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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 33, Issue 4 - Dec 2003
Volume 33, Issue 3 - Sep 2003
Volume 33, Issue 2 - Jun 2003
Volume 33, Issue 1 - Mar 2003
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Radiations and Their Scattering by Matter
Lee, Hwack-Joo ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 251~259
In this review, the sources and the characteristics of X-rays and electrons and their interactions with matters were described in terms of the atomic scattering factors. The geometrical diffraction conditions were taken into account in terms of Ewald spheres in reciprocal lattice spaces. The effects of the finite size of sources and detectors on diffractions were also considered.
Effect of Porous Membrane on Culture Properties of Blood-Brain Barrier Endothelial Cell
Lee, Keum-Jeong ; Cho, Hye-Jin ; Choi, Hyung-Taek ; Na, O-Soon ; Kim, Kyung-Yong ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 261~266
The growth patterns of primary culture of bovine brain microvessel endothelial cells (BBMECs) were studied using electron microscopy when grown on
pore Transwell. The capillary fragments and isolated endothelial cells grew on collagen coated culture plate and Transwell membrane. The BBMECs grew only on the upper surface of membrane of
. But BBMECs on
pore membrane migrated through the pore and grew on the opposite side of the membrane. In summary, BBMECs isolated by enzyme digestion could migrate through
pore membrane but not through
pore membrane. So
pore membrane instead of
pore membrane should be used for drug transport experiment or transendothelial electrical resistance measurement.
An Ultrastructural Study on the Spermatogenesis of Xiphophorus maculatus
Kim, Dong-Heui ; Reu, Dong-Suck ; Deung, Young-Kun ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 267~274
The ultrastructures of spermatogenesis and sperm in Xiphophorus maculatus, ovoviviparous fish were investigated by electronmicroscopy The testis of Xiphophorus maculatus contained numerous testicular sacs, and spermatogenesis was synchronized in these testicular sac. In the case of spermatogonium, the nucleus was comparatively large ellipsoidal, and the nucleolus and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged along the tail. The sperm was formed by loss of cytoplasm. The head of mature sperm was long cone shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the sperm has a loop-like structure at the end of a tail.
Effects of Colchicine on Pulmonary Injury Induced by Paraquat
Kim, Joong-Kil ; Chang, Moo-Ung ; Kwak, Jyung-Sik ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 275~282
This study was undertaken to investigate paraquat-induced pulmonary injuries and effects of colchicine on pulmonary fibrosis by paraquat. Fifteen Sprague-Dawley rats were intraperitoneally injected 10 mg/kg of paraquat and repeatedly with 2 days interval. Another 15 rats were injected paraquat as same manner and simultaneously injected 10 mg/kg of colchicine in a week. Five rats in each group were sacrificed 1, 2, and 4 weeks after initial injections, and lungs extracted were observed by light and electron microscopes. On light microscopy, there was mild infiltration of neutrophils, macrophages, and lymphocytes in alveolar spaces and walls at 1 week after paraquat injection. The cellularity of alveolar wall was increased with time. However, the cellularity was not so prominent in paraquat and colchicine simultaneously injected group. On electron microscopy, there was marked swelling or excoriation of type I epithelial cells and alveolar capillary endothelium with infiltration of neutrophils, macrophages and monocytes, and lymphocytes in alveolar walls. Such findings were persisted with time. In addition, fibroblastic proliferation and deposition of collagen fibers were prominent at 4 weeks after paraquat injection. Fibrosis also occurred at 4 weeks after paraquat and colchicine simultaneous injection. It was not proninent than that of paraquat injected group. According to the above result, it would be concluded that the type I pneumocytes and alveolar capillary endothelial cells are most vulnerable on paraquat poisoning, and that the colchicine is effective on inhibition of paraquat-induced pulmonary fibrosis.
Effects of High-salt Diet on the Mouse Adrenal Medulla
Moon, Young-Wha ; Kang, Wha-Sun ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 291~298
Exposure to stressful stimuli is known to activate the peripheral sympathetic nervous system and the adrenal gland. In this study, we evaluated the effects of high-salt diet on the mouse adrenal medulla using the tyrosine hydroxylase (TH) immunohistochemistry and the transmission electron microscopic observation. Immunoreactivity for TH was increased after high-salt diet. Especially, the TH immunoreactivity was stronger in 4 days high-salt diet mouse than that of 4 weeks. TH immunoreactivity was mainly present in the cytoplasm and granules of the noradrenaline cells. After high-salt diet, the noradrenaline cells exhibited the ultrastructural alterations consisting of areas of empty cytoplasm, expanded granules, and some damaged mitochondria. These results suggest that high-salt diet may be a factor of stressful stimuli on the mouse.
The Role of Protein Kinase C in the Cardiac Injury Induced by Skin Burn
Moon, Hye-Jung ; Cho, Hyun-Gug ; Park, Won-Hark ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 299~313
The aim of the present study was to assess the role of protein kinase C (PKC) in the development of cardiac injury following scald burn. Sprague-Dawley rats were induced a scald burn a 15% total body surface area. Phorbol 12-myristate 13-acetate (PMA, 2 mg/kg) and bisindolylmaleimide (BIS, 0.05 mg/kg) were immediately administered i.p. after burn injury. 5 h and 24 h later, heart was removed and examined biochemical assay, ultrastructural changes and stereological analysis. The activity of serum aspartate aminotransferase was significantly increased at 5h (p<0.01) and 5h+BIS (p<0.001) after burn compared with that of control. The activity of serum creatinine was significantly decreased in PMA-treated groups after burn compared with postburn 5 h. PMA caused a decrease in MPO activity and induced wavy fibers in cardiac myocytes at postburn 5 and 24h. BIS induced contraction band, separation of intercalated disk and abnormal mitochondria in cardiac myocytes at postburn 5 and 24h. In stereological analysis, treatment of rats with PMA increased volume density of myofibril and mitochondria compared with postburn 5 and 24h. Our data suggest that the activation of PKC in scald burned heart decreases inflammation and protects the myocardium.
Ultrastructure of the Spinnerets and Spigots in the Funnel-web Spider, Agelena limbata
Moon, Myung-Jin ; Kang, Chang-Soo ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 315~323
The fine structural characteristics of the spinnerets and spigots of the silk producing apparatus in the adult funnel-web spider, Agelena limbata, were analysed with the light and scanning electron microscopes. Silk producing apparatus of this spider was composed of three pairs of spinnerets (anterior, median, posterior) and four different types of spigots-ampullates, tubuliforms, pyriforms and aciniforms. By the examination of their ultrastructural characteristics, it has been revealed that each spigot on the spinnerets are connected through the typical silk gland within abdominal cavity. Among the three pairs of spinnerets, the posterior pairs were highly elongated and has most characteristic features. Two pairs of large ampullates were connected to anterior spinneret and another two pairs of small ampullates to median spinnerets. Spigots of the tubuliforms were observed only in female and were connected both of median and posterior spinnerets respectively. While spigots of the pyriforms were connected on the anterior spinnerets but aciniforms on both of median and posterior spinnerets respectively.
Fine Structure of the Heart Tube and Its Cardiac Muscle Cells in the Spider, Araneus ventricosus
Choi, Jae-Young ; Moon, Myung-Jin ;
Applied Microscopy, volume 33, issue 4, 2003, Pages 325~333
Fine structural characteristics of the heart tube and its cardiac muscle cells in spider, Araneus ventricosus are investigated by both of scanning and transmission electron microscopes. The heart tube of the spider is extended mid-dorsally along the anterior part of the abdomen, and is consisted of the thin outer layer of connective tissue (epicardium) and the thick muscle layer (myocardium). The myocardium in the spider has a typical fanlike spiral structure toward anterior part put across between the muscle fibers. Therefore, it did not give rise to the intima, and muscle cells are in direct contact to the hemolymph. The heart tube appeared to be three pairs of ostia and numerous hemocytes accumulated at the inner surface of the myocardial layer. Among several kinds of the hemocytes, the oenocytoids are the most predominant hemocytes accumulated along the myocardial folds which stretched toward heart lumen. The heart muscle cells are cross striated, branched, and multinucleated. They contain a lot of mitochondria, which provide for the continuous energy demands of the heart. Thread-like ganglion on the dorsal side of the heart tube gives off axons that innervate the heart muscle cells.