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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 33, Issue 4 - Dec 2003
Volume 33, Issue 3 - Sep 2003
Volume 33, Issue 2 - Jun 2003
Volume 33, Issue 1 - Mar 2003
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Theory of Imaging And Diffraction
Lee, Hwack-Joo ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 169~178
In this review, theoretical approaches of imaging and diffraction in electron microscopy are introduced which allows the diffraction patterns and images to be treated with equal facility and emphasized the relationships between them. The coherent wave optics, incoherent wave imaging theory were introduced. The idea of Abbe theory was also introduced. Varoius phase contrast theories in small angle approximation were derived including the wave theory on Multi-component system.
A Method to Determine the Wavelength of Electron Beam from LACBED Pattern
Kim, Hwang-Su ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 179~185
The operating accelerating voltage in the electron microscopy may differ from the nominal voltage specified by the manufacture. Thus it is necessary, at least once, to determine the wavelength of electron beam for the nominal accelerating voltage. Particularly in QCBED technique, the wavelength of the incident electron beam on a specimen must be determined as accurately as possible. In this paper we present a simple method to determine accurately the wavelength of electrons from LACBED patterns of a known crystalline materials, which is analogous to a method based on Kikuchi patterns reported previously. This method is to utilize three diffraction lines not belonging to the same zone, which nearly intersect at the same point. For an application of the method, the wavelength of electrons for the 200 kv nominal acceleration voltage of JEM2010 is determined to be 0.002496(3) nm (
kv) with an uncertainty of 0.12%.
A Simple Method to Determination the Rotation Angle Between an Image and its Diffraction Pattern with LACBED Patterns
Kim, Hwang-Su ; Kim, Jong-Pil ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 187~193
When electron microscope images and selected area diffraction patterns of crystalline materials are being compared, it is important to know for the rotation of the diffraction pattern with respect to the image caused by the magnetic lens in the Electron Microscope. A well-known method to determine this rotation is to use a test crystal of
. But this method of determination of the rotation angle contains an uncertainty of
. Thus one has to devise another way to eliminate this uncertainty. In this paper we present a new and simple method of determining this rotation without any complexity. The method involves a process of obtaining LACBED patterns of crystalline materials. For the J2010 electron microscope, the rotation is determined to be
and this angle remains unchanged for changing of the magnification and the camera length.
Accurate Interpretation of Electron Diffraction Data Acquired by Imaging Plates
Kim, Young-Min ; Kim, Youn-Joong ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 195~204
The Experimental calibration method has been investigated to correct d-spacing estimation and to identify phases in the electron diffraction data acquired by imaging plates. When the diffraction data from the imaging plate was corrected by the d-spacing calibration method with the radial intensity distribution plotting in this experiment, The accuracy of d-spacing estimation was significantly increased in errors of about 0.5%. The experimental calibration equation followed up the first order exponential decay function was derived from the trace of d-spacing deviation between the measured and the calculated values. It was applied to the analysis of d-spacing and the phase identification of the transitional phases formed from  gibbsite specimen by electron beam irradiation effect. In this case more accurate phase identification and d-spacing evaluation is possible for the transitional phases whose diffraction patterns are complicatedly superimposed. It is concluded that
-alumina are clearly identified as the major transitional phases formed from gibbsite by electron beam irradiation for 120 min.
Effects of Squalene on the Mice Hepatotoxicity Induced by Lead Acetate
Kim, Jong-Se ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 205~214
To investigate the effect of the squalene against the lead toxicity. A healthy male of ICR mice were used for experiment. The SOD was observed after the intraperitoneal injection in mice. The ultrastructural changes of the liver were observed after the intraperitoneal injection in mice. The experimental groups were divided into two groups. Group A was control group that squalene was not treated after intraperitoneal injection of lead acetate. Group B was squalene treatment group that squalene solution was injected after intraperitoneal injection of lead acetate. All groups were used to 10 mice. The results were as follow: SOD activity in the liver, Group A was lower than in normal. But, Group B was higher than in Group A (P<0.05). In the histological observation, Group A were showed that the inner cavity of mitochondria swellen and development of cristae weakened. Swelling of lamellae of rough endoplasmic reticulum (rER) was showed. It was concluded that the SQ will be effective for the recovery of hepatic cell at lead intoxication.
Fine Structure of Damaged Hair Shaft by Daily Treatment of Heat for a Beautiful Face
Chang, Byung-Soo ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 215~222
The ultrastructure of damaged hair shaft from blow-drying at typical temperature
for daily beautiful face have been investigated by using transmission electron microscope and scanning electron microscope. When we used to hair dryer for a long time in our everyday life, the following morphological alternations were found in hair. First, the partial of scales in outer cuticle were detached simultaneously with separation of intercellular membrane complex of cuticle cells. Then hair broke cuticle off and exposed to cortex. Secondly, the cortical cell in the cortex was fissured into its macrofibril. The melanin granules were scattered between macrofibrils. As a result, I confirmed that blow-drying removed the hair's bonded water and made hard on hair which lost elasticity. After all, hair showed irregular, rough surface and vanished its luster.
Gill Ultrastructure of the Granular Ark, Tegillarca granosa (Bivalvia: Acridae)
Ma, Kyung-Hwa ; Lee, Jung-Sick ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 223~231
Histology and ultrastructure of the gill in the granular ark, Tegillarca granosa are described using light and transmission electron microscopy. The gill of the clam have typical structure of the filibranch type. The gill filament have several band of lateral and apical cilia. The epithelial layer surrounding the hemolymph sinus is simple and consists of epithelial cells, ciliated cells and secretory cells. The epithelial cells are usually squamous and covered with microvilli. The ciliated cells are usually columnar and can be divided into two types (A and B). Type A cells are more abundance and have lower electron density than B cells. Ultrastructure of the cilia showed that '9+2' microtubular structure of the axial filament and '
' proximal centriole structure in the cross section. Secretory cells are mainly observed in the apical region of the filament and can be divided into three types of A, B and C with morphological features of the secretory granules. Type A cells of oval shaped are more abundance than other secretory cells and contains numerous secretory granules of low electron dense. Type B cells contains secretory granules of membrane-bounded and high electron dense. Secretory granules of type C cells are elliptical and fine granules surrounding the homogeneous core materials.
Patterns of Tannin Accumulation in Leaves of C-4 Euphorbia maculata
Kim, In-Sun ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 233~241
Patterns of tannin accumulation in leaves of C-4 Euphorbia maculata have been examined using electron microscopy. Tannins, which are secondary metabolite phenolic compounds, were found to be deposited conspicuously in vacuoles of certain tissues regardless of their stage in development. However, patterns of deposit accumulation were distinguishable by their cell type during leaf differentiation. The deposits appeared most concentrated in the concentric bundle sheath cells enclosing veins, while little or no density was detected mostly in the mesophyll cells close to the epidermis. An ultrastructural study revealed that the deposits were restricted to the vacuoles at an early stage of leaf development; during which the vacuoles were almost completely filled with the tanniferous substances. The deposits themselves took different forms ranging from granules to huge globules while expanding leaf blade. As the leaf matured, the deposits accumulated either centripetally adjacent to the inner tangential tonoplast or by penetration into the cytoplasm amongst various cellular organelles, resulting in an extremely dense cytoplasm. Electron micrographs frequently showed the delineation of each organelle by the presence of dense deposits within the cytoplasm. Some large depository vacuoles filled with tannins had a corrugated appearance on the sectioned surface. The pattern and potential role of the deposits have been discussed.
Ultrastructural Study on the Development of Male Germ Cell of the Olive Flounder, Paralichthys olivaceus (Teleostei: Pleuronectidae)
Kim, Jae-Won ; Kim, Bong-Seok ; Choi, Cheol-Young ; Lee, Jung-Sick ;
Applied Microscopy, volume 33, issue 3, 2003, Pages 243~250
Ultrastructural changes of the male germ cells and structure of spermatozoa in Paralichthys olivaceus were examined by means of the light and transmission electron microscopes. The spermatogonium has a large nucleus with a single nucleus with a single nucleolus in the interphase. Primary spermatocytes are identified by the formation of the synaptonemal complex in the karyoplasm. The secondary spermatocytes are more concentrated and contains numerous cell organelle in the cytoplasm. The nucleus of spermatid in spermiogenesis is more condensed in the karyoplasm, and show spherical structure in shape. Mitochondria of the spermatids are observed in the lower portion of the nucleus. The spermatozoon consists of the head, mid piece and tail. The acrosome is not observed in the head. Axial filaments of the flagellum consists of nine pairs of the peripheral microtubules and one pair of the central microtubules.