of
obliquely-oriented small cells that gradually decreased in size toward the branch tip. The second type was found in the lower surface of the floating leaves, stems, and sporocarps. Multicellular uniseriate trichomes, ca.
long, were distributed all over these structures. The tip of trichome was acicular, but a semi-spheric protuberance of approximately
in diameter occurred at the base of each trichome. The protuberance appeared to be firmly attached to the side of the basal cell, however, internal connection to the trichome cell itself was uncertain. The third type was similar to the second in that multicellur uniseriate trichomes with acicular tip and a protuberance at the base were present. However, the trichomes were considerably long relative to the second type, and only occurred along the surface of highly dissected, submerged leaves. A majority of the trichomes exceeded more than 2 mm in length that hung downward in the water. Regardless of trichome type, all trichomes contained a huge central vacuole with very thin cytoplasm, resulting from the fusion of several vacuoles during early trichome development. The various densely-distributed trichomes formed in Salvinia natans probably play an important role in plant buoyancy.
Morphological and Cellular Characteristics of Aerial Roots in the Epiphytic American Ivy (Parthenocissus sp.)
Yim, Ji-Eun ; Kim, In-Sun ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 329~337
The morphology and cellular characteristics of adventitious roots, viz aerial roots, in the epiphytic American Ivy were examined to reveal structural changes of the aerial root upon surface attachment. Immature aerial roots were composed of parenchyma cells with dense cytoplasm containing plastids, however, the upper and lower epidermis were not distinguished. At early development, electron-dense substances (EDS) were constituents of much of the aerial root tissue, but the distribution of EDS varied within the tissue. The deposits appeared most concentrated in the superficial cell layers, with lesser amounts in cell layers closer to the cortex. Electron micrographs revealed that EDS deposits were always found in the vacuole, and were mainly associated with the tonoplast. While most of them occurred in the vacuole as small spherical deposits adjacent to the tonoplast, some deposits were oddly shaped or larger in size. Many of the vacuoles eventually filled with EDS, but the EDS content in those vacuoles decreased substantially after initial attachment to the surface. When the vacuoles became almost empty, cells near the epidermis already exhibited irregularity in outline. Subsequent breakdown of cellular components took place in the cells while they were still attached to the surface. This study suggests the potential role of EDS as substances involved in the surface attachment of the plant, however, further studies must be conducted to reveal the nature of EDS and the effects of EDS storage within these vacuoles.
Structural Development of Scalp Hair in Children and Effects of Cosmetologic Physico-Chemical Treatments on Adult Scalp Hair
Park, Chang-Hyun ; Choi, Ki-Ju ; Moon, Sung-Jin ; Uhm, Chang-Sub ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 339~344
Recently, there has been an increase in the cosmetologic treatments on hair in Korea. To investigate the time when morphological characteristics of human scalp hair take mature form, hairs of 0, 3, 6, 12, 24 months, and of adult scalp hair were compared. Also, the physico-chemical effects of commonly used cosmetological treatments; hair-dryed, dye coated, stained and permanent waved scalp hairs were observed by scanning electron microscopy. Two year-old hair was similar to the adult hair, and all hairs received cosmetologic treatments except for coated hair showed various degree of deformation and damage. These results suggest that routinely used cosmetologic treatments may give harmful effects on hairs. More detailed studies in large scale may be necessary.
A Palynotaxonomic Study of the Korean Crassulaceae
Sin, Jin-Hwan ; Yoo, Young-Gi ; Park, Ki-Ryong ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 345~360
Pollen morphology of four genera, 22 species of Korean Crassulaceae has been examined with light and scanning electron microscopy. The presence/absence of the aperture margo and granules was useful characters to clarify the generic boundaries, and the shapes of the streate were significant to delineate taxonomic relationships within the genus Sedum. The pollen grains of Korean Crassulaceae were small and medium in size. Hylotelephium viviparum has the smallest pollen grains, and H. viridescens the largest. The pollen grains are tricolporate, circular in polar view and were prolate to prolate-spheroidal in equatorial shape. Korean Crassulaceae species have striate grains. The close pollen morphological relationships among Hylotelephium, Meterostachys and Orostachys species were concordant to the results based on the previous molecular and morphological studies. The results of the clustering analysis using pollen characters support the segregation of Sedum sensu lato which has been proposed by the previous authors. Within the genus Sedum, S. kamtschatium is closely related to S. middendorffianum with granules on the surface of the pollen grains. Sedum polytrichoides, a member of the Sedum, was clustered to the species of the Meterostachys, Hylotelephium and Orostachys, and needs to reexamine the taxonomic status. O. malacophyllus collected at Chungdong-Jin was significantly differ from the same species from Gampo in pollen and morphological characters.
Effects of Chitosanoligosaccharide on the Mouse Hepatotoxicity Induced by Cadmium
Yoon, Jung-Sik ; Roh, Young-Bok ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 361~376
This research was conducted to determine the effects of chitosanoligosaccharide on liver poisoning induced by cadmium (Cd). Three groups of mice were used in this research. The group was only injected with cadmium (5.0 mg/kg; i.p.) (group Cd) and the other group was injected with cadmium and chitosanoligosaccharide (0.5% solution) at the same time (group Cd+Chi). In order to investigate the inhibitory action of chitosanoligosaccharide on liver damage, cadmium concentration in liver tissues and metallothionein (MT) concentration were relatively measured. In addition, histological observations were made to determine the morphologic injury of liver tissues. Cadmium concentration in liver tissues was drastically lower in groups Cd+Chi than in group Cd. MT concentration in liver tissues was lower in group Cd than in groups Cd+Chi. As the result of electron microscopic observation, mitochondria in group Cd showed a severe swelling phenomenon, RER fragment and ribosome dropout. However, in groups Cd+Chi, mitochondria with high electron density were distributed and RER forming a typical lamellae with ribosome was observed. From these results, cadmium toxicity on rat liver tissues could be lessened by chitosanoligosaccharide.
Ultrastructural Localization of ZnT3 and Zinc Ions in the Mouse Choroid Plexus
Kim, Sung-Joo ; Kim, Yong-Kuk ; Sun, Yuan-Jie ; Kim, Soo-Jin ; Jeong, Young-Gil ; Yu, Yun-Cho ; Jo, Seung-Mook ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 377~383
We have detected the murine zinc transporter, ZnT3, and zinc ions in the mouse choroid plexus by immunocytochemistry (ICC) and zinc selenium autometallography (
), respectively. BALB/c mice served as experimental animals. Routine floating ABC immunocytochemical procedures were used for the ZnT3 immunocytochemistry, and the mice were injected intraperitoneally (i.p.) with sodium selenide (10 mg/kg) for the zinc selenium autometallography. The choroid plexus showed weak immunoreactivity (Ir) for ZnT3. At high magnification, ZnT3-Ir was seen to be located in the choroid epithelium and the connective tissue of the capillaries. At the EM level, a high electron density of ZnT3-immunoreactivity was restricted to vesicle membranes as well as microvilli in the apical membrane. In contrast, immunostaining of ZnT3 was completely absent in the basolateral plasma membrane and other cell organelles. After silver enhancement, fine
grains were observed in both the epithelial and endothelial cells of the choroid plexus. Few
grains present in the cell bodies of the choroid epithelial cells were located in multivesicular bodies. It is striking that very many
grains were observed in the endothelial cells of the capillaries. These findings establish the choroid plexus as a non-neuronal pool of zinc ions in the brain, although the functional significance of this pool is not clear. The choroid epithelium, however, may play an important role in the transportation of zinc between the CSF and brain tissue.
Laminin-1 Expression in Bone Marrow Stromal Cells of Cyclophosphamide-treated Rat
Lee, Chang-Hun ; Chung, Ho-Sam ; Paik, Doo-Jin ; Hwang, Se-Jin ; Kim, Won-Kyu ; Youn, Jee-Hee ; Kim, Chong-Kwan ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 385~398
The purpose of the present study is to investigate whether stromal cells supporting specific microenvironment for hematopoiesis of bone marrow are affected by toxicants and therapeutic drugs such as antibiotics and anticancer drugs and whether laminin-1 is associated with such effects. SD rats were intraperitoneally injected with 75 mg/kg of cyclophosphamide which is widely used to treat infant's solid tumor, leukemia and myeloma and sacrificed after 3 days, 1 week, 3 weeks or 5 weeks of injection. The bone marrow extracted and paraffin-sectioned was analyzed using immunohistochemical staining. A part of tissues was subjected to electron microscopy following reaction with rabbit anti-laminin antibody, biotinylated goat anti-rabbit IgG conjugated with 12 nm gold particles, and staining with uranyl acetate. 1. The bone marrow tissue at day 3 post injection with cyclophosphamide displayed dilated venous sinus, partial necrotic death, and decreased number of hematopoietic cells. Laminin-1 was intensively stained in the reticular and adipose tissues. 2. Up to 5 weeks post injection, laminin-1 was stained at a low level in the stromal tissue of bone marrow and the number of hematopoietic cell was increased. 3. Deposition of the gold particle which represents laminin-1 expression was observed at the highest level in the stromal cells of bone marrow obtained 3 days after injection, and decreased after 1 to 5 weeks. These results suggest that stromal cells which play a role in supporting microenvironment for bone marrow hematopoiesis augment induction of laminin-1 expression and activation upon administration of cyclophosphamide.
Changes of the Antioxidant Enzymes and Histopathological Changes of Liver and Kidney According to the Administered Dosage of Bambusae Caulis in Liquamen in Mice
Kim, Jeong-Sang ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 399~410
This study was performed to investigate the effects of Bambusae Caulis in Liquamen (bamboo extract) on the changes of antioxidant enzymes and histopathological changes of liver and kidney in mice when administered in different dosages. The experimental groups were divided into four. For control group, 0.9％ NaCl (0.2 ml/25 g B.W.) and for experimental groups, 5% (H1 group), 10% (H2 group), and 20%(H3 group) bamboo extract diluted with 0.9％ NaCl, were administered (0.2 ml/25 g B.W.) respectively for 28 days at an interval of 48 hours. MnSOD activities were increased in H1 group (46%, P<0.05), H2 group (40%, P<0.05), and H3 group (34%, P<0.05) as compared to the control group. CuZnSOD activities were increased in H1 group (11%, P<0.05), but were decreased in H3 group (13%, P<0.05). The activities of catalase were decreased in H1 group (39%, P<0.05), H2 group (34%, P<0.05), and H3 group (31%, P<0.05) as compared to the control group. Histopathological observation revealed ballooned hepatocytes in the pericentral and periportal veins of H1 group. More ballooned and injured hepatocytes than in H1 group were observed in the H2 and H3 groups. Detachment of endothelial cells of the central vein was observed in the H2 and H3 groups. These results indicated that bamboo extract developed dose-dependent changes in antioxidant enzyme activities and developed histopathological changes of liver and kidney.
Immunocytochemical Localization of Metallothionein in Gastric Adenocarcinoma
Yang, Seung-Ha ; Shin, Kil-Sang ; Kim, Wan-Jong ;
Applied Microscopy, volume 32, issue 4, 2002, Pages 411~419
Metallothionein (MT) is a family of ubiquitous, low molecular weight (
), cysteine-rich (
) inducible protein with a high affinity to metal ions and has no aromatic amino acids and histidine. Some of the known functions of MT include detoxification of heavy metals and alkylating agents and neutralization of free radicals. Also, this protein has been reported to involve in tumor pathophysiology and therapy resistance. MT expression may affect a number of cellular processes including gene expression, apoptosis, proliferation and differentiation. Many reports on the physiological and biochemical properties of MT have been published, but ultrastructural reports on the localization of MT in human gastric cancer tissues are extremely rare. The present study was undertaken to examine the ultrastructural features and the localization of MT within the gastric adenocarcinoma. Ultrastructures of gastric cancer cells were characterized by the high nuclear cytoplasmic ratio, the interdigitation between cells, the irregular nucleus containing much heterochromatin and the wide distribution of free ribosomes in the cytoplasm. Immunohistochemical reaction for MT was prominent in the gastric adenocarcinoma. And the immunogold labellings were more prominent within the nucleus than the cytoplasm. Particularly, immunogold particles were numerously seen at nulcleolus or nucleolar associated heterochromatin. These results suggest that MT expression by gastric cancer cells is associated with cell proliferative activity and is possibly synthesized in the cytoplasm, and then the protein is transported into the nucleus to participate in any transcriptional steps.