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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
Korean Society of Electron Microscopy
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Volume & Issues
Volume 34, Issue 4 - Dec 2004
Volume 34, Issue 3 - Sep 2004
Volume 34, Issue 2 - Jun 2004
Volume 34, Issue 1 - Mar 2004
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Scattering from Assemblies of Atoms
Lee, Hwack-Joo ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 211~221
In this review, the kinematical scattering theorems are explained by means of Fourier transformation of the assemblies of atoms. The reciprocal space and the generalized Patterson function are introduced. The scatterings of real monatomic gas or liqiud and the hydrogen atoms are described as examples.
Ultrastructural Analysis of Chemical Synapses in Cultured Wild Type Drosophila Embryonic Neurons
Oh, Hyun-Woo ; Park, Ho-Yong ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 223~230
To identify the structural basis of mutations that affect synaptic transmission we have begun quantitative ultrastructural descriptions of synapses in cultured Drosophila embryonic neurons. In wild-type cultures, synapses are distinguished by the parallel arrangement of a thickened pre- and post synaptic membrane separated by a synaptic cleft. The presynaptic active zones and postsynaptic densities are defined by electron dense material close to the membrane. Presynaptic regions are also characterized by the presence of one or more electron dense regions, presynaptic densities, around which a variable number of small, clear core synaptic vesicles (mean
nm in diameter) are clustered. Subsets of these vesicles are in direct contact with either the presynaptic density or the membrane and are considered morphologically docked. A small number of larger, dense core vesicles are also observed in most presynaptic profiles.
The Changes of Occludin in Tight Junction of Blood-Brain Barrier by ROS
Lee, Hee-Sang ; Kim, Dae-Jin ; Sohn, Dong-Suep ; Jeong, Bong-Su ; Choi, Hyung-Taek ; Sim, Kyu-Min ; Lee, Keum-Jeong ; Cho, Hye-Jin ; Kim, Suk-Joong ; Lee, Jong-Chan ; Jeong, Yoon-Hee ; Kim, Sung-Su ; Lee, Won-Bok ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 231~239
Cerebral microvessel endothelial cells that form blood-brain barrier (BBB) have tight junction for maintaining brain homeostasis. Occludin, one of tight junction protein, is crucial for BBB function.
induced occludin changes and effects in bovine brain BBB endothelial cells were examined in this study. The decrease of transendothelial electrical resistance (TEER) by
was due to disruption of occludin localization. Cytotoxicity test revealed that
did not cause cell death below 1 mM
within 4 hr.
caused intermittent disruption and loss of occludin at tight junctions and occludin disappeared with dose dependent manner from tight junction in confocal laser microscopy. But Western blot revealed that the total amounts of occludin increased by
administration. Transmission electron microscopy revealed that the ultrastructure of tight junction was not changed by
. These data suggest that functional disruption of BBB by
was due to the localized loss of occludin in tight junction, but the expression of occludin increased in order to compensate the disrupted function in BBB.
Effects of Squalene on The Epidermal Growth Factor (EGF) Expression and Histological Changes by Glycerol-Induced Acute Renal Failure in Mice
Choi, Young-Bok ; Kim, Young-Ho ; Lee, Jun-Heung ; Kim, Jong-Se ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 241~254
Kidney had recovery functions against toxicants, ischemia, reperfusion-induced damage, acute-renal failure (ARF). Urinary epidermal growth factor (EGF) is produced by the juxtaglomerular apparatus. Kidney accumulates or excretes the EGF. In case of renal diseases, excreted EGF was decreased. The aim of this study is to evaluate the effects squalene (SQ) on the prevention of experimental acute renal failure induced by glycerol. In case of in vitro study, we investigated the expression of EGF by RT-PCR. After the proximal tubular cells was isolated, glycerol (1, 2, 4 mM) or glycerol plus squalene (0.1, 0.05 or 0.1%) was added. In case of in vivo study, we investigated the changes of BUN, creatine, and ultrastructure. Experimental groups were divided into four groups. Group 1 was normal mouse. Group 2 was injected with SQ only (180 mg/kg). Group 3 was not treated with squalene after intraperitoneal contamination of glycerol (50%, 8 ml/kg). And, Group 4 was treated with squalene (180 mg/kg) after intraperitoneal contamination of glycerol (50%, 8 ml/kg). All groups were used to 7 mice. In the results, we investigated the glycerol induced renal failure. The expression of EGF mRNA was decreased in renal proximal tubules when treated with only glycerol. SQ increased the mRNA expression of EGF in renal proximal tubules. SQ also quickly recovered the levels of BUN and creatine compared with those of mice treated with only glycerol (P<0.01). In case of ultrastructure, group 3 had heavily damaged mitochondria, but, mitochondria in group 4 had evidences of the recovery. It was concluded that SQ had the recovery effects for the glycerol-induced acute renal failure.
Three-dimensional Structure Analysis of
by Transmission Electron Microscopy
Kim, Jin-Gyu ; Kang, Sung-Kwon ; Kim, Wan-Cheol ; Kim, Youn-Joong ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 255~264
The three-dimensional (3D) structure of an inorganic crystal,
(space group P4/nmm,
) was solved by electron crystallography. High resolution electron microscopy (HREM) images from 3 different major zone axes and selected-area electron diffraction patterns from 16 different zone axes were combined to obtain a 3D information. A crystallographic image processing (CIP) of HREM images was used for more accurate determination of the crystal structure. As a result of this electron crystallography, average phase errors (
) of ,  and  HREM images are
, respectively. Xray crystallography of
has attempted to compare accuracy of the structure determination by electron crystallography, which resulted in the cell parameters of
, and the R-factor (
) of 4.16%.
Ultrastructural Description of Some Wood Degrading Fungi at Light Microscopic and Scanning Electron Microscopic Levels
Lee, Yang-Soo ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 271~276
The genus of Biscogniauxia, well known wood degrading fungi, is a member of the Xylariales, which has woody to carbonaceous, brown to dark brown stromata. Daldinia concentrica and Biscogniauxia sp. isolated from heavily decayed hardwood, are precisely described under light microscopic and scanning electron microscopic level. Daldinia concentrica will be the first ultrastructural description in Korea. The unidentified species collected, having small size of ascospores
with full germ slit, are taxono-mically compared with similar species found in Korea.
Electron Microscopic Study of Osseointegration between Bone and Smooth Machined Implants
Chang, Byung-Soo ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 277~283
This paper reports that the ultrastructural nature of the interface process between the implants and surrounding bone has been studied after in vivo 1, 4, 8, 12 weeks of implantation of smooth machined implants into rabbit tibias. There was no indication of the fibrous connective tissue formation around the implant that imply intolerance of the bone tissue towards the implant after 1 week of implantation. The regions showing direct bone tissue bonding to the smooth machined implant contained osteoblast activating across the interface in the direction after 4 weeks of implantation. The reaction of a smooth machined implant caused in the first instance formation of an amorphous woven bone, which transformed into a mineralized bone containing collagen fibers. After 8 weeks of implantation, the activities of osteoblast initiated osseointegration forming bone matrix at the interface. During this period, the osteoblast surrounded with a matrix consisting of collagen bundles running in various directions. In the interface area between newly formed bone tissue and implants which has been inserted in rabbit tibias for 12 weeks, the implant and mineralized bone was separated by an amorphous electron dense material layer about
Morphology and Synaptic Connectivity of Cholinergic Amacrine Cells in the Mouse Retina
Kang, Wha-Sun ; Chun, Myung-Hoon ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 285~294
We investigated the morphology, distribution and synaptic connectivity of cholinergic neurons in the mouse retina by immunocytochemistry, using antisera against choline acetyltransferase (ChAT). ChAT-immunoreactive amacrine cells fall into two groups according to the localization of their somas in the retina: one is situated in the inner nuclear layer (INL), near the border of the inner plexiform layer (IPL), and the other is displaced in the ganglion cell layer (GCL). The dendrites of amacrine cells from the INL ramify in sublamina a and that of the displaced amacrine cells ramify in sublamina b of the IPL. Double labeling with an antisera against ChAT and r-aminobutyric acid (GABA) demonstrated that these labeled cells formed a subpopulation of GABAergic amacrine cells. The synaptic connectivity of ChAT-immunoreactive amacrine cells was identified in the IPL by electron microscopy. The most frequent synaptic input of ChAT-labeled amacrine cells was from bipolar cells in both sublaminae a and b of the IPL, followed by labeled amacrine cells and unlabeled amacrine cells. Their primary output targets were onto ganglion cells in both sublaminae a and b and output onto ganglion cells was more frequently observed in sublamina b of the IPL. Our results suggest that cholinergic amacrine cells in the mouse retina are very similar to their counter parts in other mammals, and they can attribute a major role in the pathway feeding into directionally selective ganglion cells.
Development of Epidermal Idioblasts in the Reproductive Structures of Lycopersicon esculentum
Park, Eun-Hee ; Kim, In-Sun ;
Applied Microscopy, volume 34, issue 4, 2004, Pages 295~303
Plants of Lycopersicon esculentum, containing various organic compounds, are known to develop idioblasts in their epidermis. Lycopersicon esculentum have long been investigated in many areas, but structural aspects of the epidermis of various organs have not been carried out in detail. Thus, the present study attempted to reveal the patterns of idioblast development, particularly those of the reproductive organs, in L. esculentum epidermis using scanning electron microscopy. The present study mainly focused on patterns of the stomata and trichome types. Two types of stomata were developed in the flowers and fruits: anomocytic stomata (stomata type I) were distributed normally throughout the epidermis, whereas actinocytic raised stomata (stomata type II) were found variously in different epidermal tissues. For the trichomes, both glandular and non-glandular types were developed in the epidermis. The former included peltate glandular trichomes having four head cells (trichome type I) and capitate multicellular glandular trichomes (trichome type II). The latter included non-glandular short trichomes (trichome type III) and considerably elongated trichomes with basal rosette cells (trichome type IV). In paticular, the raised stomata were well-developed in the peduncles and the peltate glandular trichomes were prominent in the sepal and ovary epidermis. Transmission electron microscopy on the ontogeny and ultrastructural differentiation of these idioblasts, associated with the current result, will aid us in better understanding of the structure and functional relationship in the epidermal differentriation of Lycopersicon esculentum.